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The map label for this gene is glpF [H]

Identifier: 116493419

GI number: 116493419

Start: 1666412

End: 1667125

Strand: Reverse

Name: glpF [H]

Synonym: PEPE_1691

Alternate gene names: 116493419

Gene position: 1667125-1666412 (Counterclockwise)

Preceding gene: 116493420

Following gene: 116493418

Centisome position: 90.98

GC content: 37.68

Gene sequence:

>714_bases
ATGGTAAATCATCTATTAGCCGAATTTATGGGAACAGCCCTTATGATAATTTTTGGTGTGGGAGTTCATTGTGACGAAGT
TTTAAAGAAGACCAAATATGCCGGTTCAGGTCACTTATTTGCAATTACAACTTGGGCATTTGGTATTTCAGTTGTGTTAT
TTATTTTTGGTGGCGTATGTATTAACCCTGCTATGGCACTTACTCAAGCAATTTTAGGTATGATTCCTTGGAGTTACTTT
ATTCCTTATACAATCGCCGAAGTATTGGGTGGTGTAGTTGGGGCAGTGATCGTTTACATTATGTATGCTGATCACTTTAA
AGAATCAGAAGGTTCAATTGATCCGATCGCAATTAGAAATATTTTCTCAACTAATCCTAACTTACGTAATTTACCACGTA
ACTATTTTGTTGAAACATTCGCTACATTCGTTTTCCTAACATCAATTCTAGCAATCGTTCATGGTTACGATTCAATGTCA
CCAATAGCCGTGGGACTCTTAGTATGGGCCATTGGTATGGGACTTGGAGGTACGACAGGATTCGCGATGAACCAAGCTCG
TGACTTGGGACCGCGCTTAGCATTCCAATTACTTCCTATTAAAAATAAAGCTAATAATGATTGGCAATATGGTCTAATTG
TTCCAGGAACTGCACCATTTGTTGGTTCAATTTTGGCAGCAGCTTTTGTTAGATTTTACTTACACATTTACTAA

Upstream 100 bases:

>100_bases
GACTGCGAAAAAATAGCAACTAAAAATGATTGGTCATTACAAGCAACATATAAAAAAATACAGAATTTATATTAAGTAGA
TAACAAAGGAGTTTAATTTA

Downstream 100 bases:

>100_bases
AAAAATCGATAATTAAAGGAGAAACATTATTATGACTAACTTAGAAGCAAAAGAAAATAATTTAATTAAGGATTTAGCAT
CAAAGCAAAGAGTTGCAATC

Product: glycerol uptake facilitator related permease (major Intrinsic protein family)

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MVNHLLAEFMGTALMIIFGVGVHCDEVLKKTKYAGSGHLFAITTWAFGISVVLFIFGGVCINPAMALTQAILGMIPWSYF
IPYTIAEVLGGVVGAVIVYIMYADHFKESEGSIDPIAIRNIFSTNPNLRNLPRNYFVETFATFVFLTSILAIVHGYDSMS
PIAVGLLVWAIGMGLGGTTGFAMNQARDLGPRLAFQLLPIKNKANNDWQYGLIVPGTAPFVGSILAAAFVRFYLHIY

Sequences:

>Translated_237_residues
MVNHLLAEFMGTALMIIFGVGVHCDEVLKKTKYAGSGHLFAITTWAFGISVVLFIFGGVCINPAMALTQAILGMIPWSYF
IPYTIAEVLGGVVGAVIVYIMYADHFKESEGSIDPIAIRNIFSTNPNLRNLPRNYFVETFATFVFLTSILAIVHGYDSMS
PIAVGLLVWAIGMGLGGTTGFAMNQARDLGPRLAFQLLPIKNKANNDWQYGLIVPGTAPFVGSILAAAFVRFYLHIY
>Mature_237_residues
MVNHLLAEFMGTALMIIFGVGVHCDEVLKKTKYAGSGHLFAITTWAFGISVVLFIFGGVCINPAMALTQAILGMIPWSYF
IPYTIAEVLGGVVGAVIVYIMYADHFKESEGSIDPIAIRNIFSTNPNLRNLPRNYFVETFATFVFLTSILAIVHGYDSMS
PIAVGLLVWAIGMGLGGTTGFAMNQARDLGPRLAFQLLPIKNKANNDWQYGLIVPGTAPFVGSILAAAFVRFYLHIY

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=245, Percent_Identity=32.2448979591837, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI157266307, Length=243, Percent_Identity=29.2181069958848, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI4502187, Length=248, Percent_Identity=28.6290322580645, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI22538420, Length=254, Percent_Identity=25.1968503937008, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1790362, Length=246, Percent_Identity=31.3008130081301, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531431, Length=237, Percent_Identity=32.4894514767932, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531429, Length=237, Percent_Identity=32.4894514767932, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994009, Length=246, Percent_Identity=25.609756097561, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71992966, Length=253, Percent_Identity=24.901185770751, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=198, Percent_Identity=31.8181818181818, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25823; Mature: 25823

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNHLLAEFMGTALMIIFGVGVHCDEVLKKTKYAGSGHLFAITTWAFGISVVLFIFGGVC
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
INPAMALTQAILGMIPWSYFIPYTIAEVLGGVVGAVIVYIMYADHFKESEGSIDPIAIRN
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IFSTNPNLRNLPRNYFVETFATFVFLTSILAIVHGYDSMSPIAVGLLVWAIGMGLGGTTG
HHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCH
FAMNQARDLGPRLAFQLLPIKNKANNDWQYGLIVPGTAPFVGSILAAAFVRFYLHIY
HHHHHHHHCCHHHHHHEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVNHLLAEFMGTALMIIFGVGVHCDEVLKKTKYAGSGHLFAITTWAFGISVVLFIFGGVC
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
INPAMALTQAILGMIPWSYFIPYTIAEVLGGVVGAVIVYIMYADHFKESEGSIDPIAIRN
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IFSTNPNLRNLPRNYFVETFATFVFLTSILAIVHGYDSMSPIAVGLLVWAIGMGLGGTTG
HHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCH
FAMNQARDLGPRLAFQLLPIKNKANNDWQYGLIVPGTAPFVGSILAAAFVRFYLHIY
HHHHHHHHCCHHHHHHEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]