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The map label for this gene is lip2 [H]

Identifier: 114798413

GI number: 114798413

Start: 3134275

End: 3135315

Strand: Reverse

Name: lip2 [H]

Synonym: HNE_2976

Alternate gene names: 114798413

Gene position: 3135315-3134275 (Counterclockwise)

Preceding gene: 114797609

Following gene: 114799638

Centisome position: 84.62

GC content: 64.46

Gene sequence:

>1041_bases
ATGAGCCTTCAACGCATGATCGCAAAGGCGATTGCCAGCCTGCCCGGCGGCATGATCGTCAAGATGTCGGGCGGCGAGCC
CAAGACCATCGAAGGCCGCACGCTGGAGCCACAACTCCAGCTTGTCGCCTGGAACGGCCGCAATGCGCCGCCGATGTCCA
CCCTTCCGGCAGAGGCCGTACAGCAGGGCGTAAAGGCCCAGCTCGCCCTCTTTCAGGACAAGCTGCCCGGCGGCGTTGGC
GTCGAGGAGTTCACCATTCCCGGCCCGGACGCAAACCAGATCCCCGTGCGCCTCTACATGCCCGCCTCCCAGGATCCCCG
CCATCCCCTCATCGTCTACTTCCACATGGGCGGCGGCGTTATCGGCGATATGGACACCTGCCACGCTTTCTGCGGCATGC
TCGCCCAGGGCGCCCAGGCGCCCGTTCTCTCGGTCGATTACCGCCTTGCCCCGCAGCACATTTATCCCGCCGGGCTGAAC
GATTGTTTTGCTGCCTATAAATGGGGCGTGGAAAACGCCGCCAAATACGGCGCCCCCGCCGGCAAGGCGTCGGTGGGCGG
CGACAGCATGGGCGGAAACTTCTCCGCCATCATCAGCCAGCGGATGAAGCGGGAAGGCGGCCCGCTGCCGGCGCTCCAGC
TGCTCATCTACCCCGCCATCGACATCTCGAAGCACTATCCCTCCAAGACCGCGTTCGCCAACACCTTCTCCCTCAGCCAG
GACACGATGGACTGGTTCATGCAGCAATACCTGCCTGAAGGCTTTGACTATACCGACCTCAACGTCTCGCCGGGCCAGGC
GGCAGACCTTGCCGGACTGCCCCCCGCCGTTGTGGTCACCGCCGGGCATGACCCGCTGGTGGACGAGGGCGACGAATATG
CCGAACGCCTGAAAGCGGCCGGTGTGACGGTCAAGCACAAGCGCTATGACAGCCTTGCCCACGCCTTCACCGCCTTCACC
TTCCTCTCGCCGGGATCGCGCACGGCCTGCACCGAAATCGCCCGTATGGTCCACGATATGTATGCGGCCAGGAAGGCATG
A

Upstream 100 bases:

>100_bases
ACGCGCGCGTATAATGCCACCGCCGCCGGGGAACCCCTTGGCGGCGGGCGAAAATGGCGTCAGGTTCTCCCTCAAGAACA
GTTGAGAGGGAGGGGTTTCC

Downstream 100 bases:

>100_bases
AGTCTCTCGTCGTAAGTGAGCTTGCTCCGGACTTTGCCGGAACGAGCCTGAAAGATCTTCCCCTGCCGGAGCCAAAGGCC
GGGGAGGTCCGCGTCGCCAT

Product: alpha/beta hydrolase fold family protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSLQRMIAKAIASLPGGMIVKMSGGEPKTIEGRTLEPQLQLVAWNGRNAPPMSTLPAEAVQQGVKAQLALFQDKLPGGVG
VEEFTIPGPDANQIPVRLYMPASQDPRHPLIVYFHMGGGVIGDMDTCHAFCGMLAQGAQAPVLSVDYRLAPQHIYPAGLN
DCFAAYKWGVENAAKYGAPAGKASVGGDSMGGNFSAIISQRMKREGGPLPALQLLIYPAIDISKHYPSKTAFANTFSLSQ
DTMDWFMQQYLPEGFDYTDLNVSPGQAADLAGLPPAVVVTAGHDPLVDEGDEYAERLKAAGVTVKHKRYDSLAHAFTAFT
FLSPGSRTACTEIARMVHDMYAARKA

Sequences:

>Translated_346_residues
MSLQRMIAKAIASLPGGMIVKMSGGEPKTIEGRTLEPQLQLVAWNGRNAPPMSTLPAEAVQQGVKAQLALFQDKLPGGVG
VEEFTIPGPDANQIPVRLYMPASQDPRHPLIVYFHMGGGVIGDMDTCHAFCGMLAQGAQAPVLSVDYRLAPQHIYPAGLN
DCFAAYKWGVENAAKYGAPAGKASVGGDSMGGNFSAIISQRMKREGGPLPALQLLIYPAIDISKHYPSKTAFANTFSLSQ
DTMDWFMQQYLPEGFDYTDLNVSPGQAADLAGLPPAVVVTAGHDPLVDEGDEYAERLKAAGVTVKHKRYDSLAHAFTAFT
FLSPGSRTACTEIARMVHDMYAARKA
>Mature_345_residues
SLQRMIAKAIASLPGGMIVKMSGGEPKTIEGRTLEPQLQLVAWNGRNAPPMSTLPAEAVQQGVKAQLALFQDKLPGGVGV
EEFTIPGPDANQIPVRLYMPASQDPRHPLIVYFHMGGGVIGDMDTCHAFCGMLAQGAQAPVLSVDYRLAPQHIYPAGLND
CFAAYKWGVENAAKYGAPAGKASVGGDSMGGNFSAIISQRMKREGGPLPALQLLIYPAIDISKHYPSKTAFANTFSLSQD
TMDWFMQQYLPEGFDYTDLNVSPGQAADLAGLPPAVVVTAGHDPLVDEGDEYAERLKAAGVTVKHKRYDSLAHAFTAFTF
LSPGSRTACTEIARMVHDMYAARKA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=329, Percent_Identity=28.2674772036474, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI68299767, Length=306, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157041239, Length=271, Percent_Identity=27.6752767527675, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI61966717, Length=299, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI68051721, Length=330, Percent_Identity=24.2424242424242, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI21328446, Length=152, Percent_Identity=32.8947368421053, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI226423947, Length=338, Percent_Identity=23.6686390532544, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786682, Length=231, Percent_Identity=23.3766233766234, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17567059, Length=276, Percent_Identity=28.9855072463768, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17540028, Length=138, Percent_Identity=31.1594202898551, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71996133, Length=171, Percent_Identity=28.6549707602339, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=135, Percent_Identity=32.5925925925926, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 37056; Mature: 36925

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQRMIAKAIASLPGGMIVKMSGGEPKTIEGRTLEPQLQLVAWNGRNAPPMSTLPAEAV
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCEEEEEEECCCCCCCCCCCCHHHH
QQGVKAQLALFQDKLPGGVGVEEFTIPGPDANQIPVRLYMPASQDPRHPLIVYFHMGGGV
HHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCC
IGDMDTCHAFCGMLAQGAQAPVLSVDYRLAPQHIYPAGLNDCFAAYKWGVENAAKYGAPA
CCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCHHHHHHHCCCC
GKASVGGDSMGGNFSAIISQRMKREGGPLPALQLLIYPAIDISKHYPSKTAFANTFSLSQ
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCCCHHHHHECCCCH
DTMDWFMQQYLPEGFDYTDLNVSPGQAADLAGLPPAVVVTAGHDPLVDEGDEYAERLKAA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHC
GVTVKHKRYDSLAHAFTAFTFLSPGSRTACTEIARMVHDMYAARKA
CCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLQRMIAKAIASLPGGMIVKMSGGEPKTIEGRTLEPQLQLVAWNGRNAPPMSTLPAEAV
CHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCEEEEEEECCCCCCCCCCCCHHHH
QQGVKAQLALFQDKLPGGVGVEEFTIPGPDANQIPVRLYMPASQDPRHPLIVYFHMGGGV
HHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCC
IGDMDTCHAFCGMLAQGAQAPVLSVDYRLAPQHIYPAGLNDCFAAYKWGVENAAKYGAPA
CCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCHHHHHHHCCCC
GKASVGGDSMGGNFSAIISQRMKREGGPLPALQLLIYPAIDISKHYPSKTAFANTFSLSQ
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHCCCCCCHHHHHECCCCH
DTMDWFMQQYLPEGFDYTDLNVSPGQAADLAGLPPAVVVTAGHDPLVDEGDEYAERLKAA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHC
GVTVKHKRYDSLAHAFTAFTFLSPGSRTACTEIARMVHDMYAARKA
CCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]