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The map label for this gene is dfrA [H]

Identifier: 116492832

GI number: 116492832

Start: 1065759

End: 1066244

Strand: Reverse

Name: dfrA [H]

Synonym: PEPE_1069

Alternate gene names: 116492832

Gene position: 1066244-1065759 (Counterclockwise)

Preceding gene: 116492833

Following gene: 116492831

Centisome position: 58.19

GC content: 36.01

Gene sequence:

>486_bases
TTGATTTCAATGATTTGGGCCGAAGATTTGCGTCATGGAATTGGCAAGCAGCAATCTATTCCGTGGCATATTCCAGGTGA
TATGGCTTTTTTTAAGAAAACCACTAGTGGGAACACCGTAGTTATGGGTCGTAAAACTTTTGATTCAATTGGAAAAGCGT
TGCCTAACCGAAAAAATATTGTTTTAAGTCATCATCCAACCTCATTGCCAGATTCTGTAGTTGGCGTGGGTTCATTAAGT
GAGCTTCAGGCGATTTTTGAAACCCATCCTAATGAAAAATTTTATATTATTGGAGGAAGTCATTTATACAATGCCTTGTT
ATCACAAGCTGATGAACTTCTGATAACTAGAATCCAAAAAGATTATCAATGTGAGGTTTTAGCACCTGATATCACCCAAA
ATGAATTTAAATTGGCTAGCTCAGTTGAACATGAATCAACCGAAAGAAATCCTGCATATACTTTTGAAACATGGATTAGA
ATTTAA

Upstream 100 bases:

>100_bases
TATGAACCAGGAGATATTGTGGTTGAAGGATATGATCCTCATCCAACCATTAAAGCACCGGTAGCAGTTTAATTTTAAGT
CAATTTAGGAGGAATTAAAT

Downstream 100 bases:

>100_bases
TAAACGTTAAAACCTCATTTATTGAGGTTTTTTATTTATCCTCCTTTTCAAAATTAAAATTATATGATATATTTAATCTA
ATTTTTAATGTTAGGTTATC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MISMIWAEDLRHGIGKQQSIPWHIPGDMAFFKKTTSGNTVVMGRKTFDSIGKALPNRKNIVLSHHPTSLPDSVVGVGSLS
ELQAIFETHPNEKFYIIGGSHLYNALLSQADELLITRIQKDYQCEVLAPDITQNEFKLASSVEHESTERNPAYTFETWIR
I

Sequences:

>Translated_161_residues
MISMIWAEDLRHGIGKQQSIPWHIPGDMAFFKKTTSGNTVVMGRKTFDSIGKALPNRKNIVLSHHPTSLPDSVVGVGSLS
ELQAIFETHPNEKFYIIGGSHLYNALLSQADELLITRIQKDYQCEVLAPDITQNEFKLASSVEHESTERNPAYTFETWIR
I
>Mature_161_residues
MISMIWAEDLRHGIGKQQSIPWHIPGDMAFFKKTTSGNTVVMGRKTFDSIGKALPNRKNIVLSHHPTSLPDSVVGVGSLS
ELQAIFETHPNEKFYIIGGSHLYNALLSQADELLITRIQKDYQCEVLAPDITQNEFKLASSVEHESTERNPAYTFETWIR
I

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=157, Percent_Identity=35.6687898089172, Blast_Score=99, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17505899, Length=142, Percent_Identity=33.8028169014084, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324810, Length=162, Percent_Identity=32.7160493827161, Blast_Score=71, Evalue=7e-14,
Organism=Drosophila melanogaster, GI116008026, Length=134, Percent_Identity=32.8358208955224, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24647458, Length=134, Percent_Identity=32.8358208955224, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18140; Mature: 18140

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMIWAEDLRHGIGKQQSIPWHIPGDMAFFKKTTSGNTVVMGRKTFDSIGKALPNRKNI
CCCCHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCEEEEECHHHHHHHHHCCCCCCE
VLSHHPTSLPDSVVGVGSLSELQAIFETHPNEKFYIIGGSHLYNALLSQADELLITRIQK
EEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCC
DYQCEVLAPDITQNEFKLASSVEHESTERNPAYTFETWIRI
CCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEHEEEEEC
>Mature Secondary Structure
MISMIWAEDLRHGIGKQQSIPWHIPGDMAFFKKTTSGNTVVMGRKTFDSIGKALPNRKNI
CCCCHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCEEEEECHHHHHHHHHCCCCCCE
VLSHHPTSLPDSVVGVGSLSELQAIFETHPNEKFYIIGGSHLYNALLSQADELLITRIQK
EEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCC
DYQCEVLAPDITQNEFKLASSVEHESTERNPAYTFETWIRI
CCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]