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The map label for this gene is amiE [H]

Identifier: 114799416

GI number: 114799416

Start: 1120494

End: 1121624

Strand: Reverse

Name: amiE [H]

Synonym: HNE_1113

Alternate gene names: 114799416

Gene position: 1121624-1120494 (Counterclockwise)

Preceding gene: 114799839

Following gene: 114798258

Centisome position: 30.27

GC content: 62.42

Gene sequence:

>1131_bases
ATGACCCGCCGAGGCCTGCTTCTGGGCGCTGCCGCCACATCCCTTCTGCCGGCCTTCGGCAGCTCAAACGCCCATGCCGG
AGAGGCAAAATCCATGCGCTACACCGCCCTCGCGCTGCAGACCGTCTGCGAAGCCGTCAACCAGGACAAGACCGCCGAAG
CCTCCCATGCCCGGATGAATGCCGCCATCACCCGCGTCACCGGTCAGATCATGGCGTCCAGGGGTTTCCTCAAAGGCTTC
AACGGCTACGACCTGAAACTCGTCGTTCTGCCGGAATACTGGATGACCGGTTTCCCGATGGGCGAAACGCGCGAGACCTG
GCAGGCCAAGGCCGCCATTCCGATGGACGGCGCCTTCCCCGATGCGCTGGCAAAGATGGCCCAGACCGCCGGCGTCTATC
TGTGCTCGAACCATTACGAAACCGACCCGAACTTCCCGGAACTCTACTTCCAGGCCAACGTCATCTACGGGCCAACCGGT
GACACCCTCCTGCGCTATCGCCGCATGATCTCGCTCTACTCGCCCTCTCCGTATGATGTATGGGACAAGTATCTCGACAT
CTATGGCGCCGATGCGATCTTCCCGGTGGCCGAAACCGAAATCGGCGCACTGGCCACCATCGCCAGCGAAGAAATCCTCT
ACCCCGAAATCGCCCGCATGCACGCTTTCAAGGGCGCAGAAATCCTGCTTCATCCGACGTCGGAAGTCGGCAGCCCCAGC
CTTACACCCAAACATATCGCCAAGAAAGCCCGGGCCATCGAGAACATCTCCTATGTCGTCTCGGCCAACTCCGCGAGCAT
CACGGGCACGCCGGTTCCCGCTGCCTCTACGGATGGCATGTCCATCGTGCTCGACTGGTATGGCCGCACGCTGGCTGAGG
CCGGCACCGGTGAGACGATGAACGCCAACGCTGTGCTTGATCTCACCGCCTTGCGCGAAACACGCCGCAAGACCGGCATG
ACCAACACGCTCTCGCGGCTCCCTCTGGCCGCCTTCGCCCCGGCTTATTCCGGGAACGAACTGGCCCCAGCCAACCGCAC
TCCCGATGGCAAGATGGTCCAGCGCGCCGATGCGATTGCACAGCAGCAGGCCGTCATTGACCGGCTGGTCTCGGCAGGGG
TTCTGAAATAG

Upstream 100 bases:

>100_bases
TCGAAGCGGTTACACGCGTGCTCAAAGGGCTCGCTGCCATGGACGGCCTCGAAGTGGCGACCGCCGCAAGCGTCACATCC
GCCTTTGAAGGAGCCGCCCC

Downstream 100 bases:

>100_bases
GCCGTTGGCGAAAGGCCGGTTACTGCCCCCAGCCCGGCCCGCCTTCGCCCTTCTGCAGCTTACGGACTTCTTCCAGGCTT
TCGGGCGGCGCCAGCCAGAG

Product: carbon-nitrogen family hydrolase

Products: NA

Alternate protein names: Acylamide amidohydrolase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTRRGLLLGAAATSLLPAFGSSNAHAGEAKSMRYTALALQTVCEAVNQDKTAEASHARMNAAITRVTGQIMASRGFLKGF
NGYDLKLVVLPEYWMTGFPMGETRETWQAKAAIPMDGAFPDALAKMAQTAGVYLCSNHYETDPNFPELYFQANVIYGPTG
DTLLRYRRMISLYSPSPYDVWDKYLDIYGADAIFPVAETEIGALATIASEEILYPEIARMHAFKGAEILLHPTSEVGSPS
LTPKHIAKKARAIENISYVVSANSASITGTPVPAASTDGMSIVLDWYGRTLAEAGTGETMNANAVLDLTALRETRRKTGM
TNTLSRLPLAAFAPAYSGNELAPANRTPDGKMVQRADAIAQQQAVIDRLVSAGVLK

Sequences:

>Translated_376_residues
MTRRGLLLGAAATSLLPAFGSSNAHAGEAKSMRYTALALQTVCEAVNQDKTAEASHARMNAAITRVTGQIMASRGFLKGF
NGYDLKLVVLPEYWMTGFPMGETRETWQAKAAIPMDGAFPDALAKMAQTAGVYLCSNHYETDPNFPELYFQANVIYGPTG
DTLLRYRRMISLYSPSPYDVWDKYLDIYGADAIFPVAETEIGALATIASEEILYPEIARMHAFKGAEILLHPTSEVGSPS
LTPKHIAKKARAIENISYVVSANSASITGTPVPAASTDGMSIVLDWYGRTLAEAGTGETMNANAVLDLTALRETRRKTGM
TNTLSRLPLAAFAPAYSGNELAPANRTPDGKMVQRADAIAQQQAVIDRLVSAGVLK
>Mature_375_residues
TRRGLLLGAAATSLLPAFGSSNAHAGEAKSMRYTALALQTVCEAVNQDKTAEASHARMNAAITRVTGQIMASRGFLKGFN
GYDLKLVVLPEYWMTGFPMGETRETWQAKAAIPMDGAFPDALAKMAQTAGVYLCSNHYETDPNFPELYFQANVIYGPTGD
TLLRYRRMISLYSPSPYDVWDKYLDIYGADAIFPVAETEIGALATIASEEILYPEIARMHAFKGAEILLHPTSEVGSPSL
TPKHIAKKARAIENISYVVSANSASITGTPVPAASTDGMSIVLDWYGRTLAEAGTGETMNANAVLDLTALRETRRKTGMT
NTLSRLPLAAFAPAYSGNELAPANRTPDGKMVQRADAIAQQQAVIDRLVSAGVLK

Specific function: Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 40481; Mature: 40349

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRGLLLGAAATSLLPAFGSSNAHAGEAKSMRYTALALQTVCEAVNQDKTAEASHARMN
CCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AAITRVTGQIMASRGFLKGFNGYDLKLVVLPEYWMTGFPMGETRETWQAKAAIPMDGAFP
HHHHHHHHHHHHHCCHHCCCCCCCEEEEEECHHHHCCCCCCCCCHHHHHCCCCCCCCCCH
DALAKMAQTAGVYLCSNHYETDPNFPELYFQANVIYGPTGDTLLRYRRMISLYSPSPYDV
HHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHH
WDKYLDIYGADAIFPVAETEIGALATIASEEILYPEIARMHAFKGAEILLHPTSEVGSPS
HHHHHHHHCCCEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCC
LTPKHIAKKARAIENISYVVSANSASITGTPVPAASTDGMSIVLDWYGRTLAEAGTGETM
CCHHHHHHHHHHHHCCEEEEECCCCEECCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCC
NANAVLDLTALRETRRKTGMTNTLSRLPLAAFAPAYSGNELAPANRTPDGKMVQRADAIA
CCCEEEEHHHHHHHHHHCCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
QQQAVIDRLVSAGVLK
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRRGLLLGAAATSLLPAFGSSNAHAGEAKSMRYTALALQTVCEAVNQDKTAEASHARMN
CCCCEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AAITRVTGQIMASRGFLKGFNGYDLKLVVLPEYWMTGFPMGETRETWQAKAAIPMDGAFP
HHHHHHHHHHHHHCCHHCCCCCCCEEEEEECHHHHCCCCCCCCCHHHHHCCCCCCCCCCH
DALAKMAQTAGVYLCSNHYETDPNFPELYFQANVIYGPTGDTLLRYRRMISLYSPSPYDV
HHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHH
WDKYLDIYGADAIFPVAETEIGALATIASEEILYPEIARMHAFKGAEILLHPTSEVGSPS
HHHHHHHHCCCEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCC
LTPKHIAKKARAIENISYVVSANSASITGTPVPAASTDGMSIVLDWYGRTLAEAGTGETM
CCHHHHHHHHHHHHCCEEEEECCCCEECCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCC
NANAVLDLTALRETRRKTGMTNTLSRLPLAAFAPAYSGNELAPANRTPDGKMVQRADAIA
CCCEEEEHHHHHHHHHHCCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
QQQAVIDRLVSAGVLK
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA