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The map label for this gene is sigK [H]

Identifier: 114798215

GI number: 114798215

Start: 3480520

End: 3481065

Strand: Reverse

Name: sigK [H]

Synonym: HNE_3328

Alternate gene names: 114798215

Gene position: 3481065-3480520 (Counterclockwise)

Preceding gene: 114800290

Following gene: 114799135

Centisome position: 93.96

GC content: 60.81

Gene sequence:

>546_bases
ATGCGGGAGCGGCATGCCGACCAGATGGCGGCTATTGCCGAACGCCAATGCCGGTCAGCTTTTGCCGAACTTTTCAGCTA
TTACGCGCCGCGCGTGAAGGGATTTATGCTCCGCCTTGGCGCGGCAGACGCTGAAGCAGAGGAGCTGTCGCAGGAAGTAA
TGATTGCGGTCTGGCAGAAGGCGGGGATGTATGACCGTTCCCAGGCATCTGTTTCGACCTGGATTTTCAGAATTGCCCGT
AATCGGCGCATTGATGCGCAGCGCCGTCAGCGGCGGCCGGAGCTCAGCGGGGATGATCCGATCCTGAGGCCCCCGGAAAT
CGAAACGCCTGACCAGGCCGTCTCGCGCGACCAGCTTGATACAGCGGTGCGCAAGCGGTTGACCTGCCTGCCTCAGGACC
AGCTCATCCTCATTCAGGCCGCTTTTTATGACGGCCTCTCCCATTCGGAAATTGCCCGTGCCTTCAACCTGCCGCTCGGG
ACGGTGAAGTCGCGCATTCGTCTCGCCTTCATGCGCCTGAAGGGCGAATTGGAGGGCGGCATCTAG

Upstream 100 bases:

>100_bases
CATGGTTGCTTTGCGGTTCATGAAGACAGACAATGAACGCACGTCCCCCCGAAAGGCCAAGCGTGTGTTCGATACCCCCG
TCCGGACAGCAGACCCGGTA

Downstream 100 bases:

>100_bases
CCGGGTAACTGCATTCTCCGGCGGCGTTTGCTTGACGAAACCCGAAACCGGACAATCTTGCCTGAAGGATACGGGACTTT
CTGACGATTATGACTTCTGA

Product: ECF subfamily RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-K factor [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MRERHADQMAAIAERQCRSAFAELFSYYAPRVKGFMLRLGAADAEAEELSQEVMIAVWQKAGMYDRSQASVSTWIFRIAR
NRRIDAQRRQRRPELSGDDPILRPPEIETPDQAVSRDQLDTAVRKRLTCLPQDQLILIQAAFYDGLSHSEIARAFNLPLG
TVKSRIRLAFMRLKGELEGGI

Sequences:

>Translated_181_residues
MRERHADQMAAIAERQCRSAFAELFSYYAPRVKGFMLRLGAADAEAEELSQEVMIAVWQKAGMYDRSQASVSTWIFRIAR
NRRIDAQRRQRRPELSGDDPILRPPEIETPDQAVSRDQLDTAVRKRLTCLPQDQLILIQAAFYDGLSHSEIARAFNLPLG
TVKSRIRLAFMRLKGELEGGI
>Mature_181_residues
MRERHADQMAAIAERQCRSAFAELFSYYAPRVKGFMLRLGAADAEAEELSQEVMIAVWQKAGMYDRSQASVSTWIFRIAR
NRRIDAQRRQRRPELSGDDPILRPPEIETPDQAVSRDQLDTAVRKRLTCLPQDQLILIQAAFYDGLSHSEIARAFNLPLG
TVKSRIRLAFMRLKGELEGGI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 20649; Mature: 20649

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERHADQMAAIAERQCRSAFAELFSYYAPRVKGFMLRLGAADAEAEELSQEVMIAVWQK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AGMYDRSQASVSTWIFRIARNRRIDAQRRQRRPELSGDDPILRPPEIETPDQAVSRDQLD
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TAVRKRLTCLPQDQLILIQAAFYDGLSHSEIARAFNLPLGTVKSRIRLAFMRLKGELEGG
HHHHHHHHCCCCCCEEEEHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
I
C
>Mature Secondary Structure
MRERHADQMAAIAERQCRSAFAELFSYYAPRVKGFMLRLGAADAEAEELSQEVMIAVWQK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AGMYDRSQASVSTWIFRIARNRRIDAQRRQRRPELSGDDPILRPPEIETPDQAVSRDQLD
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TAVRKRLTCLPQDQLILIQAAFYDGLSHSEIARAFNLPLGTVKSRIRLAFMRLKGELEGG
HHHHHHHHCCCCCCEEEEHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]