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The map label for this gene is rph [H]

Identifier: 114798422

GI number: 114798422

Start: 3468445

End: 3469170

Strand: Reverse

Name: rph [H]

Synonym: HNE_3315

Alternate gene names: 114798422

Gene position: 3469170-3468445 (Counterclockwise)

Preceding gene: 114797965

Following gene: 114800076

Centisome position: 93.63

GC content: 65.29

Gene sequence:

>726_bases
ATGCCAAATCTTGTTCGCCCCACGGGCCGCGCTTTTGACGAGCTGCGCCCGATTTCGCTCGAAACCAGCGTGACCCGCTA
TGCTGAAGGCTCGTGCCTGGCCAAGTTTGGCCATACGCATGTGCTGTGCACGGCGAGCTGGGAAAAGGGCGTGCCCGGCT
GGCTGAAGGGCAAGGGCGAGGGCTGGGTGACGGGCGAATATGGCATGCTGCCGCGCGCGACGCACACGCGCGGGCGCCGG
GAAGCGGCGGCAGGCAAACAGTCTGGCCGGACGCAGGAAATCCAGCGCCTGATCGGGCGCTCGCTGCGCTCGGTCGTCGA
CTTGAAAGCGCTGGGCGAGAACACGATCACCATCGACTGTGATGTGATCCAGGCCGATGGCGGCACGCGCACCGCCTCGA
TCACCGGCGGCTTTGTCGCTCTCGCCATGGCCTGCCGCTATCTGCAGACCGAAGGCGTGATCAAGGCGAGCCCGATCCAG
AAACAGGCCGCCGCGATCTCCGTGGGCCTGTTCCAGAACCATCCGGTGCTGGACGTGGACTATCCTGAAGATTCCGAAGG
CGAAGCCGACATGAATGTCGTGATGAGCTCGGATGGCGGGTTCATCGAGGTGCAGGGCACGGGCGAGTCGCGGCCGATGA
GCCGGACAGAGCTCGACGAGATGATGCGACTGGCCGAGAAGGGCTGCAACGAGCTGTTTGCGGCGCAGCGGGCGGCGATC
GGCTGA

Upstream 100 bases:

>100_bases
CACTGGCCATATTCGGTACTTTCCTTTCGCGCGCAGCCTTGGTGTAGACAGGCGCCGCAAAACCCGCAAATCAGGCCTTC
AGCTAGAAGGATTCCCCCGG

Downstream 100 bases:

>100_bases
TCAGTAGAGTGCGGTGAGCTGGTTAGCCGTCAGGGCAAACAGCGTTGCGATCGTCAGGTTACAGAGGGTTCCCAGCAGGT
AGTAGGACGGGTTCTGCTGG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MPNLVRPTGRAFDELRPISLETSVTRYAEGSCLAKFGHTHVLCTASWEKGVPGWLKGKGEGWVTGEYGMLPRATHTRGRR
EAAAGKQSGRTQEIQRLIGRSLRSVVDLKALGENTITIDCDVIQADGGTRTASITGGFVALAMACRYLQTEGVIKASPIQ
KQAAAISVGLFQNHPVLDVDYPEDSEGEADMNVVMSSDGGFIEVQGTGESRPMSRTELDEMMRLAEKGCNELFAAQRAAI
G

Sequences:

>Translated_241_residues
MPNLVRPTGRAFDELRPISLETSVTRYAEGSCLAKFGHTHVLCTASWEKGVPGWLKGKGEGWVTGEYGMLPRATHTRGRR
EAAAGKQSGRTQEIQRLIGRSLRSVVDLKALGENTITIDCDVIQADGGTRTASITGGFVALAMACRYLQTEGVIKASPIQ
KQAAAISVGLFQNHPVLDVDYPEDSEGEADMNVVMSSDGGFIEVQGTGESRPMSRTELDEMMRLAEKGCNELFAAQRAAI
G
>Mature_240_residues
PNLVRPTGRAFDELRPISLETSVTRYAEGSCLAKFGHTHVLCTASWEKGVPGWLKGKGEGWVTGEYGMLPRATHTRGRRE
AAAGKQSGRTQEIQRLIGRSLRSVVDLKALGENTITIDCDVIQADGGTRTASITGGFVALAMACRYLQTEGVIKASPIQK
QAAAISVGLFQNHPVLDVDYPEDSEGEADMNVVMSSDGGFIEVQGTGESRPMSRTELDEMMRLAEKGCNELFAAQRAAIG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=60.4761904761905, Blast_Score=263, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI71981632, Length=192, Percent_Identity=27.6041666666667, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25946; Mature: 25815

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNLVRPTGRAFDELRPISLETSVTRYAEGSCLAKFGHTHVLCTASWEKGVPGWLKGKGE
CCCCCCCCCHHHHHCCCCEECHHHHHHCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCC
GWVTGEYGMLPRATHTRGRREAAAGKQSGRTQEIQRLIGRSLRSVVDLKALGENTITIDC
EEEECCCCCCCCCHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
DVIQADGGTRTASITGGFVALAMACRYLQTEGVIKASPIQKQAAAISVGLFQNHPVLDVD
EEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCEECCCCHHHHHEEEEEEECCCCEEECC
YPEDSEGEADMNVVMSSDGGFIEVQGTGESRPMSRTELDEMMRLAEKGCNELFAAQRAAI
CCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure 
PNLVRPTGRAFDELRPISLETSVTRYAEGSCLAKFGHTHVLCTASWEKGVPGWLKGKGE
CCCCCCCCHHHHHCCCCEECHHHHHHCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCC
GWVTGEYGMLPRATHTRGRREAAAGKQSGRTQEIQRLIGRSLRSVVDLKALGENTITIDC
EEEECCCCCCCCCHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
DVIQADGGTRTASITGGFVALAMACRYLQTEGVIKASPIQKQAAAISVGLFQNHPVLDVD
EEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCEECCCCHHHHHEEEEEEECCCCEEECC
YPEDSEGEADMNVVMSSDGGFIEVQGTGESRPMSRTELDEMMRLAEKGCNELFAAQRAAI
CCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606155 [H]