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The map label for this gene is purR [H]

Identifier: 116492096

GI number: 116492096

Start: 336603

End: 337439

Strand: Direct

Name: purR [H]

Synonym: PEPE_0292

Alternate gene names: 116492096

Gene position: 336603-337439 (Clockwise)

Preceding gene: 116492095

Following gene: 116492097

Centisome position: 18.37

GC content: 39.31

Gene sequence:

>837_bases
ATGAAATTGAAGCGAAGTGAACGATTGATTGATATGACGAACTATTTAATCAATCATCCAAGAACGCTCATAACTTCTGG
ATTTTTTGTTACAAGATATTCATCCGCTAAATCTTCTATTAGCGAAGATTTATCCATTATGAAATCAACTTTAGAAAAAC
AACAACTAGGAACCATTGAAATTACTAATGGGGCTGCTGGTGGTATTAAATATCTTCCGGGAATTGGATCAGAGGAGGCT
CACAAAGTTGTTGATGAGTTAACTGAACAACTAGCGGATCCAAGTCGTAAACTACCTGGTGGGTATCTTTACATGTCTGA
TTTGTTAGGCCATCCCAGTACCTTACGGGCCATTGGCCGAATGTTAGCTAGCGAATATATGCGTTCTGATGTTGATGCAG
TTATGACTGTGGAAACTAAAGGGATTCCACTAGCTCAAGCGGTTGCGAACTATTTAAATGTTCCATTTATTATTGTGCGT
CATGAATCCGTGATTACCGAAGGTTCGACTATTTCAGTTAACTACGTTTCACGTTCATCAGAACGTATCCAAAAAATGGA
ATTGTCTAAACGTAGTTTACAGCGAGGAATGAAAGTTTTAATCGTGGATGATTACATGAAGGGTGGCGGCACGGTTCATG
CCATGGTTGATTTACTGAAAGAATTTGAAGCTGAATTAACCGGCATTGCCGTCTTCGCGGAAGGTACATACAAGGGACAA
CGTGCGATTGAAGACTTTACTTCCTTGTTAAATGTCGATACATCTCAAGATGGACAGGTCCAAATTAAGGCCGGAAATTA
TTTTGAAAGAAACTTTAAGGATATTGAGGAGCGCTAG

Upstream 100 bases:

>100_bases
TCCGAACATTATTAATGAACTATGATATTATATTCATTGGTAAGTAAAAAGTAAGTAATTTTTTGCGAATAATACGCAAA
AGAAGAGTGGAGTGAAACCA

Downstream 100 bases:

>100_bases
TAATAATGGAAAAAAATACAATCATTTTAGCTGCAGGTCAAGGTACTCGAATGAAATCAAAACTATATAAAGTTTTGCAT
CAAGTTTGTGGAAAAGCCAT

Product: pur operon repressor

Products: AMP; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKLKRSERLIDMTNYLINHPRTLITSGFFVTRYSSAKSSISEDLSIMKSTLEKQQLGTIEITNGAAGGIKYLPGIGSEEA
HKVVDELTEQLADPSRKLPGGYLYMSDLLGHPSTLRAIGRMLASEYMRSDVDAVMTVETKGIPLAQAVANYLNVPFIIVR
HESVITEGSTISVNYVSRSSERIQKMELSKRSLQRGMKVLIVDDYMKGGGTVHAMVDLLKEFEAELTGIAVFAEGTYKGQ
RAIEDFTSLLNVDTSQDGQVQIKAGNYFERNFKDIEER

Sequences:

>Translated_278_residues
MKLKRSERLIDMTNYLINHPRTLITSGFFVTRYSSAKSSISEDLSIMKSTLEKQQLGTIEITNGAAGGIKYLPGIGSEEA
HKVVDELTEQLADPSRKLPGGYLYMSDLLGHPSTLRAIGRMLASEYMRSDVDAVMTVETKGIPLAQAVANYLNVPFIIVR
HESVITEGSTISVNYVSRSSERIQKMELSKRSLQRGMKVLIVDDYMKGGGTVHAMVDLLKEFEAELTGIAVFAEGTYKGQ
RAIEDFTSLLNVDTSQDGQVQIKAGNYFERNFKDIEER
>Mature_278_residues
MKLKRSERLIDMTNYLINHPRTLITSGFFVTRYSSAKSSISEDLSIMKSTLEKQQLGTIEITNGAAGGIKYLPGIGSEEA
HKVVDELTEQLADPSRKLPGGYLYMSDLLGHPSTLRAIGRMLASEYMRSDVDAVMTVETKGIPLAQAVANYLNVPFIIVR
HESVITEGSTISVNYVSRSSERIQKMELSKRSLQRGMKVLIVDDYMKGGGTVHAMVDLLKEFEAELTGIAVFAEGTYKGQ
RAIEDFTSLLNVDTSQDGQVQIKAGNYFERNFKDIEER

Specific function: Controls the transcription of the pur operon for purine biosynthetic genes, binds to the control region of the operon. DNA binding is inhibited by 5-phosphoribosyl 1-pyrophosphate [H]

COG id: COG0503

COG function: function code F; Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000836
- InterPro:   IPR015265
- InterPro:   IPR010078
- InterPro:   IPR011991
- ProDom:   PD029816 [H]

Pfam domain/function: PF00156 Pribosyltran; PF09182 PuR_N [H]

EC number: 2.4.2.7 [C]

Molecular weight: Translated: 30900; Mature: 30900

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKRSERLIDMTNYLINHPRTLITSGFFVTRYSSAKSSISEDLSIMKSTLEKQQLGTIE
CCCCHHHHHHHHHHHHHHCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCEEE
ITNGAAGGIKYLPGIGSEEAHKVVDELTEQLADPSRKLPGGYLYMSDLLGHPSTLRAIGR
ECCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHCCCHHHHHHHH
MLASEYMRSDVDAVMTVETKGIPLAQAVANYLNVPFIIVRHESVITEGSTISVNYVSRSS
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCHHEECCCEEEEEEECCHH
ERIQKMELSKRSLQRGMKVLIVDDYMKGGGTVHAMVDLLKEFEAELTGIAVFAEGTYKGQ
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCHH
RAIEDFTSLLNVDTSQDGQVQIKAGNYFERNFKDIEER
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MKLKRSERLIDMTNYLINHPRTLITSGFFVTRYSSAKSSISEDLSIMKSTLEKQQLGTIE
CCCCHHHHHHHHHHHHHHCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCEEE
ITNGAAGGIKYLPGIGSEEAHKVVDELTEQLADPSRKLPGGYLYMSDLLGHPSTLRAIGR
ECCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHCCCHHHHHHHH
MLASEYMRSDVDAVMTVETKGIPLAQAVANYLNVPFIIVRHESVITEGSTISVNYVSRSS
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCHHEECCCEEEEEEECCHH
ERIQKMELSKRSLQRGMKVLIVDDYMKGGGTVHAMVDLLKEFEAELTGIAVFAEGTYKGQ
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCHH
RAIEDFTSLLNVDTSQDGQVQIKAGNYFERNFKDIEER
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 560 [C]

Specific activity: 14

Km value (mM): 0.02 {adenine}} 0.125 {5-phospho-alpha-D-ribose} [C]

Substrates: Adenine; 5-Phospho-alpha-D-ribose 1-diphosphate [C]

Specific reaction: Adenine + 5-Phospho-alpha-D-ribose 1-diphosphate --> AMP + Diphosphate [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 7638212 [H]