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The map label for this gene is lptD [H]

Identifier: 114798255

GI number: 114798255

Start: 2236584

End: 2238794

Strand: Direct

Name: lptD [H]

Synonym: HNE_2149

Alternate gene names: 114798255

Gene position: 2236584-2238794 (Clockwise)

Preceding gene: 114799220

Following gene: 114797858

Centisome position: 60.37

GC content: 61.1

Gene sequence:

>2211_bases
ATGCCGTCTGTGGTGAAATGGTCTCACTATGCGCTTCTGGCCGGCTTTTCGGCCGTCCTGCCGGTCGCGGCCTATGCGCA
GACCGAGGTTGAAGCTGAAGCCGAACCTGAAGCCGCCACTACAGTCACAGACGAGCGCGTCGTGCTGGAAGCCGACATCG
TTTACGAAAACTCCGCTGACAACACGATCATCGCCGAAGGGAATGTCGAAGCGCTCCATCAGGGCCGCGTCCTGCGTGCC
GACCGGCTGATCTATGACCGCACCACCGAGCGGGCCCGCGCATCCGGCAATGTCATCATCACCGAGGCAGACGGCAGCCA
GCAATTTGCCGAGGAAGTCGATGTCGGCCCGGACCTGACAGACGGTTACGCCATCGGCTACTCCGCCCGCCTGCCCGGCG
ATGCCACTGTGGCCGCCAAATCCGCCATCCGGCGCTCCGACGGCATCAATGCTCTGGAACACGTCGTCTACACGGCCTGC
CCACTGTGTGAAGAAGACACGACGCCAACCTGGTCGATTCGGGCGCGCCGCGCGGTTCTCGACGAAGAATCGCAGATGAT
TTCCTATCGCGACGCCGTCATCGAAATTGGCGGCGTTCCGGTGTTTTATCTGCCTTACCTGTCTCATCCGGACCCCAATT
CGGAGCGCCGCTCCGGCCTGCTGATGCCCAATGCGGGCCTCTCGTCCACGCTCGGCGCCTTCTACAAACAGCCCTATTAC
TGGGCAGTCTCGGACCATTCCGACATCACCATTTCACCCATGGTGATGCAGAATGTGAACCCGCTGCTCGGCGTGGATTT
CCGGAAACGCTTCTATTCCGGGGCTGTGAAATTCGAGACCAGCTTCACACATGAAGCCGATTTTGATAGTGACGGCGAAA
CCTTCGGCGAAGAGACTTTCCGCGGGCATCTCTATGGCAACGGCCTGTTTGCCATCTCGCCGGAATGGAAATGGGGCTTC
GGCGTCGAAACCCAGACCGACGACCTTTACGACCGCCGCTACGACATCAGCGGACAGGGCGACAAGCGCGGCCTGTACTC
AAACCAGCCGCGCCGCCTGCTCTCGCAGGTCTTCGTCACCGGCCAGGGCGACAGCTACTACACCGACGCGGCGCTTCTGA
ACTTCCAGGGCCTGCGCGGCGAAGATGATGCCGCCCGCCTGCCACTGGTATCGCCGCTCGCCTACGCCGATAAATACTGG
GATCTCGGGGCATATGGCTTCGCGTCTGTTACCGCTTCGACCGCCATCCTGACCCGCGAAGTGGGCGCTGACAGCCACCG
CGCCAGCCTCGGCGGCGACTGGAACACGATGAAAATCCTGCCCGGCGGCTTCACCTTCGAGCCCTTCGCGGAGCTGCGCG
GCGATTACTACATGCTCGACGAGGATGTCAGCGGGAAGGACTCGGTCGCCCGCGCCGTCGGCAATACGGGCGCAAAACTC
GCCTATCCAATGGTGCGTCCAGGCACCAATGTCGATCTGATGCTGGAACCTGCCGTCATGGCGGCCTGGGGCTTTTCGAA
TGTGAATGACCCGGCGATCCCGGTCGAAGACAGCCTGCTCTACGAATTCGACGAATCGAGCCTGTTTGAAGCCAATGGCT
TCGGCAATTTCGACCTCTACGAAGGCGACGGAAAGGTCAGCGCCGGCCTGACAGCGCGGGCACTCTGGAAGAACGGCACG
GAAGTTTCCACCACGGTTGGACGCCGCTGGCGCTCTCGCGCGGACCCGGCATTCGATGTTGCCAGCAACCTTGATGGCAA
ATCGTCCGACTGGTTGGCCTCGGCGAGCCTCAAGATGGGCAAAAAGCTGGAGCTCAGCTCACGGGTGCGCCTCGACGAGA
AAGACCTCTCGCTGAGCCGCTTCGACATGCGCGCCGCCACCTCATTCGACTGGTTCCGCGCTGTCGGGCAGTATTACAAG
ATCGACGAGCGTATCAGCCCCGCCGGAACTTCGGACGAAGGCATTTTTCTGCGCGGTGAGATCCAGTTTACCGACCGCTA
CTCCGTTGTCTTCGGCCAGCTTCGGGACATCAGCCGGAATGTGAACACGCGGCAGGAAATCGGTATTGCCTACACAGATG
ACTGCTCGCGGTTTGAGCTGCTGTACAACCGCTCGGAGTTGCGAGACCGGACCCTGGGCCCAACCGAAAGCTTCCAGGTC
CGCTTTTCGCTGTTGTCTCTGGGCAATTTCGGGTCTGACAGCTTCGAATAA

Upstream 100 bases:

>100_bases
TGGATGGCTGGACGCCCCGGCGGTTCCTCTCTAAGCCTCATTTTTCGGGAGAAACGCGGCGCCAACTGCCGGGTGGGGCG
TTGTGTACGGGTAGTTCAGG

Downstream 100 bases:

>100_bases
TGTGAAATTTCAAAACAAACGAAGTGCAGCAATCTTAGCTGGTGACTGTAGCGGCACGTTGCACGATGAAACGAGACTTG
TAAGGTCCGGAAGCGAAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 736; Mature: 735

Protein sequence:

>736_residues
MPSVVKWSHYALLAGFSAVLPVAAYAQTEVEAEAEPEAATTVTDERVVLEADIVYENSADNTIIAEGNVEALHQGRVLRA
DRLIYDRTTERARASGNVIITEADGSQQFAEEVDVGPDLTDGYAIGYSARLPGDATVAAKSAIRRSDGINALEHVVYTAC
PLCEEDTTPTWSIRARRAVLDEESQMISYRDAVIEIGGVPVFYLPYLSHPDPNSERRSGLLMPNAGLSSTLGAFYKQPYY
WAVSDHSDITISPMVMQNVNPLLGVDFRKRFYSGAVKFETSFTHEADFDSDGETFGEETFRGHLYGNGLFAISPEWKWGF
GVETQTDDLYDRRYDISGQGDKRGLYSNQPRRLLSQVFVTGQGDSYYTDAALLNFQGLRGEDDAARLPLVSPLAYADKYW
DLGAYGFASVTASTAILTREVGADSHRASLGGDWNTMKILPGGFTFEPFAELRGDYYMLDEDVSGKDSVARAVGNTGAKL
AYPMVRPGTNVDLMLEPAVMAAWGFSNVNDPAIPVEDSLLYEFDESSLFEANGFGNFDLYEGDGKVSAGLTARALWKNGT
EVSTTVGRRWRSRADPAFDVASNLDGKSSDWLASASLKMGKKLELSSRVRLDEKDLSLSRFDMRAATSFDWFRAVGQYYK
IDERISPAGTSDEGIFLRGEIQFTDRYSVVFGQLRDISRNVNTRQEIGIAYTDDCSRFELLYNRSELRDRTLGPTESFQV
RFSLLSLGNFGSDSFE

Sequences:

>Translated_736_residues
MPSVVKWSHYALLAGFSAVLPVAAYAQTEVEAEAEPEAATTVTDERVVLEADIVYENSADNTIIAEGNVEALHQGRVLRA
DRLIYDRTTERARASGNVIITEADGSQQFAEEVDVGPDLTDGYAIGYSARLPGDATVAAKSAIRRSDGINALEHVVYTAC
PLCEEDTTPTWSIRARRAVLDEESQMISYRDAVIEIGGVPVFYLPYLSHPDPNSERRSGLLMPNAGLSSTLGAFYKQPYY
WAVSDHSDITISPMVMQNVNPLLGVDFRKRFYSGAVKFETSFTHEADFDSDGETFGEETFRGHLYGNGLFAISPEWKWGF
GVETQTDDLYDRRYDISGQGDKRGLYSNQPRRLLSQVFVTGQGDSYYTDAALLNFQGLRGEDDAARLPLVSPLAYADKYW
DLGAYGFASVTASTAILTREVGADSHRASLGGDWNTMKILPGGFTFEPFAELRGDYYMLDEDVSGKDSVARAVGNTGAKL
AYPMVRPGTNVDLMLEPAVMAAWGFSNVNDPAIPVEDSLLYEFDESSLFEANGFGNFDLYEGDGKVSAGLTARALWKNGT
EVSTTVGRRWRSRADPAFDVASNLDGKSSDWLASASLKMGKKLELSSRVRLDEKDLSLSRFDMRAATSFDWFRAVGQYYK
IDERISPAGTSDEGIFLRGEIQFTDRYSVVFGQLRDISRNVNTRQEIGIAYTDDCSRFELLYNRSELRDRTLGPTESFQV
RFSLLSLGNFGSDSFE
>Mature_735_residues
PSVVKWSHYALLAGFSAVLPVAAYAQTEVEAEAEPEAATTVTDERVVLEADIVYENSADNTIIAEGNVEALHQGRVLRAD
RLIYDRTTERARASGNVIITEADGSQQFAEEVDVGPDLTDGYAIGYSARLPGDATVAAKSAIRRSDGINALEHVVYTACP
LCEEDTTPTWSIRARRAVLDEESQMISYRDAVIEIGGVPVFYLPYLSHPDPNSERRSGLLMPNAGLSSTLGAFYKQPYYW
AVSDHSDITISPMVMQNVNPLLGVDFRKRFYSGAVKFETSFTHEADFDSDGETFGEETFRGHLYGNGLFAISPEWKWGFG
VETQTDDLYDRRYDISGQGDKRGLYSNQPRRLLSQVFVTGQGDSYYTDAALLNFQGLRGEDDAARLPLVSPLAYADKYWD
LGAYGFASVTASTAILTREVGADSHRASLGGDWNTMKILPGGFTFEPFAELRGDYYMLDEDVSGKDSVARAVGNTGAKLA
YPMVRPGTNVDLMLEPAVMAAWGFSNVNDPAIPVEDSLLYEFDESSLFEANGFGNFDLYEGDGKVSAGLTARALWKNGTE
VSTTVGRRWRSRADPAFDVASNLDGKSSDWLASASLKMGKKLELSSRVRLDEKDLSLSRFDMRAATSFDWFRAVGQYYKI
DERISPAGTSDEGIFLRGEIQFTDRYSVVFGQLRDISRNVNTRQEIGIAYTDDCSRFELLYNRSELRDRTLGPTESFQVR
FSLLSLGNFGSDSFE

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=194, Percent_Identity=28.8659793814433, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 81381; Mature: 81250

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVVKWSHYALLAGFSAVLPVAAYAQTEVEAEAEPEAATTVTDERVVLEADIVYENSAD
CCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEEEEEEECCCC
NTIIAEGNVEALHQGRVLRADRLIYDRTTERARASGNVIITEADGSQQFAEEVDVGPDLT
CEEEECCCHHHHHCCCEEEHHHEEECCCHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCC
DGYAIGYSARLPGDATVAAKSAIRRSDGINALEHVVYTACPLCEEDTTPTWSIRARRAVL
CCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHH
DEESQMISYRDAVIEIGGVPVFYLPYLSHPDPNSERRSGLLMPNAGLSSTLGAFYKQPYY
CCHHHHHHHHHEEEEECCCCEEEEECCCCCCCCHHHHCCEECCCCCCHHHHHHHHCCCEE
WAVSDHSDITISPMVMQNVNPLLGVDFRKRFYSGAVKFETSFTHEADFDSDGETFGEETF
EEECCCCCEEECHHHHHCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCHHCCHHE
RGHLYGNGLFAISPEWKWGFGVETQTDDLYDRRYDISGQGDKRGLYSNQPRRLLSQVFVT
EEEEEECEEEEECCCCCCCCCCCCCCCHHHHCEECCCCCCCCCCCCCCCHHHHHHHHEEE
GQGDSYYTDAALLNFQGLRGEDDAARLPLVSPLAYADKYWDLGAYGFASVTASTAILTRE
CCCCCEEECEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHEEEHH
VGADSHRASLGGDWNTMKILPGGFTFEPFAELRGDYYMLDEDVSGKDSVARAVGNTGAKL
CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEE
AYPMVRPGTNVDLMLEPAVMAAWGFSNVNDPAIPVEDSLLYEFDESSLFEANGFGNFDLY
EECEECCCCCEEEEECCHHHEEECCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCEEE
EGDGKVSAGLTARALWKNGTEVSTTVGRRWRSRADPAFDVASNLDGKSSDWLASASLKMG
CCCCCEECCCEEEHHHCCCCEEHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEHHHHHCC
KKLELSSRVRLDEKDLSLSRFDMRAATSFDWFRAVGQYYKIDERISPAGTSDEGIFLRGE
CEECCCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEE
IQFTDRYSVVFGQLRDISRNVNTRQEIGIAYTDDCSRFELLYNRSELRDRTLGPTESFQV
EEECCCHHHHHHHHHHHHCCCCCHHHCCEEECCCCCHHHHHHCHHHHHHCCCCCCCCEEE
RFSLLSLGNFGSDSFE
EEEEEECCCCCCCCCC
>Mature Secondary Structure 
PSVVKWSHYALLAGFSAVLPVAAYAQTEVEAEAEPEAATTVTDERVVLEADIVYENSAD
CCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEEEEEEEECCCC
NTIIAEGNVEALHQGRVLRADRLIYDRTTERARASGNVIITEADGSQQFAEEVDVGPDLT
CEEEECCCHHHHHCCCEEEHHHEEECCCHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCC
DGYAIGYSARLPGDATVAAKSAIRRSDGINALEHVVYTACPLCEEDTTPTWSIRARRAVL
CCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHH
DEESQMISYRDAVIEIGGVPVFYLPYLSHPDPNSERRSGLLMPNAGLSSTLGAFYKQPYY
CCHHHHHHHHHEEEEECCCCEEEEECCCCCCCCHHHHCCEECCCCCCHHHHHHHHCCCEE
WAVSDHSDITISPMVMQNVNPLLGVDFRKRFYSGAVKFETSFTHEADFDSDGETFGEETF
EEECCCCCEEECHHHHHCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCHHCCHHE
RGHLYGNGLFAISPEWKWGFGVETQTDDLYDRRYDISGQGDKRGLYSNQPRRLLSQVFVT
EEEEEECEEEEECCCCCCCCCCCCCCCHHHHCEECCCCCCCCCCCCCCCHHHHHHHHEEE
GQGDSYYTDAALLNFQGLRGEDDAARLPLVSPLAYADKYWDLGAYGFASVTASTAILTRE
CCCCCEEECEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHEEEHH
VGADSHRASLGGDWNTMKILPGGFTFEPFAELRGDYYMLDEDVSGKDSVARAVGNTGAKL
CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEE
AYPMVRPGTNVDLMLEPAVMAAWGFSNVNDPAIPVEDSLLYEFDESSLFEANGFGNFDLY
EECEECCCCCEEEEECCHHHEEECCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCEEE
EGDGKVSAGLTARALWKNGTEVSTTVGRRWRSRADPAFDVASNLDGKSSDWLASASLKMG
CCCCCEECCCEEEHHHCCCCEEHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEHHHHHCC
KKLELSSRVRLDEKDLSLSRFDMRAATSFDWFRAVGQYYKIDERISPAGTSDEGIFLRGE
CEECCCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEE
IQFTDRYSVVFGQLRDISRNVNTRQEIGIAYTDDCSRFELLYNRSELRDRTLGPTESFQV
EEECCCHHHHHHHHHHHHCCCCCHHHCCEEECCCCCHHHHHHCHHHHHHCCCCCCCCEEE
RFSLLSLGNFGSDSFE
EEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA