Click here to switch to the map view.

The map label for this gene is dusA [H]

Identifier: 114799763

GI number: 114799763

Start: 283755

End: 284741

Strand: Direct

Name: dusA [H]

Synonym: HNE_0293

Alternate gene names: 114799763

Gene position: 283755-284741 (Clockwise)

Preceding gene: 114798239

Following gene: 114799463

Centisome position: 7.66

GC content: 61.8

Gene sequence:

>987_bases
ATGTCTAAAAAACAAGACTATAGATTTTCTGTCGCTCCGATGATGGACTGGACTGACCGGCATTGCCGCGCGTTTCACCG
CACTTTGACCAAGCGCGCGCTTTTGTGGACCGAGATGGTAACGGCCGATGCCGTGATCCATGGCGACCGTCAGCGGCTCA
TCGGTTTCAGCGATGTGGAGCATCCCCTCGTCCTGCAGCTGGGCGGGTCTGAGCCCGGCAAGCTGGCGGAGGCGGCGCGG
ATTGCCGAAGGGTACGGGTATGATGAGGTGAACCTCAATTGTGGCTGCCCGTCAGAGCGGGTCCAGTCGGGCGCCTTCGG
GGCCTGCCTGATGGCCGAGCCTGACCTGGTCGCAGACTGCGTGGCCGCAATGCGGGCGGCGGTGTCCATCCCGGTAACGG
TCAAGAACCGGATCGCGATTGATGACCTGCCGGCACGCGAGACGCTGTTCACCTTCATTGAAAAAGTTTCGGTGGCGGGG
TGTTCTGTATTCACCGTGCATGCGCGCAAAGCCTGGTTGAAGGGGCTGAGCCCCAAGGAAAACCGGGAGGTTCCTCCGCT
GGACTATGGGCTGGTGGCGGAACTCAAGACGGCGCGGCCGGATCTGACGATTATTCTTAATGGCGGCATCGCGACAATTG
ATCAGTGCCGGGATCATATCGGTGTGTTCGACGGCGTGATGCTTGGACGGGCGGCGTATCAGACACCGGGCCTTCTGGGG
CAGGTGGATTCCGATCTGTTTGGGGAAGGGGCAATGATCGCGGCGGGAGACGCGGTGCGCGCCTATGAACCCTATATTGC
GGCGCGCCTGGCCGAAGGCTCGAATCTGCATGCCATGACGCGGCATATGCTCGGACTTTTTGCTGGCCAGCCTGGCGCCC
GCCAGTGGCGGCGTATTCTGTCGGAGCAGGGAGTAAAGAAAGGCGCGGGACTGGACGTGCTGCGCGCTGCGCTCGGCGCG
GTTGAGAACCCTGCGATGGCGGGTTAA

Upstream 100 bases:

>100_bases
TCCGTGTGCACAGAGGGGTTAAATCAGGTACAAACGTGTGCAAATTGGTGCACACAAACTGCACACGTTCTCTCTTTAGT
CCCTTGATTGTACTCAAAAA

Downstream 100 bases:

>100_bases
CCGTGTTCTTTATTTGTTTGATTTCCCATGTTGCCTTTTCCAAGTTGGGAGAGAGGGCGCAGGGGATGGTCTGCCGGGTA
GCAACCTTGGCTTTTGAGGG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSKKQDYRFSVAPMMDWTDRHCRAFHRTLTKRALLWTEMVTADAVIHGDRQRLIGFSDVEHPLVLQLGGSEPGKLAEAAR
IAEGYGYDEVNLNCGCPSERVQSGAFGACLMAEPDLVADCVAAMRAAVSIPVTVKNRIAIDDLPARETLFTFIEKVSVAG
CSVFTVHARKAWLKGLSPKENREVPPLDYGLVAELKTARPDLTIILNGGIATIDQCRDHIGVFDGVMLGRAAYQTPGLLG
QVDSDLFGEGAMIAAGDAVRAYEPYIAARLAEGSNLHAMTRHMLGLFAGQPGARQWRRILSEQGVKKGAGLDVLRAALGA
VENPAMAG

Sequences:

>Translated_328_residues
MSKKQDYRFSVAPMMDWTDRHCRAFHRTLTKRALLWTEMVTADAVIHGDRQRLIGFSDVEHPLVLQLGGSEPGKLAEAAR
IAEGYGYDEVNLNCGCPSERVQSGAFGACLMAEPDLVADCVAAMRAAVSIPVTVKNRIAIDDLPARETLFTFIEKVSVAG
CSVFTVHARKAWLKGLSPKENREVPPLDYGLVAELKTARPDLTIILNGGIATIDQCRDHIGVFDGVMLGRAAYQTPGLLG
QVDSDLFGEGAMIAAGDAVRAYEPYIAARLAEGSNLHAMTRHMLGLFAGQPGARQWRRILSEQGVKKGAGLDVLRAALGA
VENPAMAG
>Mature_327_residues
SKKQDYRFSVAPMMDWTDRHCRAFHRTLTKRALLWTEMVTADAVIHGDRQRLIGFSDVEHPLVLQLGGSEPGKLAEAARI
AEGYGYDEVNLNCGCPSERVQSGAFGACLMAEPDLVADCVAAMRAAVSIPVTVKNRIAIDDLPARETLFTFIEKVSVAGC
SVFTVHARKAWLKGLSPKENREVPPLDYGLVAELKTARPDLTIILNGGIATIDQCRDHIGVFDGVMLGRAAYQTPGLLGQ
VDSDLFGEGAMIAAGDAVRAYEPYIAARLAEGSNLHAMTRHMLGLFAGQPGARQWRRILSEQGVKKGAGLDVLRAALGAV
ENPAMAG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=239, Percent_Identity=29.2887029288703, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=28.099173553719, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=58.2278481012658, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788462, Length=179, Percent_Identity=33.5195530726257, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789660, Length=246, Percent_Identity=28.0487804878049, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=31.4553990610329, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=28.7425149700599, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=287, Percent_Identity=27.1777003484321, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24580595, Length=244, Percent_Identity=27.0491803278689, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920448, Length=244, Percent_Identity=27.0491803278689, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921524, Length=246, Percent_Identity=24.7967479674797, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45549423, Length=206, Percent_Identity=27.1844660194175, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 35386; Mature: 35255

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKQDYRFSVAPMMDWTDRHCRAFHRTLTKRALLWTEMVTADAVIHGDRQRLIGFSDVE
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCCCCC
HPLVLQLGGSEPGKLAEAARIAEGYGYDEVNLNCGCPSERVQSGAFGACLMAEPDLVADC
CCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHCCCCCCEEEECCCHHHHHH
VAAMRAAVSIPVTVKNRIAIDDLPARETLFTFIEKVSVAGCSVFTVHARKAWLKGLSPKE
HHHHHHHHCCCEEECCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCC
NREVPPLDYGLVAELKTARPDLTIILNGGIATIDQCRDHIGVFDGVMLGRAAYQTPGLLG
CCCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QVDSDLFGEGAMIAAGDAVRAYEPYIAARLAEGSNLHAMTRHMLGLFAGQPGARQWRRIL
CCCHHHCCCCCEEECCCCHHHHCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
SEQGVKKGAGLDVLRAALGAVENPAMAG
HHCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SKKQDYRFSVAPMMDWTDRHCRAFHRTLTKRALLWTEMVTADAVIHGDRQRLIGFSDVE
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCCCCC
HPLVLQLGGSEPGKLAEAARIAEGYGYDEVNLNCGCPSERVQSGAFGACLMAEPDLVADC
CCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHCCCCCCEEEECCCHHHHHH
VAAMRAAVSIPVTVKNRIAIDDLPARETLFTFIEKVSVAGCSVFTVHARKAWLKGLSPKE
HHHHHHHHCCCEEECCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCC
NREVPPLDYGLVAELKTARPDLTIILNGGIATIDQCRDHIGVFDGVMLGRAAYQTPGLLG
CCCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QVDSDLFGEGAMIAAGDAVRAYEPYIAARLAEGSNLHAMTRHMLGLFAGQPGARQWRRIL
CCCHHHCCCCCEEECCCCHHHHCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
SEQGVKKGAGLDVLRAALGAVENPAMAG
HHCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]