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The map label for this gene is yafJ [H]

Identifier: 116327354

GI number: 116327354

Start: 608359

End: 609141

Strand: Direct

Name: yafJ [H]

Synonym: LBL_0550

Alternate gene names: 116327354

Gene position: 608359-609141 (Clockwise)

Preceding gene: 116327347

Following gene: 116327355

Centisome position: 16.83

GC content: 44.83

Gene sequence:

>783_bases
ATGTGTGAACTTCTTGGTATGAGCGCGAATGTCCCGACAGATATTTGTTTCAGTCTGACCGGTCTTGTACAAAGAGGAGG
AAAAACGGGGCCTCATAAGGATGGATGGGGGATTGCGTTCTATGAAGGGAAGGGACTTCGCATTTTTCACGATCCATCGC
CGGGTGTAGAATCTAAAATTGCCGCCTTCGTTCAAAACCTTCCTATTAAAAGTGAAATTGTGATCAGCCATATCCGTAAA
GCAAACCGCGGAAAAGTGGACTTGAAAAATACGCATCCATTTGTTCGGGAATTTTGGGGCTCTCACTGGACTTTTGCTCA
TAATGGACAGTTCAAAAAAATCAAGGAGAAGTCCCTCAGCCTCGGTGATTTTCAACCGGTTGGAAGTACGGATTCGGAAT
ATGCGTTTTGTTGGCTTTTGGGAAAGTTGAAGAAGAAGTTCAAAAATCCTCCTAAGAAAGAAGCCGAACTTTTCAAAACC
GTTGAGTCTTTGATGCGGGAACTACATCAAAAAGGGGTTTCCAACATTCTCTTCAGCGATTCCAAAAATCTCTATACGTA
CTGTTCCACAAAACTTTCCTGGATCACCCGAAAATCTCCCTTTGGAAAGGCCCGACTTGTCGACGCGGATCTCAGCGTGG
ATTTTCGAAAAGTCACAACTCCGGGAGATAGGGTCACCGTGATCGCAACTCGGCCTCTTACTTCTAATGAAGAATGGACG
ATCTGTCAGCCGGGTGAGTTTTCCGTTTGGAGAAAAGGTCGAATCGTATTTTCCAATCGGTAA

Upstream 100 bases:

>100_bases
GTCATTTCTGCAAGGAATCCTTAAAAAATCACTGGACAGAAAAACTCTGTTTTTGCGAAAATTGTTCTAATATATTAAAC
GGCACTCAAGGAAAAGTGGA

Downstream 100 bases:

>100_bases
GAACAAGTTCTGCACTGAAACGCTTTCGTAAAAAGTAGAGTTGTTGAAGAATTAATTCTCTATCCGTTTCTGCCGCATTA
AAATGGATGTTTGAAGCGGT

Product: glutamine amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHKDGWGIAFYEGKGLRIFHDPSPGVESKIAAFVQNLPIKSEIVISHIRK
ANRGKVDLKNTHPFVREFWGSHWTFAHNGQFKKIKEKSLSLGDFQPVGSTDSEYAFCWLLGKLKKKFKNPPKKEAELFKT
VESLMRELHQKGVSNILFSDSKNLYTYCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATRPLTSNEEWT
ICQPGEFSVWRKGRIVFSNR

Sequences:

>Translated_260_residues
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHKDGWGIAFYEGKGLRIFHDPSPGVESKIAAFVQNLPIKSEIVISHIRK
ANRGKVDLKNTHPFVREFWGSHWTFAHNGQFKKIKEKSLSLGDFQPVGSTDSEYAFCWLLGKLKKKFKNPPKKEAELFKT
VESLMRELHQKGVSNILFSDSKNLYTYCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATRPLTSNEEWT
ICQPGEFSVWRKGRIVFSNR
>Mature_260_residues
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHKDGWGIAFYEGKGLRIFHDPSPGVESKIAAFVQNLPIKSEIVISHIRK
ANRGKVDLKNTHPFVREFWGSHWTFAHNGQFKKIKEKSLSLGDFQPVGSTDSEYAFCWLLGKLKKKFKNPPKKEAELFKT
VESLMRELHQKGVSNILFSDSKNLYTYCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATRPLTSNEEWT
ICQPGEFSVWRKGRIVFSNR

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=256, Percent_Identity=61.328125, Blast_Score=327, Evalue=4e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29398; Mature: 29398

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHKDGWGIAFYEGKGLRIFHDPSPGVESKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHH
AAFVQNLPIKSEIVISHIRKANRGKVDLKNTHPFVREFWGSHWTFAHNGQFKKIKEKSLS
HHHHHCCCCCHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCEEEECCCHHHHHHHCCC
LGDFQPVGSTDSEYAFCWLLGKLKKKFKNPPKKEAELFKTVESLMRELHQKGVSNILFSD
CCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEECC
SKNLYTYCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATRPLTSNEEWT
CCCEEEEECCHHHEEECCCCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCCCCCEE
ICQPGEFSVWRKGRIVFSNR
EECCCCCCEEECCEEEECCC
>Mature Secondary Structure
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHKDGWGIAFYEGKGLRIFHDPSPGVESKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHH
AAFVQNLPIKSEIVISHIRKANRGKVDLKNTHPFVREFWGSHWTFAHNGQFKKIKEKSLS
HHHHHCCCCCHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCEEEECCCHHHHHHHCCC
LGDFQPVGSTDSEYAFCWLLGKLKKKFKNPPKKEAELFKTVESLMRELHQKGVSNILFSD
CCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEECC
SKNLYTYCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATRPLTSNEEWT
CCCEEEEECCHHHEEECCCCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCCCCCEE
ICQPGEFSVWRKGRIVFSNR
EECCCCCCEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]