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The map label for this gene is dksA [H]

Identifier: 114799932

GI number: 114799932

Start: 494368

End: 494835

Strand: Direct

Name: dksA [H]

Synonym: HNE_0503

Alternate gene names: 114799932

Gene position: 494368-494835 (Clockwise)

Preceding gene: 114800047

Following gene: 114798089

Centisome position: 13.34

GC content: 61.97

Gene sequence:

>468_bases
GTGAATCGAGGTCCGCTCTATATGGCACGCAAACGAGAGAAGGAGTCTGCCTTGAGTATCGACCTCTCAGACGATTATCG
CCCCTCGGAAGACGAAGAGTTCATGAATGAGCGGCAACTCGCCTATTTCAGGCGGCTGCTGGAAGCCTGGAAGGCCGACA
TCCTGGACGGCGCCAAGCAGACCATTCACAATCTTCAGGACGAGTCAGGCTCTCTCCCCGACATTGTCGACCGCGCCTCG
GCCGAAAGCGACAAAGCCCTAGAGCTGCGCACACGGGACCGCCAGCGCAAGCTGATCTCGAAAATCGACTCCGCCCTGCG
CCGTATTGATGACGGCTCTTACGGGTTTTGCGAAGCGACGGGCGAGCCGATCGGCCTGCGCCGCCTCGAAGCGCGCCCGA
CCGCAACGCTGAGCCTTGAGGCCCAGGAGCGCCACGAGCGCAAGGAGCGCACCCTGCGGGACGACTAG

Upstream 100 bases:

>100_bases
CATTGTGGGGCATGGAATTGCCTTAAGACAGGGTTTTGGCACGTTATTGTCGTAATTGTCTGATATTGCAGGATTTACTA
CCGCAGTCTCGGAGATTGCA

Downstream 100 bases:

>100_bases
TCCGGTCTTTCTGACACAAAGCAGACAGCAAAAAGGCGGCTCTCCAGATCGGAGTGCCGCCTTTTTTCCAGTCATCTTCC
CCACGCCTTTGGCGGATGTT

Product: RNA polymerase-binding protein DksA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MNRGPLYMARKREKESALSIDLSDDYRPSEDEEFMNERQLAYFRRLLEAWKADILDGAKQTIHNLQDESGSLPDIVDRAS
AESDKALELRTRDRQRKLISKIDSALRRIDDGSYGFCEATGEPIGLRRLEARPTATLSLEAQERHERKERTLRDD

Sequences:

>Translated_155_residues
MNRGPLYMARKREKESALSIDLSDDYRPSEDEEFMNERQLAYFRRLLEAWKADILDGAKQTIHNLQDESGSLPDIVDRAS
AESDKALELRTRDRQRKLISKIDSALRRIDDGSYGFCEATGEPIGLRRLEARPTATLSLEAQERHERKERTLRDD
>Mature_155_residues
MNRGPLYMARKREKESALSIDLSDDYRPSEDEEFMNERQLAYFRRLLEAWKADILDGAKQTIHNLQDESGSLPDIVDRAS
AESDKALELRTRDRQRKLISKIDSALRRIDDGSYGFCEATGEPIGLRRLEARPTATLSLEAQERHERKERTLRDD

Specific function: Dosage-Dependent Suppressor Of A Dnak Deletion Mutation. It Suppressed Not Only The Temperature-Sensitive Growth But Also The Filamentous Phenotype Of The Dnak Deletion Strain, While The Defect Of Lambda Growth Is Not Suppressed. [C]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=143, Percent_Identity=46.1538461538462, Blast_Score=136, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 17967; Mature: 17967

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01102 ZF_DKSA_1 ; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRGPLYMARKREKESALSIDLSDDYRPSEDEEFMNERQLAYFRRLLEAWKADILDGAKQ
CCCCCCHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIHNLQDESGSLPDIVDRASAESDKALELRTRDRQRKLISKIDSALRRIDDGSYGFCEAT
HHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
GEPIGLRRLEARPTATLSLEAQERHERKERTLRDD
CCCCCCEEECCCCCEEEEECHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNRGPLYMARKREKESALSIDLSDDYRPSEDEEFMNERQLAYFRRLLEAWKADILDGAKQ
CCCCCCHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIHNLQDESGSLPDIVDRASAESDKALELRTRDRQRKLISKIDSALRRIDDGSYGFCEAT
HHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
GEPIGLRRLEARPTATLSLEAQERHERKERTLRDD
CCCCCCEEECCCCCEEEEECHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9555902 [H]