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The map label for this gene is pheA [H]

Identifier: 114799113

GI number: 114799113

Start: 660931

End: 661767

Strand: Direct

Name: pheA [H]

Synonym: HNE_0659

Alternate gene names: 114799113

Gene position: 660931-661767 (Clockwise)

Preceding gene: 114799232

Following gene: 114797703

Centisome position: 17.84

GC content: 60.93

Gene sequence:

>837_bases
ATGACCAGCAAAATAGCCTATCAGGGCGAACCCGGCGCGAACTCGCATATTGCCTGCGGCGAAGCGTTTCCGGGCTTTGA
GCCGATGGCATGCCGGACGTTCGAGGATTGCTTCATCGCCGTGGAGCGGGGCGAGGCCGAGCTTGCCATGATCCCGGTCG
AAAATACCATCGCCGGCCGGGTGGGCGACATTCACTATCTGCTGCCCACCACTCAGTTGCACATCACCGGCGAATACTAC
CTGCCGATCCGGTTTCAGCTGATGGCGCTGCCGGGCACCAGGCTAGAGGATGTCAAAAAGGCGCGTTCCCACATCATGGG
TCTTGGCCAGTGCCGTAACTTCCTGCGCAAGCATGCCATTGACCCGATCACTGCGGCGGACACAGCGGGCGCGGCAAGGG
AGGTCTCCGAACTGAACGACCCAAGCGTTGCGGCCATTGCCCCGCGCCTTGCCGCAGAAGTCTACGGTCTGGAAATCCTC
GCCGAGAATATCGAGGACGCCGCCCATAACACGACCCGCTTCGTCATCATGTCGCGCGAGCCCGCCGAGATCGATGCGGG
CGACGGCCCTGCCAAGACGGCGTTCATCTTTGAGGTCCGCAACATCCCCGCTGCGCTCTACAAGGGCCTTGGCGGATTTG
CCACCAACGGCGTCAACATGACCAAGCTGGAAAGCTATCTGGTGGGCGGCTCTTTCGAAGCGACGCAATTCTATGCCGAA
ATCGAAGGCCACCCTGATGAGCGCCCGGTCCAGCTTGCGCTTGAGGAGTTGGGCTTCTTCTCGCAGTCGCTGAAAATCCT
TGGCGTCTTTCCCAAAGCGATCAACGCGCGGCTGTAG

Upstream 100 bases:

>100_bases
CGGGTGCGCACAATATAAAGGGTGCCTCTCCAGGGCGCTTCCCCCCGTACTCGCGGCGATCAGACGCATGCCGGGGCCAT
TCAATACAGGGATTTTCGGA

Downstream 100 bases:

>100_bases
CGGCCTCCAGCCGGGCCATTGAACAGGCGTGGGCAATGGCTGGCTCGATATGTTCGACAAACAGCCGTGCCTTGCCGCGC
AGCTTGCCTGAGGTCCAGAC

Product: prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTSKIAYQGEPGANSHIACGEAFPGFEPMACRTFEDCFIAVERGEAELAMIPVENTIAGRVGDIHYLLPTTQLHITGEYY
LPIRFQLMALPGTRLEDVKKARSHIMGLGQCRNFLRKHAIDPITAADTAGAAREVSELNDPSVAAIAPRLAAEVYGLEIL
AENIEDAAHNTTRFVIMSREPAEIDAGDGPAKTAFIFEVRNIPAALYKGLGGFATNGVNMTKLESYLVGGSFEATQFYAE
IEGHPDERPVQLALEELGFFSQSLKILGVFPKAINARL

Sequences:

>Translated_278_residues
MTSKIAYQGEPGANSHIACGEAFPGFEPMACRTFEDCFIAVERGEAELAMIPVENTIAGRVGDIHYLLPTTQLHITGEYY
LPIRFQLMALPGTRLEDVKKARSHIMGLGQCRNFLRKHAIDPITAADTAGAAREVSELNDPSVAAIAPRLAAEVYGLEIL
AENIEDAAHNTTRFVIMSREPAEIDAGDGPAKTAFIFEVRNIPAALYKGLGGFATNGVNMTKLESYLVGGSFEATQFYAE
IEGHPDERPVQLALEELGFFSQSLKILGVFPKAINARL
>Mature_277_residues
TSKIAYQGEPGANSHIACGEAFPGFEPMACRTFEDCFIAVERGEAELAMIPVENTIAGRVGDIHYLLPTTQLHITGEYYL
PIRFQLMALPGTRLEDVKKARSHIMGLGQCRNFLRKHAIDPITAADTAGAAREVSELNDPSVAAIAPRLAAEVYGLEILA
ENIEDAAHNTTRFVIMSREPAEIDAGDGPAKTAFIFEVRNIPAALYKGLGGFATNGVNMTKLESYLVGGSFEATQFYAEI
EGHPDERPVQLALEELGFFSQSLKILGVFPKAINARL

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=276, Percent_Identity=32.9710144927536, Blast_Score=141, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 30186; Mature: 30055

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00857 PREPHENATE_DEHYDR_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKIAYQGEPGANSHIACGEAFPGFEPMACRTFEDCFIAVERGEAELAMIPVENTIAGR
CCCCEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHEEEEEECCCCEEEEEECCCCCCCC
VGDIHYLLPTTQLHITGEYYLPIRFQLMALPGTRLEDVKKARSHIMGLGQCRNFLRKHAI
CCCEEEEECCEEEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
DPITAADTAGAAREVSELNDPSVAAIAPRLAAEVYGLEILAENIEDAAHNTTRFVIMSRE
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
PAEIDAGDGPAKTAFIFEVRNIPAALYKGLGGFATNGVNMTKLESYLVGGSFEATQFYAE
CCCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHEEEEE
IEGHPDERPVQLALEELGFFSQSLKILGVFPKAINARL
ECCCCCCCHHHHHHHHHHHHHHCCEEEEECHHHHCCCC
>Mature Secondary Structure 
TSKIAYQGEPGANSHIACGEAFPGFEPMACRTFEDCFIAVERGEAELAMIPVENTIAGR
CCCEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHEEEEEECCCCEEEEEECCCCCCCC
VGDIHYLLPTTQLHITGEYYLPIRFQLMALPGTRLEDVKKARSHIMGLGQCRNFLRKHAI
CCCEEEEECCEEEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
DPITAADTAGAAREVSELNDPSVAAIAPRLAAEVYGLEILAENIEDAAHNTTRFVIMSRE
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
PAEIDAGDGPAKTAFIFEVRNIPAALYKGLGGFATNGVNMTKLESYLVGGSFEATQFYAE
CCCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHEEEEE
IEGHPDERPVQLALEELGFFSQSLKILGVFPKAINARL
ECCCCCCCHHHHHHHHHHHHHHCCEEEEECHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]