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The map label for this gene is rfbB [C]

Identifier: 114799940

GI number: 114799940

Start: 802980

End: 804077

Strand: Reverse

Name: rfbB [C]

Synonym: HNE_0790

Alternate gene names: 114799940

Gene position: 804077-802980 (Counterclockwise)

Preceding gene: 114798886

Following gene: 114797641

Centisome position: 21.7

GC content: 60.56

Gene sequence:

>1098_bases
ATGCAGACCAACAGTCCTCCTGTGAAAGTCCTAGTAACAGGCGGCGCCGGCTTCATCGGGTCCGCGCTCGTCCGTCATCT
TATTGATACCACGCACGCCCGTGTAACCGTGGTGGACAAGCTCACTTACGCGGCCAATCTGGACTCCCTCGCGCCGGTCT
CCGCCTCGCCGCGCTACACATTCATCCGCGAAGACATCTGCAATGCCCCCGCGATGCGCGACATATTCCTAGCCGAACGT
CCCGATTATGTCCTCCACCTTGCCGCTGAAACGCATGTAGACCGCTCCATTTCCGGCTCGCAGCAATTCATTCAGACCAA
TATCAACGGTACCTACAATCTTCTTGAGGCCGCCCGCGCCTTGCAGGCAGAGCGCTCCGGCGACCTGCGCAGCCGGTTTC
GCTTCCTTCATGTGTCCACCGATGAAGTGTACGGCTCGCTCGGGCCCGACGGTCTCTTCAGCGAGACCACAGCCTATGAT
CCGTCCTCGCCCTATTCGGCCTCAAAGGCCGCGTCAGACCATCTGGCCAAGGCCTGGCACCGCACCTATGGCCTGCCGGT
CATTGTGTCGAACTGCTCAAACAATTACGGCCCGTATCACTTTCCCGAAAAGCTCATTCCGCTGATCATTCTGAACGCGC
TCGAGGGACAGAGGCTGCCGGTCTATGGCGATGGATCGAACATACGCGACTGGCTGCATGTGGAAGATCATGCCCGCGCG
CTGTGGAGGATCGCCACGACGGGACGGCCCGGCGAAACCTATAATGTCGGCGGGCTGAACGAGCGCACCAATCTCCAGGT
CGTTCACACGATCTGTGACCTCCTCGACGAACTGCGGCCCGCAAACCGGCCGCGCCGGGAGCTGATCGAATTTGTTTCCG
ACCGGCCCGGCCACGATCAGCGCTATGCGATTGACGCAGCCAAACTTCAGACCGAGCTCGGCTGGAAAGCGGAGGAAACC
TTCGACACCGGCATCCGGAAAACGGTCGAATGGTATATCGATCACCCCGAATGGTGGGGGCCGTTGCGCAAATCGGTATA
TTCCGGAGACCGGCTCGGCCTCCTCGCCGCCTCCGGCGAGCCGGCCGCAAAAGACTGA

Upstream 100 bases:

>100_bases
CTGGCCCCTCCAGGGGAACGCCCCCATCCAGTCCGAAAAAGTCGCCGCGCAGCCCAACCTGTCGGACCTTCCCGACTATT
TCCAGTATTCCAGAAGCTAG

Downstream 100 bases:

>100_bases
CACGCCTCCCGATACGCTGCAGAATGCACACATAGGCGATTGCAATGCCCGCACGCCGCGATAGCTTGCGCGGCCAGTGC
TGCCGGCGGGTAAAACAGGA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MQTNSPPVKVLVTGGAGFIGSALVRHLIDTTHARVTVVDKLTYAANLDSLAPVSASPRYTFIREDICNAPAMRDIFLAER
PDYVLHLAAETHVDRSISGSQQFIQTNINGTYNLLEAARALQAERSGDLRSRFRFLHVSTDEVYGSLGPDGLFSETTAYD
PSSPYSASKAASDHLAKAWHRTYGLPVIVSNCSNNYGPYHFPEKLIPLIILNALEGQRLPVYGDGSNIRDWLHVEDHARA
LWRIATTGRPGETYNVGGLNERTNLQVVHTICDLLDELRPANRPRRELIEFVSDRPGHDQRYAIDAAKLQTELGWKAEET
FDTGIRKTVEWYIDHPEWWGPLRKSVYSGDRLGLLAASGEPAAKD

Sequences:

>Translated_365_residues
MQTNSPPVKVLVTGGAGFIGSALVRHLIDTTHARVTVVDKLTYAANLDSLAPVSASPRYTFIREDICNAPAMRDIFLAER
PDYVLHLAAETHVDRSISGSQQFIQTNINGTYNLLEAARALQAERSGDLRSRFRFLHVSTDEVYGSLGPDGLFSETTAYD
PSSPYSASKAASDHLAKAWHRTYGLPVIVSNCSNNYGPYHFPEKLIPLIILNALEGQRLPVYGDGSNIRDWLHVEDHARA
LWRIATTGRPGETYNVGGLNERTNLQVVHTICDLLDELRPANRPRRELIEFVSDRPGHDQRYAIDAAKLQTELGWKAEET
FDTGIRKTVEWYIDHPEWWGPLRKSVYSGDRLGLLAASGEPAAKD
>Mature_365_residues
MQTNSPPVKVLVTGGAGFIGSALVRHLIDTTHARVTVVDKLTYAANLDSLAPVSASPRYTFIREDICNAPAMRDIFLAER
PDYVLHLAAETHVDRSISGSQQFIQTNINGTYNLLEAARALQAERSGDLRSRFRFLHVSTDEVYGSLGPDGLFSETTAYD
PSSPYSASKAASDHLAKAWHRTYGLPVIVSNCSNNYGPYHFPEKLIPLIILNALEGQRLPVYGDGSNIRDWLHVEDHARA
LWRIATTGRPGETYNVGGLNERTNLQVVHTICDLLDELRPANRPRRELIEFVSDRPGHDQRYAIDAAKLQTELGWKAEET
FDTGIRKTVEWYIDHPEWWGPLRKSVYSGDRLGLLAASGEPAAKD

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=339, Percent_Identity=34.8082595870207, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI42516563, Length=348, Percent_Identity=25.8620689655172, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=63.3522727272727, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1788353, Length=349, Percent_Identity=63.323782234957, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1786974, Length=363, Percent_Identity=28.6501377410468, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788366, Length=350, Percent_Identity=24, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=34.9397590361446, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=32.1212121212121, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71982038, Length=366, Percent_Identity=26.2295081967213, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=364, Percent_Identity=26.3736263736264, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=23.3333333333333, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=252, Percent_Identity=24.6031746031746, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=344, Percent_Identity=22.6744186046512, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=344, Percent_Identity=22.6744186046512, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=344, Percent_Identity=22.6744186046512, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=348, Percent_Identity=22.7011494252874, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=344, Percent_Identity=22.6744186046512, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=368, Percent_Identity=25.8152173913043, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19923002, Length=366, Percent_Identity=27.5956284153005, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=26.0606060606061, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40620; Mature: 40620

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNSPPVKVLVTGGAGFIGSALVRHLIDTTHARVTVVDKLTYAANLDSLAPVSASPRYT
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCCCCCCCEE
FIREDICNAPAMRDIFLAERPDYVLHLAAETHVDRSISGSQQFIQTNINGTYNLLEAARA
EHHHHHCCCCCHHHHHHCCCCCEEEEEEHHHCCCCCCCCCHHHEEECCCCHHHHHHHHHH
LQAERSGDLRSRFRFLHVSTDEVYGSLGPDGLFSETTAYDPSSPYSASKAASDHLAKAWH
HHHHCCCCHHHHEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RTYGLPVIVSNCSNNYGPYHFPEKLIPLIILNALEGQRLPVYGDGSNIRDWLHVEDHARA
HHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHEEHHHHHHH
LWRIATTGRPGETYNVGGLNERTNLQVVHTICDLLDELRPANRPRRELIEFVSDRPGHDQ
HHHEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
RYAIDAAKLQTELGWKAEETFDTGIRKTVEWYIDHPEWWGPLRKSVYSGDRLGLLAASGE
EEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCEEEEEECCC
PAAKD
CCCCC
>Mature Secondary Structure
MQTNSPPVKVLVTGGAGFIGSALVRHLIDTTHARVTVVDKLTYAANLDSLAPVSASPRYT
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCCCCCCCEE
FIREDICNAPAMRDIFLAERPDYVLHLAAETHVDRSISGSQQFIQTNINGTYNLLEAARA
EHHHHHCCCCCHHHHHHCCCCCEEEEEEHHHCCCCCCCCCHHHEEECCCCHHHHHHHHHH
LQAERSGDLRSRFRFLHVSTDEVYGSLGPDGLFSETTAYDPSSPYSASKAASDHLAKAWH
HHHHCCCCHHHHEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RTYGLPVIVSNCSNNYGPYHFPEKLIPLIILNALEGQRLPVYGDGSNIRDWLHVEDHARA
HHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHEEHHHHHHH
LWRIATTGRPGETYNVGGLNERTNLQVVHTICDLLDELRPANRPRRELIEFVSDRPGHDQ
HHHEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
RYAIDAAKLQTELGWKAEETFDTGIRKTVEWYIDHPEWWGPLRKSVYSGDRLGLLAASGE
EEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCEEEEEECCC
PAAKD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]