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The map label for this gene is parE [H]

Identifier: 114798323

GI number: 114798323

Start: 988841

End: 990838

Strand: Reverse

Name: parE [H]

Synonym: HNE_0976

Alternate gene names: 114798323

Gene position: 990838-988841 (Counterclockwise)

Preceding gene: 114799557

Following gene: 114799895

Centisome position: 26.74

GC content: 63.46

Gene sequence:

>1998_bases
ATGTCCTCAGCGCGGCAGATGAAGCAAGACGACCTGCTGGCGGGCACAGCGGGCTATTCCGCCAAGGACATCGAGGTCCT
CGAAGGCCTTGAGCCCGTCCGCAAGCGCCCCGGCATGTATATCGGCGGCACCGATGAGCGCGCCTGGCACCACCTCTTCG
CTGAAGTCCTCGACAACGCCATGGACGAAGCCGTTGCCGGCTATGCCAACCGCATCGACATCAAGCTCGACGCTGACGGC
TTCCTCACCGTGGTCGACAATGGCCGCGGCATCCCGATCGACCCGCACCCGAAATTCCCCAAGAAATCAGCGCTCGAAGT
CATCATGACGACGCTCCATTCGGGCGGCAAATTCTCCGACAAAGCCTACTCCACCGCCGGAGGCCTCCACGGCGTCGGCA
TCTCGGTCGTCAACGCCCTGTCTGAACTCGTCGAAGTCGAAGTCGCCCGCGACCGCACGCTCTACCGCCAGGATTTCTCG
CGCGGCCTGCCGCTCGGCAAGCTCCAGAAGGTCGGCGCTGCGCCCAACCGGCGCGGCACCTCCGTGCGCTTCAAGCCCGA
CTTCCAGATCTTCGGCGACAAGCTCCGCTGGCGCCCCCAGCGCCTCTTCCAGATGGCGCGCTCGAAGGCTTACCTCTTCC
GCGGCGTCGAAGTGCGCTGGTCCTGCGATCCCGAGCTTCTTCCCGAGGACTCCAAAGTCCCCGCCGAAGCCGTCCTCTCC
TATCCCAATGGCCTGGCCGACCAGCTCACCGAAGTCTTCGGCGACAAGTCCACCATCACCGAGACCCCCTTCACCGGCCT
CGTTGACATGGGCGCCGAGGGTAAGGTCGAATGGGCCATCGCCTGGACCCAGGCCGGCTTTGGCGAGGCAGACGGCTTCG
CCCGCTCCTATTGTAACACCATCCCGACGCCCGAAGGCGGCACCCACGAAGCCGGCTTCCGCTCGGCCATCACCAAGGGC
ATCCGCAATTTCGGAGACCTGACCGGCAACAAGAAAGCCGCCGAAGTCACCGCCGAGGATGTCATGGGCCATTCCGGCCT
CCTCCTCTCGGTCTTCATCCGTGGCCCTGAATTCGTCGGCCAGACCAAGGACAAGCTCTCCTCCACCCACGCCTTCCGCT
TGGTGGAAAACGCGGTACGGGACCACTTCGACCATTGGCTCGCCGGCTCCCCCAAGGAAGCAAACAAGCTGCTCGGCTGG
GCCATCGACCGCGCCGATGAACGCGCCAAGCGCCGCAAGGCCAAGGAAATCAGCCGAAAGTCTGCCACCAAGAAACTCCG
CCTCCCCGGCAAACTCGCCGATTGCTCGGCTAAAGGCCCGGAAGGCACCGAACTCTTCCTCGTCGAAGGCGACTCGGCCG
GCGGCTCCGCCAAACAGGCCCGCAACCGCGAGACGCAGGCGATCCTCCCCCTGCGCGGCAAGATCCTCAACGTCGAAAGC
GCTTCAGACGACAAGCTGATGGGCAACCAGGAACTGGCCGACCTCTCCCTCGCCCTCGGCACAGAACTCGGTCGCAAGTT
CAACATCGACGATCTGCGCTATGAGCGCATCATCATCATGACCGATGCGGACGTCGACGGGGCCCACATCGCCGCCCTGC
TCATCACCTTCTTCTACCGGCTCACCCCCGGCCTGATCGAAAGCGGGCGGCTCTATCTCGCCCTGCCGCCCCTGTTCAAA
CTCTCGAACAAGGGCAACATCCATTACGCCATGGATGACGCCGACCGGGCCCGGATCATGAAGGAACACTTCAAGGGCAA
CCAGAAGGTCGAGATGACGCGCTTCAAGGGTCTGGGCGAAATGAACCCCGCCCAGCTCAAAGAAACCACCATGAACCCGT
CCAGCCGCACGCTGGCGCGCGTCACGCTCCCGGCGGCAATGGATGATCTCGAAATCAATCCCGCCGACCTCATCAACACC
CTGATGGGCAAAAAAGCCGAACTCCGCTTCCGCTTCATCCAGGAAAACGCCGCCTTCGTCGAAGAGCTCGACATCTAG

Upstream 100 bases:

>100_bases
AGAAAGCCGAAGGTTAATGCGCCGTTAAGGCTTTAACCTCCTATCTGCCACGACAGTTCCGCCGGGTCATCGGCGGCTTT
CAGTGACGGAGAAGCAGTCC

Downstream 100 bases:

>100_bases
GCTCAGGCTCACCGCGCCCTCTCCCCACAGCGTTTGCGCGTGGCTGCGGCAAGCACCAACCCAGTCAGCGAACGCGCCGT
CAGGTCTGCCGGATGAATTT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MSSARQMKQDDLLAGTAGYSAKDIEVLEGLEPVRKRPGMYIGGTDERAWHHLFAEVLDNAMDEAVAGYANRIDIKLDADG
FLTVVDNGRGIPIDPHPKFPKKSALEVIMTTLHSGGKFSDKAYSTAGGLHGVGISVVNALSELVEVEVARDRTLYRQDFS
RGLPLGKLQKVGAAPNRRGTSVRFKPDFQIFGDKLRWRPQRLFQMARSKAYLFRGVEVRWSCDPELLPEDSKVPAEAVLS
YPNGLADQLTEVFGDKSTITETPFTGLVDMGAEGKVEWAIAWTQAGFGEADGFARSYCNTIPTPEGGTHEAGFRSAITKG
IRNFGDLTGNKKAAEVTAEDVMGHSGLLLSVFIRGPEFVGQTKDKLSSTHAFRLVENAVRDHFDHWLAGSPKEANKLLGW
AIDRADERAKRRKAKEISRKSATKKLRLPGKLADCSAKGPEGTELFLVEGDSAGGSAKQARNRETQAILPLRGKILNVES
ASDDKLMGNQELADLSLALGTELGRKFNIDDLRYERIIIMTDADVDGAHIAALLITFFYRLTPGLIESGRLYLALPPLFK
LSNKGNIHYAMDDADRARIMKEHFKGNQKVEMTRFKGLGEMNPAQLKETTMNPSSRTLARVTLPAAMDDLEINPADLINT
LMGKKAELRFRFIQENAAFVEELDI

Sequences:

>Translated_665_residues
MSSARQMKQDDLLAGTAGYSAKDIEVLEGLEPVRKRPGMYIGGTDERAWHHLFAEVLDNAMDEAVAGYANRIDIKLDADG
FLTVVDNGRGIPIDPHPKFPKKSALEVIMTTLHSGGKFSDKAYSTAGGLHGVGISVVNALSELVEVEVARDRTLYRQDFS
RGLPLGKLQKVGAAPNRRGTSVRFKPDFQIFGDKLRWRPQRLFQMARSKAYLFRGVEVRWSCDPELLPEDSKVPAEAVLS
YPNGLADQLTEVFGDKSTITETPFTGLVDMGAEGKVEWAIAWTQAGFGEADGFARSYCNTIPTPEGGTHEAGFRSAITKG
IRNFGDLTGNKKAAEVTAEDVMGHSGLLLSVFIRGPEFVGQTKDKLSSTHAFRLVENAVRDHFDHWLAGSPKEANKLLGW
AIDRADERAKRRKAKEISRKSATKKLRLPGKLADCSAKGPEGTELFLVEGDSAGGSAKQARNRETQAILPLRGKILNVES
ASDDKLMGNQELADLSLALGTELGRKFNIDDLRYERIIIMTDADVDGAHIAALLITFFYRLTPGLIESGRLYLALPPLFK
LSNKGNIHYAMDDADRARIMKEHFKGNQKVEMTRFKGLGEMNPAQLKETTMNPSSRTLARVTLPAAMDDLEINPADLINT
LMGKKAELRFRFIQENAAFVEELDI
>Mature_664_residues
SSARQMKQDDLLAGTAGYSAKDIEVLEGLEPVRKRPGMYIGGTDERAWHHLFAEVLDNAMDEAVAGYANRIDIKLDADGF
LTVVDNGRGIPIDPHPKFPKKSALEVIMTTLHSGGKFSDKAYSTAGGLHGVGISVVNALSELVEVEVARDRTLYRQDFSR
GLPLGKLQKVGAAPNRRGTSVRFKPDFQIFGDKLRWRPQRLFQMARSKAYLFRGVEVRWSCDPELLPEDSKVPAEAVLSY
PNGLADQLTEVFGDKSTITETPFTGLVDMGAEGKVEWAIAWTQAGFGEADGFARSYCNTIPTPEGGTHEAGFRSAITKGI
RNFGDLTGNKKAAEVTAEDVMGHSGLLLSVFIRGPEFVGQTKDKLSSTHAFRLVENAVRDHFDHWLAGSPKEANKLLGWA
IDRADERAKRRKAKEISRKSATKKLRLPGKLADCSAKGPEGTELFLVEGDSAGGSAKQARNRETQAILPLRGKILNVESA
SDDKLMGNQELADLSLALGTELGRKFNIDDLRYERIIIMTDADVDGAHIAALLITFFYRLTPGLIESGRLYLALPPLFKL
SNKGNIHYAMDDADRARIMKEHFKGNQKVEMTRFKGLGEMNPAQLKETTMNPSSRTLARVTLPAAMDDLEINPADLINTL
MGKKAELRFRFIQENAAFVEELDI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=625, Percent_Identity=25.28, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI19913408, Length=627, Percent_Identity=24.5614035087719, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1789408, Length=650, Percent_Identity=42.9230769230769, Blast_Score=470, Evalue=1e-133,
Organism=Escherichia coli, GI48994957, Length=563, Percent_Identity=43.5168738898757, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17535065, Length=582, Percent_Identity=22.8522336769759, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=552, Percent_Identity=24.0942028985507, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=228, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=650, Percent_Identity=26, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI17136538, Length=629, Percent_Identity=25.5961844197138, Blast_Score=121, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: 5.99.1.-

Molecular weight: Translated: 73224; Mature: 73093

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSARQMKQDDLLAGTAGYSAKDIEVLEGLEPVRKRPGMYIGGTDERAWHHLFAEVLDNA
CCCHHHHCHHCCEECCCCCCCCHHHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHHHHHH
MDEAVAGYANRIDIKLDADGFLTVVDNGRGIPIDPHPKFPKKSALEVIMTTLHSGGKFSD
HHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
KAYSTAGGLHGVGISVVNALSELVEVEVARDRTLYRQDFSRGLPLGKLQKVGAAPNRRGT
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
SVRFKPDFQIFGDKLRWRPQRLFQMARSKAYLFRGVEVRWSCDPELLPEDSKVPAEAVLS
EEEECCCHHHHCCHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHH
YPNGLADQLTEVFGDKSTITETPFTGLVDMGAEGKVEWAIAWTQAGFGEADGFARSYCNT
CCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCEEEEEEEECCCCCCCCHHHHHHHCC
IPTPEGGTHEAGFRSAITKGIRNFGDLTGNKKAAEVTAEDVMGHSGLLLSVFIRGPEFVG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHC
QTKDKLSSTHAFRLVENAVRDHFDHWLAGSPKEANKLLGWAIDRADERAKRRKAKEISRK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SATKKLRLPGKLADCSAKGPEGTELFLVEGDSAGGSAKQARNRETQAILPLRGKILNVES
HHHHEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCEEEECCCCEEEEECC
ASDDKLMGNQELADLSLALGTELGRKFNIDDLRYERIIIMTDADVDGAHIAALLITFFYR
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
LTPGLIESGRLYLALPPLFKLSNKGNIHYAMDDADRARIMKEHFKGNQKVEMTRFKGLGE
HCCCHHHCCCEEEEECCHHEECCCCCEEEEECCHHHHHHHHHHCCCCCEEEEHHHCCCCC
MNPAQLKETTMNPSSRTLARVTLPAAMDDLEINPADLINTLMGKKAELRFRFIQENAAFV
CCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHEEEEHHHCHHHH
EELDI
HHCCC
>Mature Secondary Structure 
SSARQMKQDDLLAGTAGYSAKDIEVLEGLEPVRKRPGMYIGGTDERAWHHLFAEVLDNA
CCHHHHCHHCCEECCCCCCCCHHHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHHHHHH
MDEAVAGYANRIDIKLDADGFLTVVDNGRGIPIDPHPKFPKKSALEVIMTTLHSGGKFSD
HHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
KAYSTAGGLHGVGISVVNALSELVEVEVARDRTLYRQDFSRGLPLGKLQKVGAAPNRRGT
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
SVRFKPDFQIFGDKLRWRPQRLFQMARSKAYLFRGVEVRWSCDPELLPEDSKVPAEAVLS
EEEECCCHHHHCCHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHH
YPNGLADQLTEVFGDKSTITETPFTGLVDMGAEGKVEWAIAWTQAGFGEADGFARSYCNT
CCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCEEEEEEEECCCCCCCCHHHHHHHCC
IPTPEGGTHEAGFRSAITKGIRNFGDLTGNKKAAEVTAEDVMGHSGLLLSVFIRGPEFVG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHC
QTKDKLSSTHAFRLVENAVRDHFDHWLAGSPKEANKLLGWAIDRADERAKRRKAKEISRK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SATKKLRLPGKLADCSAKGPEGTELFLVEGDSAGGSAKQARNRETQAILPLRGKILNVES
HHHHEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCEEEECCCCEEEEECC
ASDDKLMGNQELADLSLALGTELGRKFNIDDLRYERIIIMTDADVDGAHIAALLITFFYR
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
LTPGLIESGRLYLALPPLFKLSNKGNIHYAMDDADRARIMKEHFKGNQKVEMTRFKGLGE
HCCCHHHCCCEEEEECCHHEECCCCCEEEEECCHHHHHHHHHHCCCCCEEEEHHHCCCCC
MNPAQLKETTMNPSSRTLARVTLPAAMDDLEINPADLINTLMGKKAELRFRFIQENAAFV
CCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHEEEEHHHCHHHH
EELDI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647; 9426128 [H]