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The map label for this gene is dctB [H]

Identifier: 116327435

GI number: 116327435

Start: 721037

End: 723169

Strand: Direct

Name: dctB [H]

Synonym: LBL_0649

Alternate gene names: 116327435

Gene position: 721037-723169 (Clockwise)

Preceding gene: 116327434

Following gene: 116327438

Centisome position: 19.95

GC content: 43.83

Gene sequence:

>2133_bases
ATGACAAATTCAAAAAATCATTCGCGATTTTCGGCTTTCGATTTTGGGTTCTTGGTATTTTGGATCAGTGTGATTTTGGT
GATCGCGTCTTTTTTCGGAAATTATTATTACGAAGAAAGGCATCTAGACAGGCTTATAGACAATATTCACTGGACCGTTT
CCTATTTATGTGCGGCGGCTCTCGCCTGGCTCGGTTATTTTTCCGCCGAAAGCGGAATTCATCGTTTTAGACTTTGGTTC
GCGCTCGGTCTTACGGCAAACGCGCTCGGACAATTTTCTTGGGCGGTTCAAGTTTATCTTGATTATTACATAATTCCTAC
TCCGAGCGATTATCTTTTTCCCTGGGTGGCTCCCTGTTTTGTCATTGGATACTCTATTATAGTTATTGAATGTGATCGAA
GTCGGATCCGAACGGCTGTGTTGGACGGGTTAGGACTCATCACTGCGATTCTCACTTTTTCTCTTGCGTTGTATCTTCCT
CAAAGGGAGGGGGTCGGAATTTCTCAGCTTCTTCCTTTGATCAATCATCCGGTTTCTTTTTTAACCGCGGCGGCTCTAGG
AGTTTTACTCATTCCTCTTTTAAGATTACAGCCGGATAAGTCCTGGTTTTTTTTCATACTCGGTATGGGAGGAACGGGAC
TTTGTTGGTTGTTATGGAACGCGTTTTTTATCGTGCAGATTCCTCCGGATGGAACCGTGCTCAATGCGGGTTTTTCCGTT
TCCGCTTTAATTTTGGGGTACGAAGCTTTGACTTGGGAACCCAAATTCAACGACTGTCCAGTTTGGGGAAGAAGGTTCGA
AGCGGCGCTTCGTTTATTACCGTTGTTTGAAGTAGTTGCGAGTTCGATTACGATCGTACTTGCGGGAACTCTCAGTGGAT
TGCCCGAAGGAGTTCGAATTATCGCGTGGACTGGAACTACGATCGTGGTTGTGATTGCGAGCGCCAGACAAACCTTTCTC
GTGAAGGAAATGGCGAATGCGGAACAGGAAATCCGTTTGATCAACGAAGGACTTGAGGGGATCGTCGCCAAAAGAACGGA
GGAGTTGAGGACCGTAAATCAATATCTCACTTCCAAAAATGAGCAAGTGATTCGGGCGATCGAAAATTTAAAAAGTACAC
AAAAACAACTGATTCGCTCCGAGAAGATGGCCGTTCTCGGACAGTTGGTCGCCGGAATTGTGCACGAACTCAACACACCA
TTAGGCGCCATTGTTTCTTCGAACGAAGGGATTCGGTCCATATTGTCCAATTCTTGGGAAGGTCTTCTAAGAAGTTATTC
GAGATTTACGGAAGAGGAAAAAACGATTTGGGAAAAACTTTTTTCCAAAGGAATTGCGCTGAGGGAATTTTACGATACCA
GAGAAGAAAGAGTCAAAAGGAAAAAAATCGCAGTTTTGTTAAACGACTTCGGGTTTTCCGAGGTTATGCGTCTCGCGGAT
ATGCTCACTGATTTGGGAATTAGTCCCGACTATGTTTCGGAGAATTTTAAGAATCTGCCTCAAAAAGAAAAACTGATCAT
GATCGTACAGAATGCTCTTTCTCTTTCCGGTCTTGCCCGGGCGAGTTTTACGATCGAGAAGGCGGCGGAAAAGGCCTCCC
TTGTAATACAAGCCTTAAAGGAATATGCGTATAAAGATCCTGCTGAAACGACGATGGGGCCTGTGGATGTACGTAAACAA
TTGGAAACCGTATTTACGCTTTATTATTCGAAATACAAGACTCAAGTGGAGATCGTTCGTGATATGCCTGAAATTTCCTG
CGTTTGGGGAAATTCGGAATCATTGACTCAGGTATGGATTAATTTGATAGGGAATGCACTTTATGCGATGCATTATAAGG
GGAAAATGATAATCTCCTGTAAGAGAGTACAGACCGGTTTGGAAATTGCGATCAAGGATAGTGGAAATGGAATCGAAGAT
ACCATACGAGACCGCATCTTCGAACCGTTTTTCACCACAAAACCTTCCGGCGCGGGAACCGGTCTAGGTTTAGATATATG
TAGAAGAATCATAGAAGATCATAACGGAAAGATCTTTTTTGAAACTTCGGAACAGGGGACCACTTTTTTCGTGATCCTTC
CCGTTGCCGAACCGTCTCCGGAAAAATTAGAAAATTCTACTTTCTTAGTGTGA

Upstream 100 bases:

>100_bases
GCGCTTAAAAAGGAACCTGCTTTGAGAATTTTAAGAACGAGAAAAAGGTCGACGAAATGGGTGGCTAACGTTTAAACTGG
TCGTCGTCTGTGCACGCTTG

Downstream 100 bases:

>100_bases
CCTAAACTTTCTTTTTTATCTCTTCCGTGTTATTTTTATGATTCGTTTTGTTCGGAATGTTTTTTCCTTTGATGAAAGCC
GGGCGTTCTGCGAGTTCTTC

Product: sensor histidine kinase of a two component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MTNSKNHSRFSAFDFGFLVFWISVILVIASFFGNYYYEERHLDRLIDNIHWTVSYLCAAALAWLGYFSAESGIHRFRLWF
ALGLTANALGQFSWAVQVYLDYYIIPTPSDYLFPWVAPCFVIGYSIIVIECDRSRIRTAVLDGLGLITAILTFSLALYLP
QREGVGISQLLPLINHPVSFLTAAALGVLLIPLLRLQPDKSWFFFILGMGGTGLCWLLWNAFFIVQIPPDGTVLNAGFSV
SALILGYEALTWEPKFNDCPVWGRRFEAALRLLPLFEVVASSITIVLAGTLSGLPEGVRIIAWTGTTIVVVIASARQTFL
VKEMANAEQEIRLINEGLEGIVAKRTEELRTVNQYLTSKNEQVIRAIENLKSTQKQLIRSEKMAVLGQLVAGIVHELNTP
LGAIVSSNEGIRSILSNSWEGLLRSYSRFTEEEKTIWEKLFSKGIALREFYDTREERVKRKKIAVLLNDFGFSEVMRLAD
MLTDLGISPDYVSENFKNLPQKEKLIMIVQNALSLSGLARASFTIEKAAEKASLVIQALKEYAYKDPAETTMGPVDVRKQ
LETVFTLYYSKYKTQVEIVRDMPEISCVWGNSESLTQVWINLIGNALYAMHYKGKMIISCKRVQTGLEIAIKDSGNGIED
TIRDRIFEPFFTTKPSGAGTGLGLDICRRIIEDHNGKIFFETSEQGTTFFVILPVAEPSPEKLENSTFLV

Sequences:

>Translated_710_residues
MTNSKNHSRFSAFDFGFLVFWISVILVIASFFGNYYYEERHLDRLIDNIHWTVSYLCAAALAWLGYFSAESGIHRFRLWF
ALGLTANALGQFSWAVQVYLDYYIIPTPSDYLFPWVAPCFVIGYSIIVIECDRSRIRTAVLDGLGLITAILTFSLALYLP
QREGVGISQLLPLINHPVSFLTAAALGVLLIPLLRLQPDKSWFFFILGMGGTGLCWLLWNAFFIVQIPPDGTVLNAGFSV
SALILGYEALTWEPKFNDCPVWGRRFEAALRLLPLFEVVASSITIVLAGTLSGLPEGVRIIAWTGTTIVVVIASARQTFL
VKEMANAEQEIRLINEGLEGIVAKRTEELRTVNQYLTSKNEQVIRAIENLKSTQKQLIRSEKMAVLGQLVAGIVHELNTP
LGAIVSSNEGIRSILSNSWEGLLRSYSRFTEEEKTIWEKLFSKGIALREFYDTREERVKRKKIAVLLNDFGFSEVMRLAD
MLTDLGISPDYVSENFKNLPQKEKLIMIVQNALSLSGLARASFTIEKAAEKASLVIQALKEYAYKDPAETTMGPVDVRKQ
LETVFTLYYSKYKTQVEIVRDMPEISCVWGNSESLTQVWINLIGNALYAMHYKGKMIISCKRVQTGLEIAIKDSGNGIED
TIRDRIFEPFFTTKPSGAGTGLGLDICRRIIEDHNGKIFFETSEQGTTFFVILPVAEPSPEKLENSTFLV
>Mature_709_residues
TNSKNHSRFSAFDFGFLVFWISVILVIASFFGNYYYEERHLDRLIDNIHWTVSYLCAAALAWLGYFSAESGIHRFRLWFA
LGLTANALGQFSWAVQVYLDYYIIPTPSDYLFPWVAPCFVIGYSIIVIECDRSRIRTAVLDGLGLITAILTFSLALYLPQ
REGVGISQLLPLINHPVSFLTAAALGVLLIPLLRLQPDKSWFFFILGMGGTGLCWLLWNAFFIVQIPPDGTVLNAGFSVS
ALILGYEALTWEPKFNDCPVWGRRFEAALRLLPLFEVVASSITIVLAGTLSGLPEGVRIIAWTGTTIVVVIASARQTFLV
KEMANAEQEIRLINEGLEGIVAKRTEELRTVNQYLTSKNEQVIRAIENLKSTQKQLIRSEKMAVLGQLVAGIVHELNTPL
GAIVSSNEGIRSILSNSWEGLLRSYSRFTEEEKTIWEKLFSKGIALREFYDTREERVKRKKIAVLLNDFGFSEVMRLADM
LTDLGISPDYVSENFKNLPQKEKLIMIVQNALSLSGLARASFTIEKAAEKASLVIQALKEYAYKDPAETTMGPVDVRKQL
ETVFTLYYSKYKTQVEIVRDMPEISCVWGNSESLTQVWINLIGNALYAMHYKGKMIISCKRVQTGLEIAIKDSGNGIEDT
IRDRIFEPFFTTKPSGAGTGLGLDICRRIIEDHNGKIFFETSEQGTTFFVILPVAEPSPEKLENSTFLV

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=115, Percent_Identity=40, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788549, Length=137, Percent_Identity=35.7664233576642, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790300, Length=151, Percent_Identity=31.1258278145695, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788393, Length=148, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 79759; Mature: 79628

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSKNHSRFSAFDFGFLVFWISVILVIASFFGNYYYEERHLDRLIDNIHWTVSYLCAAA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAWLGYFSAESGIHRFRLWFALGLTANALGQFSWAVQVYLDYYIIPTPSDYLFPWVAPCF
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHH
VIGYSIIVIECDRSRIRTAVLDGLGLITAILTFSLALYLPQREGVGISQLLPLINHPVSF
HHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHH
LTAAALGVLLIPLLRLQPDKSWFFFILGMGGTGLCWLLWNAFFIVQIPPDGTVLNAGFSV
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCCEEEEEECCCCCEEECCCCH
SALILGYEALTWEPKFNDCPVWGRRFEAALRLLPLFEVVASSITIVLAGTLSGLPEGVRI
HHHHEEHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCCCEE
IAWTGTTIVVVIASARQTFLVKEMANAEQEIRLINEGLEGIVAKRTEELRTVNQYLTSKN
EEEECCEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EQVIRAIENLKSTQKQLIRSEKMAVLGQLVAGIVHELNTPLGAIVSSNEGIRSILSNSWE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
GLLRSYSRFTEEEKTIWEKLFSKGIALREFYDTREERVKRKKIAVLLNDFGFSEVMRLAD
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MLTDLGISPDYVSENFKNLPQKEKLIMIVQNALSLSGLARASFTIEKAAEKASLVIQALK
HHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYAYKDPAETTMGPVDVRKQLETVFTLYYSKYKTQVEIVRDMPEISCVWGNSESLTQVWI
HHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
NLIGNALYAMHYKGKMIISCKRVQTGLEIAIKDSGNGIEDTIRDRIFEPFFTTKPSGAGT
HHHHHHHHHEEECCEEEEEEHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCC
GLGLDICRRIIEDHNGKIFFETSEQGTTFFVILPVAEPSPEKLENSTFLV
CCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCHHHHCCCCCCC
>Mature Secondary Structure 
TNSKNHSRFSAFDFGFLVFWISVILVIASFFGNYYYEERHLDRLIDNIHWTVSYLCAAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAWLGYFSAESGIHRFRLWFALGLTANALGQFSWAVQVYLDYYIIPTPSDYLFPWVAPCF
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHH
VIGYSIIVIECDRSRIRTAVLDGLGLITAILTFSLALYLPQREGVGISQLLPLINHPVSF
HHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHH
LTAAALGVLLIPLLRLQPDKSWFFFILGMGGTGLCWLLWNAFFIVQIPPDGTVLNAGFSV
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCCEEEEEECCCCCEEECCCCH
SALILGYEALTWEPKFNDCPVWGRRFEAALRLLPLFEVVASSITIVLAGTLSGLPEGVRI
HHHHEEHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCCCEE
IAWTGTTIVVVIASARQTFLVKEMANAEQEIRLINEGLEGIVAKRTEELRTVNQYLTSKN
EEEECCEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EQVIRAIENLKSTQKQLIRSEKMAVLGQLVAGIVHELNTPLGAIVSSNEGIRSILSNSWE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
GLLRSYSRFTEEEKTIWEKLFSKGIALREFYDTREERVKRKKIAVLLNDFGFSEVMRLAD
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MLTDLGISPDYVSENFKNLPQKEKLIMIVQNALSLSGLARASFTIEKAAEKASLVIQALK
HHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYAYKDPAETTMGPVDVRKQLETVFTLYYSKYKTQVEIVRDMPEISCVWGNSESLTQVWI
HHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
NLIGNALYAMHYKGKMIISCKRVQTGLEIAIKDSGNGIEDTIRDRIFEPFFTTKPSGAGT
HHHHHHHHHEEECCEEEEEEHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCC
GLGLDICRRIIEDHNGKIFFETSEQGTTFFVILPVAEPSPEKLENSTFLV
CCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]