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The map label for this gene is rimK1 [H]

Identifier: 114797472

GI number: 114797472

Start: 3295048

End: 3295962

Strand: Reverse

Name: rimK1 [H]

Synonym: HNE_3114

Alternate gene names: 114797472

Gene position: 3295962-3295048 (Counterclockwise)

Preceding gene: 114797164

Following gene: 114798945

Centisome position: 88.96

GC content: 66.45

Gene sequence:

>915_bases
GTGAAGCGCCCGCTGCGCATTGCGCTGCTGACGCGCGAGCCGAAAAACTACTCCTCCCGGCGCCTGATCGAGGCAGCCGA
AAAGCGCGGCCATGTGATCGAGGTGATCGATACCGCCCGCTGCTATATGCGCATCGGCGCGATAGACGCCGAGGTGCATT
ATGATGGCCAGGCGCTGCCCAGGTATGACGCGGTGATCCCTCGTATCGGCGCGACGATTACAGACTATGGCATGGCGGTG
CTGCGCCAGTTTTCAAACACCGGCGCCTTCTGCCTCAACACGGCCGGCGCCATCGGCCGTTCACGTGACAAGCTGCTCGC
CCATCAGATGCTCGCGCGGGCGAAGATCGCCATGCCGATCACGGCGTTCGCGCACAGCCCGAAGGATACCGCCGGTCTCG
TCGCGCTGGCGGGCGGGGCGCCGATCGTGCTCAAGCTGCTCTCCTCCACCCAAGGGAAGGGCGTGCTGCTGGCGGAGTCT
CGCAAGTCTGCCGAGCGGCTGGTCGATGCCTTCCGGGGGCTCGACGCAAACTTCCTGGTGCAGGAATTTGTCGAGGAGGC
GGGCGGCGCGGATGTGCGCGCCTTTGTTGTGGGCGGCAAGGTGGTGGGCGCCATGCGCCGGCAGGCGGAGACGGGCGAGT
TCCGGTCAAACCTTCATCGCGGCGGCACGGCGAGCGAAGCCAAACTCTCCCCGCAGGAGCGGGACGTGGCGCGCGAAGCG
GCCAAGGTGCTGCGGCTGGAAGTGGCGGGCGTTGACCTCTTGCAGACGAAGGACGGCCCGAAGGTGCTGGAAGTCAACTC
CTCGCCCGGCCTCAAAGGCATCGAGAAGGCGACCGGCAAGGACATTGCCGCCCGTATCGTCGAGCATCTGGAGAAGGTCG
TGCGTCCGCTGTCGCGCCATCGGGAGCCGCTCTGA

Upstream 100 bases:

>100_bases
CGGCGCCATTCAGCCAAGCTGGGTGATTGATCCGAATGCGTCCTTTCTTCAGCCTCAGCTGAGCTTTCAGACCTATCAGG
GCCTGCCGCGCCGCAAGCCG

Downstream 100 bases:

>100_bases
CGCAGCCCTCAATGGCGGCTGAACAGGCGGCCCTTTTCCGTCACCAGGATCACCACCAGCGAAGCGGCGCCGAGGATCAC
GAAGCCGAGCGTGAGCGGAA

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKRPLRIALLTREPKNYSSRRLIEAAEKRGHVIEVIDTARCYMRIGAIDAEVHYDGQALPRYDAVIPRIGATITDYGMAV
LRQFSNTGAFCLNTAGAIGRSRDKLLAHQMLARAKIAMPITAFAHSPKDTAGLVALAGGAPIVLKLLSSTQGKGVLLAES
RKSAERLVDAFRGLDANFLVQEFVEEAGGADVRAFVVGGKVVGAMRRQAETGEFRSNLHRGGTASEAKLSPQERDVAREA
AKVLRLEVAGVDLLQTKDGPKVLEVNSSPGLKGIEKATGKDIAARIVEHLEKVVRPLSRHREPL

Sequences:

>Translated_304_residues
MKRPLRIALLTREPKNYSSRRLIEAAEKRGHVIEVIDTARCYMRIGAIDAEVHYDGQALPRYDAVIPRIGATITDYGMAV
LRQFSNTGAFCLNTAGAIGRSRDKLLAHQMLARAKIAMPITAFAHSPKDTAGLVALAGGAPIVLKLLSSTQGKGVLLAES
RKSAERLVDAFRGLDANFLVQEFVEEAGGADVRAFVVGGKVVGAMRRQAETGEFRSNLHRGGTASEAKLSPQERDVAREA
AKVLRLEVAGVDLLQTKDGPKVLEVNSSPGLKGIEKATGKDIAARIVEHLEKVVRPLSRHREPL
>Mature_304_residues
MKRPLRIALLTREPKNYSSRRLIEAAEKRGHVIEVIDTARCYMRIGAIDAEVHYDGQALPRYDAVIPRIGATITDYGMAV
LRQFSNTGAFCLNTAGAIGRSRDKLLAHQMLARAKIAMPITAFAHSPKDTAGLVALAGGAPIVLKLLSSTQGKGVLLAES
RKSAERLVDAFRGLDANFLVQEFVEEAGGADVRAFVVGGKVVGAMRRQAETGEFRSNLHRGGTASEAKLSPQERDVAREA
AKVLRLEVAGVDLLQTKDGPKVLEVNSSPGLKGIEKATGKDIAARIVEHLEKVVRPLSRHREPL

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=232, Percent_Identity=28.8793103448276, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI134254462, Length=219, Percent_Identity=31.9634703196347, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787076, Length=288, Percent_Identity=53.8194444444444, Blast_Score=329, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 32871; Mature: 32871

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPLRIALLTREPKNYSSRRLIEAAEKRGHVIEVIDTARCYMRIGAIDAEVHYDGQALP
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCEEEEEEECCCCCC
RYDAVIPRIGATITDYGMAVLRQFSNTGAFCLNTAGAIGRSRDKLLAHQMLARAKIAMPI
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHHCCH
TAFAHSPKDTAGLVALAGGAPIVLKLLSSTQGKGVLLAESRKSAERLVDAFRGLDANFLV
HHHHCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
QEFVEEAGGADVRAFVVGGKVVGAMRRQAETGEFRSNLHRGGTASEAKLSPQERDVAREA
HHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
AKVLRLEVAGVDLLQTKDGPKVLEVNSSPGLKGIEKATGKDIAARIVEHLEKVVRPLSRH
HHHHHHHHCCCEEEECCCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHC
REPL
CCCC
>Mature Secondary Structure
MKRPLRIALLTREPKNYSSRRLIEAAEKRGHVIEVIDTARCYMRIGAIDAEVHYDGQALP
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCEEEEEEECCCCCC
RYDAVIPRIGATITDYGMAVLRQFSNTGAFCLNTAGAIGRSRDKLLAHQMLARAKIAMPI
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHHCCH
TAFAHSPKDTAGLVALAGGAPIVLKLLSSTQGKGVLLAESRKSAERLVDAFRGLDANFLV
HHHHCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
QEFVEEAGGADVRAFVVGGKVVGAMRRQAETGEFRSNLHRGGTASEAKLSPQERDVAREA
HHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
AKVLRLEVAGVDLLQTKDGPKVLEVNSSPGLKGIEKATGKDIAARIVEHLEKVVRPLSRH
HHHHHHHHCCCEEEECCCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHC
REPL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA