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The map label for this gene is polA [H]

Identifier: 114797270

GI number: 114797270

Start: 187145

End: 187759

Strand: Direct

Name: polA [H]

Synonym: HNE_0202

Alternate gene names: 114797270

Gene position: 187145-187759 (Clockwise)

Preceding gene: 114798243

Following gene: 114799884

Centisome position: 5.05

GC content: 61.95

Gene sequence:

>615_bases
ATGAACCAGATCACCCTCCATAAGGGCGACCTTCCCGAAGGTCTGAGCCTTGGCCCCGTGATCGCCGTCGATACCGAGGC
GATGGGCCTGAACGCCATGCGCGACAATCTCACGCTCGTGCAGGTTTCCTCCGGGGATGGCACAGCGCATCTCGTGCAGC
TGACCCGCGACTATGACTGCCCCAATCTCAAGGCGCTGTTGACCGATCCCAAGGTGCTGAAGATTTTCCACTTCGCCCGG
TTTGATGTGGTGATGATGAAGCGCTGGATGGGGATCACCTGCGCGCCGATCTGGTGCACCAAGATCGCCTCGCGCCTGGC
GCGCACTTACACCGACCGGCACGGCCTGAAGGATGTGGCGCGCGAAGTGGCGGGCGTGGATATGTCCAAGGCGCAGCAGA
GCTCTGACTGGGGCCAGGACAAGCTTACCGACGCCCAGATCCAGTATGCGGCCAGTGATGTACTGTATCTCCACCAGATC
AAGGCGGGCCTGGAGGCCATGCTGGAGCGGGAAGGGCGCCTGGAACTTGCGCAGGCCTGCTTCGATTTCCTGCCGATCCG
GGCCGATCTCGATCTGGCCGGCTGGCCGGACGAGGACATTTTTGCTCACAGTTGA

Upstream 100 bases:

>100_bases
TTCCTGTCCGCGCTGGATGCGGCGATTGTGGCGGCCAAGCGAGCGGCGGGCTAGGCGCCTTTGACTTGTGCTTAATTCCG
GTCGTGCTAAGGGCCGATCC

Downstream 100 bases:

>100_bases
GCAAAGCCTGTCCGATACCTGCCTTCTCGTGCTTTCGCAGGCGCAGCTTCACGGTTAAGACTGCTGGTTGATCGCAGTTT
ATCCGGATTCTCCCCGCCTC

Product: putative exonuclease

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNQITLHKGDLPEGLSLGPVIAVDTEAMGLNAMRDNLTLVQVSSGDGTAHLVQLTRDYDCPNLKALLTDPKVLKIFHFAR
FDVVMMKRWMGITCAPIWCTKIASRLARTYTDRHGLKDVAREVAGVDMSKAQQSSDWGQDKLTDAQIQYAASDVLYLHQI
KAGLEAMLEREGRLELAQACFDFLPIRADLDLAGWPDEDIFAHS

Sequences:

>Translated_204_residues
MNQITLHKGDLPEGLSLGPVIAVDTEAMGLNAMRDNLTLVQVSSGDGTAHLVQLTRDYDCPNLKALLTDPKVLKIFHFAR
FDVVMMKRWMGITCAPIWCTKIASRLARTYTDRHGLKDVAREVAGVDMSKAQQSSDWGQDKLTDAQIQYAASDVLYLHQI
KAGLEAMLEREGRLELAQACFDFLPIRADLDLAGWPDEDIFAHS
>Mature_204_residues
MNQITLHKGDLPEGLSLGPVIAVDTEAMGLNAMRDNLTLVQVSSGDGTAHLVQLTRDYDCPNLKALLTDPKVLKIFHFAR
FDVVMMKRWMGITCAPIWCTKIASRLARTYTDRHGLKDVAREVAGVDMSKAQQSSDWGQDKLTDAQIQYAASDVLYLHQI
KAGLEAMLEREGRLELAQACFDFLPIRADLDLAGWPDEDIFAHS

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=170, Percent_Identity=31.7647058823529, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 22687; Mature: 22687

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQITLHKGDLPEGLSLGPVIAVDTEAMGLNAMRDNLTLVQVSSGDGTAHLVQLTRDYDC
CCCEEEECCCCCCCCCCCCEEEECCHHHCCHHHHCCEEEEEEECCCCCEEEEEEECCCCC
PNLKALLTDPKVLKIFHFARFDVVMMKRWMGITCAPIWCTKIASRLARTYTDRHGLKDVA
CCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHH
REVAGVDMSKAQQSSDWGQDKLTDAQIQYAASDVLYLHQIKAGLEAMLEREGRLELAQAC
HHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FDFLPIRADLDLAGWPDEDIFAHS
HHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNQITLHKGDLPEGLSLGPVIAVDTEAMGLNAMRDNLTLVQVSSGDGTAHLVQLTRDYDC
CCCEEEECCCCCCCCCCCCEEEECCHHHCCHHHHCCEEEEEEECCCCCEEEEEEECCCCC
PNLKALLTDPKVLKIFHFARFDVVMMKRWMGITCAPIWCTKIASRLARTYTDRHGLKDVA
CCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHH
REVAGVDMSKAQQSSDWGQDKLTDAQIQYAASDVLYLHQIKAGLEAMLEREGRLELAQAC
HHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FDFLPIRADLDLAGWPDEDIFAHS
HHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]