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The map label for this gene is pfkA [H]

Identifier: 116332530

GI number: 116332530

Start: 107692

End: 109026

Strand: Direct

Name: pfkA [H]

Synonym: LBJ_4094

Alternate gene names: 116332530

Gene position: 107692-109026 (Clockwise)

Preceding gene: 116332528

Following gene: 116332533

Centisome position: 35.93

GC content: 44.27

Gene sequence:

>1335_bases
ATGGAAACACGAATTCAAAATTTTGGTCAATGTTCGATATCTAGTCCCGCGAATTATGAATATTACACGAGTGATTCCTC
CAGGTTGATATTTAAAACTGTGTTTCAATCGGAAGAGGATTGGAATTCTTATGTGAAAGGTAATCCCGATTTTTTCGAAC
AGGCTGGACCTAGGGAAAAAATATATTTTTCTCCCAAAGAAGTGACCGCCGGAATCGTAACTTGCGGAGGTTTGTGCCCC
GGAATCAACGACGTGATTCGAGGGATAGTAATGGAGCTTTATTATCGTTACGGAGTTTCCAGGATTTTAGGTTTTCCCTA
CGGCTACCAAGGGCTTGTAAAAAAATATTCTTACGAACCGATTGAACTCACGCCTGAAAAAGTGGCGCATATTGCGGATG
AAGGCGGGTCTATGCTCTCTTCGTCCAGAGGAAATCAATCTCCGGTCGAAATGGTGGATTGTCTCGGCTTTTACGGAGTA
AAGATGCTTTTTTGTATTGGAGGAGACGGCACTCTTCGAGGCGCTCGGGAGATCGTGAATGAGGTCGCGAGAAGAAAGAT
GGAAATTTCTGTCATTGGAATTCCGAAGACGATCGACAACGATATCAACTACGTACAAAAAACATTCGGTTTTTCTACTG
CGTTTTCCAAGGCGATGGAAGCGGTGGAATGTGGACATGTGGAAGCGAAAGGAGCGCCGAACGGAATCGGCTTGGTCAAA
CTGATGGGACGTCATTCCGGATTCATCGCGGTAAATGCGGCTCTCGCTTCTAAAAACGTAAATTATTGCCTAATTCCCGA
AGTGAATTTTGACCTCTATGGAAATGGAGCGTTTTTGGACGATCTCAAAAAAAGAATCCAAAAGAAGGGACACGCGGTTA
TCATCGTGGCGGAAGGAGCCGGGCAGAAATTTTTCGATTCGACGGAAGAAAGAGACCCTTCCGGGAATCTGAAACTGGGG
GATATTGGTCTTTTCCTCAAGGATACGATGACTGAGTTTTTCAAAAAAGAAAACATTCCAGTGAACATCAAATACATAGA
TCCGAGTTATATCATTCGATCGATTCCGGCTAACGCGGAAGATTCCGTATTCTGTGGTTTTTTGGCTCAAAACGCGGTTC
ACGCGGCAATGGCCGGTAAAACCGATATGGTTGTGGGAATGTGGAACAACGTGTTCATACATCTTCCGATTTCTCTTGCG
ATTCAGGAAAGAAAAGTGTTGCAACCGGACAAAAGTACTCTTTGGAGATCTCTTTTGGCTTCCACGGGTCAGCCCGCGCA
TATGTTGGCCAAACATTCAGAGGACAGAAATGTAAATTACGTCAGAATCATTTGA

Upstream 100 bases:

>100_bases
CTGTAAGACTATGGAAATAGAACAGAACTGTCAGTATAAAATTGCAAAATGAGGTGATTTTTACGGGGATTCTCGAAACT
ATTTGTGTGAGGACCCCGTT

Downstream 100 bases:

>100_bases
TACACCAAGGAATATCGTTTTACAATTGCTTCCCGTAGGGAAGCCCAGCGGCTTTGCTGCTTCTGTCCTCTGTCTAGAAC
GAGCGAAGCGAGTTCGTCCG

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
METRIQNFGQCSISSPANYEYYTSDSSRLIFKTVFQSEEDWNSYVKGNPDFFEQAGPREKIYFSPKEVTAGIVTCGGLCP
GINDVIRGIVMELYYRYGVSRILGFPYGYQGLVKKYSYEPIELTPEKVAHIADEGGSMLSSSRGNQSPVEMVDCLGFYGV
KMLFCIGGDGTLRGAREIVNEVARRKMEISVIGIPKTIDNDINYVQKTFGFSTAFSKAMEAVECGHVEAKGAPNGIGLVK
LMGRHSGFIAVNAALASKNVNYCLIPEVNFDLYGNGAFLDDLKKRIQKKGHAVIIVAEGAGQKFFDSTEERDPSGNLKLG
DIGLFLKDTMTEFFKKENIPVNIKYIDPSYIIRSIPANAEDSVFCGFLAQNAVHAAMAGKTDMVVGMWNNVFIHLPISLA
IQERKVLQPDKSTLWRSLLASTGQPAHMLAKHSEDRNVNYVRII

Sequences:

>Translated_444_residues
METRIQNFGQCSISSPANYEYYTSDSSRLIFKTVFQSEEDWNSYVKGNPDFFEQAGPREKIYFSPKEVTAGIVTCGGLCP
GINDVIRGIVMELYYRYGVSRILGFPYGYQGLVKKYSYEPIELTPEKVAHIADEGGSMLSSSRGNQSPVEMVDCLGFYGV
KMLFCIGGDGTLRGAREIVNEVARRKMEISVIGIPKTIDNDINYVQKTFGFSTAFSKAMEAVECGHVEAKGAPNGIGLVK
LMGRHSGFIAVNAALASKNVNYCLIPEVNFDLYGNGAFLDDLKKRIQKKGHAVIIVAEGAGQKFFDSTEERDPSGNLKLG
DIGLFLKDTMTEFFKKENIPVNIKYIDPSYIIRSIPANAEDSVFCGFLAQNAVHAAMAGKTDMVVGMWNNVFIHLPISLA
IQERKVLQPDKSTLWRSLLASTGQPAHMLAKHSEDRNVNYVRII
>Mature_444_residues
METRIQNFGQCSISSPANYEYYTSDSSRLIFKTVFQSEEDWNSYVKGNPDFFEQAGPREKIYFSPKEVTAGIVTCGGLCP
GINDVIRGIVMELYYRYGVSRILGFPYGYQGLVKKYSYEPIELTPEKVAHIADEGGSMLSSSRGNQSPVEMVDCLGFYGV
KMLFCIGGDGTLRGAREIVNEVARRKMEISVIGIPKTIDNDINYVQKTFGFSTAFSKAMEAVECGHVEAKGAPNGIGLVK
LMGRHSGFIAVNAALASKNVNYCLIPEVNFDLYGNGAFLDDLKKRIQKKGHAVIIVAEGAGQKFFDSTEERDPSGNLKLG
DIGLFLKDTMTEFFKKENIPVNIKYIDPSYIIRSIPANAEDSVFCGFLAQNAVHAAMAGKTDMVVGMWNNVFIHLPISLA
IQERKVLQPDKSTLWRSLLASTGQPAHMLAKHSEDRNVNYVRII

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=363, Percent_Identity=25.3443526170799, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI266453768, Length=363, Percent_Identity=25.3443526170799, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI266453748, Length=363, Percent_Identity=25.3443526170799, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI4505749, Length=363, Percent_Identity=25.3443526170799, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI11321601, Length=255, Percent_Identity=29.0196078431373, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI48762920, Length=382, Percent_Identity=24.6073298429319, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1790350, Length=341, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25147584, Length=403, Percent_Identity=24.317617866005, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558788, Length=269, Percent_Identity=30.4832713754647, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321679, Length=381, Percent_Identity=24.6719160104987, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323861, Length=264, Percent_Identity=27.6515151515151, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28573326, Length=265, Percent_Identity=26.0377358490566, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652337, Length=261, Percent_Identity=26.0536398467433, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 49143; Mature: 49143

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRIQNFGQCSISSPANYEYYTSDSSRLIFKTVFQSEEDWNSYVKGNPDFFEQAGPREK
CCCCCCCCCCEECCCCCCCEEEECCCCCEEHHHHHCCCHHHHHHCCCCCHHHHHCCCCCE
IYFSPKEVTAGIVTCGGLCPGINDVIRGIVMELYYRYGVSRILGFPYGYQGLVKKYSYEP
EEECHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
IELTPEKVAHIADEGGSMLSSSRGNQSPVEMVDCLGFYGVKMLFCIGGDGTLRGAREIVN
CEECHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHH
EVARRKMEISVIGIPKTIDNDINYVQKTFGFSTAFSKAMEAVECGHVEAKGAPNGIGLVK
HHHHCEEEEEEEECCCHHCCHHHHHHHHHCHHHHHHHHHHHHHCCCEECCCCCCCCCCEE
LMGRHSGFIAVNAALASKNVNYCLIPEVNFDLYGNGAFLDDLKKRIQKKGHAVIIVAEGA
EECCCCCEEEEEEEHHCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHCCCEEEEEECCC
GQKFFDSTEERDPSGNLKLGDIGLFLKDTMTEFFKKENIPVNIKYIDPSYIIRSIPANAE
CCHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCC
DSVFCGFLAQNAVHAAMAGKTDMVVGMWNNVFIHLPISLAIQERKVLQPDKSTLWRSLLA
CCEEHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECHHHHCCCCHHHHHHHHHH
STGQPAHMLAKHSEDRNVNYVRII
CCCCCHHHHHHCCCCCCCCEEEEC
>Mature Secondary Structure
METRIQNFGQCSISSPANYEYYTSDSSRLIFKTVFQSEEDWNSYVKGNPDFFEQAGPREK
CCCCCCCCCCEECCCCCCCEEEECCCCCEEHHHHHCCCHHHHHHCCCCCHHHHHCCCCCE
IYFSPKEVTAGIVTCGGLCPGINDVIRGIVMELYYRYGVSRILGFPYGYQGLVKKYSYEP
EEECHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
IELTPEKVAHIADEGGSMLSSSRGNQSPVEMVDCLGFYGVKMLFCIGGDGTLRGAREIVN
CEECHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHH
EVARRKMEISVIGIPKTIDNDINYVQKTFGFSTAFSKAMEAVECGHVEAKGAPNGIGLVK
HHHHCEEEEEEEECCCHHCCHHHHHHHHHCHHHHHHHHHHHHHCCCEECCCCCCCCCCEE
LMGRHSGFIAVNAALASKNVNYCLIPEVNFDLYGNGAFLDDLKKRIQKKGHAVIIVAEGA
EECCCCCEEEEEEEHHCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHCCCEEEEEECCC
GQKFFDSTEERDPSGNLKLGDIGLFLKDTMTEFFKKENIPVNIKYIDPSYIIRSIPANAE
CCHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCC
DSVFCGFLAQNAVHAAMAGKTDMVVGMWNNVFIHLPISLAIQERKVLQPDKSTLWRSLLA
CCEEHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECHHHHCCCCHHHHHHHHHH
STGQPAHMLAKHSEDRNVNYVRII
CCCCCHHHHHHCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA