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The map label for this gene is pyrB

Identifier: 114800183

GI number: 114800183

Start: 2399600

End: 2400547

Strand: Direct

Name: pyrB

Synonym: HNE_2302

Alternate gene names: 114800183

Gene position: 2399600-2400547 (Clockwise)

Preceding gene: 114797332

Following gene: 114798896

Centisome position: 64.77

GC content: 63.71

Gene sequence:

>948_bases
ATGGCGCCGCCTAGGCCAGACTACACCTTCACGCACGAGCATCTGCTCGGAATCGAAGGCCTTCATCCCCTCGATATTGG
TCATATTCTCGATCTGGCAGACAGCTATGCCGAGAAGACCCGCGCCGGTGTCCGCCCCGATCCGGTGCTGACCGGCAAGA
CGGTGGTAAACCTCTTCTTCGAGACTTCCACACGCACGATGTCGAGCTTCGAGATCGCTGCCCGGCGCCTCGGCGCGCAC
GTAATCTCGATGCCCATCGCCAATTCCTCGGTGAAGAAGGGCGAAACCCTCATCGACACGGCGATGACCCTCAACGCCAT
GAAGCCGGACGCGCTCGTCATCCGCCACTCCGCCTCGGGCGGCGCAAAGCTCCTCTCTCGCAAGGTCGACTGCGCCGTGA
TCAATGCCGGCGACGGCACCCATGAACACCCCACCCAGGCGCTGCTGGACCTGCTCACCATCCGCCGCGTCAAAAACCGC
ATCGAGGGCCTCAAAGTCGTCATCTGCGGCGACATCGCCCATAGCCGCGTCGCGCGCTCAACCACCATGGCGCTGCATCT
GATGGGCGCGCGCGTACATCTCTGCGGCCCCAAGACGCTGATCCCTCCGGGTACGGAGACATGGGGCGCCGAAAGCGCAG
GCACGGATTTCGACAAGGTGCTGAAAGGCGCAGACGTCGTCATGATGCTCCGCCTGCAGCTGGAACGCATGAACGGCTCC
TTCCTGCCCAGCCGCCGCGAATATTTCCGCTATTTCGGCCTCACCGCTGAGCGCCTCGCCCTCGCCAAGCCAAACGCCAC
CGTGATGCACCCCGGCCCGATGAACCGCGGCGTCGAAATCGAAAGCACCATCGCGGACGGGCCGAGCTCGGTCATCACCC
AGCAGGTCGAAATGGGCGTCGCCGTGCGCGAAGCCGTCCTCCACCTTCTCGCCGGAGGAAAATCATGA

Upstream 100 bases:

>100_bases
CTGCCGCTAACCTGGGCGCTGATCCTGTGGCTGGGGGCAGCCTGAAGGCTTGCCCTGCCCCAGAATCCCGCTTACACCGC
CGCAAATCTGAGGTATTCCG

Downstream 100 bases:

>100_bases
GCCTGCAGATAATCAATGCACGCCTGCTTGATCCGGCTACGGAGCTTGACGCGCCCGGCGCGATCCTCATCGAAGACGGC
AAGATCAGCGACATTCGCAC

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MAPPRPDYTFTHEHLLGIEGLHPLDIGHILDLADSYAEKTRAGVRPDPVLTGKTVVNLFFETSTRTMSSFEIAARRLGAH
VISMPIANSSVKKGETLIDTAMTLNAMKPDALVIRHSASGGAKLLSRKVDCAVINAGDGTHEHPTQALLDLLTIRRVKNR
IEGLKVVICGDIAHSRVARSTTMALHLMGARVHLCGPKTLIPPGTETWGAESAGTDFDKVLKGADVVMMLRLQLERMNGS
FLPSRREYFRYFGLTAERLALAKPNATVMHPGPMNRGVEIESTIADGPSSVITQQVEMGVAVREAVLHLLAGGKS

Sequences:

>Translated_315_residues
MAPPRPDYTFTHEHLLGIEGLHPLDIGHILDLADSYAEKTRAGVRPDPVLTGKTVVNLFFETSTRTMSSFEIAARRLGAH
VISMPIANSSVKKGETLIDTAMTLNAMKPDALVIRHSASGGAKLLSRKVDCAVINAGDGTHEHPTQALLDLLTIRRVKNR
IEGLKVVICGDIAHSRVARSTTMALHLMGARVHLCGPKTLIPPGTETWGAESAGTDFDKVLKGADVVMMLRLQLERMNGS
FLPSRREYFRYFGLTAERLALAKPNATVMHPGPMNRGVEIESTIADGPSSVITQQVEMGVAVREAVLHLLAGGKS
>Mature_314_residues
APPRPDYTFTHEHLLGIEGLHPLDIGHILDLADSYAEKTRAGVRPDPVLTGKTVVNLFFETSTRTMSSFEIAARRLGAHV
ISMPIANSSVKKGETLIDTAMTLNAMKPDALVIRHSASGGAKLLSRKVDCAVINAGDGTHEHPTQALLDLLTIRRVKNRI
EGLKVVICGDIAHSRVARSTTMALHLMGARVHLCGPKTLIPPGTETWGAESAGTDFDKVLKGADVVMMLRLQLERMNGSF
LPSRREYFRYFGLTAERLALAKPNATVMHPGPMNRGVEIESTIADGPSSVITQQVEMGVAVREAVLHLLAGGKS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=33.7579617834395, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=35.2750809061489, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1790703, Length=195, Percent_Identity=30.2564102564103, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786469, Length=329, Percent_Identity=24.6200607902736, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=34.5794392523364, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=322, Percent_Identity=34.7826086956522, Blast_Score=142, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=33.968253968254, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_HYPNA (Q0BZU6)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760997.1
- ProteinModelPortal:   Q0BZU6
- SMR:   Q0BZU6
- STRING:   Q0BZU6
- GeneID:   4290027
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2302
- NMPDR:   fig|228405.5.peg.2218
- TIGR:   HNE_2302
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- PhylomeDB:   Q0BZU6
- BioCyc:   HNEP81032:HNE_2302-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34036; Mature: 33905

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPPRPDYTFTHEHLLGIEGLHPLDIGHILDLADSYAEKTRAGVRPDPVLTGKTVVNLFF
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEE
ETSTRTMSSFEIAARRLGAHVISMPIANSSVKKGETLIDTAMTLNAMKPDALVIRHSASG
CCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCC
GAKLLSRKVDCAVINAGDGTHEHPTQALLDLLTIRRVKNRIEGLKVVICGDIAHSRVARS
CHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHH
TTMALHLMGARVHLCGPKTLIPPGTETWGAESAGTDFDKVLKGADVVMMLRLQLERMNGS
HHHHEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
FLPSRREYFRYFGLTAERLALAKPNATVMHPGPMNRGVEIESTIADGPSSVITQQVEMGV
CCCCHHHHHHHHCCCHHHHEEECCCCEEECCCCCCCCEEEEHHHCCCCHHHHHHHHHHHH
AVREAVLHLLAGGKS
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
APPRPDYTFTHEHLLGIEGLHPLDIGHILDLADSYAEKTRAGVRPDPVLTGKTVVNLFF
CCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEE
ETSTRTMSSFEIAARRLGAHVISMPIANSSVKKGETLIDTAMTLNAMKPDALVIRHSASG
CCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCC
GAKLLSRKVDCAVINAGDGTHEHPTQALLDLLTIRRVKNRIEGLKVVICGDIAHSRVARS
CHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHH
TTMALHLMGARVHLCGPKTLIPPGTETWGAESAGTDFDKVLKGADVVMMLRLQLERMNGS
HHHHEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
FLPSRREYFRYFGLTAERLALAKPNATVMHPGPMNRGVEIESTIADGPSSVITQQVEMGV
CCCCHHHHHHHHCCCHHHHEEECCCCEEECCCCCCCCEEEEHHHCCCCHHHHHHHHHHHH
AVREAVLHLLAGGKS
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA