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The map label for this gene is purN [H]

Identifier: 114797986

GI number: 114797986

Start: 2367928

End: 2368512

Strand: Direct

Name: purN [H]

Synonym: HNE_2264

Alternate gene names: 114797986

Gene position: 2367928-2368512 (Clockwise)

Preceding gene: 114798932

Following gene: 114798803

Centisome position: 63.91

GC content: 65.64

Gene sequence:

>585_bases
ATGACGCGCCTCAATCTGGCGATCCTGATTTCAGGCCGCGGCTCCAATATGGAGGCCCTGCTGAGCGCCGCCGAAGACCC
GGCCTATCCGGCAAAGCCCGTTCTGGTCGCCTCCAACCGTCCCGATGCCAAGGGCCTTGAAACCGCCGCCGCTGCGGGCA
TCCCCACGCTCTCCATCGACCACAAGCTCTATGGCAAGGACCGCGAAGCCTTCGAGCGCGCGCTGGACGAGGCCTTGACT
AAAGCTGGCACGGAGATCATCGCGCTGGCCGGTTTCATGCGCGTGCTCACCCCTTGGTTCGTAATGCGCTGGGAAGGCCG
GATGATCAACATCCACCCATCGCTCCTGCCGAAATACAAAGGCCTCGACACGCATCAGCGCGCCATCGATGCAGGCGACG
CGGAAGCAGGCTGCACGGTTCACTGGGTCAGCGCCGGGGTCGATGAAGGCGAGATCATCGCGCAGGCCTCCGTGCCCATC
CTGCCGGGCGACACCGCAGACACCCTCGCCGCCCGCACCCTGCCGGAAGAACACACACTCTACCCGCGCGCCCTCGCGCT
GGCCTGCCAGCAAATCCGGCGCTAG

Upstream 100 bases:

>100_bases
GCCTTGTTCTCGCCGTCGCGCCCGAAGAGGCAGACGCCGTGATCGCAGACCTGAAAGCGGCGGGCGAAGACGCGCGCGTT
CTCGGTGAGCTTGCCAGCGC

Downstream 100 bases:

>100_bases
CGCAAAGCCCCTTGCCCCGATGTGGACATGGGGGTGCCCAGCAGCTATCCCCGAAGCCATACCCAGTGCGGGTTCACCGC
ACGGATTTACGGCACAGGGA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MTRLNLAILISGRGSNMEALLSAAEDPAYPAKPVLVASNRPDAKGLETAAAAGIPTLSIDHKLYGKDREAFERALDEALT
KAGTEIIALAGFMRVLTPWFVMRWEGRMINIHPSLLPKYKGLDTHQRAIDAGDAEAGCTVHWVSAGVDEGEIIAQASVPI
LPGDTADTLAARTLPEEHTLYPRALALACQQIRR

Sequences:

>Translated_194_residues
MTRLNLAILISGRGSNMEALLSAAEDPAYPAKPVLVASNRPDAKGLETAAAAGIPTLSIDHKLYGKDREAFERALDEALT
KAGTEIIALAGFMRVLTPWFVMRWEGRMINIHPSLLPKYKGLDTHQRAIDAGDAEAGCTVHWVSAGVDEGEIIAQASVPI
LPGDTADTLAARTLPEEHTLYPRALALACQQIRR
>Mature_193_residues
TRLNLAILISGRGSNMEALLSAAEDPAYPAKPVLVASNRPDAKGLETAAAAGIPTLSIDHKLYGKDREAFERALDEALTK
AGTEIIALAGFMRVLTPWFVMRWEGRMINIHPSLLPKYKGLDTHQRAIDAGDAEAGCTVHWVSAGVDEGEIIAQASVPIL
PGDTADTLAARTLPEEHTLYPRALALACQQIRR

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=185, Percent_Identity=46.4864864864865, Blast_Score=177, Evalue=4e-45,
Organism=Homo sapiens, GI209869995, Length=185, Percent_Identity=46.4864864864865, Blast_Score=177, Evalue=4e-45,
Organism=Homo sapiens, GI209869993, Length=185, Percent_Identity=46.4864864864865, Blast_Score=177, Evalue=4e-45,
Organism=Escherichia coli, GI1788846, Length=183, Percent_Identity=42.6229508196721, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1787483, Length=189, Percent_Identity=28.042328042328, Blast_Score=73, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=190, Percent_Identity=40.5263157894737, Blast_Score=145, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6320616, Length=212, Percent_Identity=28.7735849056604, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=51.3368983957219, Blast_Score=182, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20891; Mature: 20760

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLNLAILISGRGSNMEALLSAAEDPAYPAKPVLVASNRPDAKGLETAAAAGIPTLSID
CCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEC
HKLYGKDREAFERALDEALTKAGTEIIALAGFMRVLTPWFVMRWEGRMINIHPSLLPKYK
CHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEECHHHCCCCC
GLDTHQRAIDAGDAEAGCTVHWVSAGVDEGEIIAQASVPILPGDTADTLAARTLPEEHTL
CCCHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCHHCC
YPRALALACQQIRR
CHHHHHHHHHHHCC
>Mature Secondary Structure 
TRLNLAILISGRGSNMEALLSAAEDPAYPAKPVLVASNRPDAKGLETAAAAGIPTLSID
CCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEC
HKLYGKDREAFERALDEALTKAGTEIIALAGFMRVLTPWFVMRWEGRMINIHPSLLPKYK
CHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEECHHHCCCCC
GLDTHQRAIDAGDAEAGCTVHWVSAGVDEGEIIAQASVPILPGDTADTLAARTLPEEHTL
CCCHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCHHCC
YPRALALACQQIRR
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]