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The map label for this gene is murD [H]

Identifier: 114798168

GI number: 114798168

Start: 3194773

End: 3196188

Strand: Reverse

Name: murD [H]

Synonym: HNE_3026

Alternate gene names: 114798168

Gene position: 3196188-3194773 (Counterclockwise)

Preceding gene: 114797628

Following gene: 114799677

Centisome position: 86.27

GC content: 62.99

Gene sequence:

>1416_bases
ATGATCCCCATTACCGAATACGCTGGAAAAGACGTTGCGGTGTACGGCCTTGGCCGCTCCGGTCTCAGCGCGGCCAAAGC
CCTCAAGGCTGGCGGCGCGCGCGTCCATGCGTGGGACGATAATGAGGAAACCCGTGCCAAGGCGGAAGCTGCCGGCATTG
CCCTCTCCGATATCAACAAGCGTGACTGGCAGACCTTTGCCGCGCTCGTCCTGTCACCAGGCATTCCGTTCAAATTTCCC
CAGCCCCACCGCCTCGTTCGCATGGCGGAGATGACCGGCGTTCCGGTGATCGGCGACATGGAACTCTTTGCCCGCGCGGT
GCAGGCTCTGCCTGAGCGGGCGCGTCCGAAGGTTGTCGCTGTCACGGGCACGAACGGCAAATCGACGACCACCGCGCTGA
TCGGCCATATCCTGAAACAGGCTGGCCGGGACGCGCGCGTCGGCGGGAATATCGGCACCGGCGTGCTCGATCTCGCTGCC
CTGCATTCCAACGCCATCTATGTGCTGGAGCTTTCGTCCTACCAGCTTGATCTGGTCAAAAGCCTGCACTGCGATGTCAG
CGTCTTCATGAACCTCTCGCCCGATCACCTGGACCGGCATGGCGGGATGGATGGCTATCAGGCCGCCAAGATGCGCATTT
TCCAGAACCAGACGGACAAGAATGTCGCCGTCATTGGTTTTGACGACATCTACACCCAATCGATCGCCATTGGCCTGTCG
GCCAAGGGGCCGCAAAAGGTGGTGCAGATTTCCTCCACCTACACCCTCGGCAAGGGCATCAGCGCGGTTGATGGCCGCCT
TTATGACAACCAGTCCGGCAAGGCTGAATTTGTCGGCAAGCTGGATGAGTGTCCGGCGCTGATCGGGCGCCACAATCACC
AGAACGCAGCCGCTGCCTATGCCGCCTGCCGCGCGCTCGGCCTTGATCCGGCAACGATCATGGCGGGCCTGCGCACCTTC
CCTGGCCTCGCCCACCGGATGGAAGCAGTTGGCGAGCTCGACGGCATTCGCTTCATCAATGATTCCAAGGCAACCAACGC
CCAGGCCGCCGAACAGGCATTGCGCTCCTTCAAGAACATCTACTGGATCGCCGGGGGTGTTCCGAAGGCGGAGGGCATTG
CCCCGCTCGCATCGCTCTTTCCCAACGTCACCAAGGCCTACCTGATCGGCCAGGCCGAAGACGCCTTCGCCGCGACCCTG
AACGGCAAGGTGCCCTCACAGGTTTGCGGCTCGCTGGACCGCGCGGTTGAGGCCGCTTACCGCGACGCGAAAGCGGCCGG
CGAGCCCGGCGCCGTGATCCTGTTCTCGCCCGCCTGCGCCAGCTTTGACCAGTTCAAGGATTACGAACACCGGGGCGATG
CGTTCCGCACGCTCGTGCACGGCATTGTGCCGGCGAGCCTCAAGGTGACCGCGTGA

Upstream 100 bases:

>100_bases
AACTGGCCGGAAACGCGCGTTGTCGTGCGCTTCTGGATACTCTCCGTCCTGTTCGCCCTCGCGGGCCTCGCCACGCTGAA
GCTGAGGTAACCCGCGCGCC

Downstream 100 bases:

>100_bases
GCTATACGGCCAACGCACCGCTTCTGCCGCGTTCGGATACATCCTGGTTTACCGAATGGCGCCGCACGCTGGACTGGGGC
CTGGTGGCCGGCGCGGTTCT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MIPITEYAGKDVAVYGLGRSGLSAAKALKAGGARVHAWDDNEETRAKAEAAGIALSDINKRDWQTFAALVLSPGIPFKFP
QPHRLVRMAEMTGVPVIGDMELFARAVQALPERARPKVVAVTGTNGKSTTTALIGHILKQAGRDARVGGNIGTGVLDLAA
LHSNAIYVLELSSYQLDLVKSLHCDVSVFMNLSPDHLDRHGGMDGYQAAKMRIFQNQTDKNVAVIGFDDIYTQSIAIGLS
AKGPQKVVQISSTYTLGKGISAVDGRLYDNQSGKAEFVGKLDECPALIGRHNHQNAAAAYAACRALGLDPATIMAGLRTF
PGLAHRMEAVGELDGIRFINDSKATNAQAAEQALRSFKNIYWIAGGVPKAEGIAPLASLFPNVTKAYLIGQAEDAFAATL
NGKVPSQVCGSLDRAVEAAYRDAKAAGEPGAVILFSPACASFDQFKDYEHRGDAFRTLVHGIVPASLKVTA

Sequences:

>Translated_471_residues
MIPITEYAGKDVAVYGLGRSGLSAAKALKAGGARVHAWDDNEETRAKAEAAGIALSDINKRDWQTFAALVLSPGIPFKFP
QPHRLVRMAEMTGVPVIGDMELFARAVQALPERARPKVVAVTGTNGKSTTTALIGHILKQAGRDARVGGNIGTGVLDLAA
LHSNAIYVLELSSYQLDLVKSLHCDVSVFMNLSPDHLDRHGGMDGYQAAKMRIFQNQTDKNVAVIGFDDIYTQSIAIGLS
AKGPQKVVQISSTYTLGKGISAVDGRLYDNQSGKAEFVGKLDECPALIGRHNHQNAAAAYAACRALGLDPATIMAGLRTF
PGLAHRMEAVGELDGIRFINDSKATNAQAAEQALRSFKNIYWIAGGVPKAEGIAPLASLFPNVTKAYLIGQAEDAFAATL
NGKVPSQVCGSLDRAVEAAYRDAKAAGEPGAVILFSPACASFDQFKDYEHRGDAFRTLVHGIVPASLKVTA
>Mature_471_residues
MIPITEYAGKDVAVYGLGRSGLSAAKALKAGGARVHAWDDNEETRAKAEAAGIALSDINKRDWQTFAALVLSPGIPFKFP
QPHRLVRMAEMTGVPVIGDMELFARAVQALPERARPKVVAVTGTNGKSTTTALIGHILKQAGRDARVGGNIGTGVLDLAA
LHSNAIYVLELSSYQLDLVKSLHCDVSVFMNLSPDHLDRHGGMDGYQAAKMRIFQNQTDKNVAVIGFDDIYTQSIAIGLS
AKGPQKVVQISSTYTLGKGISAVDGRLYDNQSGKAEFVGKLDECPALIGRHNHQNAAAAYAACRALGLDPATIMAGLRTF
PGLAHRMEAVGELDGIRFINDSKATNAQAAEQALRSFKNIYWIAGGVPKAEGIAPLASLFPNVTKAYLIGQAEDAFAATL
NGKVPSQVCGSLDRAVEAAYRDAKAAGEPGAVILFSPACASFDQFKDYEHRGDAFRTLVHGIVPASLKVTA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=473, Percent_Identity=35.5179704016913, Blast_Score=204, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49955; Mature: 49955

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPITEYAGKDVAVYGLGRSGLSAAKALKAGGARVHAWDDNEETRAKAEAAGIALSDINK
CCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCEEHHCCCH
RDWQTFAALVLSPGIPFKFPQPHRLVRMAEMTGVPVIGDMELFARAVQALPERARPKVVA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCEEE
VTGTNGKSTTTALIGHILKQAGRDARVGGNIGTGVLDLAALHSNAIYVLELSSYQLDLVK
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHH
SLHCDVSVFMNLSPDHLDRHGGMDGYQAAKMRIFQNQTDKNVAVIGFDDIYTQSIAIGLS
HHCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHEEEEEC
AKGPQKVVQISSTYTLGKGISAVDGRLYDNQSGKAEFVGKLDECPALIGRHNHQNAAAAY
CCCCHHEEEEECCEECCCCCHHHCCEEECCCCCCHHHHCCHHHHHHHHCCCCCCHHHHHH
AACRALGLDPATIMAGLRTFPGLAHRMEAVGELDGIRFINDSKATNAQAAEQALRSFKNI
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCE
YWIAGGVPKAEGIAPLASLFPNVTKAYLIGQAEDAFAATLNGKVPSQVCGSLDRAVEAAY
EEEECCCCCCCCCHHHHHHCCCHHHHHEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
RDAKAAGEPGAVILFSPACASFDQFKDYEHRGDAFRTLVHGIVPASLKVTA
HHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MIPITEYAGKDVAVYGLGRSGLSAAKALKAGGARVHAWDDNEETRAKAEAAGIALSDINK
CCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCEEHHCCCH
RDWQTFAALVLSPGIPFKFPQPHRLVRMAEMTGVPVIGDMELFARAVQALPERARPKVVA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCEEE
VTGTNGKSTTTALIGHILKQAGRDARVGGNIGTGVLDLAALHSNAIYVLELSSYQLDLVK
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHH
SLHCDVSVFMNLSPDHLDRHGGMDGYQAAKMRIFQNQTDKNVAVIGFDDIYTQSIAIGLS
HHCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHEEEEEC
AKGPQKVVQISSTYTLGKGISAVDGRLYDNQSGKAEFVGKLDECPALIGRHNHQNAAAAY
CCCCHHEEEEECCEECCCCCHHHCCEEECCCCCCHHHHCCHHHHHHHHCCCCCCHHHHHH
AACRALGLDPATIMAGLRTFPGLAHRMEAVGELDGIRFINDSKATNAQAAEQALRSFKNI
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCE
YWIAGGVPKAEGIAPLASLFPNVTKAYLIGQAEDAFAATLNGKVPSQVCGSLDRAVEAAY
EEEECCCCCCCCCHHHHHHCCCHHHHHEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
RDAKAAGEPGAVILFSPACASFDQFKDYEHRGDAFRTLVHGIVPASLKVTA
HHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA