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The map label for this gene is ksgA [H]

Identifier: 114799371

GI number: 114799371

Start: 2241239

End: 2242096

Strand: Direct

Name: ksgA [H]

Synonym: HNE_2152

Alternate gene names: 114799371

Gene position: 2241239-2242096 (Clockwise)

Preceding gene: 114798861

Following gene: 114800162

Centisome position: 60.49

GC content: 64.1

Gene sequence:

>858_bases
ATGAACAACGACCATGACTATGGCGACGACAACGGCAGTCACCCGGCGCTGACCGATGCCCCCGCCCGCAAGGCGCTGGG
CCAGCACTTTCTGTTCGATCCCAGCATCCTGAAACGCGCCGCAAATGCTGCTGGCCCGCTCAAGGGCAAGACGGTCATCG
AAGTCGGCCCCGGCCCTGGCGGTCTGACGCGCGCCATCCTGAACGAAGAACCGGCCCTGCTTATCGCGGTGGAGACCGAC
CCACGCTTTTCCGAAGCGCTGATGAGTTGGCCGGAGGCTAAAAACGGCCGCCTGCAGGTGATCGCCAGAGATGCCCGCAA
GGTGCATTGGGAGAAGGTGTTGCAGGAAGCGGGCGCCGCAACGCCCGTGATGATCATTGCCAACCTTCCCTACAATGTCG
GCACGCCGCTGCTCATCGACTGGCTGAAAGCCGGTGACTGGCGCGGCCCCATGGCGCTGATGTTCCAGCGGGAAGTGGCC
GAGCGGATCTGCGCGCAGCCCGATACCGATGCCTATGGCCGCCTGGCTGTCATCTCGCAGGCAGTGACACGTCCCCGTAT
TGCCTTCACTCTGCCGCCAGGCGCGTTCCGGCCGCCGCCGAAGGTTGATAGCGCTGTCGTCGAGTTTGAACCACTGCCGC
CGGGCGAGCGCTTCGAGCACCTTGCGCTGCTGGAACAGATTGCCGGCGCCGCCTTTGGCCAGCGCCGCAAGATGCTGCGC
GCCGCGTTGAAACCCTTCGCGAAAAAGCGCGGGATGAAAGCCGAAGCCTGGCTGGAAGATTGCGGCATCAAGCCGACCGC
GCGCGCCGAAACGCTTACACAGGCGGAATTCCGCAAACTGGCAACAAGCCTGGCTTAA

Upstream 100 bases:

>100_bases
CCTATGACGCAGCCGCCGCCGGAACGGCCAAGGCCGATAGCCTGATCGCCGCCATTCATTCTGCGCGGGAACAAGCCGCC
AATTGGGCGAGGAGCCCCAC

Downstream 100 bases:

>100_bases
GCTTCGCCCAGCAGGGCGCGCACGTGATCTTCCAGATCAATCTGGCGCAGGCGCTTCAGCCGCTCGACCAGCAGGATGCG
CTTGAGGCGTTGGTAGGCGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNNDHDYGDDNGSHPALTDAPARKALGQHFLFDPSILKRAANAAGPLKGKTVIEVGPGPGGLTRAILNEEPALLIAVETD
PRFSEALMSWPEAKNGRLQVIARDARKVHWEKVLQEAGAATPVMIIANLPYNVGTPLLIDWLKAGDWRGPMALMFQREVA
ERICAQPDTDAYGRLAVISQAVTRPRIAFTLPPGAFRPPPKVDSAVVEFEPLPPGERFEHLALLEQIAGAAFGQRRKMLR
AALKPFAKKRGMKAEAWLEDCGIKPTARAETLTQAEFRKLATSLA

Sequences:

>Translated_285_residues
MNNDHDYGDDNGSHPALTDAPARKALGQHFLFDPSILKRAANAAGPLKGKTVIEVGPGPGGLTRAILNEEPALLIAVETD
PRFSEALMSWPEAKNGRLQVIARDARKVHWEKVLQEAGAATPVMIIANLPYNVGTPLLIDWLKAGDWRGPMALMFQREVA
ERICAQPDTDAYGRLAVISQAVTRPRIAFTLPPGAFRPPPKVDSAVVEFEPLPPGERFEHLALLEQIAGAAFGQRRKMLR
AALKPFAKKRGMKAEAWLEDCGIKPTARAETLTQAEFRKLATSLA
>Mature_285_residues
MNNDHDYGDDNGSHPALTDAPARKALGQHFLFDPSILKRAANAAGPLKGKTVIEVGPGPGGLTRAILNEEPALLIAVETD
PRFSEALMSWPEAKNGRLQVIARDARKVHWEKVLQEAGAATPVMIIANLPYNVGTPLLIDWLKAGDWRGPMALMFQREVA
ERICAQPDTDAYGRLAVISQAVTRPRIAFTLPPGAFRPPPKVDSAVVEFEPLPPGERFEHLALLEQIAGAAFGQRRKMLR
AALKPFAKKRGMKAEAWLEDCGIKPTARAETLTQAEFRKLATSLA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=276, Percent_Identity=36.5942028985507, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI7657198, Length=265, Percent_Identity=30.188679245283, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1786236, Length=267, Percent_Identity=33.7078651685393, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25141369, Length=281, Percent_Identity=32.3843416370107, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25146882, Length=213, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=191, Percent_Identity=31.413612565445, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357273, Length=274, Percent_Identity=35.036496350365, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21358017, Length=227, Percent_Identity=33.920704845815, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31076; Mature: 31076

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDHDYGDDNGSHPALTDAPARKALGQHFLFDPSILKRAANAAGPLKGKTVIEVGPGPG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCC
GLTRAILNEEPALLIAVETDPRFSEALMSWPEAKNGRLQVIARDARKVHWEKVLQEAGAA
HHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCC
TPVMIIANLPYNVGTPLLIDWLKAGDWRGPMALMFQREVAERICAQPDTDAYGRLAVISQ
CCEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AVTRPRIAFTLPPGAFRPPPKVDSAVVEFEPLPPGERFEHLALLEQIAGAAFGQRRKMLR
HHCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALKPFAKKRGMKAEAWLEDCGIKPTARAETLTQAEFRKLATSLA
HHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNDHDYGDDNGSHPALTDAPARKALGQHFLFDPSILKRAANAAGPLKGKTVIEVGPGPG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCC
GLTRAILNEEPALLIAVETDPRFSEALMSWPEAKNGRLQVIARDARKVHWEKVLQEAGAA
HHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCC
TPVMIIANLPYNVGTPLLIDWLKAGDWRGPMALMFQREVAERICAQPDTDAYGRLAVISQ
CCEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AVTRPRIAFTLPPGAFRPPPKVDSAVVEFEPLPPGERFEHLALLEQIAGAAFGQRRKMLR
HHCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALKPFAKKRGMKAEAWLEDCGIKPTARAETLTQAEFRKLATSLA
HHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA