Click here to switch to the map view.

The map label for this gene is tolB [H]

Identifier: 114798605

GI number: 114798605

Start: 135616

End: 136971

Strand: Direct

Name: tolB [H]

Synonym: HNE_0154

Alternate gene names: 114798605

Gene position: 135616-136971 (Clockwise)

Preceding gene: 114798960

Following gene: 114797840

Centisome position: 3.66

GC content: 63.05

Gene sequence:

>1356_bases
ATGCTGAAACGCCTGATGCCGTTCCTGGCAGTCATCATGCTGGCCGTTGGGCTGGTGGCGCCTGCCAGCGCCCAGCTGCA
GGTCCGTGTGGATGAAGGGAACTTCAAACCCACCCCCATCGCCATTCCGGACTTCCAGGTTACGGGCGCCAATACCGAGA
TCGCCCGCCAGATTACAGAAGTGGTGCGCGCCGACCTCGCCTCCACCGGCCTGTTCGACATGCAGAACCCGGCCTCCTTC
ATCCAGCGCGACCTCTCGATCAATGCCGAGCCGCGCTTCGCAGACTGGAAGATCATCCGCACGGATGCGCTTGTCGTGGG
CGCGTTTGAAGAGCTGCCCGATGGCAAGCTCGCCGTGTCGTTCCGCCTCTGGGACGTTTATGGCGGGGAGTTGATGCGCA
TCAATGGTGAGCCCGGCCGCCGCCTGACCACCACGCCGGAGAACTGGCGCCGCATTGCCCACAAGATTGCGGACTCGATC
TATACCCGCCTGACCGGTGAAGACCCGTATTTCGACACGCGGATCGTCTACATCGCCGAGACCGGCCCGAAGACCGAGCG
CGTCAAGCGTCTGGCCATCATGGACAGCGACGGCGCCAACCAGCAGTATCTTACCTCCGGCCGCTCGACCGTGCTGACGC
CCCGCTTCAGCCCGTCCTCGCAGGAAATCACCTACATGTCGTTTGAGGGGGGGCGCCCGCGCGTCTACCTCTTCAATATC
GAAACAGGCCGCCAGGAACTGCTCGGGAATTTCCCCGGCATGACGTTTGCCCCGCGCTTTGGCACGGATGGTCAGAGCGT
GCTGCTGTCGCAGGCCCAGCGCGGCAACACCGATGTCTACATCATGAACCTGCGGACGCGCGAAAGCCGCCGCCTGACGG
ACCATCCGGCGATCGACACCTCGCCTTCCATGTCTCCGGATGGCAAATCGGTCGTGTTCAACTCCGACCGGGGTGGCACG
CCGCAGCTCTATATCATGAACACAGACGGCTCACCGCGCGCCTGCGCGTCGGGCGGCCGGGATACGGCCTGCCGGATCAC
CTTCGGGCGCGGTCGGTATTCCACTCCCGTCTGGTCCCCGCGCGGTGACCTGATTGCTTTCACGAAGCAGGAAGGCGGAA
AATTCTATATCGGTGTCATCGGCACGGATGGCAAAGGCGAGCGCCTGTTGTCGGAATCCTATCTGGATGAAGGTCCGGAT
TGGTCGCCAAATGGCCGCGTGATCGTTTTCTTCCGTGAGAATCGCCCTGGCGCGGGTGCACAGCTCTGGTCGGTTGACCT
TTCCGGCCGGAATGAGCGCCGTGTGATGACCCAAACGGACGCTTCGGACCCTGCCTGGTCGCCGGTTCTGGAATAG

Upstream 100 bases:

>100_bases
GCGGAAGATTATGATCTTTGGAAAGATATCAACGTGACGCTTGGCCCGAAACAGGGTTGAGACATGCGCGTCGCAAGAAT
TCGATGAGGAGTGCGAACTG

Downstream 100 bases:

>100_bases
TCTTTATTGAACAAGGCGCCTGGCCGGGTGCTTAAAGCCCGGCCAGCACGTCAGAATATGGCCACTTCGTGGCAAAAACA
GCACACCGGGAGAAATATTC

Product: tolB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MLKRLMPFLAVIMLAVGLVAPASAQLQVRVDEGNFKPTPIAIPDFQVTGANTEIARQITEVVRADLASTGLFDMQNPASF
IQRDLSINAEPRFADWKIIRTDALVVGAFEELPDGKLAVSFRLWDVYGGELMRINGEPGRRLTTTPENWRRIAHKIADSI
YTRLTGEDPYFDTRIVYIAETGPKTERVKRLAIMDSDGANQQYLTSGRSTVLTPRFSPSSQEITYMSFEGGRPRVYLFNI
ETGRQELLGNFPGMTFAPRFGTDGQSVLLSQAQRGNTDVYIMNLRTRESRRLTDHPAIDTSPSMSPDGKSVVFNSDRGGT
PQLYIMNTDGSPRACASGGRDTACRITFGRGRYSTPVWSPRGDLIAFTKQEGGKFYIGVIGTDGKGERLLSESYLDEGPD
WSPNGRVIVFFRENRPGAGAQLWSVDLSGRNERRVMTQTDASDPAWSPVLE

Sequences:

>Translated_451_residues
MLKRLMPFLAVIMLAVGLVAPASAQLQVRVDEGNFKPTPIAIPDFQVTGANTEIARQITEVVRADLASTGLFDMQNPASF
IQRDLSINAEPRFADWKIIRTDALVVGAFEELPDGKLAVSFRLWDVYGGELMRINGEPGRRLTTTPENWRRIAHKIADSI
YTRLTGEDPYFDTRIVYIAETGPKTERVKRLAIMDSDGANQQYLTSGRSTVLTPRFSPSSQEITYMSFEGGRPRVYLFNI
ETGRQELLGNFPGMTFAPRFGTDGQSVLLSQAQRGNTDVYIMNLRTRESRRLTDHPAIDTSPSMSPDGKSVVFNSDRGGT
PQLYIMNTDGSPRACASGGRDTACRITFGRGRYSTPVWSPRGDLIAFTKQEGGKFYIGVIGTDGKGERLLSESYLDEGPD
WSPNGRVIVFFRENRPGAGAQLWSVDLSGRNERRVMTQTDASDPAWSPVLE
>Mature_451_residues
MLKRLMPFLAVIMLAVGLVAPASAQLQVRVDEGNFKPTPIAIPDFQVTGANTEIARQITEVVRADLASTGLFDMQNPASF
IQRDLSINAEPRFADWKIIRTDALVVGAFEELPDGKLAVSFRLWDVYGGELMRINGEPGRRLTTTPENWRRIAHKIADSI
YTRLTGEDPYFDTRIVYIAETGPKTERVKRLAIMDSDGANQQYLTSGRSTVLTPRFSPSSQEITYMSFEGGRPRVYLFNI
ETGRQELLGNFPGMTFAPRFGTDGQSVLLSQAQRGNTDVYIMNLRTRESRRLTDHPAIDTSPSMSPDGKSVVFNSDRGGT
PQLYIMNTDGSPRACASGGRDTACRITFGRGRYSTPVWSPRGDLIAFTKQEGGKFYIGVIGTDGKGERLLSESYLDEGPD
WSPNGRVIVFFRENRPGAGAQLWSVDLSGRNERRVMTQTDASDPAWSPVLE

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=433, Percent_Identity=28.6374133949192, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 50013; Mature: 50013

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRLMPFLAVIMLAVGLVAPASAQLQVRVDEGNFKPTPIAIPDFQVTGANTEIARQITE
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEECCCEEEECCCHHHHHHHHH
VVRADLASTGLFDMQNPASFIQRDLSINAEPRFADWKIIRTDALVVGAFEELPDGKLAVS
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEECEEEEECHHHCCCCEEEEE
FRLWDVYGGELMRINGEPGRRLTTTPENWRRIAHKIADSIYTRLTGEDPYFDTRIVYIAE
EEEEEECCCEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
TGPKTERVKRLAIMDSDGANQQYLTSGRSTVLTPRFSPSSQEITYMSFEGGRPRVYLFNI
CCCCHHHEEEEEEECCCCCCCHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEEEE
ETGRQELLGNFPGMTFAPRFGTDGQSVLLSQAQRGNTDVYIMNLRTRESRRLTDHPAIDT
CCCHHHHHHCCCCCEECCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCC
SPSMSPDGKSVVFNSDRGGTPQLYIMNTDGSPRACASGGRDTACRITFGRGRYSTPVWSP
CCCCCCCCCEEEEECCCCCCCEEEEEECCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCC
RGDLIAFTKQEGGKFYIGVIGTDGKGERLLSESYLDEGPDWSPNGRVIVFFRENRPGAGA
CCCEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCC
QLWSVDLSGRNERRVMTQTDASDPAWSPVLE
EEEEEECCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLKRLMPFLAVIMLAVGLVAPASAQLQVRVDEGNFKPTPIAIPDFQVTGANTEIARQITE
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEECCCEEEECCCHHHHHHHHH
VVRADLASTGLFDMQNPASFIQRDLSINAEPRFADWKIIRTDALVVGAFEELPDGKLAVS
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEECEEEEECHHHCCCCEEEEE
FRLWDVYGGELMRINGEPGRRLTTTPENWRRIAHKIADSIYTRLTGEDPYFDTRIVYIAE
EEEEEECCCEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
TGPKTERVKRLAIMDSDGANQQYLTSGRSTVLTPRFSPSSQEITYMSFEGGRPRVYLFNI
CCCCHHHEEEEEEECCCCCCCHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEEEE
ETGRQELLGNFPGMTFAPRFGTDGQSVLLSQAQRGNTDVYIMNLRTRESRRLTDHPAIDT
CCCHHHHHHCCCCCEECCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCC
SPSMSPDGKSVVFNSDRGGTPQLYIMNTDGSPRACASGGRDTACRITFGRGRYSTPVWSP
CCCCCCCCCEEEEECCCCCCCEEEEEECCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCC
RGDLIAFTKQEGGKFYIGVIGTDGKGERLLSESYLDEGPDWSPNGRVIVFFRENRPGAGA
CCCEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCC
QLWSVDLSGRNERRVMTQTDASDPAWSPVLE
EEEEEECCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]