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The map label for this gene is thiD [H]

Identifier: 114799777

GI number: 114799777

Start: 3401871

End: 3402689

Strand: Reverse

Name: thiD [H]

Synonym: HNE_3234

Alternate gene names: 114799777

Gene position: 3402689-3401871 (Counterclockwise)

Preceding gene: 114799016

Following gene: 114797308

Centisome position: 91.84

GC content: 67.77

Gene sequence:

>819_bases
ATGGCGGAGGCAGAAGGCCCGCGCGGCCGTGTCCTGATCATTGCCGGCTCCGATTCCGGGGGCGGGGCGGGCATTCAAGC
CGACATCAAGACCGTGACGATGCTGGGCGGCTTCGCGATGACGGCGGTGACCGCCATCACGGTGCAGGACACGACCGGCG
TGCATGGCGTGTGGCCTGTGCCGGTCGAGACCGTGACCGCGCAGATGCAGGTGACGCTGGCTGACATTGGCGCTGATGCG
ATCAAGACCGGCATGCTGGGCACCGCGCCGCTGGTCGAGGCGGTGGCGGAAATGCTGGAGGCGCGCGCGTTCGGGATTTC
GCGCGTCATTGATCCGGTGATGATTTCGACCTCCGGGCACAGGCTCGTGGACGAGCGGGCGATCGGCGCGATCCGCGCGG
AGCTGGTGCCGCGCGCGCGCCTGGTGACGCCGAATGCGCCGGAGGCTGAAGTGCTTACGGGCAAGGCGGTCGATACGCTC
GACGGCCAGCGCCGGGCGGCAGAGCGGCTGCTGGAGCTGGGCGCCCATGGCGCGCTGGTGAAGGGCGGGCATGTGGGCGG
CTCAATGATTCACGACGTGTTGCAGACGACGACCGGCGAGTGGATTTTCGAGGGGCCGCGCATCGATACGCAGGCAACCC
ATGGCACGGGCTGCACGCTGGCCTCGGCAATTGCCGCGCTGTTGGCGCAAGGGGTGAGCCTGCCCGAAGCAATCGAGCGG
GCGCGGGCGTATCTGTTTGGCGCGATCCAGAATGCACAGGGGTTTGGCAAGGGCCATCACCCGGTCAATCATGGCTGGAC
GCTGAAGGCGGATGATTAG

Upstream 100 bases:

>100_bases
CGGCAGTGATCCGTAGTCTTGTCAGCCATCCGTTGACGGATAAAGGTCTTGAGACCTTCCTTGCGGACGCGAAGAAGGCC
GGCATCGAGGTCTGATCCCT

Downstream 100 bases:

>100_bases
CCAGGCTTAACGGCCCTGCAGAAAACTTCATTTTACGCTGCCGGTGGGCCATGCCAGAGAAGGGCTCGCTTCAGCGGAGC
CGGTTACATGCCCACTTATC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MAEAEGPRGRVLIIAGSDSGGGAGIQADIKTVTMLGGFAMTAVTAITVQDTTGVHGVWPVPVETVTAQMQVTLADIGADA
IKTGMLGTAPLVEAVAEMLEARAFGISRVIDPVMISTSGHRLVDERAIGAIRAELVPRARLVTPNAPEAEVLTGKAVDTL
DGQRRAAERLLELGAHGALVKGGHVGGSMIHDVLQTTTGEWIFEGPRIDTQATHGTGCTLASAIAALLAQGVSLPEAIER
ARAYLFGAIQNAQGFGKGHHPVNHGWTLKADD

Sequences:

>Translated_272_residues
MAEAEGPRGRVLIIAGSDSGGGAGIQADIKTVTMLGGFAMTAVTAITVQDTTGVHGVWPVPVETVTAQMQVTLADIGADA
IKTGMLGTAPLVEAVAEMLEARAFGISRVIDPVMISTSGHRLVDERAIGAIRAELVPRARLVTPNAPEAEVLTGKAVDTL
DGQRRAAERLLELGAHGALVKGGHVGGSMIHDVLQTTTGEWIFEGPRIDTQATHGTGCTLASAIAALLAQGVSLPEAIER
ARAYLFGAIQNAQGFGKGHHPVNHGWTLKADD
>Mature_271_residues
AEAEGPRGRVLIIAGSDSGGGAGIQADIKTVTMLGGFAMTAVTAITVQDTTGVHGVWPVPVETVTAQMQVTLADIGADAI
KTGMLGTAPLVEAVAEMLEARAFGISRVIDPVMISTSGHRLVDERAIGAIRAELVPRARLVTPNAPEAEVLTGKAVDTLD
GQRRAAERLLELGAHGALVKGGHVGGSMIHDVLQTTTGEWIFEGPRIDTQATHGTGCTLASAIAALLAQGVSLPEAIERA
RAYLFGAIQNAQGFGKGHHPVNHGWTLKADD

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=261, Percent_Identity=38.6973180076628, Blast_Score=170, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324997, Length=280, Percent_Identity=37.1428571428571, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324517, Length=280, Percent_Identity=37.5, Blast_Score=129, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325378, Length=279, Percent_Identity=35.1254480286738, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28097; Mature: 27966

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAEGPRGRVLIIAGSDSGGGAGIQADIKTVTMLGGFAMTAVTAITVQDTTGVHGVWPV
CCCCCCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
PVETVTAQMQVTLADIGADAIKTGMLGTAPLVEAVAEMLEARAFGISRVIDPVMISTSGH
CHHHHHHEEEEEHHHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCC
RLVDERAIGAIRAELVPRARLVTPNAPEAEVLTGKAVDTLDGQRRAAERLLELGAHGALV
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCHHHCCHHHHHHHHHHHHCCCCCEE
KGGHVGGSMIHDVLQTTTGEWIFEGPRIDTQATHGTGCTLASAIAALLAQGVSLPEAIER
ECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
ARAYLFGAIQNAQGFGKGHHPVNHGWTLKADD
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
AEAEGPRGRVLIIAGSDSGGGAGIQADIKTVTMLGGFAMTAVTAITVQDTTGVHGVWPV
CCCCCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
PVETVTAQMQVTLADIGADAIKTGMLGTAPLVEAVAEMLEARAFGISRVIDPVMISTSGH
CHHHHHHEEEEEHHHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCC
RLVDERAIGAIRAELVPRARLVTPNAPEAEVLTGKAVDTLDGQRRAAERLLELGAHGALV
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCHHHCCHHHHHHHHHHHHCCCCCEE
KGGHVGGSMIHDVLQTTTGEWIFEGPRIDTQATHGTGCTLASAIAALLAQGVSLPEAIER
ECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
ARAYLFGAIQNAQGFGKGHHPVNHGWTLKADD
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA