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Identifier: 114800420

GI number: 114800420

Start: 505844

End: 506374

Strand: Direct

Name: def

Synonym: HNE_0512

Alternate gene names: 114800420

Gene position: 505844-506374 (Clockwise)

Preceding gene: 114799682

Following gene: 114799265

Centisome position: 13.65

GC content: 58.38

Gene sequence:

>531_bases
ATGGCTATACGCGAAATCCTCACCGTCCCCGATCCGCGCCTGAAACAGGTGTCCAAGCCCGTTGAGGGCGGGGTTACCGA
TGACATCCGCGCGCTCATGGATGACATGCTGGAGACCATGTATGACGCGCCCGGCATTGGCCTGGCCGCGATCCAGATCG
GCGTGCCGCTGCGTGTGATCGTCATGGATCTGGCCCGCGAAGGCGAAGAGCCTGCGCCGCGCTATTTCGTGAACCCGGAA
ATCCTCGAAACCATTGAGGAAAAGAAGCCTTATGAAGAAGGCTGCCTTTCAGTTCCGGACATTTTCGACCAGGTGGAGCG
CTCTGCCCGGTGCAGAATCCGTTATCTGGACTATGATGGCAAGCAAGTGGATGAGTGGGCAGAAGACCTCTATGCTGTCT
GCATCCAGCACGAGATGGATCACCTCGAAGGCACATTGTTCATCGACTACCTCTCCCGCCTGAAGCGCGACCGCGCCATC
GACAAGGTGAAGAAGGCCAAGATCCGCGCCATCCGCGAAGACGCCAACTGA

Upstream 100 bases:

>100_bases
TACGCCCGATACCGGCCAACTGCCGCTGGAGCCGCCCAGGAAGCTTCCCGCAGAGTGATCGCCCGTTGACGCTGGCGGAT
GGGGTGCATATCTGACGCTT

Downstream 100 bases:

>100_bases
TCGGCCACTGCCGTCTCGACCCGCAGGCGGCGAGAGACTATGAGGCTGGCCTATGCCAGACCAAATAACCCCGCCTCTCC
GCATTGCCTTCATGGGCAGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MAIREILTVPDPRLKQVSKPVEGGVTDDIRALMDDMLETMYDAPGIGLAAIQIGVPLRVIVMDLAREGEEPAPRYFVNPE
ILETIEEKKPYEEGCLSVPDIFDQVERSARCRIRYLDYDGKQVDEWAEDLYAVCIQHEMDHLEGTLFIDYLSRLKRDRAI
DKVKKAKIRAIREDAN

Sequences:

>Translated_176_residues
MAIREILTVPDPRLKQVSKPVEGGVTDDIRALMDDMLETMYDAPGIGLAAIQIGVPLRVIVMDLAREGEEPAPRYFVNPE
ILETIEEKKPYEEGCLSVPDIFDQVERSARCRIRYLDYDGKQVDEWAEDLYAVCIQHEMDHLEGTLFIDYLSRLKRDRAI
DKVKKAKIRAIREDAN
>Mature_175_residues
AIREILTVPDPRLKQVSKPVEGGVTDDIRALMDDMLETMYDAPGIGLAAIQIGVPLRVIVMDLAREGEEPAPRYFVNPEI
LETIEEKKPYEEGCLSVPDIFDQVERSARCRIRYLDYDGKQVDEWAEDLYAVCIQHEMDHLEGTLFIDYLSRLKRDRAID
KVKKAKIRAIREDAN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=165, Percent_Identity=35.1515151515151, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=47.8787878787879, Blast_Score=142, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645726, Length=161, Percent_Identity=32.9192546583851, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=30.9090909090909, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_HYPNA (Q0C4V1)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759242.1
- ProteinModelPortal:   Q0C4V1
- SMR:   Q0C4V1
- STRING:   Q0C4V1
- GeneID:   4290364
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0512
- NMPDR:   fig|228405.5.peg.504
- TIGR:   HNE_0512
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- PhylomeDB:   Q0C4V1
- ProtClustDB:   CLSK2532150
- BioCyc:   HNEP81032:HNE_0512-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20178; Mature: 20047

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIREILTVPDPRLKQVSKPVEGGVTDDIRALMDDMLETMYDAPGIGLAAIQIGVPLRVI
CCHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHH
VMDLAREGEEPAPRYFVNPEILETIEEKKPYEEGCLSVPDIFDQVERSARCRIRYLDYDG
HHHHHHCCCCCCCCEECCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCEEEEECCCC
KQVDEWAEDLYAVCIQHEMDHLEGTLFIDYLSRLKRDRAIDKVKKAKIRAIREDAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIREILTVPDPRLKQVSKPVEGGVTDDIRALMDDMLETMYDAPGIGLAAIQIGVPLRVI
CHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHH
VMDLAREGEEPAPRYFVNPEILETIEEKKPYEEGCLSVPDIFDQVERSARCRIRYLDYDG
HHHHHHCCCCCCCCEECCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCEEEEECCCC
KQVDEWAEDLYAVCIQHEMDHLEGTLFIDYLSRLKRDRAIDKVKKAKIRAIREDAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA