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The map label for this gene is cls [H]

Identifier: 116493565

GI number: 116493565

Start: 1822843

End: 1824306

Strand: Reverse

Name: cls [H]

Synonym: PEPE_1839

Alternate gene names: 116493565

Gene position: 1824306-1822843 (Counterclockwise)

Preceding gene: 116493566

Following gene: 116493564

Centisome position: 99.56

GC content: 36.0

Gene sequence:

>1464_bases
ATGTTTCAAATTATAAAAGATATTATATATATTATTCTTATTCTAAATATTATTGGTGCCGTTTATACAGTTTTTAGACA
ACGTCGAGATATAGCTGCTACTTGGGCCTGGTTATTGGTCTTATTTTTCTTGCCTGGTGTAGGCTTTGTGATATACGCCT
TTGTGGGACGAAAGCTACCAGAAAACAAATTATTTCCGCTGAAAAGTAAGACTCGCCTTCAATTGGATCAAGTTATGGAG
CATCAGCGTCGAGAGCTAAAAAAGAATGAGTTTACTGCTGCCGATCAAGTTGTTAAAGATTCAGAACGAATGGTCCAATT
CTTTACTAAAACGGATTATTCTTTTTTAAATCGAAGAAATGAAGTTCAAATCATCACAGATGGAAATGAACTTTTTGATG
ATATTTTTGCTGAAATTAAAAAAGCGAAAAAGCATATTCACGTTGAATTTTATACAATTTATGATGATCAAATTGGCAAT
CAGTTACGCGAGCTATTGGAAGAAAAAGCACGTGAAGGTGTTGAAGTTAAGGTGCTATGGGATTCATGGGGATCGCTAGG
TACCAAAAGAAGTTTCTTCAAGAAATTGAATGAACTAGGCGGAGAAGCGTATCCATTTCTAGGTACACGTTCAGCTTTAA
CTGACTTTCGATTGAACTTCAGGGATCACCGTAAAATTGTCGTGATTGATGGTAAGATTGGTTATGTTGGTGGTTTTAAT
GTGGGAGATCAATATTTAGGCCGTAAGAAGAAATTTGGTTATTGGCGAGATACCCATTTGAAAATCATCGGTTCTGGAGT
TTACGGACTTCAATCAAGATTTATCCTGGATTGGAATGCTACAGACCGTAAAAAACAAATTGTCGAAGAGTTTAAAGCTG
GATCAGTTTATTTCCCAGTCGCTGAAGTTAAAGGCAATACCAGTCTTCAAATCGTTAGTAATGGGCCAGATTCAGATATT
GAAAAAATTAAAATGGGTTATTTGAAAATGATTCATAAAGCCAGAGAATCGGTTTGGATTCAATCACCATATTTAATTCC
AGATGATAGTGTCTTGGACGCATTGCGCTTAGCAGCAACATCGGGAGTAGATGTTCGAGTAATGATTCCATGTATGCCTG
ATCACGCGTTTGTCTATCGTGCAACCCAATATTATGCTTGGCAATGTGCTAAGTGGGGAATTAAAGTTTATTACTACAAC
AATGGTTTCATCCATGCTAAAACGATGGTAGTGGATGGAAAAATATCTACAGTGGGTTCTGCGAATATGGATTTCCGTAG
TTTCAAACTTAATTTTGAAGTTAACGCCTTTATGTATGATGAAAATATTGCAAAACAACTGCAGGAAATTTATGAAGAAG
ATATCAAACATTCTAGTCTCCAAACCGTAGCCATGTTTAATAAACAATCCACATGGCTGAAGTTCAAACAAACCTTTTCA
CGTTTGCTTTCACCAATTTTGTAA

Upstream 100 bases:

>100_bases
GGTAAAATTTAGTCCTAAAAAAAGGCTAAAATAAAAAAGTTCTTTCATTTTTAAATCTACATAGTATCATGAATCCATAC
GAGTATAATGGGGGATTTAG

Downstream 100 bases:

>100_bases
GTAAAAAGAGTTGACGTTATTTTTAACCCGTGATAATCTTGTAAAAAATCAATTAAAGCATTTTAACTTTCACATTAAGT
AGTGCATAATCTGGGTGTCA

Product: cardiolipin synthetase 2

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MFQIIKDIIYIILILNIIGAVYTVFRQRRDIAATWAWLLVLFFLPGVGFVIYAFVGRKLPENKLFPLKSKTRLQLDQVME
HQRRELKKNEFTAADQVVKDSERMVQFFTKTDYSFLNRRNEVQIITDGNELFDDIFAEIKKAKKHIHVEFYTIYDDQIGN
QLRELLEEKAREGVEVKVLWDSWGSLGTKRSFFKKLNELGGEAYPFLGTRSALTDFRLNFRDHRKIVVIDGKIGYVGGFN
VGDQYLGRKKKFGYWRDTHLKIIGSGVYGLQSRFILDWNATDRKKQIVEEFKAGSVYFPVAEVKGNTSLQIVSNGPDSDI
EKIKMGYLKMIHKARESVWIQSPYLIPDDSVLDALRLAATSGVDVRVMIPCMPDHAFVYRATQYYAWQCAKWGIKVYYYN
NGFIHAKTMVVDGKISTVGSANMDFRSFKLNFEVNAFMYDENIAKQLQEIYEEDIKHSSLQTVAMFNKQSTWLKFKQTFS
RLLSPIL

Sequences:

>Translated_487_residues
MFQIIKDIIYIILILNIIGAVYTVFRQRRDIAATWAWLLVLFFLPGVGFVIYAFVGRKLPENKLFPLKSKTRLQLDQVME
HQRRELKKNEFTAADQVVKDSERMVQFFTKTDYSFLNRRNEVQIITDGNELFDDIFAEIKKAKKHIHVEFYTIYDDQIGN
QLRELLEEKAREGVEVKVLWDSWGSLGTKRSFFKKLNELGGEAYPFLGTRSALTDFRLNFRDHRKIVVIDGKIGYVGGFN
VGDQYLGRKKKFGYWRDTHLKIIGSGVYGLQSRFILDWNATDRKKQIVEEFKAGSVYFPVAEVKGNTSLQIVSNGPDSDI
EKIKMGYLKMIHKARESVWIQSPYLIPDDSVLDALRLAATSGVDVRVMIPCMPDHAFVYRATQYYAWQCAKWGIKVYYYN
NGFIHAKTMVVDGKISTVGSANMDFRSFKLNFEVNAFMYDENIAKQLQEIYEEDIKHSSLQTVAMFNKQSTWLKFKQTFS
RLLSPIL
>Mature_487_residues
MFQIIKDIIYIILILNIIGAVYTVFRQRRDIAATWAWLLVLFFLPGVGFVIYAFVGRKLPENKLFPLKSKTRLQLDQVME
HQRRELKKNEFTAADQVVKDSERMVQFFTKTDYSFLNRRNEVQIITDGNELFDDIFAEIKKAKKHIHVEFYTIYDDQIGN
QLRELLEEKAREGVEVKVLWDSWGSLGTKRSFFKKLNELGGEAYPFLGTRSALTDFRLNFRDHRKIVVIDGKIGYVGGFN
VGDQYLGRKKKFGYWRDTHLKIIGSGVYGLQSRFILDWNATDRKKQIVEEFKAGSVYFPVAEVKGNTSLQIVSNGPDSDI
EKIKMGYLKMIHKARESVWIQSPYLIPDDSVLDALRLAATSGVDVRVMIPCMPDHAFVYRATQYYAWQCAKWGIKVYYYN
NGFIHAKTMVVDGKISTVGSANMDFRSFKLNFEVNAFMYDENIAKQLQEIYEEDIKHSSLQTVAMFNKQSTWLKFKQTFS
RLLSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=478, Percent_Identity=25.9414225941423, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1787007, Length=365, Percent_Identity=27.3972602739726, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 56570; Mature: 56570

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQIIKDIIYIILILNIIGAVYTVFRQRRDIAATWAWLLVLFFLPGVGFVIYAFVGRKLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
ENKLFPLKSKTRLQLDQVMEHQRRELKKNEFTAADQVVKDSERMVQFFTKTDYSFLNRRN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
EVQIITDGNELFDDIFAEIKKAKKHIHVEFYTIYDDQIGNQLRELLEEKAREGVEVKVLW
CEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEE
DSWGSLGTKRSFFKKLNELGGEAYPFLGTRSALTDFRLNFRDHRKIVVIDGKIGYVGGFN
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCEEEECCCC
VGDQYLGRKKKFGYWRDTHLKIIGSGVYGLQSRFILDWNATDRKKQIVEEFKAGSVYFPV
CCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEE
AEVKGNTSLQIVSNGPDSDIEKIKMGYLKMIHKARESVWIQSPYLIPDDSVLDALRLAAT
EECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHC
SGVDVRVMIPCMPDHAFVYRATQYYAWQCAKWGIKVYYYNNGFIHAKTMVVDGKISTVGS
CCCEEEEEEECCCCCCEEEEHHHHHEEEEEECCEEEEEEECCEEEEEEEEECCCEEECCC
ANMDFRSFKLNFEVNAFMYDENIAKQLQEIYEEDIKHSSLQTVAMFNKQSTWLKFKQTFS
CCCCEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHH
RLLSPIL
HHHHCCC
>Mature Secondary Structure
MFQIIKDIIYIILILNIIGAVYTVFRQRRDIAATWAWLLVLFFLPGVGFVIYAFVGRKLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
ENKLFPLKSKTRLQLDQVMEHQRRELKKNEFTAADQVVKDSERMVQFFTKTDYSFLNRRN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
EVQIITDGNELFDDIFAEIKKAKKHIHVEFYTIYDDQIGNQLRELLEEKAREGVEVKVLW
CEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEE
DSWGSLGTKRSFFKKLNELGGEAYPFLGTRSALTDFRLNFRDHRKIVVIDGKIGYVGGFN
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCEEEECCCC
VGDQYLGRKKKFGYWRDTHLKIIGSGVYGLQSRFILDWNATDRKKQIVEEFKAGSVYFPV
CCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEE
AEVKGNTSLQIVSNGPDSDIEKIKMGYLKMIHKARESVWIQSPYLIPDDSVLDALRLAAT
EECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHC
SGVDVRVMIPCMPDHAFVYRATQYYAWQCAKWGIKVYYYNNGFIHAKTMVVDGKISTVGS
CCCEEEEEEECCCCCCEEEEHHHHHEEEEEECCEEEEEEECCEEEEEEEEECCCEEECCC
ANMDFRSFKLNFEVNAFMYDENIAKQLQEIYEEDIKHSSLQTVAMFNKQSTWLKFKQTFS
CCCCEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHH
RLLSPIL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]