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The map label for this gene is cysG [H]

Identifier: 114800230

GI number: 114800230

Start: 317859

End: 319310

Strand: Direct

Name: cysG [H]

Synonym: HNE_0322

Alternate gene names: 114800230

Gene position: 317859-319310 (Clockwise)

Preceding gene: 114798902

Following gene: 114797102

Centisome position: 8.58

GC content: 68.39

Gene sequence:

>1452_bases
ATGCGCCAGTTCCCGGCCTTCTTTAACCTCGAAGGTGCCCGGATCGTCGTCTTTGGCGGCGGTGAAGAGGCCGCGCGAAA
GCTGCGCCTGTTTAACGATTCCGGCGCAGATGCCGTGCTTGTGGGCGGCTTTGAGGACGTGGCGCTCGCCGCAGAATTCG
CCCGCCGCGCAGAGACCGTGGCACGGGCTGACATGGCCCGCGCGCTGGACGGCGCCCGCCTCGTCATTATCGCCGAAGAA
GATGCAGGCCACCGCGCCCGCGCCCTCGCCGCCGTCCGCGCCCGCAACATTCCCGTCAATGTCGTCGATCAGCCCGAAGA
CTGCGATTTCACCGTCCCCTCCATTCTTGATCGCGGCGAGATTGTCGCCGCCATCGGCTCGGGCGGGGCTGCGCCTGTGC
TCGCCAAATCCATCCGCGCCAAGCTCGAAGCCCTGCTGCCCCAGCGCGTCGGCGATCTCGCCGCGCTCGCCCGCTCGCTG
CGCGGCTTTGTGGCCGAAGCAATTCCAGAGAAAACCGCCCGCCGCCGCTATTGGGAGCGCGCCTTTGCCGGCCCCGCCGC
CGAGGCCGCCTATGCCGGAGACCTTGAGCGCGCCGAAGCCCTCCTGCGCCAGCAGGCCCGCCTCACCGGCCGCGCGCGCG
GCGTGGTGCACATTGTCGGCGCTGGTCCCGGCGATCCGGAGCTTCTGACCCTCAAGGCCCTCCGCCTCATTCAGGAAGCG
GACGTCGTCTATTACGACCGCCTCGTCAGCCCGGAGATCATCTCCCTCATCCGCCGCGATGCTACCCGCGTGCCGGTCGG
CAAATCAAAGGGCGATCATTCCGTCCCGCAGACCGAAATCCATGCCCGCCTTGTCGCCTCGGCGCAGGAAGGCCTGCGCG
TCGTGCGCCTCAAGGGCGGCGACCCGTTCATCTTCGGCCGGGGCGGGGAAGAGCTGGAAGCCGTCCGCGCTGAAGGCATC
GAAGTTCACGTCGTGCCCGGCATTTCCTCGGCGCTCGGCTGCGCGGCCTCGGCCGGCGTCCCGCTCACCCACCGCGACCA
TGCCCAGACGCTCACCTTCGTCACCGGCCATGCCAAGGAAGGTGGCGTACCAGATCTTGACTGGCCCGCCCTCGCGCGCC
CGGCCCAGACGGTCGTTGTCTTCATGGGCGTTGATACCGCGCCTGTCATCGCCGAGAAACTTGTCGCTGCGGGCCGCGCC
CCGGAAACGCCCGTCGCCGTAATCGAGAATGGCACGCGCGCCGATGAACTGCGTGTCTTCGGCACGCTCGCCGAACTGCC
CTTCCTCGTGGAAGAAGAAGGCATCCGCGGCCCCGCGCTCCTCATCATCGGTGAGGTTGCGGGCCTCCCCGCAGAGCAGG
GCCGCATCGCCTCCATCGTCACTGAAGCGTCGGGTTTCGCCTGGTTTGGCGCCGATGCCCGCCGGCCAGATTTCAAGGAG
TCCGCCGCATGA

Upstream 100 bases:

>100_bases
TCTCTAAATGATCGCTGTAGGGGTTGCCCGTTGCGCCTGAGGCGTCAAGGAGGTTTTGTATATGCGTCCCTGTAGTGTCT
CTAATAAGAAAGTTGCGCCC

Downstream 100 bases:

>100_bases
CCTCCGTCCGCCCAAAGCTCAAGCCTGAAGTCCCCAAATCCGTCACCGCCTGGGAAACCGCCACCGGCCGCACCGTCTGG
CTGAAGGCCGATGGATCGTG

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MRQFPAFFNLEGARIVVFGGGEEAARKLRLFNDSGADAVLVGGFEDVALAAEFARRAETVARADMARALDGARLVIIAEE
DAGHRARALAAVRARNIPVNVVDQPEDCDFTVPSILDRGEIVAAIGSGGAAPVLAKSIRAKLEALLPQRVGDLAALARSL
RGFVAEAIPEKTARRRYWERAFAGPAAEAAYAGDLERAEALLRQQARLTGRARGVVHIVGAGPGDPELLTLKALRLIQEA
DVVYYDRLVSPEIISLIRRDATRVPVGKSKGDHSVPQTEIHARLVASAQEGLRVVRLKGGDPFIFGRGGEELEAVRAEGI
EVHVVPGISSALGCAASAGVPLTHRDHAQTLTFVTGHAKEGGVPDLDWPALARPAQTVVVFMGVDTAPVIAEKLVAAGRA
PETPVAVIENGTRADELRVFGTLAELPFLVEEEGIRGPALLIIGEVAGLPAEQGRIASIVTEASGFAWFGADARRPDFKE
SAA

Sequences:

>Translated_483_residues
MRQFPAFFNLEGARIVVFGGGEEAARKLRLFNDSGADAVLVGGFEDVALAAEFARRAETVARADMARALDGARLVIIAEE
DAGHRARALAAVRARNIPVNVVDQPEDCDFTVPSILDRGEIVAAIGSGGAAPVLAKSIRAKLEALLPQRVGDLAALARSL
RGFVAEAIPEKTARRRYWERAFAGPAAEAAYAGDLERAEALLRQQARLTGRARGVVHIVGAGPGDPELLTLKALRLIQEA
DVVYYDRLVSPEIISLIRRDATRVPVGKSKGDHSVPQTEIHARLVASAQEGLRVVRLKGGDPFIFGRGGEELEAVRAEGI
EVHVVPGISSALGCAASAGVPLTHRDHAQTLTFVTGHAKEGGVPDLDWPALARPAQTVVVFMGVDTAPVIAEKLVAAGRA
PETPVAVIENGTRADELRVFGTLAELPFLVEEEGIRGPALLIIGEVAGLPAEQGRIASIVTEASGFAWFGADARRPDFKE
SAA
>Mature_483_residues
MRQFPAFFNLEGARIVVFGGGEEAARKLRLFNDSGADAVLVGGFEDVALAAEFARRAETVARADMARALDGARLVIIAEE
DAGHRARALAAVRARNIPVNVVDQPEDCDFTVPSILDRGEIVAAIGSGGAAPVLAKSIRAKLEALLPQRVGDLAALARSL
RGFVAEAIPEKTARRRYWERAFAGPAAEAAYAGDLERAEALLRQQARLTGRARGVVHIVGAGPGDPELLTLKALRLIQEA
DVVYYDRLVSPEIISLIRRDATRVPVGKSKGDHSVPQTEIHARLVASAQEGLRVVRLKGGDPFIFGRGGEELEAVRAEGI
EVHVVPGISSALGCAASAGVPLTHRDHAQTLTFVTGHAKEGGVPDLDWPALARPAQTVVVFMGVDTAPVIAEKLVAAGRA
PETPVAVIENGTRADELRVFGTLAELPFLVEEEGIRGPALLIIGEVAGLPAEQGRIASIVTEASGFAWFGADARRPDFKE
SAA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=459, Percent_Identity=44.880174291939, Blast_Score=351, Evalue=5e-98,
Organism=Saccharomyces cerevisiae, GI6322922, Length=297, Percent_Identity=31.6498316498317, Blast_Score=141, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 51082; Mature: 51082

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQFPAFFNLEGARIVVFGGGEEAARKLRLFNDSGADAVLVGGFEDVALAAEFARRAETV
CCCCCCEEECCCCEEEEECCCHHHHHHHEEECCCCCCEEEECCCHHHHHHHHHHHHHHHH
ARADMARALDGARLVIIAEEDAGHRARALAAVRARNIPVNVVDQPEDCDFTVPSILDRGE
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHCCCC
IVAAIGSGGAAPVLAKSIRAKLEALLPQRVGDLAALARSLRGFVAEAIPEKTARRRYWER
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAGPAAEAAYAGDLERAEALLRQQARLTGRARGVVHIVGAGPGDPELLTLKALRLIQEA
HHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHC
DVVYYDRLVSPEIISLIRRDATRVPVGKSKGDHSVPQTEIHARLVASAQEGLRVVRLKGG
CHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
DPFIFGRGGEELEAVRAEGIEVHVVPGISSALGCAASAGVPLTHRDHAQTLTFVTGHAKE
CEEEECCCCHHHHHHHHCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
GGVPDLDWPALARPAQTVVVFMGVDTAPVIAEKLVAAGRAPETPVAVIENGTRADELRVF
CCCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHH
GTLAELPFLVEEEGIRGPALLIIGEVAGLPAEQGRIASIVTEASGFAWFGADARRPDFKE
HHHHHCCEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCHH
SAA
CCC
>Mature Secondary Structure
MRQFPAFFNLEGARIVVFGGGEEAARKLRLFNDSGADAVLVGGFEDVALAAEFARRAETV
CCCCCCEEECCCCEEEEECCCHHHHHHHEEECCCCCCEEEECCCHHHHHHHHHHHHHHHH
ARADMARALDGARLVIIAEEDAGHRARALAAVRARNIPVNVVDQPEDCDFTVPSILDRGE
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHCCCC
IVAAIGSGGAAPVLAKSIRAKLEALLPQRVGDLAALARSLRGFVAEAIPEKTARRRYWER
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAGPAAEAAYAGDLERAEALLRQQARLTGRARGVVHIVGAGPGDPELLTLKALRLIQEA
HHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHC
DVVYYDRLVSPEIISLIRRDATRVPVGKSKGDHSVPQTEIHARLVASAQEGLRVVRLKGG
CHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
DPFIFGRGGEELEAVRAEGIEVHVVPGISSALGCAASAGVPLTHRDHAQTLTFVTGHAKE
CEEEECCCCHHHHHHHHCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
GGVPDLDWPALARPAQTVVVFMGVDTAPVIAEKLVAAGRAPETPVAVIENGTRADELRVF
CCCCCCCCHHHCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHH
GTLAELPFLVEEEGIRGPALLIIGEVAGLPAEQGRIASIVTEASGFAWFGADARRPDFKE
HHHHHCCEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCHH
SAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA