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The map label for this gene is pheA [H]

Identifier: 116328727

GI number: 116328727

Start: 2460522

End: 2461613

Strand: Reverse

Name: pheA [H]

Synonym: LBL_2101

Alternate gene names: 116328727

Gene position: 2461613-2460522 (Counterclockwise)

Preceding gene: 116328728

Following gene: 116328726

Centisome position: 68.1

GC content: 45.88

Gene sequence:

>1092_bases
ATGAGTGATTTGGATCAACAACTCAGAATTTTTCGGGATCAGATTGATTCCCTCGATCGGGAGATCGTAAAAGCGATTCA
GACTCGAGCCGAATTTGCATCCAAGATCGGGGAGATCAAACGAGAACGAAATGAACCGGTTTTCCGTCCTGATCGGGAAA
AGGAAGTTTACGAAAAGATAAAATCTTTGAGTTCGGGTCTTCTTCCCGACAAAGTGATGCTTGCGATCTACCGAGAGATC
ATGTCCGGTTCTATCTCTGTGGAAAAAGGATTGGAGGTAGGGTACCTCGGACCTGCGGGGTCTTTTTCCAACCAGGCGGT
TCGCACTCGTTTCGGAGCTTCGGTTAACGCGTTGGAATTCAATTCGATTCCGGACGTGTTTCGTGCTGTGGAAACCGATA
AGATAGACTACGGAGTCGTACCAGTGGAAAACTCCAGCGAAGGTTTGGTCAATTCGACTTTGGATCAATTTTTGATTTCC
GATCTTCTCATTTATTCCGAACACTATTTAAGAATCAATATCAGTCTTCTCGGATTTGAACACGATCTTTCCAAAATCAA
ATCGTTATACGGAATCAAAATCGCCAATTCCCAGTGTAAGAACTGGCTTGCGGCCAATCTACCTCACGTGGAAATCGTAG
AAACGTCTTCCACGGCCAAGGCGGCTCAGATCGTAGCGGAAAAAAAAGAGGCTTGTGCGGCCATTGCCTCCTCCATTGCG
GCGGAAATCTACGGGCTCAGTCTGATCCGGGAATCCATTGAAGACCTCGTCGACAATACGACGCGATTTCTCATCATCGG
AAAAAATCAATGCCCACCCACCGGAAACGACAAAACTTCGATCGTTTTCTCTTGTCCCGATAAACCGGGCGCGCTTTATC
GGGTACTAAAACCGTTTTTCGATCATCAGTTGAATCTGACAAAGATCGAATCAAGACCCACGAGAAGGAACTCTTGGGAA
TACAACTTCTTTATCGACTTTTACGGTCATCAAAAGGACGAAACGATTCAGAACGTCCTCTCAAATCTGAAGGAAAATAC
AATTTTTCTGAGAACGCTCGGCTCCTATCCGATGTCTTCGCAAAGCCTGTGA

Upstream 100 bases:

>100_bases
GAATTCTTGATTCATTTTGGATTGAATAAACTATCGGATCTTCCTGCGCCCGTGGAAGTTAAAGAATTGAAATTCGAAAA
TCTGGACGATCTACTCGAAC

Downstream 100 bases:

>100_bases
AATCAAAGTTTTCTAATATTCTTATATACGGATTAGGGCTTATGGGAGCTTCGCTTTCTCTTGCCCTCAAAAAAAAAGGA
ATTTCGGCGCACGTTACCGG

Product: bifunctional prephenate dehydratase/chorismate mutase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSDLDQQLRIFRDQIDSLDREIVKAIQTRAEFASKIGEIKRERNEPVFRPDREKEVYEKIKSLSSGLLPDKVMLAIYREI
MSGSISVEKGLEVGYLGPAGSFSNQAVRTRFGASVNALEFNSIPDVFRAVETDKIDYGVVPVENSSEGLVNSTLDQFLIS
DLLIYSEHYLRINISLLGFEHDLSKIKSLYGIKIANSQCKNWLAANLPHVEIVETSSTAKAAQIVAEKKEACAAIASSIA
AEIYGLSLIRESIEDLVDNTTRFLIIGKNQCPPTGNDKTSIVFSCPDKPGALYRVLKPFFDHQLNLTKIESRPTRRNSWE
YNFFIDFYGHQKDETIQNVLSNLKENTIFLRTLGSYPMSSQSL

Sequences:

>Translated_363_residues
MSDLDQQLRIFRDQIDSLDREIVKAIQTRAEFASKIGEIKRERNEPVFRPDREKEVYEKIKSLSSGLLPDKVMLAIYREI
MSGSISVEKGLEVGYLGPAGSFSNQAVRTRFGASVNALEFNSIPDVFRAVETDKIDYGVVPVENSSEGLVNSTLDQFLIS
DLLIYSEHYLRINISLLGFEHDLSKIKSLYGIKIANSQCKNWLAANLPHVEIVETSSTAKAAQIVAEKKEACAAIASSIA
AEIYGLSLIRESIEDLVDNTTRFLIIGKNQCPPTGNDKTSIVFSCPDKPGALYRVLKPFFDHQLNLTKIESRPTRRNSWE
YNFFIDFYGHQKDETIQNVLSNLKENTIFLRTLGSYPMSSQSL
>Mature_362_residues
SDLDQQLRIFRDQIDSLDREIVKAIQTRAEFASKIGEIKRERNEPVFRPDREKEVYEKIKSLSSGLLPDKVMLAIYREIM
SGSISVEKGLEVGYLGPAGSFSNQAVRTRFGASVNALEFNSIPDVFRAVETDKIDYGVVPVENSSEGLVNSTLDQFLISD
LLIYSEHYLRINISLLGFEHDLSKIKSLYGIKIANSQCKNWLAANLPHVEIVETSSTAKAAQIVAEKKEACAAIASSIAA
EIYGLSLIRESIEDLVDNTTRFLIIGKNQCPPTGNDKTSIVFSCPDKPGALYRVLKPFFDHQLNLTKIESRPTRRNSWEY
NFFIDFYGHQKDETIQNVLSNLKENTIFLRTLGSYPMSSQSL

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=377, Percent_Identity=29.4429708222812, Blast_Score=154, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6324013, Length=298, Percent_Identity=30.8724832214765, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 40833; Mature: 40702

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLDQQLRIFRDQIDSLDREIVKAIQTRAEFASKIGEIKRERNEPVFRPDREKEVYEKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KSLSSGLLPDKVMLAIYREIMSGSISVEKGLEVGYLGPAGSFSNQAVRTRFGASVNALEF
HHHHCCCCHHHHHHHHHHHHHCCCCCHHCCCEEEECCCCCCCCCHHHHHHHCCCCCEEEC
NSIPDVFRAVETDKIDYGVVPVENSSEGLVNSTLDQFLISDLLIYSEHYLRINISLLGFE
CCCHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCH
HDLSKIKSLYGIKIANSQCKNWLAANLPHVEIVETSSTAKAAQIVAEKKEACAAIASSIA
HHHHHHHHHHCEEECCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AEIYGLSLIRESIEDLVDNTTRFLIIGKNQCPPTGNDKTSIVFSCPDKPGALYRVLKPFF
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
DHQLNLTKIESRPTRRNSWEYNFFIDFYGHQKDETIQNVLSNLKENTIFLRTLGSYPMSS
HCCCCEEEECCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCCCC
QSL
CCC
>Mature Secondary Structure 
SDLDQQLRIFRDQIDSLDREIVKAIQTRAEFASKIGEIKRERNEPVFRPDREKEVYEKI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KSLSSGLLPDKVMLAIYREIMSGSISVEKGLEVGYLGPAGSFSNQAVRTRFGASVNALEF
HHHHCCCCHHHHHHHHHHHHHCCCCCHHCCCEEEECCCCCCCCCHHHHHHHCCCCCEEEC
NSIPDVFRAVETDKIDYGVVPVENSSEGLVNSTLDQFLISDLLIYSEHYLRINISLLGFE
CCCHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCH
HDLSKIKSLYGIKIANSQCKNWLAANLPHVEIVETSSTAKAAQIVAEKKEACAAIASSIA
HHHHHHHHHHCEEECCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AEIYGLSLIRESIEDLVDNTTRFLIIGKNQCPPTGNDKTSIVFSCPDKPGALYRVLKPFF
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
DHQLNLTKIESRPTRRNSWEYNFFIDFYGHQKDETIQNVLSNLKENTIFLRTLGSYPMSS
HCCCCEEEECCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCCCC
QSL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]