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The map label for this gene is pyrF [H]

Identifier: 116493403

GI number: 116493403

Start: 1655713

End: 1656453

Strand: Reverse

Name: pyrF [H]

Synonym: PEPE_1675

Alternate gene names: 116493403

Gene position: 1656453-1655713 (Counterclockwise)

Preceding gene: 116493405

Following gene: 116493401

Centisome position: 90.4

GC content: 41.3

Gene sequence:

>741_bases
ATGTTAAGAGCGGTTCAGAATGGAGTTTTTATGGAAAAACCAGTTATGATTGCGCTAGATTTTAAGGATCGGGGAGAACT
TAACCAATTCTTGGAGCAATTTCCGGAACATTTGAAGTTAACCATTAAATTGGGAATGGAACTCTTCTACGGAATGGGTC
CCGAAATCGTTCGAGATATGCGTCAAAAAGGGCATGACATCTTTCTTGATTTAAAATTGAACGATATTCCTAATACGGTA
GAAAAAGCAATGATGCAATTAGGTCGAATGGGGGTTCAATACACAACAATCCATGCGATGGGTGGTTCTGATATGATTCG
AGCGGCTAAACGAGGATTGGTTGCTGGAGCTAGAGAAGTAGACTTACCAACGCCTAAACTATTAGCAGTAACGGAATTAA
CTTCTATTTCAGAAAAGCAACTAAGTGAAGAACAGAATGTTAGTTTACCAATGGCAGATCAAGTACTTAGCCTAGCAAAA
TTGACTAGTACGGCTGGCGGAGATGGTGTAATTTGTTCTCCAAACGAAGTTAGTTATTTAAAACCCCAACTTCCAGAAGA
TTTTCTGTTTGTGACACCTGGAATTCGACCTGCCGGAGCAGCAAAGGGTGATCAAAAACGGACGATGACTGCTGCGGAAG
CTGCCAAAGCAGGAAGCTCAGCTATTGTGGTAGGTAGACCAATTACTCAATCTGAGGACCCGGTTGTGGCGTACCAAGCA
ATTTTAAGTGAATTTAACTAA

Upstream 100 bases:

>100_bases
AATCGAGTCGCTTTAAATCGATCCAGAGAGATTGATAAGCTAACGAATACACCGCAAACTAGGTGCTGGTTAGCATACCT
AGACCTTGCGGTGTTTTTTG

Downstream 100 bases:

>100_bases
AAAAATCGGCGTTGTCAAAACTTTTGAAATTAAGCTCAAAAGTTAATTGACCGCCGATTTTTTATAATAGTTTTTCTAGG
GTGTCTAATACACCATCATG

Product: orotidine-5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MLRAVQNGVFMEKPVMIALDFKDRGELNQFLEQFPEHLKLTIKLGMELFYGMGPEIVRDMRQKGHDIFLDLKLNDIPNTV
EKAMMQLGRMGVQYTTIHAMGGSDMIRAAKRGLVAGAREVDLPTPKLLAVTELTSISEKQLSEEQNVSLPMADQVLSLAK
LTSTAGGDGVICSPNEVSYLKPQLPEDFLFVTPGIRPAGAAKGDQKRTMTAAEAAKAGSSAIVVGRPITQSEDPVVAYQA
ILSEFN

Sequences:

>Translated_246_residues
MLRAVQNGVFMEKPVMIALDFKDRGELNQFLEQFPEHLKLTIKLGMELFYGMGPEIVRDMRQKGHDIFLDLKLNDIPNTV
EKAMMQLGRMGVQYTTIHAMGGSDMIRAAKRGLVAGAREVDLPTPKLLAVTELTSISEKQLSEEQNVSLPMADQVLSLAK
LTSTAGGDGVICSPNEVSYLKPQLPEDFLFVTPGIRPAGAAKGDQKRTMTAAEAAKAGSSAIVVGRPITQSEDPVVAYQA
ILSEFN
>Mature_246_residues
MLRAVQNGVFMEKPVMIALDFKDRGELNQFLEQFPEHLKLTIKLGMELFYGMGPEIVRDMRQKGHDIFLDLKLNDIPNTV
EKAMMQLGRMGVQYTTIHAMGGSDMIRAAKRGLVAGAREVDLPTPKLLAVTELTSISEKQLSEEQNVSLPMADQVLSLAK
LTSTAGGDGVICSPNEVSYLKPQLPEDFLFVTPGIRPAGAAKGDQKRTMTAAEAAKAGSSAIVVGRPITQSEDPVVAYQA
ILSEFN

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=240, Percent_Identity=41.6666666666667, Blast_Score=171, Evalue=4e-44,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 26791; Mature: 26791

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRAVQNGVFMEKPVMIALDFKDRGELNQFLEQFPEHLKLTIKLGMELFYGMGPEIVRDM
CCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHEEHHHHHHHHHHCCCHHHHHHH
RQKGHDIFLDLKLNDIPNTVEKAMMQLGRMGVQYTTIHAMGGSDMIRAAKRGLVAGAREV
HHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCC
DLPTPKLLAVTELTSISEKQLSEEQNVSLPMADQVLSLAKLTSTAGGDGVICSPNEVSYL
CCCCCCEEEHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
KPQLPEDFLFVTPGIRPAGAAKGDQKRTMTAAEAAKAGSSAIVVGRPITQSEDPVVAYQA
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
ILSEFN
HHHHCC
>Mature Secondary Structure
MLRAVQNGVFMEKPVMIALDFKDRGELNQFLEQFPEHLKLTIKLGMELFYGMGPEIVRDM
CCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHEEHHHHHHHHHHCCCHHHHHHH
RQKGHDIFLDLKLNDIPNTVEKAMMQLGRMGVQYTTIHAMGGSDMIRAAKRGLVAGAREV
HHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCC
DLPTPKLLAVTELTSISEKQLSEEQNVSLPMADQVLSLAKLTSTAGGDGVICSPNEVSYL
CCCCCCEEEHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
KPQLPEDFLFVTPGIRPAGAAKGDQKRTMTAAEAAKAGSSAIVVGRPITQSEDPVVAYQA
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
ILSEFN
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA