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The map label for this gene is rfbA [H]

Identifier: 114797641

GI number: 114797641

Start: 801993

End: 802868

Strand: Reverse

Name: rfbA [H]

Synonym: HNE_0789

Alternate gene names: 114797641

Gene position: 802868-801993 (Counterclockwise)

Preceding gene: 114799940

Following gene: 114798407

Centisome position: 21.67

GC content: 62.79

Gene sequence:

>876_bases
ATGAAGGGCATCATTCTGGCGGGCGGAAAAGGTACGCGCCTCTATCCGATCACGCGCGGCACCTCCAAACAATTGCTGCC
GATCTATGACAAGCCGATGGTCTACCATCCGATCACGACGCTGATGCTTGCGGGCATTCGCGAAATCCTCGTGATCGCCA
TGCCCGATTCCCTGACAGCCTATCAGCGGCTGCTGGGCACCGGCGAGGATTGGGGCATGAGCTTCACCTACGCCCCCCAG
CCAGAGCCACGCGGCCTTGCAGAGGCCTACATCATCGGCGCCGGCTTCGTGAACGGCCAGAGCAGCGCGCTGGCCCTTGG
GGACAACATGTTCTACGGCGCCGGCCTCGGCGGGGAGCTGGAAAAGTTCGGAAAGCTTGAAACCGGCGCCACGGTGCTCG
CCTGCCAGGTCAACAACCCCTCCGACTTCGGCATCGTTGAAATGGGCGCCGACGGCAAAGCCATCTCCATCGAGGAAAAA
CCCGCCCGCCCCAAAAGCAACTGGGCGGTCACCGGCCTCTATTTCTACGACAAGGACGTGGTCGACATTGCAAAGGACGT
CCAGTCCTCCGCCCGCGGCGAGCTGGAAATCACCTCGGTCAACGAAGTTTACCTCCAGCGCGGCAACCTCAACGTCCATC
GCCTCGCCCGCGGCACCGCCTGGCTCGACGCCGGCACTTTTGACGGCCTCATCCAGGCGTCCCAGTTCGTGCAGACGGTC
GAAAAGCGCCAGGGCATGAAGATCGCCTGCCCCGAGGAAGTCGCCTGGCGCAAGCGCTTCATCGACGACGCGCAGCTCGA
AAAGCTCGCCGCCGGCTACCGCAATGAGTACGGCCAATACCTGCAAAGCCTGCTCACCGCGCCTTTCCGGGAATAG

Upstream 100 bases:

>100_bases
ATACGCTGCAGAATGCACACATAGGCGATTGCAATGCCCGCACGCCGCGATAGCTTGCGCGGCCAGTGCTGCCGGCGGGT
AAAACAGGATTGTAGATTCA

Downstream 100 bases:

>100_bases
GGTCGCGCCCCAACAGACCATCCGGCCAATTCAACGCAGCTTGCAGCGGCAAAACCTTGCGCGTGCCCACCGGCCATGTT
AACCGCAGCCCAACATATAA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2 [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKGIILAGGKGTRLYPITRGTSKQLLPIYDKPMVYHPITTLMLAGIREILVIAMPDSLTAYQRLLGTGEDWGMSFTYAPQ
PEPRGLAEAYIIGAGFVNGQSSALALGDNMFYGAGLGGELEKFGKLETGATVLACQVNNPSDFGIVEMGADGKAISIEEK
PARPKSNWAVTGLYFYDKDVVDIAKDVQSSARGELEITSVNEVYLQRGNLNVHRLARGTAWLDAGTFDGLIQASQFVQTV
EKRQGMKIACPEEVAWRKRFIDDAQLEKLAAGYRNEYGQYLQSLLTAPFRE

Sequences:

>Translated_291_residues
MKGIILAGGKGTRLYPITRGTSKQLLPIYDKPMVYHPITTLMLAGIREILVIAMPDSLTAYQRLLGTGEDWGMSFTYAPQ
PEPRGLAEAYIIGAGFVNGQSSALALGDNMFYGAGLGGELEKFGKLETGATVLACQVNNPSDFGIVEMGADGKAISIEEK
PARPKSNWAVTGLYFYDKDVVDIAKDVQSSARGELEITSVNEVYLQRGNLNVHRLARGTAWLDAGTFDGLIQASQFVQTV
EKRQGMKIACPEEVAWRKRFIDDAQLEKLAAGYRNEYGQYLQSLLTAPFRE
>Mature_291_residues
MKGIILAGGKGTRLYPITRGTSKQLLPIYDKPMVYHPITTLMLAGIREILVIAMPDSLTAYQRLLGTGEDWGMSFTYAPQ
PEPRGLAEAYIIGAGFVNGQSSALALGDNMFYGAGLGGELEKFGKLETGATVLACQVNNPSDFGIVEMGADGKAISIEEK
PARPKSNWAVTGLYFYDKDVVDIAKDVQSSARGELEITSVNEVYLQRGNLNVHRLARGTAWLDAGTFDGLIQASQFVQTV
EKRQGMKIACPEEVAWRKRFIDDAQLEKLAAGYRNEYGQYLQSLLTAPFRE

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790224, Length=292, Percent_Identity=59.5890410958904, Blast_Score=353, Evalue=6e-99,
Organism=Escherichia coli, GI1788351, Length=284, Percent_Identity=57.7464788732394, Blast_Score=347, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI133931050, Length=228, Percent_Identity=24.1228070175439, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320148, Length=259, Percent_Identity=25.8687258687259, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21355443, Length=206, Percent_Identity=24.2718446601942, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24644084, Length=206, Percent_Identity=24.2718446601942, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 31841; Mature: 31841

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGKGTRLYPITRGTSKQLLPIYDKPMVYHPITTLMLAGIREILVIAMPDSLTA
CCCEEEECCCCCEEEEEECCCCCCCCEECCCCEEEHHHHHHHHHHHHHEEEEECCCHHHH
YQRLLGTGEDWGMSFTYAPQPEPRGLAEAYIIGAGFVNGQSSALALGDNMFYGAGLGGEL
HHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEEECCCCCEEEECCCEEEECCCCCHH
EKFGKLETGATVLACQVNNPSDFGIVEMGADGKAISIEEKPARPKSNWAVTGLYFYDKDV
HHHCCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCHH
VDIAKDVQSSARGELEITSVNEVYLQRGNLNVHRLARGTAWLDAGTFDGLIQASQFVQTV
HHHHHHHHHHCCCCEEEEEHHEEEEECCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHH
EKRQGMKIACPEEVAWRKRFIDDAQLEKLAAGYRNEYGQYLQSLLTAPFRE
HHHCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKGIILAGGKGTRLYPITRGTSKQLLPIYDKPMVYHPITTLMLAGIREILVIAMPDSLTA
CCCEEEECCCCCEEEEEECCCCCCCCEECCCCEEEHHHHHHHHHHHHHEEEEECCCHHHH
YQRLLGTGEDWGMSFTYAPQPEPRGLAEAYIIGAGFVNGQSSALALGDNMFYGAGLGGEL
HHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEEECCCCCEEEECCCEEEECCCCCHH
EKFGKLETGATVLACQVNNPSDFGIVEMGADGKAISIEEKPARPKSNWAVTGLYFYDKDV
HHHCCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCHH
VDIAKDVQSSARGELEITSVNEVYLQRGNLNVHRLARGTAWLDAGTFDGLIQASQFVQTV
HHHHHHHHHHCCCCEEEEEHHEEEEECCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHH
EKRQGMKIACPEEVAWRKRFIDDAQLEKLAAGYRNEYGQYLQSLLTAPFRE
HHHCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]