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The map label for this gene is yngF [H]

Identifier: 114798668

GI number: 114798668

Start: 1114176

End: 1114958

Strand: Reverse

Name: yngF [H]

Synonym: HNE_1107

Alternate gene names: 114798668

Gene position: 1114958-1114176 (Counterclockwise)

Preceding gene: 114799768

Following gene: 114799441

Centisome position: 30.09

GC content: 65.64

Gene sequence:

>783_bases
ATGACCCTGCCCATCCGCCTCGATATTGCCGCCCCGCTCGCCGAAATCGTGCTCAACAAGCCCGAGCGCCGCAACGCGCT
CTCGGTCGACATGTGGGCCGCGATCCCCGGCCTCGTGGCAGAGGCAAATGCGAATCCGGACGTAAAGCTGATCCTGATCC
ATGGTGGAGACGCCGGCGCTTTCGCCGCGGGCGCGGACATCTCCGAATTTGAGACGATCTACGCCACCGAGGACGCCGCC
AAAGCCTCCGGCCAGCGCATCGCCCAGGCCCTGGACGCCATCGAGAACAGCGAAAAGCCCGTCATCGCTGCGATCGAAGG
CGCCTGCGTGGGCGGCGGTGTCAGCCTCGCCATGGCGGCAGACCTGCGCGTGGCTGGCGAGGGCGCAAAATTCGGCGTCA
CGCCCGGCAAGCTCGGCCTCGTCTATCCGGCCGGCGACACACGCCGCCTCCTCGCCGCTGTCGGCCCCGGCGCCACCAAG
GACATCCTCTTTACAGGGCGCATCTTCACCGCCGGCGAAGCCAAATCCCTTGGCCTCATAGACCGCCTCGTGGAAAAGGG
CACCGCCCTCGAAGCCGCCCGCGTCTGGGCCGGCGAGATTGCCGCCATCTCGCAATGGTCGGTCCGCGCCACCAAGCGTA
TGATCCGTGGACTGCAAACCGGCTGGACGGATGAAACTCCGGAGGCTCAGAGCCTCTTCCTCAACGGCTTCGCGAATGAG
GATTTCAAGGAAGGCTACCGCGCCTTCCTCGATAAACGCCCGGCCAAATTCACCTATCGCTGA

Upstream 100 bases:

>100_bases
GTATGGGGCCTGCCGGTTTCCAGCGCTTCTTGCGCCGCAAGGCGCTGATCGCTAACACCGGCGCGCCAGCCCCGCTTTCC
GCCTTTGCCGAGGACGCCCA

Downstream 100 bases:

>100_bases
AGCGCCAGCGGCACACGCGGCGCGCCAAACAACGCTTCCAATGCCTGCGCCATATCCAGCACGCGCGCGTCCGATCCCTT
CGGACCGATCACCTGGACCG

Product: enoyl-CoA hydratase/isomerase family protein

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTLPIRLDIAAPLAEIVLNKPERRNALSVDMWAAIPGLVAEANANPDVKLILIHGGDAGAFAAGADISEFETIYATEDAA
KASGQRIAQALDAIENSEKPVIAAIEGACVGGGVSLAMAADLRVAGEGAKFGVTPGKLGLVYPAGDTRRLLAAVGPGATK
DILFTGRIFTAGEAKSLGLIDRLVEKGTALEAARVWAGEIAAISQWSVRATKRMIRGLQTGWTDETPEAQSLFLNGFANE
DFKEGYRAFLDKRPAKFTYR

Sequences:

>Translated_260_residues
MTLPIRLDIAAPLAEIVLNKPERRNALSVDMWAAIPGLVAEANANPDVKLILIHGGDAGAFAAGADISEFETIYATEDAA
KASGQRIAQALDAIENSEKPVIAAIEGACVGGGVSLAMAADLRVAGEGAKFGVTPGKLGLVYPAGDTRRLLAAVGPGATK
DILFTGRIFTAGEAKSLGLIDRLVEKGTALEAARVWAGEIAAISQWSVRATKRMIRGLQTGWTDETPEAQSLFLNGFANE
DFKEGYRAFLDKRPAKFTYR
>Mature_259_residues
TLPIRLDIAAPLAEIVLNKPERRNALSVDMWAAIPGLVAEANANPDVKLILIHGGDAGAFAAGADISEFETIYATEDAAK
ASGQRIAQALDAIENSEKPVIAAIEGACVGGGVSLAMAADLRVAGEGAKFGVTPGKLGLVYPAGDTRRLLAAVGPGATKD
ILFTGRIFTAGEAKSLGLIDRLVEKGTALEAARVWAGEIAAISQWSVRATKRMIRGLQTGWTDETPEAQSLFLNGFANED
FKEGYRAFLDKRPAKFTYR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=33.7448559670782, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI20127408, Length=186, Percent_Identity=32.7956989247312, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI70995211, Length=209, Percent_Identity=31.1004784688995, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4502327, Length=245, Percent_Identity=30.6122448979592, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI68989263, Length=206, Percent_Identity=33.495145631068, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=29.2, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=206, Percent_Identity=24.2718446601942, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI45643119, Length=206, Percent_Identity=24.2718446601942, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI260275230, Length=206, Percent_Identity=24.2718446601942, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=32.8, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082183, Length=250, Percent_Identity=26.4, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=29.1666666666667, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=31.1224489795918, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=25.7028112449799, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790281, Length=183, Percent_Identity=27.3224043715847, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787660, Length=248, Percent_Identity=27.8225806451613, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=257, Percent_Identity=28.0155642023346, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=33.739837398374, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=180, Percent_Identity=30.5555555555556, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=263, Percent_Identity=30.7984790874525, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=180, Percent_Identity=30, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=229, Percent_Identity=29.2576419213974, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=192, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=212, Percent_Identity=29.7169811320755, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=212, Percent_Identity=29.7169811320755, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=249, Percent_Identity=28.1124497991968, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=28.0632411067194, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI19920382, Length=251, Percent_Identity=31.8725099601594, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653139, Length=247, Percent_Identity=31.1740890688259, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=32.03125, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=32.03125, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24650670, Length=190, Percent_Identity=32.6315789473684, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357171, Length=236, Percent_Identity=27.1186440677966, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19921000, Length=179, Percent_Identity=32.9608938547486, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24583077, Length=179, Percent_Identity=32.9608938547486, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583079, Length=179, Percent_Identity=32.9608938547486, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583165, Length=205, Percent_Identity=27.3170731707317, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 27411; Mature: 27279

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPIRLDIAAPLAEIVLNKPERRNALSVDMWAAIPGLVAEANANPDVKLILIHGGDAGA
CCCEEEEEHHHHHHHHHCCCCCCCCCEEEEHHHHCCCCEEECCCCCCEEEEEEECCCCCC
FAAGADISEFETIYATEDAAKASGQRIAQALDAIENSEKPVIAAIEGACVGGGVSLAMAA
EECCCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEEEEE
DLRVAGEGAKFGVTPGKLGLVYPAGDTRRLLAAVGPGATKDILFTGRIFTAGEAKSLGLI
CEEEECCCCEECCCCCCEEEEECCCCHHHHHHHCCCCCCCEEEEECEEEECCCCCHHHHH
DRLVEKGTALEAARVWAGEIAAISQWSVRATKRMIRGLQTGWTDETPEAQSLFLNGFANE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCCC
DFKEGYRAFLDKRPAKFTYR
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TLPIRLDIAAPLAEIVLNKPERRNALSVDMWAAIPGLVAEANANPDVKLILIHGGDAGA
CCEEEEEHHHHHHHHHCCCCCCCCCEEEEHHHHCCCCEEECCCCCCEEEEEEECCCCCC
FAAGADISEFETIYATEDAAKASGQRIAQALDAIENSEKPVIAAIEGACVGGGVSLAMAA
EECCCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEEEEE
DLRVAGEGAKFGVTPGKLGLVYPAGDTRRLLAAVGPGATKDILFTGRIFTAGEAKSLGLI
CEEEECCCCEECCCCCCEEEEECCCCHHHHHHHCCCCCCCEEEEECEEEECCCCCHHHHH
DRLVEKGTALEAARVWAGEIAAISQWSVRATKRMIRGLQTGWTDETPEAQSLFLNGFANE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCCC
DFKEGYRAFLDKRPAKFTYR
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]