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The map label for this gene is dacF [H]

Identifier: 114799142

GI number: 114799142

Start: 1034657

End: 1035985

Strand: Reverse

Name: dacF [H]

Synonym: HNE_1025

Alternate gene names: 114799142

Gene position: 1035985-1034657 (Counterclockwise)

Preceding gene: 114799201

Following gene: 114797510

Centisome position: 27.96

GC content: 64.03

Gene sequence:

>1329_bases
ATGGCAGCAAAAGTCTGGTCTCGTGCGTTTCGCGGGTCGGCCATCGCAGCCTCAGTATCGATCACCGCCGCCGTCATGGC
GCCGTCTGCGCATGCTGAGAAATACGCCGCAATCGTTGTGGATGCTGATAGCGGAGAGGTTCTTCACGCCCGCAATCAGG
ATGACCCGCGCTACCCGGCCTCGCTGACCAAAGTGATGACGCTCTACCTGCTGTTCGATGCCATCGACGCGGGCGATGTG
AGCCTCAAGGACAAGATCAAGGTTTCGCGCAATGCTGCGGCCCAGCCGCCCTCGAACCTGCGTCTGAGAGCGGGCGATAC
GATTACCGTCGAGGATGCGATCTACGCCCTCACCACCAAATCCGCCAATGACGTGGCCGCTGCCGTTGCCGAGTTCCTCG
GCGGGTCGGAGCGCAAGTTCGCCGCGATGATGACCGACAAGGCCCGCGATCTCGGCCTGTCCAACACCACGTTCCGCAAT
GCGTCGGGCCTGCCGGACACCGCGCAGCTGTCGACGGCGCGCGATCTGGCGATCCTGGCTGACGCCCTGCTCGACAATCA
CGAGCCCTATTACCACTACTTCCAGAACCAGCGCTTCAGCTGGGGCGGGATGACCTACAAGAGCCACAACAACCTCATCG
GCAGCGTCGATGGCGTGGACGGCATCAAGACCGGCTACACCCGCGCATCGGGCTTCAACCTGATGACCTCGGCTGAGCGC
GATGGCCGCCGCGTGATCGCGATCGTGCTGGGCGGAGCTTCGGCGAAGTCGCGCGACGCGCATGTGACCGAGCTCGTCGA
GTCCGCTTTCGTCTCGATTGGACAGACGCCGGACCTGATCGATCCGAACACGAAGCTGATGTCGTTTGCGGCGCTCGATA
CGCCGCTCAACCCCAATGCCGCCGCAGAGCCAATGCTCAATGGCCGGCCCCTGAGCGCGATCCTTGCCGAACAGGCCGGC
ACACAGCCTGCCGAAGTTCTGCAAGAGGCGGAAATCGCCGAAGGCGACGCGGCCGAGCAGGCTCCGACCCTGGAAGTGGT
GCCGGAAATTGCCAACACGCCCAAGACTGTCGCTCCAGAGCCCAAGGCCCCGGAATCCAAAGTGGCAGAGTATAAGGCCC
CTGATTACAAGGTGCCCGACTCGCTGACCGTTTCGGGCTATCAGCAGGCACAGACCGGCGCCGCCGCCAAAGCGCCGCAA
GCCCTCCCGGCTGCTGCCGCTGCCGCGGACACCATCTCGGTCTCTGAATATGAGGCGAGCCAGGCTGCGAAAGCTGCGGC
TGCCAAGGCGGTCATGGACTACACCCGCCGTCAGCTGCGCCGCCGCTGA

Upstream 100 bases:

>100_bases
ACCAGTCATCCTGCGTTAAGCTGTGGTCAATAGAATTAGCGGAATCTAGGGTGAGCCTGGAAAGTCCGGGGGGCGTGTTA
AGTTCTGGAGGGTGCAGCCA

Downstream 100 bases:

>100_bases
CGGCGCTGACGGGCTAACGGCCAGCTTGGGGCCCGGCCTGGGCTCTGGCCGGCAGGGCTTGATGCCGCCTTCTAATTCTA
GGACAAGGCACCAGCGCCAG

Product: putative D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MAAKVWSRAFRGSAIAASVSITAAVMAPSAHAEKYAAIVVDADSGEVLHARNQDDPRYPASLTKVMTLYLLFDAIDAGDV
SLKDKIKVSRNAAAQPPSNLRLRAGDTITVEDAIYALTTKSANDVAAAVAEFLGGSERKFAAMMTDKARDLGLSNTTFRN
ASGLPDTAQLSTARDLAILADALLDNHEPYYHYFQNQRFSWGGMTYKSHNNLIGSVDGVDGIKTGYTRASGFNLMTSAER
DGRRVIAIVLGGASAKSRDAHVTELVESAFVSIGQTPDLIDPNTKLMSFAALDTPLNPNAAAEPMLNGRPLSAILAEQAG
TQPAEVLQEAEIAEGDAAEQAPTLEVVPEIANTPKTVAPEPKAPESKVAEYKAPDYKVPDSLTVSGYQQAQTGAAAKAPQ
ALPAAAAAADTISVSEYEASQAAKAAAAKAVMDYTRRQLRRR

Sequences:

>Translated_442_residues
MAAKVWSRAFRGSAIAASVSITAAVMAPSAHAEKYAAIVVDADSGEVLHARNQDDPRYPASLTKVMTLYLLFDAIDAGDV
SLKDKIKVSRNAAAQPPSNLRLRAGDTITVEDAIYALTTKSANDVAAAVAEFLGGSERKFAAMMTDKARDLGLSNTTFRN
ASGLPDTAQLSTARDLAILADALLDNHEPYYHYFQNQRFSWGGMTYKSHNNLIGSVDGVDGIKTGYTRASGFNLMTSAER
DGRRVIAIVLGGASAKSRDAHVTELVESAFVSIGQTPDLIDPNTKLMSFAALDTPLNPNAAAEPMLNGRPLSAILAEQAG
TQPAEVLQEAEIAEGDAAEQAPTLEVVPEIANTPKTVAPEPKAPESKVAEYKAPDYKVPDSLTVSGYQQAQTGAAAKAPQ
ALPAAAAAADTISVSEYEASQAAKAAAAKAVMDYTRRQLRRR
>Mature_441_residues
AAKVWSRAFRGSAIAASVSITAAVMAPSAHAEKYAAIVVDADSGEVLHARNQDDPRYPASLTKVMTLYLLFDAIDAGDVS
LKDKIKVSRNAAAQPPSNLRLRAGDTITVEDAIYALTTKSANDVAAAVAEFLGGSERKFAAMMTDKARDLGLSNTTFRNA
SGLPDTAQLSTARDLAILADALLDNHEPYYHYFQNQRFSWGGMTYKSHNNLIGSVDGVDGIKTGYTRASGFNLMTSAERD
GRRVIAIVLGGASAKSRDAHVTELVESAFVSIGQTPDLIDPNTKLMSFAALDTPLNPNAAAEPMLNGRPLSAILAEQAGT
QPAEVLQEAEIAEGDAAEQAPTLEVVPEIANTPKTVAPEPKAPESKVAEYKAPDYKVPDSLTVSGYQQAQTGAAAKAPQA
LPAAAAAADTISVSEYEASQAAKAAAAKAVMDYTRRQLRRR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=246, Percent_Identity=36.1788617886179, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787062, Length=253, Percent_Identity=35.9683794466403, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786851, Length=271, Percent_Identity=34.3173431734317, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI87082054, Length=227, Percent_Identity=28.1938325991189, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46697; Mature: 46566

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKVWSRAFRGSAIAASVSITAAVMAPSAHAEKYAAIVVDADSGEVLHARNQDDPRYPA
CCHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCH
SLTKVMTLYLLFDAIDAGDVSLKDKIKVSRNAAAQPPSNLRLRAGDTITVEDAIYALTTK
HHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCCCCCCEEECCCEEEECCEEEEEECC
SANDVAAAVAEFLGGSERKFAAMMTDKARDLGLSNTTFRNASGLPDTAQLSTARDLAILA
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DALLDNHEPYYHYFQNQRFSWGGMTYKSHNNLIGSVDGVDGIKTGYTRASGFNLMTSAER
HHHHCCCCCHHHHHCCCEEECCCEEEECCCCCEECCCCCCCCCCCCCCCCCCEEEECCCC
DGRRVIAIVLGGASAKSRDAHVTELVESAFVSIGQTPDLIDPNTKLMSFAALDTPLNPNA
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCCCCC
AAEPMLNGRPLSAILAEQAGTQPAEVLQEAEIAEGDAAEQAPTLEVVPEIANTPKTVAPE
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHCCCCHHCCHHHHCCCCCCCCC
PKAPESKVAEYKAPDYKVPDSLTVSGYQQAQTGAAAKAPQALPAAAAAADTISVSEYEAS
CCCCHHHHHHCCCCCCCCCCCEEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHHHH
QAAKAAAAKAVMDYTRRQLRRR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAKVWSRAFRGSAIAASVSITAAVMAPSAHAEKYAAIVVDADSGEVLHARNQDDPRYPA
CHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCH
SLTKVMTLYLLFDAIDAGDVSLKDKIKVSRNAAAQPPSNLRLRAGDTITVEDAIYALTTK
HHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCCCCCCEEECCCEEEECCEEEEEECC
SANDVAAAVAEFLGGSERKFAAMMTDKARDLGLSNTTFRNASGLPDTAQLSTARDLAILA
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DALLDNHEPYYHYFQNQRFSWGGMTYKSHNNLIGSVDGVDGIKTGYTRASGFNLMTSAER
HHHHCCCCCHHHHHCCCEEECCCEEEECCCCCEECCCCCCCCCCCCCCCCCCEEEECCCC
DGRRVIAIVLGGASAKSRDAHVTELVESAFVSIGQTPDLIDPNTKLMSFAALDTPLNPNA
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCCCCC
AAEPMLNGRPLSAILAEQAGTQPAEVLQEAEIAEGDAAEQAPTLEVVPEIANTPKTVAPE
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHCCCCHHCCHHHHCCCCCCCCC
PKAPESKVAEYKAPDYKVPDSLTVSGYQQAQTGAAAKAPQALPAAAAAADTISVSEYEAS
CCCCHHHHHHCCCCCCCCCCCEEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHHHH
QAAKAAAAKAVMDYTRRQLRRR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]