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The map label for this gene is clpB [H]

Identifier: 116492858

GI number: 116492858

Start: 1093137

End: 1095728

Strand: Reverse

Name: clpB [H]

Synonym: PEPE_1096

Alternate gene names: 116492858

Gene position: 1095728-1093137 (Counterclockwise)

Preceding gene: 116492859

Following gene: 116492856

Centisome position: 59.8

GC content: 37.31

Gene sequence:

>2592_bases
ATGAATCCAGAGAAAATGACTACGGCACTTCAAGAAGCAATTAGCGAAGCACAGCAGATTGCTGTAAATCGTCAACATCA
AGAAATTGGTGTGCCAGAATTATTTAAATTTTTAACACAACCAGACCAACTGGTCGGGACACTATTAACAGACTTAAAGA
TTGATAACAACCTTGTCCAAACCGAGTTAGACCAAGAACTTGAACAAATTAGCGTGGTTAGTGGTAGCCAGGTTAGTTAC
GGTCAATCGATTTCAGCAGAACTAAATCAATTATTAGTTCAAGCAGAACAACAGCGTAAAAAATTACAGGATGAGTATGT
TGCAATTGATACTATTTTAATTGCTTTATTTAATTTAGATCAGAATCAGTTTAAAAAATTCCTGAATCAACAAGGTCTAA
CTCAAAAACAGGTTTTAGCCAAAGTAGATGAATTTCGTGGGGGTGAAAGAGTGACATCAAAGGAACAAGAAAGTGGATAT
AAAGCGCTAGAAAAATATGGGACAGATTTAGTTAAAAGGGCCCACAGTGAAAACCCAGATCCAGTTATTGGACGTGATGA
AGAAATTTTAGAAGTTATTCGAATTTTATCGCGAAAAACAAAAAATAATCCAGTACTAATTGGTGAACCTGGTGTAGGAA
AAACAGCCATTGTAGAAGGTCTAGCGCAAAGAATTGCTAAAGGTGATGTTCCTAGCAATTTAGTTGATAAAACCATTGTT
TCTCTAGATATGGGCTCATTAATTGCCGGGGCTAAATATCGAGGCGATTTTGAAGAACGACTTAAAGCAGTTTTGAAGGA
AGTATCAAAGTCAGATGGTCAAATTATCTTATTCATTGATGAAATTCACACGATTGTAGGAGCGGGTAAGACTGAAGGTA
GTATGGATGCTGGAAATCTTTTGAAACCTATGCTAGCTCGTGGTGAACTCCATTTGATTGGTGCAACAACATTAGATGAA
TACCGCGAAAACATTGAAAAAGACAAGGCACTTGAGCGCCGTTTCCAACGGGTGCTGGTTAAGGAGCCTAGCATTGAGGA
TACTATCAGTATTTTAAGAGGTTTAAAGGAACGCTTTGAAATTCATCATGGCGTTCGTATCCATGATAATGCTCTTGTGG
CAGCCGCTAAACTTTCTAGTCGTTATATTACGGATCGTTATTTACCAGATAAGGCGATTGATTTAATCGATGAAGCATCT
GCAAAAATTAAAGTGGAAATGAATTCCAATCCTACTGAACTCGATCAGGTCAAACGACAGTTGATGCGTTTGCAGGTTGA
AGAACGAGCACTTAAAAATGAAAGTGATGCAGAGTCAGTTAAACGTTTAAAGGACTTACAGAAAGAATTATCTGAAACCA
AAGAAGAAAAAGTTAAGCTGGAATCACGTTGGTCAGCCCAAAAAGAAGAAATTAAAAAGATCAGTGATAAGAAGAATGAA
TTAGATAAGGCCAAGCATGACTTACAAGAAGCTGAAAGTTCTTATGATCTCGATCAAGCGGCTATTTTACAACATGGGAC
CATTCCAGCTTTGGAAAAAGAATTGGAACAATTAGAAAATCAATCCGAAAGTGATCATGGGGATTGGTTGGTAGAAGAAT
CCGTTACAGATAAAGAAATTGCCCAAACTATTAGTCAAATGACAGGCATTCCAGTTGCACGGTTGGTGGAAGGTGAACGA
GAAAAACTGCTACATTTAGCTGATAAATTACACAATCGAGTAATTGGTCAAGATGAAGCCGTTCAATCCGTTTCAGATGC
GGTTTTACGTTCCCGTGCGGGACTCCAGGATCCTAATCGTCCGATTGGTTCATTTCTCTTCCTTGGTCCGACAGGGGTTG
GTAAGACAGAATTGTCTAAAGCTTTAGCAGAAGCGTTATTTGATTCGGAAGATAATATGGTCCGAATCGATATGTCTGAA
TACATGGAAAAAGAATCGGTTTCCCGTTTGGTTGGAGCTGCTCCAGGATATATTGGTTACGAAGAGGGAGGCCAATTAAC
CGAAGCGGTCCGGCGAAACCCATATTCCATTGTTCTATTTGATGAAATTGAAAAAGCACATCCAGAAGTGTTCAATATTC
TTTTACAAGTTCTAGATGATGGTCGTTTAACAGATGGACAAGGACGTACGGTTAACTTTAGAAATACAATTTTAATTATG
ACTTCTAACTTAGGTTCAGATTTATTATTGAAGAATCAATCAGAAAACAAGATTGATGATAATATCCGTAACCAAGTTCA
AGATGTTTTGCATGGATACTTTAGACCAGAATTTTTAAATCGGATTGATGATATCATTATGTTTACTCCATTAAGTAAAC
AAGATGTACGCCAAATTGTGATTAAATTAATTCATAATTTATCTATGCGTTTGAGCGACCAAGAAATAAGTTTAAGTATT
TCGGATGATGCACAAGATTATATTGTTGAACAGGCATATGATCCAGCGTTTGGTGCTCGACCATTGCGCCGCTACATTAC
TAAAAATGTTGAAACTCCTTTGGCAAAAGAAATTATTAGTGGGCGTGTCCGTCCTAATTCAACAGTCAATATCGATGTTT
TGAATGATCAATTGGTTTTCCAGAATAAATAA

Upstream 100 bases:

>100_bases
AACGAATGGAAGGCTAATAATGATTGGGAAGTTGAAATTATAGAATCGACGGTTAATACCAATCCTTATCAATTTATCTA
AAAATTGGGAGGCGATTTAA

Downstream 100 bases:

>100_bases
AAAAAGGATGCTCTCTTTGGTAAAAATACCAAAGATTTGCATCCTTATTTTTTAGGTTTTGAAATTAGTGGGATCATGTT
AGAAGTGATAAGAGCTACTA

Product: ATP-binding subunit of Clp protease and DnaK/DnaJ chaperones

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MNPEKMTTALQEAISEAQQIAVNRQHQEIGVPELFKFLTQPDQLVGTLLTDLKIDNNLVQTELDQELEQISVVSGSQVSY
GQSISAELNQLLVQAEQQRKKLQDEYVAIDTILIALFNLDQNQFKKFLNQQGLTQKQVLAKVDEFRGGERVTSKEQESGY
KALEKYGTDLVKRAHSENPDPVIGRDEEILEVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIAKGDVPSNLVDKTIV
SLDMGSLIAGAKYRGDFEERLKAVLKEVSKSDGQIILFIDEIHTIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDE
YRENIEKDKALERRFQRVLVKEPSIEDTISILRGLKERFEIHHGVRIHDNALVAAAKLSSRYITDRYLPDKAIDLIDEAS
AKIKVEMNSNPTELDQVKRQLMRLQVEERALKNESDAESVKRLKDLQKELSETKEEKVKLESRWSAQKEEIKKISDKKNE
LDKAKHDLQEAESSYDLDQAAILQHGTIPALEKELEQLENQSESDHGDWLVEESVTDKEIAQTISQMTGIPVARLVEGER
EKLLHLADKLHNRVIGQDEAVQSVSDAVLRSRAGLQDPNRPIGSFLFLGPTGVGKTELSKALAEALFDSEDNMVRIDMSE
YMEKESVSRLVGAAPGYIGYEEGGQLTEAVRRNPYSIVLFDEIEKAHPEVFNILLQVLDDGRLTDGQGRTVNFRNTILIM
TSNLGSDLLLKNQSENKIDDNIRNQVQDVLHGYFRPEFLNRIDDIIMFTPLSKQDVRQIVIKLIHNLSMRLSDQEISLSI
SDDAQDYIVEQAYDPAFGARPLRRYITKNVETPLAKEIISGRVRPNSTVNIDVLNDQLVFQNK

Sequences:

>Translated_863_residues
MNPEKMTTALQEAISEAQQIAVNRQHQEIGVPELFKFLTQPDQLVGTLLTDLKIDNNLVQTELDQELEQISVVSGSQVSY
GQSISAELNQLLVQAEQQRKKLQDEYVAIDTILIALFNLDQNQFKKFLNQQGLTQKQVLAKVDEFRGGERVTSKEQESGY
KALEKYGTDLVKRAHSENPDPVIGRDEEILEVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIAKGDVPSNLVDKTIV
SLDMGSLIAGAKYRGDFEERLKAVLKEVSKSDGQIILFIDEIHTIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDE
YRENIEKDKALERRFQRVLVKEPSIEDTISILRGLKERFEIHHGVRIHDNALVAAAKLSSRYITDRYLPDKAIDLIDEAS
AKIKVEMNSNPTELDQVKRQLMRLQVEERALKNESDAESVKRLKDLQKELSETKEEKVKLESRWSAQKEEIKKISDKKNE
LDKAKHDLQEAESSYDLDQAAILQHGTIPALEKELEQLENQSESDHGDWLVEESVTDKEIAQTISQMTGIPVARLVEGER
EKLLHLADKLHNRVIGQDEAVQSVSDAVLRSRAGLQDPNRPIGSFLFLGPTGVGKTELSKALAEALFDSEDNMVRIDMSE
YMEKESVSRLVGAAPGYIGYEEGGQLTEAVRRNPYSIVLFDEIEKAHPEVFNILLQVLDDGRLTDGQGRTVNFRNTILIM
TSNLGSDLLLKNQSENKIDDNIRNQVQDVLHGYFRPEFLNRIDDIIMFTPLSKQDVRQIVIKLIHNLSMRLSDQEISLSI
SDDAQDYIVEQAYDPAFGARPLRRYITKNVETPLAKEIISGRVRPNSTVNIDVLNDQLVFQNK
>Mature_863_residues
MNPEKMTTALQEAISEAQQIAVNRQHQEIGVPELFKFLTQPDQLVGTLLTDLKIDNNLVQTELDQELEQISVVSGSQVSY
GQSISAELNQLLVQAEQQRKKLQDEYVAIDTILIALFNLDQNQFKKFLNQQGLTQKQVLAKVDEFRGGERVTSKEQESGY
KALEKYGTDLVKRAHSENPDPVIGRDEEILEVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIAKGDVPSNLVDKTIV
SLDMGSLIAGAKYRGDFEERLKAVLKEVSKSDGQIILFIDEIHTIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDE
YRENIEKDKALERRFQRVLVKEPSIEDTISILRGLKERFEIHHGVRIHDNALVAAAKLSSRYITDRYLPDKAIDLIDEAS
AKIKVEMNSNPTELDQVKRQLMRLQVEERALKNESDAESVKRLKDLQKELSETKEEKVKLESRWSAQKEEIKKISDKKNE
LDKAKHDLQEAESSYDLDQAAILQHGTIPALEKELEQLENQSESDHGDWLVEESVTDKEIAQTISQMTGIPVARLVEGER
EKLLHLADKLHNRVIGQDEAVQSVSDAVLRSRAGLQDPNRPIGSFLFLGPTGVGKTELSKALAEALFDSEDNMVRIDMSE
YMEKESVSRLVGAAPGYIGYEEGGQLTEAVRRNPYSIVLFDEIEKAHPEVFNILLQVLDDGRLTDGQGRTVNFRNTILIM
TSNLGSDLLLKNQSENKIDDNIRNQVQDVLHGYFRPEFLNRIDDIIMFTPLSKQDVRQIVIKLIHNLSMRLSDQEISLSI
SDDAQDYIVEQAYDPAFGARPLRRYITKNVETPLAKEIISGRVRPNSTVNIDVLNDQLVFQNK

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=299, Percent_Identity=35.7859531772575, Blast_Score=201, Evalue=3e-51,
Organism=Escherichia coli, GI1788943, Length=866, Percent_Identity=52.1939953810624, Blast_Score=871, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=310, Percent_Identity=47.741935483871, Blast_Score=283, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=707, Percent_Identity=51.9094766619519, Blast_Score=741, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=850, Percent_Identity=41.5294117647059, Blast_Score=645, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 97125; Mature: 97125

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPEKMTTALQEAISEAQQIAVNRQHQEIGVPELFKFLTQPDQLVGTLLTDLKIDNNLVQ
CCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHH
TELDQELEQISVVSGSQVSYGQSISAELNQLLVQAEQQRKKLQDEYVAIDTILIALFNLD
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QNQFKKFLNQQGLTQKQVLAKVDEFRGGERVTSKEQESGYKALEKYGTDLVKRAHSENPD
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVIGRDEEILEVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIAKGDVPSNLVDKTIV
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
SLDMGSLIAGAKYRGDFEERLKAVLKEVSKSDGQIILFIDEIHTIVGAGKTEGSMDAGNL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCHHHH
LKPMLARGELHLIGATTLDEYRENIEKDKALERRFQRVLVKEPSIEDTISILRGLKERFE
HHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IHHGVRIHDNALVAAAKLSSRYITDRYLPDKAIDLIDEASAKIKVEMNSNPTELDQVKRQ
HHCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LMRLQVEERALKNESDAESVKRLKDLQKELSETKEEKVKLESRWSAQKEEIKKISDKKNE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LDKAKHDLQEAESSYDLDQAAILQHGTIPALEKELEQLENQSESDHGDWLVEESVTDKEI
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
AQTISQMTGIPVARLVEGEREKLLHLADKLHNRVIGQDEAVQSVSDAVLRSRAGLQDPNR
HHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
PIGSFLFLGPTGVGKTELSKALAEALFDSEDNMVRIDMSEYMEKESVSRLVGAAPGYIGY
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCC
EEGGQLTEAVRRNPYSIVLFDEIEKAHPEVFNILLQVLDDGRLTDGQGRTVNFRNTILIM
CCCCHHHHHHHCCCCEEEEEHHHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEE
TSNLGSDLLLKNQSENKIDDNIRNQVQDVLHGYFRPEFLNRIDDIIMFTPLSKQDVRQIV
ECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHH
IKLIHNLSMRLSDQEISLSISDDAQDYIVEQAYDPAFGARPLRRYITKNVETPLAKEIIS
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHC
GRVRPNSTVNIDVLNDQLVFQNK
CCCCCCCEEEEEEECCCEEECCC
>Mature Secondary Structure
MNPEKMTTALQEAISEAQQIAVNRQHQEIGVPELFKFLTQPDQLVGTLLTDLKIDNNLVQ
CCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHH
TELDQELEQISVVSGSQVSYGQSISAELNQLLVQAEQQRKKLQDEYVAIDTILIALFNLD
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QNQFKKFLNQQGLTQKQVLAKVDEFRGGERVTSKEQESGYKALEKYGTDLVKRAHSENPD
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVIGRDEEILEVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIAKGDVPSNLVDKTIV
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
SLDMGSLIAGAKYRGDFEERLKAVLKEVSKSDGQIILFIDEIHTIVGAGKTEGSMDAGNL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCHHHH
LKPMLARGELHLIGATTLDEYRENIEKDKALERRFQRVLVKEPSIEDTISILRGLKERFE
HHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IHHGVRIHDNALVAAAKLSSRYITDRYLPDKAIDLIDEASAKIKVEMNSNPTELDQVKRQ
HHCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LMRLQVEERALKNESDAESVKRLKDLQKELSETKEEKVKLESRWSAQKEEIKKISDKKNE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LDKAKHDLQEAESSYDLDQAAILQHGTIPALEKELEQLENQSESDHGDWLVEESVTDKEI
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
AQTISQMTGIPVARLVEGEREKLLHLADKLHNRVIGQDEAVQSVSDAVLRSRAGLQDPNR
HHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
PIGSFLFLGPTGVGKTELSKALAEALFDSEDNMVRIDMSEYMEKESVSRLVGAAPGYIGY
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCC
EEGGQLTEAVRRNPYSIVLFDEIEKAHPEVFNILLQVLDDGRLTDGQGRTVNFRNTILIM
CCCCHHHHHHHCCCCEEEEEHHHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEE
TSNLGSDLLLKNQSENKIDDNIRNQVQDVLHGYFRPEFLNRIDDIIMFTPLSKQDVRQIV
ECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHH
IKLIHNLSMRLSDQEISLSISDDAQDYIVEQAYDPAFGARPLRRYITKNVETPLAKEIIS
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHC
GRVRPNSTVNIDVLNDQLVFQNK
CCCCCCCEEEEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]