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The map label for this gene is alsS [H]

Identifier: 116492208

GI number: 116492208

Start: 452245

End: 453921

Strand: Direct

Name: alsS [H]

Synonym: PEPE_0405

Alternate gene names: 116492208

Gene position: 452245-453921 (Clockwise)

Preceding gene: 116492207

Following gene: 116492209

Centisome position: 24.68

GC content: 42.7

Gene sequence:

>1677_bases
ATGATTCAAAGTAGTTATACCGGGTCAGATTCAATTCTCGATAGTTTAATCAACCATGATGTACCATATATTTTTGGTAT
TCCTGGAGCCAAAATTGATTTGTTATTTGAACAACTGGAACATTCTAACCGAGCAAAAAAACCTAAATTAATTATTACAA
GACATGAACAAAATGCGGCATTTATGGCGGCTGGTGTTGGACGAATTACTGGTAAACCGGGAGTAGTGCTTACTACTTCA
GGACCTGGCGTGGGAAACCTTGCCACGGGATTACTAACGGCTACGGCAGAAGGTGATCCAGTATTAGCCATTGGGGGCCA
AGTACCACGAAATGACTTATTGCGTTTAACTCATCAAAGTATGCGGAACGCTGCTTTATTTGAACCCATCACTAAGTTTA
GTGTGGAAGTTCAAGAACCAGAAAACATTTCCGAAGTTTTAGCAAATGCTTACCGGGCAGCTGAATCATCTAAGCAGGGA
GCTAGTTTTGTTAGTTTTCCACAAGATGTGGTTAATGCACCGGTTAGTTCGCGTCCAATCAAACCTTTAAAAAAACCGAT
TCTCGGACCGGCGAGCCCGATTGAAATTGAAGAGCTAGCTAAAGCCATTCGAAACGCTCAACTTCCCGTATTGCTACTCG
GGATGCGGGCCTCGTCTCCCGAGGTGACAAAAGCCGTCCGCGAATTAGTACGGATTGCTAACTTACCTGTTGTGGAAACC
TTCCAAGGTGCTGGTATTATTTCTCATGACTTAGAAGATCATTTTTACGGTCGAGTGGGGTTATTCCGCAATCAGCCTGG
GGATAAGTTATTGAAGCATAGTGATTTGGTAATCACAGTGGGTTACGATGCCATCGAATATGAACCTCGTAACTGGAACG
CAGAAGGGGATGGCAAGATTATCGTCATTGACGATTCGCCGTCTGAAATTGATCATAACTTTGAACCTGAAAAGGAATTA
GTTGGGGACATCGCGCAAACGATTGATTTGCTAACTCCGCATCTTAAAGATTACCAAGTTGGTGTTGATGGTGAACAATA
CTTGGAAACACTCCGTGAGCTGCAAAACAAGCAAGACTTGCCACCAGTGCCGAAGCAAGATTCCAAACGTATTCATCCAT
TGTCTATTATTTCTGCACTTCAATATCGGGTTGACGATGAAATGACTGTCTCCGTTGATGTGGGAAGTTTCTACATTTGG
ATGGCTCGTCACTTCAGAAGTTATCAGCCTCGTCATTTGCTATTTAGTAACGGGATGCAAACCTTAGGAGTAGCATTGCC
ATGGGCCATTTCGGCCGCTTTGTTACGTCCTAATAGTAAGTCAGTTTCAGTTTCAGGAGATGGTGGATTCCTTTTCTCTG
CGCAAGAATTAGAAACGGCTGTCCGTGAAAAGTTAGATATTGTTCATATTATTTGGAACGATGGATACTACGATATGGTT
AAGTTTCAAGAAGAAATTAAGTATGGACATTCTGCAGGCGTTGACTTTGGCCCCGTAGATTTTGTTAAATTGGCTGAAGG
ATATGGCGCTAAAGGATTGCGTGTGGAAAAACCAGAGGACTTGGGTAAAATTCTTGACGAAGCATTTCAAACAGAAGGAC
CAGTAGTGGTGGATATCCCAGTTGATTATTCCGATAACGTTAAGCTAGGGCAATCACTTCTTCCTGACCAATTATAG

Upstream 100 bases:

>100_bases
AAAGAGCTTAAAATAAGATACTTTTTTATTAAGCTCTTTTTATTATATTTTGATATAGTTAACTATGTAATCACTTTCAA
AAACCAAAGGAGAAAAAATA

Downstream 100 bases:

>100_bases
GAGGGATTTAAATGGCAAAATTATTTCAACATGGGACGCTCGCAATGCTAGTAGATGGACTTTTTGGTGGGACGCTTGCG
GTTTCCGATTTACTGAAGCA

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MIQSSYTGSDSILDSLINHDVPYIFGIPGAKIDLLFEQLEHSNRAKKPKLIITRHEQNAAFMAAGVGRITGKPGVVLTTS
GPGVGNLATGLLTATAEGDPVLAIGGQVPRNDLLRLTHQSMRNAALFEPITKFSVEVQEPENISEVLANAYRAAESSKQG
ASFVSFPQDVVNAPVSSRPIKPLKKPILGPASPIEIEELAKAIRNAQLPVLLLGMRASSPEVTKAVRELVRIANLPVVET
FQGAGIISHDLEDHFYGRVGLFRNQPGDKLLKHSDLVITVGYDAIEYEPRNWNAEGDGKIIVIDDSPSEIDHNFEPEKEL
VGDIAQTIDLLTPHLKDYQVGVDGEQYLETLRELQNKQDLPPVPKQDSKRIHPLSIISALQYRVDDEMTVSVDVGSFYIW
MARHFRSYQPRHLLFSNGMQTLGVALPWAISAALLRPNSKSVSVSGDGGFLFSAQELETAVREKLDIVHIIWNDGYYDMV
KFQEEIKYGHSAGVDFGPVDFVKLAEGYGAKGLRVEKPEDLGKILDEAFQTEGPVVVDIPVDYSDNVKLGQSLLPDQL

Sequences:

>Translated_558_residues
MIQSSYTGSDSILDSLINHDVPYIFGIPGAKIDLLFEQLEHSNRAKKPKLIITRHEQNAAFMAAGVGRITGKPGVVLTTS
GPGVGNLATGLLTATAEGDPVLAIGGQVPRNDLLRLTHQSMRNAALFEPITKFSVEVQEPENISEVLANAYRAAESSKQG
ASFVSFPQDVVNAPVSSRPIKPLKKPILGPASPIEIEELAKAIRNAQLPVLLLGMRASSPEVTKAVRELVRIANLPVVET
FQGAGIISHDLEDHFYGRVGLFRNQPGDKLLKHSDLVITVGYDAIEYEPRNWNAEGDGKIIVIDDSPSEIDHNFEPEKEL
VGDIAQTIDLLTPHLKDYQVGVDGEQYLETLRELQNKQDLPPVPKQDSKRIHPLSIISALQYRVDDEMTVSVDVGSFYIW
MARHFRSYQPRHLLFSNGMQTLGVALPWAISAALLRPNSKSVSVSGDGGFLFSAQELETAVREKLDIVHIIWNDGYYDMV
KFQEEIKYGHSAGVDFGPVDFVKLAEGYGAKGLRVEKPEDLGKILDEAFQTEGPVVVDIPVDYSDNVKLGQSLLPDQL
>Mature_558_residues
MIQSSYTGSDSILDSLINHDVPYIFGIPGAKIDLLFEQLEHSNRAKKPKLIITRHEQNAAFMAAGVGRITGKPGVVLTTS
GPGVGNLATGLLTATAEGDPVLAIGGQVPRNDLLRLTHQSMRNAALFEPITKFSVEVQEPENISEVLANAYRAAESSKQG
ASFVSFPQDVVNAPVSSRPIKPLKKPILGPASPIEIEELAKAIRNAQLPVLLLGMRASSPEVTKAVRELVRIANLPVVET
FQGAGIISHDLEDHFYGRVGLFRNQPGDKLLKHSDLVITVGYDAIEYEPRNWNAEGDGKIIVIDDSPSEIDHNFEPEKEL
VGDIAQTIDLLTPHLKDYQVGVDGEQYLETLRELQNKQDLPPVPKQDSKRIHPLSIISALQYRVDDEMTVSVDVGSFYIW
MARHFRSYQPRHLLFSNGMQTLGVALPWAISAALLRPNSKSVSVSGDGGFLFSAQELETAVREKLDIVHIIWNDGYYDMV
KFQEEIKYGHSAGVDFGPVDFVKLAEGYGAKGLRVEKPEDLGKILDEAFQTEGPVVVDIPVDYSDNVKLGQSLLPDQL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=28.2456140350877, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI21361361, Length=586, Percent_Identity=24.5733788395904, Blast_Score=152, Evalue=6e-37,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=28.9330922242315, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=27.787610619469, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=26.8551236749117, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787096, Length=536, Percent_Identity=27.4253731343284, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788716, Length=563, Percent_Identity=25.3996447602131, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=561, Percent_Identity=26.9162210338681, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=561, Percent_Identity=26.9162210338681, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=574, Percent_Identity=25.0871080139373, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=29.4014084507042, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320816, Length=552, Percent_Identity=25.7246376811594, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=24.6017699115044, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61264; Mature: 61264

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSSYTGSDSILDSLINHDVPYIFGIPGAKIDLLFEQLEHSNRAKKPKLIITRHEQNAA
CCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCE
FMAAGVGRITGKPGVVLTTSGPGVGNLATGLLTATAEGDPVLAIGGQVPRNDLLRLTHQS
EEECCCCCCCCCCCEEEEECCCCCCHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHH
MRNAALFEPITKFSVEVQEPENISEVLANAYRAAESSKQGASFVSFPQDVVNAPVSSRPI
HHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHCCCHHHHCCCCCCCCC
KPLKKPILGPASPIEIEELAKAIRNAQLPVLLLGMRASSPEVTKAVRELVRIANLPVVET
CHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEE
FQGAGIISHDLEDHFYGRVGLFRNQPGDKLLKHSDLVITVGYDAIEYEPRNWNAEGDGKI
CCCCCCCHHCHHHHHCCCEEEECCCCHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCEE
IVIDDSPSEIDHNFEPEKELVGDIAQTIDLLTPHLKDYQVGVDGEQYLETLRELQNKQDL
EEECCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCC
PPVPKQDSKRIHPLSIISALQYRVDDEMTVSVDVGSFYIWMARHFRSYQPRHLLFSNGMQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHCCHH
TLGVALPWAISAALLRPNSKSVSVSGDGGFLFSAQELETAVREKLDIVHIIWNDGYYDMV
HHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCHHHH
KFQEEIKYGHSAGVDFGPVDFVKLAEGYGAKGLRVEKPEDLGKILDEAFQTEGPVVVDIP
HHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCEEEEEC
VDYSDNVKLGQSLLPDQL
CCCCCCCHHHHHCCCCCC
>Mature Secondary Structure
MIQSSYTGSDSILDSLINHDVPYIFGIPGAKIDLLFEQLEHSNRAKKPKLIITRHEQNAA
CCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCE
FMAAGVGRITGKPGVVLTTSGPGVGNLATGLLTATAEGDPVLAIGGQVPRNDLLRLTHQS
EEECCCCCCCCCCCEEEEECCCCCCHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHH
MRNAALFEPITKFSVEVQEPENISEVLANAYRAAESSKQGASFVSFPQDVVNAPVSSRPI
HHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHCCCHHHHCCCCCCCCC
KPLKKPILGPASPIEIEELAKAIRNAQLPVLLLGMRASSPEVTKAVRELVRIANLPVVET
CHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEE
FQGAGIISHDLEDHFYGRVGLFRNQPGDKLLKHSDLVITVGYDAIEYEPRNWNAEGDGKI
CCCCCCCHHCHHHHHCCCEEEECCCCHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCEE
IVIDDSPSEIDHNFEPEKELVGDIAQTIDLLTPHLKDYQVGVDGEQYLETLRELQNKQDL
EEECCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCC
PPVPKQDSKRIHPLSIISALQYRVDDEMTVSVDVGSFYIWMARHFRSYQPRHLLFSNGMQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHCCHH
TLGVALPWAISAALLRPNSKSVSVSGDGGFLFSAQELETAVREKLDIVHIIWNDGYYDMV
HHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCHHHH
KFQEEIKYGHSAGVDFGPVDFVKLAEGYGAKGLRVEKPEDLGKILDEAFQTEGPVVVDIP
HHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCEEEEEC
VDYSDNVKLGQSLLPDQL
CCCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]