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The map label for this gene is ilvE [H]

Identifier: 114800499

GI number: 114800499

Start: 3268335

End: 3269219

Strand: Reverse

Name: ilvE [H]

Synonym: HNE_3091

Alternate gene names: 114800499

Gene position: 3269219-3268335 (Counterclockwise)

Preceding gene: 114798185

Following gene: 114800281

Centisome position: 88.24

GC content: 61.02

Gene sequence:

>885_bases
ATGTCTGCCATCCCCTATGATGACCGCGACGGATACATCTGGATGGATGGCTCGTTCGTACCCTGGCGTGACACCAAAGT
GCACGTTCTGACGCATGCATTGCACTACGCTTCCTCCGTTTTTGAAGGCGAGCGCGCCTATAGCGGCAAGATCTTCCGCT
CGCTGGACCACTCCAAGCGGCTGCACAATTCGGCCGGTATCATGGGCTTCGACATTCCCTTCAGCGTGGAGGAAATCGAG
CGGGCCAAGAAAGAGGCGCTGGAAAAATCCGGTCTCGAGAGCGCTTATGTGCGCGCCGTGGCCTGGCGCGGATCGGAAAT
GATGGGCGTTTCGGCGCAGAACAACACCATCCATCTGGCAATCGCTGTCTGGCATTGGGGCGACTATTTTGCCGACAAGA
TGAAGGGCATCCGCCTGACGCATGCCCAGTGGCGCCGCCCGGCGCCCGATACGGCGCCCTGCCATGCCAAGGCGGCGGGC
CTCTATATGATCTGCACCCTCTCCAAGCATGCCGCGGAGAAGGCGGGCTATCAGGACGCGCTGATGCTGGACTATCGCGG
CCAGGTGGCCGAGGCGACCGGCGCGAACATCTTCTTCGTGCGTGACGGCGTTCTGCATACGCCGACCCCGGACTGTTTCC
TCAACGGTCTTACCCGCCAGACGACGATTGCGCTCGCCAAGGCGCGCCAGATCGAAGTGGTCGAACGGGCGATCTTCCCG
GATGAGCTTTCAACGTTCAGCGAATGCTTCATCACGGGCTCTGCTGCGGAGCTGACGCCGGTCGCCGAGATCGGCGAGCA
TCGCTACAAGCCTGGCCAGATCTCCGAAGCGCTGGTGAACGACTATTCCAACCTCTGTAACGGGAAGCTGGAGCTGGCGC
TTTAG

Upstream 100 bases:

>100_bases
AACAGGCGGGGGTCGAATGGGCGGTTCAGGTTGACGGATTGGGCCATAAGTCGGAAATGAACGCGAACCGCAACAAAAGT
GCAAGCGAGGAGCGTAAGCA

Downstream 100 bases:

>100_bases
GGCGTCAGTAGGGCGGACTGACAAACACTTTCAGTTCTTGTTTGTCTGGGTGGAGCGTGACAATCGCCTGATTGTTGTCG
ACGCTGATCCCGAGGTTTCC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSAIPYDDRDGYIWMDGSFVPWRDTKVHVLTHALHYASSVFEGERAYSGKIFRSLDHSKRLHNSAGIMGFDIPFSVEEIE
RAKKEALEKSGLESAYVRAVAWRGSEMMGVSAQNNTIHLAIAVWHWGDYFADKMKGIRLTHAQWRRPAPDTAPCHAKAAG
LYMICTLSKHAAEKAGYQDALMLDYRGQVAEATGANIFFVRDGVLHTPTPDCFLNGLTRQTTIALAKARQIEVVERAIFP
DELSTFSECFITGSAAELTPVAEIGEHRYKPGQISEALVNDYSNLCNGKLELAL

Sequences:

>Translated_294_residues
MSAIPYDDRDGYIWMDGSFVPWRDTKVHVLTHALHYASSVFEGERAYSGKIFRSLDHSKRLHNSAGIMGFDIPFSVEEIE
RAKKEALEKSGLESAYVRAVAWRGSEMMGVSAQNNTIHLAIAVWHWGDYFADKMKGIRLTHAQWRRPAPDTAPCHAKAAG
LYMICTLSKHAAEKAGYQDALMLDYRGQVAEATGANIFFVRDGVLHTPTPDCFLNGLTRQTTIALAKARQIEVVERAIFP
DELSTFSECFITGSAAELTPVAEIGEHRYKPGQISEALVNDYSNLCNGKLELAL
>Mature_293_residues
SAIPYDDRDGYIWMDGSFVPWRDTKVHVLTHALHYASSVFEGERAYSGKIFRSLDHSKRLHNSAGIMGFDIPFSVEEIER
AKKEALEKSGLESAYVRAVAWRGSEMMGVSAQNNTIHLAIAVWHWGDYFADKMKGIRLTHAQWRRPAPDTAPCHAKAAGL
YMICTLSKHAAEKAGYQDALMLDYRGQVAEATGANIFFVRDGVLHTPTPDCFLNGLTRQTTIALAKARQIEVVERAIFPD
ELSTFSECFITGSAAELTPVAEIGEHRYKPGQISEALVNDYSNLCNGKLELAL

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=293, Percent_Identity=38.2252559726962, Blast_Score=192, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6322002, Length=296, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32699; Mature: 32568

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIPYDDRDGYIWMDGSFVPWRDTKVHVLTHALHYASSVFEGERAYSGKIFRSLDHSKR
CCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHH
LHNSAGIMGFDIPFSVEEIERAKKEALEKSGLESAYVRAVAWRGSEMMGVSAQNNTIHLA
HHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCEEEEECCCCEEEEE
IAVWHWGDYFADKMKGIRLTHAQWRRPAPDTAPCHAKAAGLYMICTLSKHAAEKAGYQDA
EEEEECCHHHHHHHCCEEEEHHHCCCCCCCCCCCCHHCCCEEEEEECCHHHHHHCCCCCE
LMLDYRGQVAEATGANIFFVRDGVLHTPTPDCFLNGLTRQTTIALAKARQIEVVERAIFP
EEEECCCCEECCCCCEEEEEECCEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCH
DELSTFSECFITGSAAELTPVAEIGEHRYKPGQISEALVNDYSNLCNGKLELAL
HHHHHHHHHEECCCCHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
SAIPYDDRDGYIWMDGSFVPWRDTKVHVLTHALHYASSVFEGERAYSGKIFRSLDHSKR
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHH
LHNSAGIMGFDIPFSVEEIERAKKEALEKSGLESAYVRAVAWRGSEMMGVSAQNNTIHLA
HHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCEEEEECCCCEEEEE
IAVWHWGDYFADKMKGIRLTHAQWRRPAPDTAPCHAKAAGLYMICTLSKHAAEKAGYQDA
EEEEECCHHHHHHHCCEEEEHHHCCCCCCCCCCCCHHCCCEEEEEECCHHHHHHCCCCCE
LMLDYRGQVAEATGANIFFVRDGVLHTPTPDCFLNGLTRQTTIALAKARQIEVVERAIFP
EEEECCCCEECCCCCEEEEEECCEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCH
DELSTFSECFITGSAAELTPVAEIGEHRYKPGQISEALVNDYSNLCNGKLELAL
HHHHHHHHHEECCCCHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]