Click here to switch to the map view.

The map label for this gene is exsB

Identifier: 114799592

GI number: 114799592

Start: 527263

End: 527982

Strand: Direct

Name: exsB

Synonym: HNE_0531

Alternate gene names: 114799592

Gene position: 527263-527982 (Clockwise)

Preceding gene: 114798658

Following gene: 114798300

Centisome position: 14.23

GC content: 65.83

Gene sequence:

>720_bases
ATGACACAGCCAATTTCCTCCGCGCTCGTCCTGTTTTCCGGGGGACAGGATTCGGCCACCTGCCTGGCCTGGGCCCTCGA
CCGGTTCGACCGGGTGGAGACGATCGGCTTCGATTATGGCCAGCGCCACGCCATCGAACTCGCCTGCCGGCCGAAAGTGC
GCGCCGCGATTCGCCAGATGAGCCCTGTCTGGGCGGAAAAGCTGGGCGAGGATCACATGATCGATGCTGGCGTCCTCAAA
TCCCTCGGCGCCACCGCGATGACCCACGAGGTCGAGATCAGCATGACCGCCGCTGGCCTGCCCAACACGTTCGTGCCGGG
AAGAAACCTTCTCTTCTTTACCCTGGCCGGCGCGCTTGCCGTGCGCCGGGGCATCGGCGTGCTGGTCGGCGGCATGTGCG
AAACCGACTATTCGGGCTATCCTGACTGCCGCGCCGATACGCTCAAAGCCCAGGAGCAGACCCTCCGCCTTGGTCTCGAC
GCCGACATCCGCATCGATGCGCCCCTGATGTATCTCGACAAGGCCGCCACCTGGCAGCTCGCCCAGGAACTCGGCGGAGA
CGCGCTGGTTGCCCTGATCGGCGAAGAAACCCACACCTGTTATCTGGGCGAGCGCGGCGCGCGCCATGACTGGGGCTATG
GCTGCGGCACCTGCCCGGCCTGCGAACTCAGGTCAAATGGCTGGCAGCGCTGGCAGGATGGCAAGGCGGCCGCCCCATGA

Upstream 100 bases:

>100_bases
CCCTACGTGAGGTCGAAACTATTTGTGTCGGTGTCAAAAATGTGACGCCTTCCGCAACGTAGCCTTGGCCGGAACCGGCC
GCGCTGATAGAGCGGCGCGC

Downstream 100 bases:

>100_bases
CCTATTCTGTCAAAGAGGCCTTCTATACCCTGCAGGGCGAAGGCGCCCATACCGGCCGCGCGGCGGTCTTCCTGCGCTTT
GCCGGCTGCAACCTCTGGAG

Product: exsB protein

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTQPISSALVLFSGGQDSATCLAWALDRFDRVETIGFDYGQRHAIELACRPKVRAAIRQMSPVWAEKLGEDHMIDAGVLK
SLGATAMTHEVEISMTAAGLPNTFVPGRNLLFFTLAGALAVRRGIGVLVGGMCETDYSGYPDCRADTLKAQEQTLRLGLD
ADIRIDAPLMYLDKAATWQLAQELGGDALVALIGEETHTCYLGERGARHDWGYGCGTCPACELRSNGWQRWQDGKAAAP

Sequences:

>Translated_239_residues
MTQPISSALVLFSGGQDSATCLAWALDRFDRVETIGFDYGQRHAIELACRPKVRAAIRQMSPVWAEKLGEDHMIDAGVLK
SLGATAMTHEVEISMTAAGLPNTFVPGRNLLFFTLAGALAVRRGIGVLVGGMCETDYSGYPDCRADTLKAQEQTLRLGLD
ADIRIDAPLMYLDKAATWQLAQELGGDALVALIGEETHTCYLGERGARHDWGYGCGTCPACELRSNGWQRWQDGKAAAP
>Mature_238_residues
TQPISSALVLFSGGQDSATCLAWALDRFDRVETIGFDYGQRHAIELACRPKVRAAIRQMSPVWAEKLGEDHMIDAGVLKS
LGATAMTHEVEISMTAAGLPNTFVPGRNLLFFTLAGALAVRRGIGVLVGGMCETDYSGYPDCRADTLKAQEQTLRLGLDA
DIRIDAPLMYLDKAATWQLAQELGGDALVALIGEETHTCYLGERGARHDWGYGCGTCPACELRSNGWQRWQDGKAAAP

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=233, Percent_Identity=41.6309012875536, Blast_Score=176, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_HYPNA (Q0C4T2)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759261.1
- ProteinModelPortal:   Q0C4T2
- SMR:   Q0C4T2
- STRING:   Q0C4T2
- GeneID:   4289088
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0531
- NMPDR:   fig|228405.5.peg.523
- TIGR:   HNE_0531
- eggNOG:   COG0603
- HOGENOM:   HBG553284
- OMA:   CETDYSG
- PhylomeDB:   Q0C4T2
- ProtClustDB:   CLSK864419
- BioCyc:   HNEP81032:HNE_0531-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 25864; Mature: 25733

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPISSALVLFSGGQDSATCLAWALDRFDRVETIGFDYGQRHAIELACRPKVRAAIRQM
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHC
SPVWAEKLGEDHMIDAGVLKSLGATAMTHEVEISMTAAGLPNTFVPGRNLLFFTLAGALA
CHHHHHHCCCCCCCCHHHHHHHCCHHEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
VRRGIGVLVGGMCETDYSGYPDCRADTLKAQEQTLRLGLDADIRIDAPLMYLDKAATWQL
HHHCCCEEECCEECCCCCCCCCCCHHHHHHHHHHHEECCCCCEEECCCHHHHHHHHHHHH
AQELGGDALVALIGEETHTCYLGERGARHDWGYGCGTCPACELRSNGWQRWQDGKAAAP
HHHCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCHHCCCCCCCCC
>Mature Secondary Structure 
TQPISSALVLFSGGQDSATCLAWALDRFDRVETIGFDYGQRHAIELACRPKVRAAIRQM
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHC
SPVWAEKLGEDHMIDAGVLKSLGATAMTHEVEISMTAAGLPNTFVPGRNLLFFTLAGALA
CHHHHHHCCCCCCCCHHHHHHHCCHHEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
VRRGIGVLVGGMCETDYSGYPDCRADTLKAQEQTLRLGLDADIRIDAPLMYLDKAATWQL
HHHCCCEEECCEECCCCCCCCCCCHHHHHHHHHHHEECCCCCEEECCCHHHHHHHHHHHH
AQELGGDALVALIGEETHTCYLGERGARHDWGYGCGTCPACELRSNGWQRWQDGKAAAP
HHHCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA