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The map label for this gene is fcl [H]

Identifier: 116327881

GI number: 116327881

Start: 1377451

End: 1378407

Strand: Direct

Name: fcl [H]

Synonym: LBL_1165

Alternate gene names: 116327881

Gene position: 1377451-1378407 (Clockwise)

Preceding gene: 116327880

Following gene: 116327882

Centisome position: 38.11

GC content: 42.32

Gene sequence:

>957_bases
ATGGAAAAAGACGCAAGAATTTACATTGCTGGTCATAAGGGACTTGTCGGTTCTGCTATCGAGCGAGTTTTGAAAAAAGA
AGGTTACGAGAATATTATTGGGAAATCTCACGCGGAGTTGGAGCTCACTGAGCAACCGAAGGTGAATGAATTTTTTGAAA
ATCGGAAACCTGAATATGTTTTCCTTGCCGCCGCAAAAGTAGGTGGAATCCACGCGAATAATACTTATCCGGCGGAATTC
ATCTTTTCTAATCTTCAAATTCAGAATAATATCATAGACGCTTGTTATCGGTTTAAAGTGAAAAAACTACTTTTTCTGGG
TTCTTCCTGTATTTATCCGAAGTTCGCCAAACAGCCGATGGATGAAGGTCAATTGTTGGACGGTAAACTGGAACCAACTA
ACGAACCCTATGCGATCGCAAAGATTGCAGGAATCGTAATGTGCCAGAGTTACAATCGTCAATACGGCACGAACTTTATT
TCCGTTATGCCGACCAATCTGTACGGTCCGGGCGACAATTATCATCCCGAGAATTCTCACGTTCTCCCGGCTTTGATTCG
TAGATTCTACGAAGCTAAAATTAAGGATCTTCCCGAAGTGGTCGTATGGGGAACGGGGAAACCCTTAAGGGAATTCTTAT
ATTCGGATGATATGGCTCGAGCCTGCGTCTTTTTAATGAAAAATTACGATGTTACCGGAGATGCGAAGGGCGGGGAACAT
GTGAATGTAGGCAGTGGCATCGAGATAAGTATTCGAGAATTGGCTGAAACGGTGAAAGAAGTAGTAGGTTATCAAGGACT
TCTGACTTTCGATCTTACGAAGCCAGACGGCACTCCTCGTAAACTTTTAGACGTTTCGAAACTTCATAAGATGGGTTGGA
AACACCAAGTGGAGTTAAAAGAGGGTATTCGGTTGGCGTTTGAGGATTTTTTGCGGAAAATAAATGCTTTAAAATAG

Upstream 100 bases:

>100_bases
CGTATTACGGTTCGTTATCTGAAGATGAAAATACAGGAATACGAACAAATCCAGAAAGAAATCGAGGAGTTAAAGAAGGA
AGTCGGGGAAGATTAAGGCG

Downstream 100 bases:

>100_bases
ATTTTACTGTATCGTTTAGTTTTATTTAATTTTTAAAAGAGGCTTTGGTTTTTAGAATATGTCAATAGATCTTCCTAATT
CAAAAAATGTCCTTCAATAT

Product: nucleoside-diphosphate-sugar epimerase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MEKDARIYIAGHKGLVGSAIERVLKKEGYENIIGKSHAELELTEQPKVNEFFENRKPEYVFLAAAKVGGIHANNTYPAEF
IFSNLQIQNNIIDACYRFKVKKLLFLGSSCIYPKFAKQPMDEGQLLDGKLEPTNEPYAIAKIAGIVMCQSYNRQYGTNFI
SVMPTNLYGPGDNYHPENSHVLPALIRRFYEAKIKDLPEVVVWGTGKPLREFLYSDDMARACVFLMKNYDVTGDAKGGEH
VNVGSGIEISIRELAETVKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELKEGIRLAFEDFLRKINALK

Sequences:

>Translated_318_residues
MEKDARIYIAGHKGLVGSAIERVLKKEGYENIIGKSHAELELTEQPKVNEFFENRKPEYVFLAAAKVGGIHANNTYPAEF
IFSNLQIQNNIIDACYRFKVKKLLFLGSSCIYPKFAKQPMDEGQLLDGKLEPTNEPYAIAKIAGIVMCQSYNRQYGTNFI
SVMPTNLYGPGDNYHPENSHVLPALIRRFYEAKIKDLPEVVVWGTGKPLREFLYSDDMARACVFLMKNYDVTGDAKGGEH
VNVGSGIEISIRELAETVKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELKEGIRLAFEDFLRKINALK
>Mature_318_residues
MEKDARIYIAGHKGLVGSAIERVLKKEGYENIIGKSHAELELTEQPKVNEFFENRKPEYVFLAAAKVGGIHANNTYPAEF
IFSNLQIQNNIIDACYRFKVKKLLFLGSSCIYPKFAKQPMDEGQLLDGKLEPTNEPYAIAKIAGIVMCQSYNRQYGTNFI
SVMPTNLYGPGDNYHPENSHVLPALIRRFYEAKIKDLPEVVVWGTGKPLREFLYSDDMARACVFLMKNYDVTGDAKGGEH
VNVGSGIEISIRELAETVKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELKEGIRLAFEDFLRKINALK

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=286, Percent_Identity=34.6153846153846, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI7657641, Length=308, Percent_Identity=24.3506493506494, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1788365, Length=309, Percent_Identity=56.6343042071197, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788353, Length=351, Percent_Identity=24.5014245014245, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994969, Length=360, Percent_Identity=24.1666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=290, Percent_Identity=33.448275862069, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI115532424, Length=226, Percent_Identity=26.1061946902655, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI19922778, Length=292, Percent_Identity=32.1917808219178, Blast_Score=158, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35910; Mature: 35910

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDARIYIAGHKGLVGSAIERVLKKEGYENIIGKSHAELELTEQPKVNEFFENRKPEYV
CCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEECCCCCHHHHHHCCCCCEE
FLAAAKVGGIHANNTYPAEFIFSNLQIQNNIIDACYRFKVKKLLFLGSSCIYPKFAKQPM
EEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC
DEGQLLDGKLEPTNEPYAIAKIAGIVMCQSYNRQYGTNFISVMPTNLYGPGDNYHPENSH
CCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
VLPALIRRFYEAKIKDLPEVVVWGTGKPLREFLYSDDMARACVFLMKNYDVTGDAKGGEH
CHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCE
VNVGSGIEISIRELAETVKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELK
EECCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHH
EGIRLAFEDFLRKINALK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKDARIYIAGHKGLVGSAIERVLKKEGYENIIGKSHAELELTEQPKVNEFFENRKPEYV
CCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEECCCCCHHHHHHCCCCCEE
FLAAAKVGGIHANNTYPAEFIFSNLQIQNNIIDACYRFKVKKLLFLGSSCIYPKFAKQPM
EEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC
DEGQLLDGKLEPTNEPYAIAKIAGIVMCQSYNRQYGTNFISVMPTNLYGPGDNYHPENSH
CCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
VLPALIRRFYEAKIKDLPEVVVWGTGKPLREFLYSDDMARACVFLMKNYDVTGDAKGGEH
CHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCE
VNVGSGIEISIRELAETVKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELK
EECCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHH
EGIRLAFEDFLRKINALK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]