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The map label for this gene is ruvB

Identifier: 114800414

GI number: 114800414

Start: 130029

End: 131063

Strand: Direct

Name: ruvB

Synonym: HNE_0147

Alternate gene names: 114800414

Gene position: 130029-131063 (Clockwise)

Preceding gene: 114798884

Following gene: 114797615

Centisome position: 3.51

GC content: 66.47

Gene sequence:

>1035_bases
ATGAGCCGCGAGGGCGCCCTCTCCGATCCCAACGCCCAAGGCGGGGATGCGCTCGACCGCGCCCTCCGCCCACAGACGTT
TGAGGATTATGTTGGCCAACGCAAGGCAAAGGCCAATCTCAAGGTTTATGTCGAGGCGGCGCGCGGGCGCAAGGAAGCGC
TCGACCATGTGTTGCTGTTCGGCCCGCCCGGCCTGGGAAAGACCACGCTGGCGCAGATCCTCGCGCGGGAGATGGGCGTG
GGTTTCCGCGCCACCTCCGGGCCGGTGATTGCCAAGGCGGGCGATCTTGCTGCCATCCTTACGAACCTTGAGCCCAATGA
CGTGCTGTTCATTGACGAGATCCACCGCCTGCCGGCCATCGTGGAAGAGATCCTCTATCCCGCGATGGAGGATTACGCGC
TCGACCTGGTGATCGGGGAGGGGCCAGCAGCGCGCTCCGTGCGGCTCGATCTCGCGCCGTTCACACTGGTGGGCGCCACC
ACGCGCGCCGGCCTTCTCGCAACGCCGCTGCGGGACCGGTTCGGCATCCCCGTTCGTTTGGAATTCTACGAACCCGAAGA
GCTTGGCCGCATCGTCATGGCGGCGGCCCGCAAGCTCGGTGTTCCGATCACGGAAGATGGTGCGGTGGAGATTTCCGCCC
GCGCGCGCGGTACGCCGCGTATCGCGCTGCGCCTCTTGCGCCGGGTGCGCGACTTTGCCGAGGCCGAAGGGGCCGACATC
AACCGCGCCTCAGCCGCCCGCGCGCTCAAGCGGCTCGAGATTGACGAAGATGGGCTCGACGCGCTCGACCGGCGGTATCT
GCATGCGCTGGTGAAAACCTATGCGGGCGGGCCAGTGGGCGCCGATACGCTCGCGGCGGCCTTGTCCGAAGCGCGCGACG
CGGTGGAAGATGTGATCGAGCCTTACCTGATGCAGAAAGGCTATGTGGCACGCACGCCGCGCGGGCGGGTTGCTGCGCCG
CTCGCCTATGAGCGGTTGGGGCTGAAGGCGCCGAATGGCCAGCAATCTGCCCTTTTTGGTGAAGACGAAGACTGA

Upstream 100 bases:

>100_bases
ATTCGCAGAGCGAGGCCGCCAGGGCTGTGGCGGCGGCGGCCAAGAACGGGGCTGAAGAAGTTGGCGCGCTGGTCAAAGGC
GCGCTGAAGGAACTGGCCAG

Downstream 100 bases:

>100_bases
TCAGCCCGCATCATCTGTCCCGGCTGCTCCGCCGCCCATCGCCCACAGAAGGCCGATAATTGCGCCCTTGATCCGGGGAA
GGAGCATCAGTACTGCCAGG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSREGALSDPNAQGGDALDRALRPQTFEDYVGQRKAKANLKVYVEAARGRKEALDHVLLFGPPGLGKTTLAQILAREMGV
GFRATSGPVIAKAGDLAAILTNLEPNDVLFIDEIHRLPAIVEEILYPAMEDYALDLVIGEGPAARSVRLDLAPFTLVGAT
TRAGLLATPLRDRFGIPVRLEFYEPEELGRIVMAAARKLGVPITEDGAVEISARARGTPRIALRLLRRVRDFAEAEGADI
NRASAARALKRLEIDEDGLDALDRRYLHALVKTYAGGPVGADTLAAALSEARDAVEDVIEPYLMQKGYVARTPRGRVAAP
LAYERLGLKAPNGQQSALFGEDED

Sequences:

>Translated_344_residues
MSREGALSDPNAQGGDALDRALRPQTFEDYVGQRKAKANLKVYVEAARGRKEALDHVLLFGPPGLGKTTLAQILAREMGV
GFRATSGPVIAKAGDLAAILTNLEPNDVLFIDEIHRLPAIVEEILYPAMEDYALDLVIGEGPAARSVRLDLAPFTLVGAT
TRAGLLATPLRDRFGIPVRLEFYEPEELGRIVMAAARKLGVPITEDGAVEISARARGTPRIALRLLRRVRDFAEAEGADI
NRASAARALKRLEIDEDGLDALDRRYLHALVKTYAGGPVGADTLAAALSEARDAVEDVIEPYLMQKGYVARTPRGRVAAP
LAYERLGLKAPNGQQSALFGEDED
>Mature_343_residues
SREGALSDPNAQGGDALDRALRPQTFEDYVGQRKAKANLKVYVEAARGRKEALDHVLLFGPPGLGKTTLAQILAREMGVG
FRATSGPVIAKAGDLAAILTNLEPNDVLFIDEIHRLPAIVEEILYPAMEDYALDLVIGEGPAARSVRLDLAPFTLVGATT
RAGLLATPLRDRFGIPVRLEFYEPEELGRIVMAAARKLGVPITEDGAVEISARARGTPRIALRLLRRVRDFAEAEGADIN
RASAARALKRLEIDEDGLDALDRRYLHALVKTYAGGPVGADTLAAALSEARDAVEDVIEPYLMQKGYVARTPRGRVAAPL
AYERLGLKAPNGQQSALFGEDED

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=317, Percent_Identity=59.9369085173502, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_HYPNA (Q0C5W2)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758881.1
- ProteinModelPortal:   Q0C5W2
- SMR:   Q0C5W2
- STRING:   Q0C5W2
- GeneID:   4290358
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0147
- NMPDR:   fig|228405.5.peg.145
- TIGR:   HNE_0147
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   Q0C5W2
- ProtClustDB:   PRK00080
- BioCyc:   HNEP81032:HNE_0147-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37111; Mature: 36980

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREGALSDPNAQGGDALDRALRPQTFEDYVGQRKAKANLKVYVEAARGRKEALDHVLLF
CCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHCEEEEE
GPPGLGKTTLAQILAREMGVGFRATSGPVIAKAGDLAAILTNLEPNDVLFIDEIHRLPAI
CCCCCCHHHHHHHHHHHHCCCEEECCCCEEEECCCHHHHHHCCCCCCEEEHHHHHHHHHH
VEEILYPAMEDYALDLVIGEGPAARSVRLDLAPFTLVGATTRAGLLATPLRDRFGIPVRL
HHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCEEEECCCHHCCHHCCCHHHHCCCCEEE
EFYEPEELGRIVMAAARKLGVPITEDGAVEISARARGTPRIALRLLRRVRDFAEAEGADI
EEECHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
NRASAARALKRLEIDEDGLDALDRRYLHALVKTYAGGPVGADTLAAALSEARDAVEDVIE
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
PYLMQKGYVARTPRGRVAAPLAYERLGLKAPNGQQSALFGEDED
HHHHCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SREGALSDPNAQGGDALDRALRPQTFEDYVGQRKAKANLKVYVEAARGRKEALDHVLLF
CCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHCEEEEE
GPPGLGKTTLAQILAREMGVGFRATSGPVIAKAGDLAAILTNLEPNDVLFIDEIHRLPAI
CCCCCCHHHHHHHHHHHHCCCEEECCCCEEEECCCHHHHHHCCCCCCEEEHHHHHHHHHH
VEEILYPAMEDYALDLVIGEGPAARSVRLDLAPFTLVGATTRAGLLATPLRDRFGIPVRL
HHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCEEEECCCHHCCHHCCCHHHHCCCCEEE
EFYEPEELGRIVMAAARKLGVPITEDGAVEISARARGTPRIALRLLRRVRDFAEAEGADI
EEECHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
NRASAARALKRLEIDEDGLDALDRRYLHALVKTYAGGPVGADTLAAALSEARDAVEDVIE
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
PYLMQKGYVARTPRGRVAAPLAYERLGLKAPNGQQSALFGEDED
HHHHCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA