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The map label for this gene is aes [H]

Identifier: 114800239

GI number: 114800239

Start: 3126373

End: 3127338

Strand: Reverse

Name: aes [H]

Synonym: HNE_2970

Alternate gene names: 114800239

Gene position: 3127338-3126373 (Counterclockwise)

Preceding gene: 114799877

Following gene: 114797434

Centisome position: 84.41

GC content: 65.94

Gene sequence:

>966_bases
ATGACATCACGCCCCCTCGTCGATCCCGAACTGATCGCCTTCATAGAGGCCTTCCCGACTGTGAACATCGGGGCGGAAAA
TCTCGCAGCGGTTCGCCAGAGCCGGGACGCAGCCCTTGCCCAGTTGCCCCCCTTTCCGGCCAGTGTCCGCGTGGAGGAGG
TGTTCATCCCCGGCCCCAAAGGCGCGCCCGATGTGCGGTTAAAGCTCTACCGCAAGAAGGACGGTGCAGAGGGGCCGCGC
CCGGCGATCCTCCACCTGCATGGCGGGGGCTATATCATGGGCAATCCGGAATTCATGGGGCCGATGTGCGCGCTCTGGGC
AGACGCCTATGACGCGGTTGTGGTGGCGCCCGCATATCGTATCGCGCCGGAGACGGCCTTTCCCGGCAATGTGGAAGACG
CCTACGCTGCGCTGGCCTGGGCGAACGCCCGCGCGGCCGATCTGGGTATCGATACCGCCCGCATCGCCGTTGCCGGGGAG
AGCGCGGGCGGAGGCCTCGCCGCGTCGCTCGCCCTTCTGGTGCGCGACCGGGGCGAATACAGTTTCTGCCACCAGCAGCT
GATCTTCCCGATGATCGACGACCGCACCGCCGTGCGCGCAGATCTCTCGCCGATGTTCGGAGAATTCGTCTGGACCAACG
CCTCCAACCATTTCGGCTGGGCGAGCCTTCTGGGGCAGCCGCCCGGTAGCCCGGACGTTTCGCCTTATGCAGCGGCCGCC
CGCGCAGAGAGCCTGGCCGGCCTGCCGCCCACCTTTATCTCTACCGGCGCGCTTGATCTCTTTACGGAGGAAAACCTTGA
ATATGCCCGCCGCCTGATGGCAGAGGGCGTTCCGGTGGAGCTTCATGTCTATCCCGGCGCCCCACATGCCTACATGCAGA
TGGCCGGCGCCCGCGTTACCCGCGCCTATATGCGTGACGCCTTGTCAGCGCTGGCGCGGGGACTGGGTGTGGACTGGAAG
GATTAG

Upstream 100 bases:

>100_bases
TCAACGGGATTCTCACCGGCGCGGGCGCTGGGGCAGGGCTCGACCCCGGCGGCGGAATGGGGAATGTTTGCCGCAAAGAA
AATGCTGCGGAGGAACCCCG

Downstream 100 bases:

>100_bases
ACCGAAGCCCGCAGCATCCAGGCGGTTTTCTCGGAAATCTGGGCGCGCTGGGTGAAGAGGTCGACGCTGACCGGGTCCGC
GTTCTTCTCGGCAATGGTGA

Product: alpha/beta fold family hydrolase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTSRPLVDPELIAFIEAFPTVNIGAENLAAVRQSRDAALAQLPPFPASVRVEEVFIPGPKGAPDVRLKLYRKKDGAEGPR
PAILHLHGGGYIMGNPEFMGPMCALWADAYDAVVVAPAYRIAPETAFPGNVEDAYAALAWANARAADLGIDTARIAVAGE
SAGGGLAASLALLVRDRGEYSFCHQQLIFPMIDDRTAVRADLSPMFGEFVWTNASNHFGWASLLGQPPGSPDVSPYAAAA
RAESLAGLPPTFISTGALDLFTEENLEYARRLMAEGVPVELHVYPGAPHAYMQMAGARVTRAYMRDALSALARGLGVDWK
D

Sequences:

>Translated_321_residues
MTSRPLVDPELIAFIEAFPTVNIGAENLAAVRQSRDAALAQLPPFPASVRVEEVFIPGPKGAPDVRLKLYRKKDGAEGPR
PAILHLHGGGYIMGNPEFMGPMCALWADAYDAVVVAPAYRIAPETAFPGNVEDAYAALAWANARAADLGIDTARIAVAGE
SAGGGLAASLALLVRDRGEYSFCHQQLIFPMIDDRTAVRADLSPMFGEFVWTNASNHFGWASLLGQPPGSPDVSPYAAAA
RAESLAGLPPTFISTGALDLFTEENLEYARRLMAEGVPVELHVYPGAPHAYMQMAGARVTRAYMRDALSALARGLGVDWK
D
>Mature_320_residues
TSRPLVDPELIAFIEAFPTVNIGAENLAAVRQSRDAALAQLPPFPASVRVEEVFIPGPKGAPDVRLKLYRKKDGAEGPRP
AILHLHGGGYIMGNPEFMGPMCALWADAYDAVVVAPAYRIAPETAFPGNVEDAYAALAWANARAADLGIDTARIAVAGES
AGGGLAASLALLVRDRGEYSFCHQQLIFPMIDDRTAVRADLSPMFGEFVWTNASNHFGWASLLGQPPGSPDVSPYAAAAR
AESLAGLPPTFISTGALDLFTEENLEYARRLMAEGVPVELHVYPGAPHAYMQMAGARVTRAYMRDALSALARGLGVDWKD

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=313, Percent_Identity=25.8785942492013, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI206597554, Length=321, Percent_Identity=26.1682242990654, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI21328446, Length=106, Percent_Identity=35.8490566037736, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI157041239, Length=111, Percent_Identity=34.2342342342342, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786682, Length=231, Percent_Identity=26.8398268398268, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=128, Percent_Identity=30.46875, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71996133, Length=192, Percent_Identity=28.125, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24656084, Length=134, Percent_Identity=31.3432835820896, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24656076, Length=134, Percent_Identity=31.3432835820896, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI20130169, Length=134, Percent_Identity=31.3432835820896, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34261; Mature: 34130

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRPLVDPELIAFIEAFPTVNIGAENLAAVRQSRDAALAQLPPFPASVRVEEVFIPGPK
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEEECCCC
GAPDVRLKLYRKKDGAEGPRPAILHLHGGGYIMGNPEFMGPMCALWADAYDAVVVAPAYR
CCCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHCHHHHHHHHHCCEEEECCCHH
IAPETAFPGNVEDAYAALAWANARAADLGIDTARIAVAGESAGGGLAASLALLVRDRGEY
CCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCH
SFCHQQLIFPMIDDRTAVRADLSPMFGEFVWTNASNHFGWASLLGQPPGSPDVSPYAAAA
HHHHHHHHHHHCCCCHHHHHCCCHHHHHEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHH
RAESLAGLPPTFISTGALDLFTEENLEYARRLMAEGVPVELHVYPGAPHAYMQMAGARVT
HHHHHCCCCCHHHCCCCEEEHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
RAYMRDALSALARGLGVDWKD
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSRPLVDPELIAFIEAFPTVNIGAENLAAVRQSRDAALAQLPPFPASVRVEEVFIPGPK
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEEECCCC
GAPDVRLKLYRKKDGAEGPRPAILHLHGGGYIMGNPEFMGPMCALWADAYDAVVVAPAYR
CCCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHCHHHHHHHHHCCEEEECCCHH
IAPETAFPGNVEDAYAALAWANARAADLGIDTARIAVAGESAGGGLAASLALLVRDRGEY
CCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCH
SFCHQQLIFPMIDDRTAVRADLSPMFGEFVWTNASNHFGWASLLGQPPGSPDVSPYAAAA
HHHHHHHHHHHCCCCHHHHHCCCHHHHHEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHH
RAESLAGLPPTFISTGALDLFTEENLEYARRLMAEGVPVELHVYPGAPHAYMQMAGARVT
HHHHHCCCCCHHHCCCCEEEHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
RAYMRDALSALARGLGVDWKD
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA