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The map label for this gene is pdhC [H]

Identifier: 116493499

GI number: 116493499

Start: 1745323

End: 1746612

Strand: Reverse

Name: pdhC [H]

Synonym: PEPE_1771

Alternate gene names: 116493499

Gene position: 1746612-1745323 (Counterclockwise)

Preceding gene: 116493500

Following gene: 116493498

Centisome position: 95.32

GC content: 40.85

Gene sequence:

>1290_bases
TTGACTGAAATTTTTAAGATGCCAGATATTGGCGAAGGAATGGCTGAAGGCGAAATTGCAAACTGGTTAGTAAAAGTCGG
CGATACGATTAAAGAAGAAGATGCAGTAGCTGAAGTTCAAAATGATAAGCTATTACAAGAAATCCTTTCTCCTTATGGTG
GAAAGATTACAAAACTTTTCGTAGAAGCGGGAACGGTAGTTAAGGTTGGCGAACCATTGATTGAATTCGACGGTGACGGA
AGTGGCGCTGGTGCTGAAAGTGAAGTACCTAAAGAAACACCAGCATCTACAGAACCGGAACCAGAATCAAGCGCACCAGT
TGACCAAACTGCACCTGAAGTAACCAAGGTAGGTGCAGAATATACTTCAAATGGACAATTGTTAGCAATGCCATCTGTAC
GAGAATATGCTAGAAAAAATGATATTGATTTAACTCAGGTGCCAGCAACTGGACGGCATGGACATATCACCATGGCTGAT
GTGGAGAACTTTAAAGCAAGTCCTGCCCCAGCTGCTTCAGTTCCAGAAACAGAATCAGAAAAGGCGCCAAGCGCCCCAGT
TACTCCGGCTGCGCCAGCTGAAGTTAAAGCTGGACGGGTACCATTGTCACCAGTTCGTAAAGTAATTGCTAAGACTTTAA
CTAACCAAGTTCAAACGATTCCACATGTAACAATCATGGATGAAGTAGAAGTTTCTAAGTTAATGGATCTTCGTAACCAA
TTTAAGGAACAAGCTAAACAAAAAGGCTACAAACTAACATATATGCCATTTATTGCGAAAGCCCTTGCTGGGGCAGCCCA
TAAGTACCCTGAACTTAGTGCAATGGTTGATATTGAAAAACAAGAAATCGTTTATTACGAAGATACAAATGTCAGTTTCG
CAGTTGATACTGATAATGGACTTTTCGTACCAAACGTTAAGAATGTTAAATCAAAATCAATTATGGAAGTTGCGCAAGAA
ATTGATGACATGGCCATCCGTGGTCGTGCCGGTGATTTGAAGCCTAATGAACTTAAGGGTGGCACAGTTACTATCACCAA
TATTGGTTCAGAAAGTGGAAGTGGATTCTTTACACCAATTATTAATCCAGGTGAAAGTGCAATTCTTGGAATTGGACGAA
TTCGTAAGACTCCAGTGGTTAACGAAGATGGTGAGTTAGCAGTTGGAAATACATTAAAACTATCACTTAGCTTTGATCAT
CGTTTAATTGACGGTGCTCTAGCACAAAAAATTATGAATGAGCTGAAGGCACTTTTGAGTAACCCTGCCTACATGCTAAT
GGAGGTGTAG

Upstream 100 bases:

>100_bases
GTAGCTGCTCCAGATTCAGTATATCCATTTGCCCTTGCTGAAAATGAATGGTTACCAAAAGCAGACGATATCATCGAAAA
AGTTAAGGAGGTCGTGAACT

Downstream 100 bases:

>100_bases
TCATGGTAGTTGGTACACAAGCAATTAACGTTGACACTTTAATTATTGGATCTGGTCCTGGTGGATACGTTGCTGCTATT
CGAGCAGCTGAATTAGGACA

Product: pyruvate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTEIFKMPDIGEGMAEGEIANWLVKVGDTIKEEDAVAEVQNDKLLQEILSPYGGKITKLFVEAGTVVKVGEPLIEFDGDG
SGAGAESEVPKETPASTEPEPESSAPVDQTAPEVTKVGAEYTSNGQLLAMPSVREYARKNDIDLTQVPATGRHGHITMAD
VENFKASPAPAASVPETESEKAPSAPVTPAAPAEVKAGRVPLSPVRKVIAKTLTNQVQTIPHVTIMDEVEVSKLMDLRNQ
FKEQAKQKGYKLTYMPFIAKALAGAAHKYPELSAMVDIEKQEIVYYEDTNVSFAVDTDNGLFVPNVKNVKSKSIMEVAQE
IDDMAIRGRAGDLKPNELKGGTVTITNIGSESGSGFFTPIINPGESAILGIGRIRKTPVVNEDGELAVGNTLKLSLSFDH
RLIDGALAQKIMNELKALLSNPAYMLMEV

Sequences:

>Translated_429_residues
MTEIFKMPDIGEGMAEGEIANWLVKVGDTIKEEDAVAEVQNDKLLQEILSPYGGKITKLFVEAGTVVKVGEPLIEFDGDG
SGAGAESEVPKETPASTEPEPESSAPVDQTAPEVTKVGAEYTSNGQLLAMPSVREYARKNDIDLTQVPATGRHGHITMAD
VENFKASPAPAASVPETESEKAPSAPVTPAAPAEVKAGRVPLSPVRKVIAKTLTNQVQTIPHVTIMDEVEVSKLMDLRNQ
FKEQAKQKGYKLTYMPFIAKALAGAAHKYPELSAMVDIEKQEIVYYEDTNVSFAVDTDNGLFVPNVKNVKSKSIMEVAQE
IDDMAIRGRAGDLKPNELKGGTVTITNIGSESGSGFFTPIINPGESAILGIGRIRKTPVVNEDGELAVGNTLKLSLSFDH
RLIDGALAQKIMNELKALLSNPAYMLMEV
>Mature_428_residues
TEIFKMPDIGEGMAEGEIANWLVKVGDTIKEEDAVAEVQNDKLLQEILSPYGGKITKLFVEAGTVVKVGEPLIEFDGDGS
GAGAESEVPKETPASTEPEPESSAPVDQTAPEVTKVGAEYTSNGQLLAMPSVREYARKNDIDLTQVPATGRHGHITMADV
ENFKASPAPAASVPETESEKAPSAPVTPAAPAEVKAGRVPLSPVRKVIAKTLTNQVQTIPHVTIMDEVEVSKLMDLRNQF
KEQAKQKGYKLTYMPFIAKALAGAAHKYPELSAMVDIEKQEIVYYEDTNVSFAVDTDNGLFVPNVKNVKSKSIMEVAQEI
DDMAIRGRAGDLKPNELKGGTVTITNIGSESGSGFFTPIINPGESAILGIGRIRKTPVVNEDGELAVGNTLKLSLSFDHR
LIDGALAQKIMNELKALLSNPAYMLMEV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=34.4036697247706, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI31711992, Length=449, Percent_Identity=30.0668151447661, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=28.3185840707965, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=28.3185840707965, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=33.6206896551724, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI260898739, Length=140, Percent_Identity=35, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=31.0588235294118, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1786305, Length=433, Percent_Identity=30.9468822170901, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=35.7142857142857, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=29.9776286353468, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=430, Percent_Identity=31.1627906976744, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=229, Percent_Identity=31.4410480349345, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=29.5555555555556, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320352, Length=426, Percent_Identity=30.7511737089202, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=35.4312354312354, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=33.7662337662338, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=33.7662337662338, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=34.4978165938865, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46059; Mature: 45928

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIFKMPDIGEGMAEGEIANWLVKVGDTIKEEDAVAEVQNDKLLQEILSPYGGKITKLF
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VEAGTVVKVGEPLIEFDGDGSGAGAESEVPKETPASTEPEPESSAPVDQTAPEVTKVGAE
EECCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
YTSNGQLLAMPSVREYARKNDIDLTQVPATGRHGHITMADVENFKASPAPAASVPETESE
CCCCCCEEECCCHHHHHHHCCCCEEECCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCC
KAPSAPVTPAAPAEVKAGRVPLSPVRKVIAKTLTNQVQTIPHVTIMDEVEVSKLMDLRNQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
FKEQAKQKGYKLTYMPFIAKALAGAAHKYPELSAMVDIEKQEIVYYEDTNVSFAVDTDNG
HHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCHHEEECCCCEEEEEECCCEEEEEECCCC
LFVPNVKNVKSKSIMEVAQEIDDMAIRGRAGDLKPNELKGGTVTITNIGSESGSGFFTPI
EECCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEC
INPGESAILGIGRIRKTPVVNEDGELAVGNTLKLSLSFDHRLIDGALAQKIMNELKALLS
CCCCCCCEEECCHHCCCCCCCCCCCEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHC
NPAYMLMEV
CCCEEEEEC
>Mature Secondary Structure 
TEIFKMPDIGEGMAEGEIANWLVKVGDTIKEEDAVAEVQNDKLLQEILSPYGGKITKLF
CCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VEAGTVVKVGEPLIEFDGDGSGAGAESEVPKETPASTEPEPESSAPVDQTAPEVTKVGAE
EECCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
YTSNGQLLAMPSVREYARKNDIDLTQVPATGRHGHITMADVENFKASPAPAASVPETESE
CCCCCCEEECCCHHHHHHHCCCCEEECCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCC
KAPSAPVTPAAPAEVKAGRVPLSPVRKVIAKTLTNQVQTIPHVTIMDEVEVSKLMDLRNQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
FKEQAKQKGYKLTYMPFIAKALAGAAHKYPELSAMVDIEKQEIVYYEDTNVSFAVDTDNG
HHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCHHEEECCCCEEEEEECCCEEEEEECCCC
LFVPNVKNVKSKSIMEVAQEIDDMAIRGRAGDLKPNELKGGTVTITNIGSESGSGFFTPI
EECCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEC
INPGESAILGIGRIRKTPVVNEDGELAVGNTLKLSLSFDHRLIDGALAQKIMNELKALLS
CCCCCCCEEECCHHCCCCCCCCCCCEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHC
NPAYMLMEV
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA