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The map label for this gene is hemB [H]

Identifier: 114800380

GI number: 114800380

Start: 916471

End: 917466

Strand: Reverse

Name: hemB [H]

Synonym: HNE_0898

Alternate gene names: 114800380

Gene position: 917466-916471 (Counterclockwise)

Preceding gene: 114799089

Following gene: 114799250

Centisome position: 24.76

GC content: 65.16

Gene sequence:

>996_bases
ATGACCCAGTTTCCACAGTCCTTTCCCGGAACCCGTATGCGCCGCCTGCGCCAGGCCCCGTGGGCGCGCAGCCTTGTGGC
AGAGCATGTTCTGACCCCGGCAGACCTTGTCTGGGCGGTCTTCGTGCATGATGGCGAGGGGCGCATTCCGGTCGCCTCCC
TGCCCGGCGTGGACCGGCTCAGCGTGGCAGAGGCCGCGCTGGCCGCCAAGCGCGCCAGGGCGCTCGGCATTCCGGCGATT
GCGCTGTTTCCCTATATCGGGCCCGAGGGCAAGGACGACGAAGGCAGCGGCGCGCTTTCCCCCGATGGCCTCGTGCCGCG
CGCGCTGAAAGCCATGAAGGACGCTGCGCCGGAAGTGGGCCTGATCACCGATGTGGCACTTGATGCGTATACGTCGCATG
GGCATGACGGGCTGCTGGACGAGGATGGCACCATCCCGAACGACGCAACCGTTGAGCGGCTTGTCGAACAGGCGCTGGTG
CATGTGGGCGCGGGCGCGGATGTCGTGGCGCCGTCTGACATGATGGACGGGCGCATTGGCGCCATCCGCTATGCCTTCGA
GGAAGAAGGCTTCACGAATGCGATGATCCTGGCCTATGCGGCGAAGTATGCGAGCGGGTTTTATGGGCCGTATCGCGAGG
CCATTGGCTCGGCCGCCGCGCTCAAAGGCGACAAGAAAACCTATCAGCAGGACCCGGCCAATTCGGACGAGGCGCTGCGC
GAGGTCGCGCTCGATCTCGCCGACGGCGCGGACATGGTGATGGTGAAACCGGGTCTTCCGTATCTGGACATTGTTCACCG
GGTTTCGACGACGTTCGGCGTGCCGACGCTGGCCTATCAGGTTTCCGGTGAGTACGCGCAGATCATGGCGGCGGCCCAGA
ATGGCTGGATCGACGGCGACCGCGTGATGATGGAAACGCTGATGAGCTTCAAGCGCGCGGGCGCAGCTGGCGTAATCACG
TATTTTGCGGAACGCGCGGCGGAGAAGCTGGGCTGA

Upstream 100 bases:

>100_bases
GCCCAGGACGAGAACGGTCTGGATGTGCGTCAGCGCTGCTTCATGGCGCGGCGATTGGGCTGGTTGTGACGGCGCCGGAT
TTGGCGGTAGGAGTTAAGGT

Downstream 100 bases:

>100_bases
AAATCAGCCCAGCTTTCATTCGGTCTCGTTACGCCGCTTCTTTGATCGCCACCAGTTCAATGTCGAACGTCAGGTCCTGA
CCGGCGAGCGGGTGATTGGC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTQFPQSFPGTRMRRLRQAPWARSLVAEHVLTPADLVWAVFVHDGEGRIPVASLPGVDRLSVAEAALAAKRARALGIPAI
ALFPYIGPEGKDDEGSGALSPDGLVPRALKAMKDAAPEVGLITDVALDAYTSHGHDGLLDEDGTIPNDATVERLVEQALV
HVGAGADVVAPSDMMDGRIGAIRYAFEEEGFTNAMILAYAAKYASGFYGPYREAIGSAAALKGDKKTYQQDPANSDEALR
EVALDLADGADMVMVKPGLPYLDIVHRVSTTFGVPTLAYQVSGEYAQIMAAAQNGWIDGDRVMMETLMSFKRAGAAGVIT
YFAERAAEKLG

Sequences:

>Translated_331_residues
MTQFPQSFPGTRMRRLRQAPWARSLVAEHVLTPADLVWAVFVHDGEGRIPVASLPGVDRLSVAEAALAAKRARALGIPAI
ALFPYIGPEGKDDEGSGALSPDGLVPRALKAMKDAAPEVGLITDVALDAYTSHGHDGLLDEDGTIPNDATVERLVEQALV
HVGAGADVVAPSDMMDGRIGAIRYAFEEEGFTNAMILAYAAKYASGFYGPYREAIGSAAALKGDKKTYQQDPANSDEALR
EVALDLADGADMVMVKPGLPYLDIVHRVSTTFGVPTLAYQVSGEYAQIMAAAQNGWIDGDRVMMETLMSFKRAGAAGVIT
YFAERAAEKLG
>Mature_330_residues
TQFPQSFPGTRMRRLRQAPWARSLVAEHVLTPADLVWAVFVHDGEGRIPVASLPGVDRLSVAEAALAAKRARALGIPAIA
LFPYIGPEGKDDEGSGALSPDGLVPRALKAMKDAAPEVGLITDVALDAYTSHGHDGLLDEDGTIPNDATVERLVEQALVH
VGAGADVVAPSDMMDGRIGAIRYAFEEEGFTNAMILAYAAKYASGFYGPYREAIGSAAALKGDKKTYQQDPANSDEALRE
VALDLADGADMVMVKPGLPYLDIVHRVSTTFGVPTLAYQVSGEYAQIMAAAQNGWIDGDRVMMETLMSFKRAGAAGVITY
FAERAAEKLG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=39.6103896103896, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI87081728, Length=319, Percent_Identity=45.7680250783699, Blast_Score=240, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321398, Length=309, Percent_Identity=39.4822006472492, Blast_Score=195, Evalue=7e-51,
Organism=Drosophila melanogaster, GI21358291, Length=294, Percent_Identity=38.7755102040816, Blast_Score=211, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35195; Mature: 35064

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFPQSFPGTRMRRLRQAPWARSLVAEHVLTPADLVWAVFVHDGEGRIPVASLPGVDRL
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHEEEEEEECCCCCEEEECCCCCCHH
SVAEAALAAKRARALGIPAIALFPYIGPEGKDDEGSGALSPDGLVPRALKAMKDAAPEVG
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
LITDVALDAYTSHGHDGLLDEDGTIPNDATVERLVEQALVHVGAGADVVAPSDMMDGRIG
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHH
AIRYAFEEEGFTNAMILAYAAKYASGFYGPYREAIGSAAALKGDKKTYQQDPANSDEALR
HEEEEHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHHHCCCHHHHHCCCCCHHHHHH
EVALDLADGADMVMVKPGLPYLDIVHRVSTTFGVPTLAYQVSGEYAQIMAAAQNGWIDGD
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCHH
RVMMETLMSFKRAGAAGVITYFAERAAEKLG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQFPQSFPGTRMRRLRQAPWARSLVAEHVLTPADLVWAVFVHDGEGRIPVASLPGVDRL
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHEEEEEEECCCCCEEEECCCCCCHH
SVAEAALAAKRARALGIPAIALFPYIGPEGKDDEGSGALSPDGLVPRALKAMKDAAPEVG
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
LITDVALDAYTSHGHDGLLDEDGTIPNDATVERLVEQALVHVGAGADVVAPSDMMDGRIG
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHH
AIRYAFEEEGFTNAMILAYAAKYASGFYGPYREAIGSAAALKGDKKTYQQDPANSDEALR
HEEEEHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHHHCCCHHHHHCCCCCHHHHHH
EVALDLADGADMVMVKPGLPYLDIVHRVSTTFGVPTLAYQVSGEYAQIMAAAQNGWIDGD
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCHH
RVMMETLMSFKRAGAAGVITYFAERAAEKLG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]