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The map label for this gene is thyA

Identifier: 116492833

GI number: 116492833

Start: 1066273

End: 1067223

Strand: Reverse

Name: thyA

Synonym: PEPE_1070

Alternate gene names: 116492833

Gene position: 1067223-1066273 (Counterclockwise)

Preceding gene: 116492834

Following gene: 116492832

Centisome position: 58.24

GC content: 37.12

Gene sequence:

>951_bases
ATGCTTGAAGAACAATACCTTAATTTGGAAAGATATGTTTTGGAAAATGGGCATCTCAAAGGAGACCGTACCCAAACTGG
TACCCTAAGTACTTTTGGATATCAAATGCGTTTTGATTTAAGTGAAGGTTTTCCATTACTAACGACCAAGAGAGTCCCTT
TTGGTTTGATTAAGAGTGAGTTACTCTGGTTTTTAAAAGGCGATACCAATATTAGATATTTACTTCAACATAATAATCAT
ATTTGGGATGAATGGGCTTTTAAAAAGTGGGTTGAAAGTGATGAATATCAGGGACCAGACATGACCGATTTTGGTCACCG
CTCACTGACTGATCCTGAATTCAATGAACTCTATAAAATTGAAAAACAACGTTTTACAGAACAAATTTTGGAAGATGATA
CATTTTCTGCTAAATATGGTGATCTTGGAAATGTTTACGGTAGCCAATGGCGAGCATGGAAGACTAGTACTGGTGAGACT
ATCGATCAAATTTCAAATGTTATTGATATGATTAAGAATAATCCTAACTCAAGAAGAATGATCGTTTCGGCTTGGAATCC
TGAAGATGTGCCAACCTCAGCATTACCTCCGTGTCATTCACTATTTCAATTTTATGTGGCTGATGGGAAACTTAGCTGTC
AACTGTATCAACGCAGTGGCGATATTTTCTTAGGAATTCCTTTCAATATTGCTAGCTATGCCTTGCTTACAGAATTAATT
GCGAAAGCGACTGGTTTAGAAGTGGGAGAATTTATCCATACCATCGGTGATGCTCACATTTATTCTAATCATCTGGATCA
GGTTAAAGAACAATTAGAACGTACTCCACGTCCGGCTCCTAAATTGAAATTTAAACAAGTGCATGACAGTATCTTTGACT
ATGAACCAGGAGATATTGTGGTTGAAGGATATGATCCTCATCCAACCATTAAAGCACCGGTAGCAGTTTAA

Upstream 100 bases:

>100_bases
AGAATTGCAAAAAGAATTAAATGAAATTTCGGACCAATTAGAAGAAAAAATGGCAAGATGGGAATACTTAGAAAGTTTCG
AAGAATAGGGGAAATTTAAT

Downstream 100 bases:

>100_bases
TTTTAAGTCAATTTAGGAGGAATTAAATTTGATTTCAATGATTTGGGCCGAAGATTTGCGTCATGGAATTGGCAAGCAGC
AATCTATTCCGTGGCATATT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLEEQYLNLERYVLENGHLKGDRTQTGTLSTFGYQMRFDLSEGFPLLTTKRVPFGLIKSELLWFLKGDTNIRYLLQHNNH
IWDEWAFKKWVESDEYQGPDMTDFGHRSLTDPEFNELYKIEKQRFTEQILEDDTFSAKYGDLGNVYGSQWRAWKTSTGET
IDQISNVIDMIKNNPNSRRMIVSAWNPEDVPTSALPPCHSLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYALLTELI
AKATGLEVGEFIHTIGDAHIYSNHLDQVKEQLERTPRPAPKLKFKQVHDSIFDYEPGDIVVEGYDPHPTIKAPVAV

Sequences:

>Translated_316_residues
MLEEQYLNLERYVLENGHLKGDRTQTGTLSTFGYQMRFDLSEGFPLLTTKRVPFGLIKSELLWFLKGDTNIRYLLQHNNH
IWDEWAFKKWVESDEYQGPDMTDFGHRSLTDPEFNELYKIEKQRFTEQILEDDTFSAKYGDLGNVYGSQWRAWKTSTGET
IDQISNVIDMIKNNPNSRRMIVSAWNPEDVPTSALPPCHSLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYALLTELI
AKATGLEVGEFIHTIGDAHIYSNHLDQVKEQLERTPRPAPKLKFKQVHDSIFDYEPGDIVVEGYDPHPTIKAPVAV
>Mature_316_residues
MLEEQYLNLERYVLENGHLKGDRTQTGTLSTFGYQMRFDLSEGFPLLTTKRVPFGLIKSELLWFLKGDTNIRYLLQHNNH
IWDEWAFKKWVESDEYQGPDMTDFGHRSLTDPEFNELYKIEKQRFTEQILEDDTFSAKYGDLGNVYGSQWRAWKTSTGET
IDQISNVIDMIKNNPNSRRMIVSAWNPEDVPTSALPPCHSLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYALLTELI
AKATGLEVGEFIHTIGDAHIYSNHLDQVKEQLERTPRPAPKLKFKQVHDSIFDYEPGDIVVEGYDPHPTIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=322, Percent_Identity=46.2732919254658, Blast_Score=281, Evalue=4e-76,
Organism=Escherichia coli, GI1789191, Length=313, Percent_Identity=52.3961661341853, Blast_Score=317, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI71993377, Length=323, Percent_Identity=42.4148606811146, Blast_Score=247, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6324648, Length=196, Percent_Identity=53.5714285714286, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI17137556, Length=324, Percent_Identity=46.9135802469136, Blast_Score=291, Evalue=6e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_PEDPA (Q03F96)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804568.1
- ProteinModelPortal:   Q03F96
- SMR:   Q03F96
- STRING:   Q03F96
- GeneID:   4417074
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1070
- NMPDR:   fig|278197.10.peg.936
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   KIWNEWA
- PhylomeDB:   Q03F96
- ProtClustDB:   PRK01827
- BioCyc:   PPEN278197:PEPE_1070-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 36392; Mature: 36392

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEEQYLNLERYVLENGHLKGDRTQTGTLSTFGYQMRFDLSEGFPLLTTKRVPFGLIKSE
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEEEEECCCCCCEEEECCCCHHHHHHH
LLWFLKGDTNIRYLLQHNNHIWDEWAFKKWVESDEYQGPDMTDFGHRSLTDPEFNELYKI
HEEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
EKQRFTEQILEDDTFSAKYGDLGNVYGSQWRAWKTSTGETIDQISNVIDMIKNNPNSRRM
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
IVSAWNPEDVPTSALPPCHSLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYALLTELI
EEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHH
AKATGLEVGEFIHTIGDAHIYSNHLDQVKEQLERTPRPAPKLKFKQVHDSIFDYEPGDIV
HHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEE
VEGYDPHPTIKAPVAV
EECCCCCCCCCCCCCC
>Mature Secondary Structure
MLEEQYLNLERYVLENGHLKGDRTQTGTLSTFGYQMRFDLSEGFPLLTTKRVPFGLIKSE
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEEEEECCCCCCEEEECCCCHHHHHHH
LLWFLKGDTNIRYLLQHNNHIWDEWAFKKWVESDEYQGPDMTDFGHRSLTDPEFNELYKI
HEEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
EKQRFTEQILEDDTFSAKYGDLGNVYGSQWRAWKTSTGETIDQISNVIDMIKNNPNSRRM
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
IVSAWNPEDVPTSALPPCHSLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYALLTELI
EEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHH
AKATGLEVGEFIHTIGDAHIYSNHLDQVKEQLERTPRPAPKLKFKQVHDSIFDYEPGDIV
HHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEE
VEGYDPHPTIKAPVAV
EECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA