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The map label for this gene is barA [H]

Identifier: 114799463

GI number: 114799463

Start: 284928

End: 286418

Strand: Direct

Name: barA [H]

Synonym: HNE_0294

Alternate gene names: 114799463

Gene position: 284928-286418 (Clockwise)

Preceding gene: 114799763

Following gene: 114800449

Centisome position: 7.69

GC content: 65.73

Gene sequence:

>1491_bases
ATGACCCATGACCCCGAACGCCCCGATGGCGCAGAAACGCCCGAACAGACATTTCTCGCCACGGCGAGTCATGAAATACG
CACGCCTCTGAACGGTATTCTCGGTACGGTTTCGCTGTTGCTGGAAACAGAGCTTTCGCCGGCTCAGCGCGAATATGCCG
AGACGATCCGCCTGTCAGGCGGGCGCCTGTTGGACCTGCTCAATAACGTTCTCGATTATGCCCGGTTGGACGCCTCTGCC
GTCGAAGTCGAGGCCGAGAATTTCTGCCCCGTCCGGCTGTGCGGGGAAGTAGTCGAACTTCTCAGTCCCCGCGCGCATGC
GGCCGGGCTTGATCTGGCGGTACGTTCCCTGCCGTCGCCGATGCCGGGATTTCTGGGAGACGCCGGCCGTATAAGGCAGA
TCCTGTTCAATCTTGTCGGTAATGCGCTGAAGTTCACCCCGCGCGGCGCTGTCCTTATCGATGCTGAAGTCGTTACAGGC
GGCCTCGCTTTTCATATTCGCGATACCGGGCCGGGCATTGCGCCCGATGACCAGGCGCGCCTGTTCGAAGCGTTTCGCCA
GACTGGCGCCGGGGATGCTTACCGCGATGGCGGCGTTGGTCTCGGCCTAGCCATCGTCAAGCGTCTGACCGATCTGCTCG
GCGGGCGGATTGATGTCGTCTCAGCGCTCGGGGAAGGGGCCTCATTCAGCGTGTTCCTGCCACTGCAGCAATCTGGCGCT
CCGCCGGCGGACGCGGTGGCGTTCATAGGCGGCCGCGTCGGTCTCGCGGGGCTGCCGCCCGCAACGGCGCTCTCCCTCTC
GGCCGGCCTCCAGGCTATGGGCGCGTCTGTCCTGCAGATGGATGTGGCGAGCGGACAGATACCTGACGGAATAGACGTCC
TGCTGGTGGGGGCAGACCTGCCCGAGGGCGCGGTTGCGGCGATGGCGCGCCAGGCGACATCACTGGTTGTTCTGCGGCCC
GAAGATCGGGGCGCCATTGCCCGGTTCCGGGCACTGGGCTGTGTCGGTTGGCTCGTCCGGCCGCTGCGTATGAGTTCGGT
GGCCGAGCGTATCCAGCTAGCCCGCTCCGGTGGGGATGCCGCCGACGAGCAGGAACTGGCGGCGGGCGCGGGCCGCGTGC
TGATTGCCGATGACAATCCCGTAAACGCGCTGATTGCGCGCCGCGCCTTGGAATCGGCCGGATTTACCGTAACAGTTGCC
GCAACGGGCAGCGAAGCGCTTGAGGCTGCCGCCAGAATGGAGCCGAACCTTGTGCTGATGGATCTGCGAATGCCGGTCAT
GGACGGGTTTGAAGCGATGCGCCGGTTGCGGGCTGGCGGGTTTTCGCCGCCCATCATTGCAGTCTCGGCAGAGATCAATC
CGGACATCGAACGCCGGGCACGCGCCGCAGGCGCAGACGGCGTCGCAGCAAAGCCGTTGGACGCCGAGGCCCTGCGCCGG
CTGGCGCTGCGCTGGACCGGCCGGTCCGGCGCTGTGGCGGGCGCCGCATGA

Upstream 100 bases:

>100_bases
TTGGCTTTTGAGGGCGCACTTGCGCGATAGTCCATTATATCCTTGGGCCGGCGGGAGCAGTTAAGCCTCTTCTTAAGGCT
TTCAGCTAGACTCTTGACCC

Downstream 100 bases:

>100_bases
CGGATACGGTTGGTGCGGCAGAGGCCAAAAAGACGCCGCGTTTCATCCGGGCGCTGCGTGCGCTGAACGACCGGCGCATG
GCGGCGATGCTGCTGCTCTC

Product: sensor histidine kinase/response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTHDPERPDGAETPEQTFLATASHEIRTPLNGILGTVSLLLETELSPAQREYAETIRLSGGRLLDLLNNVLDYARLDASA
VEVEAENFCPVRLCGEVVELLSPRAHAAGLDLAVRSLPSPMPGFLGDAGRIRQILFNLVGNALKFTPRGAVLIDAEVVTG
GLAFHIRDTGPGIAPDDQARLFEAFRQTGAGDAYRDGGVGLGLAIVKRLTDLLGGRIDVVSALGEGASFSVFLPLQQSGA
PPADAVAFIGGRVGLAGLPPATALSLSAGLQAMGASVLQMDVASGQIPDGIDVLLVGADLPEGAVAAMARQATSLVVLRP
EDRGAIARFRALGCVGWLVRPLRMSSVAERIQLARSGGDAADEQELAAGAGRVLIADDNPVNALIARRALESAGFTVTVA
ATGSEALEAAARMEPNLVLMDLRMPVMDGFEAMRRLRAGGFSPPIIAVSAEINPDIERRARAAGADGVAAKPLDAEALRR
LALRWTGRSGAVAGAA

Sequences:

>Translated_496_residues
MTHDPERPDGAETPEQTFLATASHEIRTPLNGILGTVSLLLETELSPAQREYAETIRLSGGRLLDLLNNVLDYARLDASA
VEVEAENFCPVRLCGEVVELLSPRAHAAGLDLAVRSLPSPMPGFLGDAGRIRQILFNLVGNALKFTPRGAVLIDAEVVTG
GLAFHIRDTGPGIAPDDQARLFEAFRQTGAGDAYRDGGVGLGLAIVKRLTDLLGGRIDVVSALGEGASFSVFLPLQQSGA
PPADAVAFIGGRVGLAGLPPATALSLSAGLQAMGASVLQMDVASGQIPDGIDVLLVGADLPEGAVAAMARQATSLVVLRP
EDRGAIARFRALGCVGWLVRPLRMSSVAERIQLARSGGDAADEQELAAGAGRVLIADDNPVNALIARRALESAGFTVTVA
ATGSEALEAAARMEPNLVLMDLRMPVMDGFEAMRRLRAGGFSPPIIAVSAEINPDIERRARAAGADGVAAKPLDAEALRR
LALRWTGRSGAVAGAA
>Mature_495_residues
THDPERPDGAETPEQTFLATASHEIRTPLNGILGTVSLLLETELSPAQREYAETIRLSGGRLLDLLNNVLDYARLDASAV
EVEAENFCPVRLCGEVVELLSPRAHAAGLDLAVRSLPSPMPGFLGDAGRIRQILFNLVGNALKFTPRGAVLIDAEVVTGG
LAFHIRDTGPGIAPDDQARLFEAFRQTGAGDAYRDGGVGLGLAIVKRLTDLLGGRIDVVSALGEGASFSVFLPLQQSGAP
PADAVAFIGGRVGLAGLPPATALSLSAGLQAMGASVLQMDVASGQIPDGIDVLLVGADLPEGAVAAMARQATSLVVLRPE
DRGAIARFRALGCVGWLVRPLRMSSVAERIQLARSGGDAADEQELAAGAGRVLIADDNPVNALIARRALESAGFTVTVAA
TGSEALEAAARMEPNLVLMDLRMPVMDGFEAMRRLRAGGFSPPIIAVSAEINPDIERRARAAGADGVAAKPLDAEALRRL
ALRWTGRSGAVAGAA

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=501, Percent_Identity=32.5349301397206, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI145693157, Length=481, Percent_Identity=28.6902286902287, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081816, Length=319, Percent_Identity=34.4827586206897, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI48994928, Length=218, Percent_Identity=34.4036697247706, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788713, Length=236, Percent_Identity=32.2033898305085, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=31.6964285714286, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786912, Length=230, Percent_Identity=32.6086956521739, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790346, Length=226, Percent_Identity=28.7610619469027, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=27.2321428571429, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=27.3127753303965, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790300, Length=232, Percent_Identity=29.3103448275862, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786783, Length=245, Percent_Identity=26.1224489795918, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=29.9559471365639, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082128, Length=237, Percent_Identity=27.4261603375527, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790551, Length=210, Percent_Identity=29.5238095238095, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787894, Length=221, Percent_Identity=29.8642533936652, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=33.8983050847458, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=132, Percent_Identity=34.0909090909091, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51519; Mature: 51387

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDPERPDGAETPEQTFLATASHEIRTPLNGILGTVSLLLETELSPAQREYAETIRLSG
CCCCCCCCCCCCCCHHHEEEECCHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GRLLDLLNNVLDYARLDASAVEVEAENFCPVRLCGEVVELLSPRAHAAGLDLAVRSLPSP
CHHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
MPGFLGDAGRIRQILFNLVGNALKFTPRGAVLIDAEVVTGGLAFHIRDTGPGIAPDDQAR
CCCCCCCHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEECCEEEEEECCCCCCCCCHHHH
LFEAFRQTGAGDAYRDGGVGLGLAIVKRLTDLLGGRIDVVSALGEGASFSVFLPLQQSGA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCC
PPADAVAFIGGRVGLAGLPPATALSLSAGLQAMGASVLQMDVASGQIPDGIDVLLVGADL
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCEEEEEECCC
PEGAVAAMARQATSLVVLRPEDRGAIARFRALGCVGWLVRPLRMSSVAERIQLARSGGDA
CCHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADEQELAAGAGRVLIADDNPVNALIARRALESAGFTVTVAATGSEALEAAARMEPNLVLM
CCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEEE
DLRMPVMDGFEAMRRLRAGGFSPPIIAVSAEINPDIERRARAAGADGVAAKPLDAEALRR
ECCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHH
LALRWTGRSGAVAGAA
HHHHCCCCCCCCCCCC
>Mature Secondary Structure 
THDPERPDGAETPEQTFLATASHEIRTPLNGILGTVSLLLETELSPAQREYAETIRLSG
CCCCCCCCCCCCCHHHEEEECCHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GRLLDLLNNVLDYARLDASAVEVEAENFCPVRLCGEVVELLSPRAHAAGLDLAVRSLPSP
CHHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
MPGFLGDAGRIRQILFNLVGNALKFTPRGAVLIDAEVVTGGLAFHIRDTGPGIAPDDQAR
CCCCCCCHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEECCEEEEEECCCCCCCCCHHHH
LFEAFRQTGAGDAYRDGGVGLGLAIVKRLTDLLGGRIDVVSALGEGASFSVFLPLQQSGA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCC
PPADAVAFIGGRVGLAGLPPATALSLSAGLQAMGASVLQMDVASGQIPDGIDVLLVGADL
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCEEEEEECCC
PEGAVAAMARQATSLVVLRPEDRGAIARFRALGCVGWLVRPLRMSSVAERIQLARSGGDA
CCHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADEQELAAGAGRVLIADDNPVNALIARRALESAGFTVTVAATGSEALEAAARMEPNLVLM
CCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEEE
DLRMPVMDGFEAMRRLRAGGFSPPIIAVSAEINPDIERRARAAGADGVAAKPLDAEALRR
ECCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHH
LALRWTGRSGAVAGAA
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]