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The map label for this gene is tag [C]

Identifier: 114797489

GI number: 114797489

Start: 163771

End: 164445

Strand: Direct

Name: tag [C]

Synonym: HNE_0175

Alternate gene names: 114797489

Gene position: 163771-164445 (Clockwise)

Preceding gene: 114798252

Following gene: 114798757

Centisome position: 4.42

GC content: 57.19

Gene sequence:

>675_bases
ATGAAGCGCCGCTTCGCTCCCATACTCAAGATGGCCGCCAGCCATCATGGTGGGGAAGAGGCGGCGCTTGCAAAATCGGT
GAACGCCCATTCGGTGAAGCGTCTCGAGAAAGTACCGGGAGACAGGCTGCTTTCTATCATGACCCGGTGTGTCTTCAATG
CGGGCTTCAACTGGAAAGTCATCGAGGCTAAATGGGAAGGGTTCGAAACAGCCTTTGAGGGGTTTGACCCAGCCAAGCTT
GCCTTTTTCGGCGACGAGATGATGGACCATCTTGTTACGGATGAACGCATTGTCCGCAATGGGCAGAAGATCAGAGCCAC
CCTTGAGAACGCAAAGTTTGTTGCGGAGTTCGAGAACAATCAGGGTGGTTTTGGTCGTTTTCTTGCCGGCTGGCCCGGTG
AGGATCAGGCCGGACTTCTAGACGTCCTCAACAAGCGCGGCTCCCGGTTGGGCGGGGCAACGGGTCAATACTTCCTGCGC
TTCGCAGGATGGGACGCCTGGATCGCATCGTCTCATGTGTGCGCGGCATTGGTGCGCGAAGGCGTGCTCGACAAACCCGT
TGCCACAAGCAAACGTGATCTGGCGAGCCTTCAGTCGGCGATCAACGCTTACCATGACGACAGTGGGCTGCCTCGTGCCC
AGATCAGCCGGTTGCTTGCGATGAGCGTTGGCTAA

Upstream 100 bases:

>100_bases
GTATGCCCTGGTGGATCTTGCCCATGCAGGAAATCCACGTCCGATGAGCGCGGATGATTATGCGGCGGTATATGAGACCG
CGCTCGGCTGAGGAAGAACC

Downstream 100 bases:

>100_bases
CGGACCTTATTCGGCATTCAGCTCAGTAAGGCCAAGTGCCGCTTCAACATCCGCCCAGTTCACAATCTTGAAGTTCTGGT
CCTGACCCGACCGCGGATTA

Product: hypothetical protein

Products: Methyladenine or Methylguanine [C]

Alternate protein names: DNA-3-Methyladenine Glycosylase; 3-Methyladenine DNA Glycosylase-Like Protein; 3-Methyl-Adenine DNA Glycosylase; DNA Glycosylase Protein; DNA-3-Methyladenine Glycosylase I; DNA-Glycosylase

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKRRFAPILKMAASHHGGEEAALAKSVNAHSVKRLEKVPGDRLLSIMTRCVFNAGFNWKVIEAKWEGFETAFEGFDPAKL
AFFGDEMMDHLVTDERIVRNGQKIRATLENAKFVAEFENNQGGFGRFLAGWPGEDQAGLLDVLNKRGSRLGGATGQYFLR
FAGWDAWIASSHVCAALVREGVLDKPVATSKRDLASLQSAINAYHDDSGLPRAQISRLLAMSVG

Sequences:

>Translated_224_residues
MKRRFAPILKMAASHHGGEEAALAKSVNAHSVKRLEKVPGDRLLSIMTRCVFNAGFNWKVIEAKWEGFETAFEGFDPAKL
AFFGDEMMDHLVTDERIVRNGQKIRATLENAKFVAEFENNQGGFGRFLAGWPGEDQAGLLDVLNKRGSRLGGATGQYFLR
FAGWDAWIASSHVCAALVREGVLDKPVATSKRDLASLQSAINAYHDDSGLPRAQISRLLAMSVG
>Mature_224_residues
MKRRFAPILKMAASHHGGEEAALAKSVNAHSVKRLEKVPGDRLLSIMTRCVFNAGFNWKVIEAKWEGFETAFEGFDPAKL
AFFGDEMMDHLVTDERIVRNGQKIRATLENAKFVAEFENNQGGFGRFLAGWPGEDQAGLLDVLNKRGSRLGGATGQYFLR
FAGWDAWIASSHVCAALVREGVLDKPVATSKRDLASLQSAINAYHDDSGLPRAQISRLLAMSVG

Specific function: Hydrolysis Of The Deoxyribose N-Glycosidic Bond To Excise 3-Methyladenine From The Damaged DNA Polymer Formed By Alkylation Lesions. [C]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.2.20 [C]

Molecular weight: Translated: 24609; Mature: 24609

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRFAPILKMAASHHGGEEAALAKSVNAHSVKRLEKVPGDRLLSIMTRCVFNAGFNWKV
CCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
IEAKWEGFETAFEGFDPAKLAFFGDEMMDHLVTDERIVRNGQKIRATLENAKFVAEFENN
EEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCC
QGGFGRFLAGWPGEDQAGLLDVLNKRGSRLGGATGQYFLRFAGWDAWIASSHVCAALVRE
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHC
GVLDKPVATSKRDLASLQSAINAYHDDSGLPRAQISRLLAMSVG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKRRFAPILKMAASHHGGEEAALAKSVNAHSVKRLEKVPGDRLLSIMTRCVFNAGFNWKV
CCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
IEAKWEGFETAFEGFDPAKLAFFGDEMMDHLVTDERIVRNGQKIRATLENAKFVAEFENN
EEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCC
QGGFGRFLAGWPGEDQAGLLDVLNKRGSRLGGATGQYFLRFAGWDAWIASSHVCAALVRE
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHC
GVLDKPVATSKRDLASLQSAINAYHDDSGLPRAQISRLLAMSVG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 3-Methylguanine; Adenine; Caffeine [C]

Metal ions: Ca2+; Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.000014 {3-methyladenine}} 0.000006 {3-methyladenine}} [C]

Substrates: Alkylated DNA; DNA [C]

Specific reaction: Hydrolysis of alkylated DNA releasing methyladenine or methylguanine Protein + DNA = Protein-DNA [C]

General reaction: Hydrolysis of N-glycosyl compounds [C]

Inhibitor: 3-Methyl adenine; DNA with a purinic sites; Double-stranded DNA; FeCl3; KCl; NaCl; NEM; p-Hydroxy mercuribenzoate; p-Substituted mercuribenzoate; Spermidine; ZnSO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA