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The map label for this gene is pdhD [H]

Identifier: 116493498

GI number: 116493498

Start: 1743914

End: 1745320

Strand: Reverse

Name: pdhD [H]

Synonym: PEPE_1770

Alternate gene names: 116493498

Gene position: 1745320-1743914 (Counterclockwise)

Preceding gene: 116493499

Following gene: 116493497

Centisome position: 95.25

GC content: 39.59

Gene sequence:

>1407_bases
ATGGTAGTTGGTACACAAGCAATTAACGTTGACACTTTAATTATTGGATCTGGTCCTGGTGGATACGTTGCTGCTATTCG
AGCAGCTGAATTAGGACAAAAAGTTGTGATTGTTGAACGTGATTATATCGGAGGAGTTTGTTTGAATGTTGGATGTATTC
CATCTAAAGCTTTGATTCAAGCTGGACACCTCTACTCAACTTTACAACATGGTAATCCATTTGGAGTTTCGGTATCAGAA
TCTAAAATTGATTTCACTAAAACTCAAGAATGGAAACAAAATCAGGTTGTTAACAAACTAACTGGTGGAGTGGAACTCTT
ACTTAAGAAACACAAAGTTGCAATTGTGCGAGGAGAAGCTTACTTCAATGATAACGAAACGGTTAACGTAATCAACGGAG
ATGATTCCCATGTATATCGCTTTAAGAGAGCTTTGATTGCCACTGGTTCTCGTCCAGTTGAAATCCCACACTTTAAATTT
AGTGGTCGAGTAATTGATTCCACTGGAGCCTTGAACTTAAAAGATGTTCCAGAACATCTCATCATTATTGGTGGTGGGGT
GATTGGAGCGGAACTTGCTGGAGCTTACATGAACCTTGGTTCAAAGATTACCATCATTGAAGGTTTGGATCACATTTTAA
ATGGATTTGATGGTGAATTGATTCAGCCAGTTTTGAATAACTTTAAGAAAAATGGAGTCGAAATTGTTACTGAAGCTACA
GCGGTTGAGGCAAACCAAACTGACAAAGATGTTACAGTAACCTATGAAGCTGATGGAACAACCAAGACGGTTAACGGAGA
CTACTGTTTAGTTTCAGTAGGCCGCCGTCCTAACACAGATCAGCTTGGTTTGAATAATACCAATATCAAGTTAAGTAAGC
GTGGTTTAATTGAAGTTAATGATTCCATGCAAACATCAGTTAAACATATTTATGCTATCGGTGATGTGGTCGCTGGACCA
GCTTTAGCGCATAAGGCTAGTTTTGAGGCTAAGGTGGCAGCAGCTGCTATGGGTGGCGAAGATGCTCATGATACACATTA
TGTTTTGCCAGCCGTTGCTTACACAAACATTGAATTAGCAACTGTAGGTGAAACTCCGCAATCTATTGCTGAAAAGAAGT
TGGATGCTAAATCAAGCAAGTTCCCATTTGCTGCTAGTGGACGTGCCATGACTATGGATCAAACAGAAGGCTTTATCCGT
CTAATTACGGATAATCCTACTGGCGGTATTATTGGAGCGCAAATTGTGGGTCCGGAAGCTTCTAATTTGATTTCTGAGCT
AACTTTAGCGATTGAAAATGGATTAACAATTAAAGATATTGAATTAACCATTCATCCACATCCAACGTTGGGTGAAGAAA
TTATGGATGCAGCGGAATTAGCTGCAGGACTACCAATTCACGTTTAA

Upstream 100 bases:

>100_bases
TTGATCATCGTTTAATTGACGGTGCTCTAGCACAAAAAATTATGAATGAGCTGAAGGCACTTTTGAGTAACCCTGCCTAC
ATGCTAATGGAGGTGTAGTC

Downstream 100 bases:

>100_bases
AAAGAATTTGAAATATATTACAGTCATAAGATAAATAAACGGACCGATTCCCGATATGCCAGCTAAGGAATCGGTCTTTC
TGTTATCTTAAAGGTGTATA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MVVGTQAINVDTLIIGSGPGGYVAAIRAAELGQKVVIVERDYIGGVCLNVGCIPSKALIQAGHLYSTLQHGNPFGVSVSE
SKIDFTKTQEWKQNQVVNKLTGGVELLLKKHKVAIVRGEAYFNDNETVNVINGDDSHVYRFKRALIATGSRPVEIPHFKF
SGRVIDSTGALNLKDVPEHLIIIGGGVIGAELAGAYMNLGSKITIIEGLDHILNGFDGELIQPVLNNFKKNGVEIVTEAT
AVEANQTDKDVTVTYEADGTTKTVNGDYCLVSVGRRPNTDQLGLNNTNIKLSKRGLIEVNDSMQTSVKHIYAIGDVVAGP
ALAHKASFEAKVAAAAMGGEDAHDTHYVLPAVAYTNIELATVGETPQSIAEKKLDAKSSKFPFAASGRAMTMDQTEGFIR
LITDNPTGGIIGAQIVGPEASNLISELTLAIENGLTIKDIELTIHPHPTLGEEIMDAAELAAGLPIHV

Sequences:

>Translated_468_residues
MVVGTQAINVDTLIIGSGPGGYVAAIRAAELGQKVVIVERDYIGGVCLNVGCIPSKALIQAGHLYSTLQHGNPFGVSVSE
SKIDFTKTQEWKQNQVVNKLTGGVELLLKKHKVAIVRGEAYFNDNETVNVINGDDSHVYRFKRALIATGSRPVEIPHFKF
SGRVIDSTGALNLKDVPEHLIIIGGGVIGAELAGAYMNLGSKITIIEGLDHILNGFDGELIQPVLNNFKKNGVEIVTEAT
AVEANQTDKDVTVTYEADGTTKTVNGDYCLVSVGRRPNTDQLGLNNTNIKLSKRGLIEVNDSMQTSVKHIYAIGDVVAGP
ALAHKASFEAKVAAAAMGGEDAHDTHYVLPAVAYTNIELATVGETPQSIAEKKLDAKSSKFPFAASGRAMTMDQTEGFIR
LITDNPTGGIIGAQIVGPEASNLISELTLAIENGLTIKDIELTIHPHPTLGEEIMDAAELAAGLPIHV
>Mature_468_residues
MVVGTQAINVDTLIIGSGPGGYVAAIRAAELGQKVVIVERDYIGGVCLNVGCIPSKALIQAGHLYSTLQHGNPFGVSVSE
SKIDFTKTQEWKQNQVVNKLTGGVELLLKKHKVAIVRGEAYFNDNETVNVINGDDSHVYRFKRALIATGSRPVEIPHFKF
SGRVIDSTGALNLKDVPEHLIIIGGGVIGAELAGAYMNLGSKITIIEGLDHILNGFDGELIQPVLNNFKKNGVEIVTEAT
AVEANQTDKDVTVTYEADGTTKTVNGDYCLVSVGRRPNTDQLGLNNTNIKLSKRGLIEVNDSMQTSVKHIYAIGDVVAGP
ALAHKASFEAKVAAAAMGGEDAHDTHYVLPAVAYTNIELATVGETPQSIAEKKLDAKSSKFPFAASGRAMTMDQTEGFIR
LITDNPTGGIIGAQIVGPEASNLISELTLAIENGLTIKDIELTIHPHPTLGEEIMDAAELAAGLPIHV

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=40.2985074626866, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=29.2110874200426, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=28.4782608695652, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI33519430, Length=452, Percent_Identity=25.4424778761062, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519428, Length=452, Percent_Identity=25.4424778761062, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519426, Length=452, Percent_Identity=25.4424778761062, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI148277065, Length=452, Percent_Identity=25.4424778761062, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI148277071, Length=452, Percent_Identity=25.4424778761062, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI291045266, Length=460, Percent_Identity=26.7391304347826, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=26.3858093126386, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786307, Length=460, Percent_Identity=39.7826086956522, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=29.0948275862069, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=28.5714285714286, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1789915, Length=442, Percent_Identity=28.7330316742081, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=40.1315789473684, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI71983429, Length=444, Percent_Identity=27.2522522522523, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=444, Percent_Identity=27.2522522522523, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=27.4633123689727, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=476, Percent_Identity=23.3193277310924, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=38.2663847780127, Blast_Score=289, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=30.4439746300211, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=27.8131634819533, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=40.5579399141631, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24640553, Length=483, Percent_Identity=26.0869565217391, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17737741, Length=491, Percent_Identity=25.6619144602851, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=26.0869565217391, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=26.0869565217391, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49701; Mature: 49701

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGTQAINVDTLIIGSGPGGYVAAIRAAELGQKVVIVERDYIGGVCLNVGCIPSKALIQ
CEEECEEEEEEEEEEECCCCCEEEEEEHHHCCCEEEEEECCCCCEEEEEECCCCCHHHHH
AGHLYSTLQHGNPFGVSVSESKIDFTKTQEWKQNQVVNKLTGGVELLLKKHKVAIVRGEA
HHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHEECCEEEEEECCE
YFNDNETVNVINGDDSHVYRFKRALIATGSRPVEIPHFKFSGRVIDSTGALNLKDVPEHL
EECCCCEEEEECCCCHHHHHHHHHHHHCCCCCEECCCEEECCEEEECCCCCCCCCCCCEE
IIIGGGVIGAELAGAYMNLGSKITIIEGLDHILNGFDGELIQPVLNNFKKNGVEIVTEAT
EEEECCCHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEE
AVEANQTDKDVTVTYEADGTTKTVNGDYCLVSVGRRPNTDQLGLNNTNIKLSKRGLIEVN
EEECCCCCCCEEEEEECCCCEEEECCCEEEEECCCCCCCCCCCCCCCEEEEECCCEEEEC
DSMQTSVKHIYAIGDVVAGPALAHKASFEAKVAAAAMGGEDAHDTHYVLPAVAYTNIELA
CCHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEEEECEEEE
TVGETPQSIAEKKLDAKSSKFPFAASGRAMTMDQTEGFIRLITDNPTGGIIGAQIVGPEA
ECCCCHHHHHHHHCCCCCCCCCEECCCCEEEEECCCCEEEEEECCCCCCEEEEEEECCCH
SNLISELTLAIENGLTIKDIELTIHPHPTLGEEIMDAAELAAGLPIHV
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVVGTQAINVDTLIIGSGPGGYVAAIRAAELGQKVVIVERDYIGGVCLNVGCIPSKALIQ
CEEECEEEEEEEEEEECCCCCEEEEEEHHHCCCEEEEEECCCCCEEEEEECCCCCHHHHH
AGHLYSTLQHGNPFGVSVSESKIDFTKTQEWKQNQVVNKLTGGVELLLKKHKVAIVRGEA
HHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHEECCEEEEEECCE
YFNDNETVNVINGDDSHVYRFKRALIATGSRPVEIPHFKFSGRVIDSTGALNLKDVPEHL
EECCCCEEEEECCCCHHHHHHHHHHHHCCCCCEECCCEEECCEEEECCCCCCCCCCCCEE
IIIGGGVIGAELAGAYMNLGSKITIIEGLDHILNGFDGELIQPVLNNFKKNGVEIVTEAT
EEEECCCHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEE
AVEANQTDKDVTVTYEADGTTKTVNGDYCLVSVGRRPNTDQLGLNNTNIKLSKRGLIEVN
EEECCCCCCCEEEEEECCCCEEEECCCEEEEECCCCCCCCCCCCCCCEEEEECCCEEEEC
DSMQTSVKHIYAIGDVVAGPALAHKASFEAKVAAAAMGGEDAHDTHYVLPAVAYTNIELA
CCHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEEEECEEEE
TVGETPQSIAEKKLDAKSSKFPFAASGRAMTMDQTEGFIRLITDNPTGGIIGAQIVGPEA
ECCCCHHHHHHHHCCCCCCCCCEECCCCEEEEECCCCEEEEEECCCCCCEEEEEEECCCH
SNLISELTLAIENGLTIKDIELTIHPHPTLGEEIMDAAELAAGLPIHV
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]