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The map label for this gene is ruvC

Identifier: 114799962

GI number: 114799962

Start: 128909

End: 129412

Strand: Direct

Name: ruvC

Synonym: HNE_0145

Alternate gene names: 114799962

Gene position: 128909-129412 (Clockwise)

Preceding gene: 114797636

Following gene: 114798884

Centisome position: 3.48

GC content: 67.26

Gene sequence:

>504_bases
ATGAGCACCCCGATTCGAATTCTCGGCATTGATCCGGGCCTCCGCCATACCGGCTGGGGCGTGATAGAGCAGTCTGGCGC
CCGCCTGGTGCATATCGCCCATGGCGTGATCGACCCGCCGACCGACCTCTCGATGGCTGAGCGGCTGGGGCATATTTTCG
AGGCGGTGGGCGAACTGGCTCGCCATCATGCGCCCCATGCGGCCGGGGTTGAGGAAACGTTGGTGAACGCAAACCCCCGC
TCGGCGCTGAAACTGGGCCAGGCGCGCGGGGCGGCGATGGCGGCGCTGGCGATGGCGGGTGTTTCGGTCGCTGAGTTTGC
GCCCCGGCAGATCAAGCTCTCCGTCGTCGGCACCGGCACGGCCGACAAGGATCAGGTGAAATTCATGGTCCAGCGCCTGC
TTCCCAGGGCAGGGGAGATGAAGCTCGACGCCGCCGACGCGCTTGCCTGCGCCATCTGCGCGGCGCACCATCTGCCGCTG
CAGATGAAGCGGGGCGCGGCATGA

Upstream 100 bases:

>100_bases
TCTGCCCCTTCCGGCAGGCGCGGCTTTTTTGTCAGGACCGCGAACAAAAAGTGAAACTGGCAAGATTCCATTAACCGCCC
AGCCTGTTAGGGTGTCGCGC

Downstream 100 bases:

>100_bases
TTATAGGTCGTCTCAAGGGCGAGGTTGCGGCCATCGGGCTCGACCATGTGCTGATTGACGTAAACGGGGTGGGCTATGTC
GCCCTCGCGGGAACGCGCCT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSTPIRILGIDPGLRHTGWGVIEQSGARLVHIAHGVIDPPTDLSMAERLGHIFEAVGELARHHAPHAAGVEETLVNANPR
SALKLGQARGAAMAALAMAGVSVAEFAPRQIKLSVVGTGTADKDQVKFMVQRLLPRAGEMKLDAADALACAICAAHHLPL
QMKRGAA

Sequences:

>Translated_167_residues
MSTPIRILGIDPGLRHTGWGVIEQSGARLVHIAHGVIDPPTDLSMAERLGHIFEAVGELARHHAPHAAGVEETLVNANPR
SALKLGQARGAAMAALAMAGVSVAEFAPRQIKLSVVGTGTADKDQVKFMVQRLLPRAGEMKLDAADALACAICAAHHLPL
QMKRGAA
>Mature_166_residues
STPIRILGIDPGLRHTGWGVIEQSGARLVHIAHGVIDPPTDLSMAERLGHIFEAVGELARHHAPHAAGVEETLVNANPRS
ALKLGQARGAAMAALAMAGVSVAEFAPRQIKLSVVGTGTADKDQVKFMVQRLLPRAGEMKLDAADALACAICAAHHLPLQ
MKRGAA

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=128, Percent_Identity=44.53125, Blast_Score=103, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_HYPNA (Q0C5W4)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758879.1
- ProteinModelPortal:   Q0C5W4
- SMR:   Q0C5W4
- STRING:   Q0C5W4
- GeneID:   4290117
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0145
- NMPDR:   fig|228405.5.peg.143
- TIGR:   HNE_0145
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q0C5W4
- ProtClustDB:   PRK00039
- BioCyc:   HNEP81032:HNE_0145-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17508; Mature: 17376

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPIRILGIDPGLRHTGWGVIEQSGARLVHIAHGVIDPPTDLSMAERLGHIFEAVGELA
CCCCEEEEECCCCCCCCCCCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RHHAPHAAGVEETLVNANPRSALKLGQARGAAMAALAMAGVSVAEFAPRQIKLSVVGTGT
HHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEECCC
ADKDQVKFMVQRLLPRAGEMKLDAADALACAICAAHHLPLQMKRGAA
CCHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCHHCCCCC
>Mature Secondary Structure 
STPIRILGIDPGLRHTGWGVIEQSGARLVHIAHGVIDPPTDLSMAERLGHIFEAVGELA
CCCEEEEECCCCCCCCCCCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RHHAPHAAGVEETLVNANPRSALKLGQARGAAMAALAMAGVSVAEFAPRQIKLSVVGTGT
HHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEECCC
ADKDQVKFMVQRLLPRAGEMKLDAADALACAICAAHHLPLQMKRGAA
CCHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA