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The map label for this gene is mrcA [H]

Identifier: 114331785

GI number: 114331785

Start: 1918810

End: 1921122

Strand: Direct

Name: mrcA [H]

Synonym: Neut_1808

Alternate gene names: 114331785

Gene position: 1918810-1921122 (Clockwise)

Preceding gene: 114331747

Following gene: 114331790

Centisome position: 72.11

GC content: 48.12

Gene sequence:

>2313_bases
ATGTTATTCCGCTGGCTTTTCCGTTTCCTCCTAACTATTCTGGCTGCTGGTTTACTTGCGACGTTGCTCGTGATATTTGC
CGCCCTGGTTACGCTACCTTCTTTACCCAGTCTCGATATGCTGACTGATTATCGTCCGAAAATACCCTTGCGGATTTACA
GCGCTGATGGTTTGCTGATTGGGGAGTTTGGTGAAGAGCGCAGGGATTTTGTAAAAATAAATGAGGTACCAGAATCTCTT
AAACAAGCTGTGCTGGCAGCTGAAGACGATCGTTTTTACCAGCATTACGGGGTAGATTACATAGGTGTTTTGCGCGCCAT
TTATTCAAACTTCACGGCGGGCAGCACCCTCCAGGGCGCAAGCACCATTACCATGCAAGTGGCACGCAACTTCTTTCTGA
CCAAGGAAAAAACATTTACCAGAAAATTCAGCGAGGCATTACTGGCTTTTAAAATTGAACACAGCCTGGGAAAAGATCGC
ATTCTTGAGTTGTACATCAACCAGATTTACCTCGGGCAACGCAGCTATGGCTTTGCTACTGCAGCACAATCTTATTTTGG
TAAAAAACTGTCTGATATTAATTTGGCTGAAGCGGCCATGCTGGCCGGGCTCCCTAAAGCACCTTCACGCTACAATCCGA
TTGCCAACCTGGAACGCGCAAAAATACGACAACAATATGTGTTGCGGCGGATGTATGAGCTCAACTATATTTCAGCCGAC
CAACTCGCGCTAGCAAAGAAAACTTCTATTGCAATTCATCGCCAGGTAAAAGTATTTGCCATGCCGGCTGATTACGTCGC
AGAGATGGTGCGTCAGGTTGTTTACAATCGCTATCGTGAAGAAGCCTATACCCGTGGGATCAGGGTTTATACCACGCTGA
GAAAATCTGATCAGGAAGCAGCCTACCGTGCGCTGCGCAAAGGAGTAATAGAATATGACACCAGACATGGTTACCGCGGC
CCGGAAGCATTCCTGAAACTGCCAAAAGATCTCAAGGATCATGAAGCGCTGCAGGATATCATGCAAAATATCAGCAACAG
TGATGATATTTTGGCAGCCGTTGTCCTGTCCGTAACACCCGGTGCTGTTGAAGCCTCCCTCAAAAATGGGGAAACAATCC
GGATTACGGGAGATAGCCTGAAAGTAGCCAGCAGGTACCTTGGCAAAGATCCAAAGGTTACCGAAAAGCGAATACGCCCC
GGTGCTCTGATTCGAGTAAAAAAGACAGAAAAAAACAGCTGGCAAATTACTCAATTACCTGAAATAGAGGCCGCTCTGAT
CGCAATAGATCCAAACAATGGCGCGATTCGCGCATTAGTAGGCGGGTTTGACTATAACCTCAACAAATTCAATCATGCCA
CCCAGGCCTGGCGTCAACCCGGATCAAGTTTCAAACCGTTCATTTATTCCGCAGCACTTGAAAAAGGCTTTACTCCGGCC
ACCATTATTAATGACGCCCCACTGTATCTGGGACCTGATCAGACTGGTGGCAAGGCTTGGGAACCCAGGAATTATGACGG
CAAGTTCAGCGGCCCGATCAGAATGCGCACAGCATTAACTCATTCGAAAAACCTCGCATCCATCCGTATTCTTCAGGCGA
TTGGGCTATCCTACGCACAGGACTACATTACACGCTTCGGCTTCGATAAAAAACATCATCCAGCAGTTCTTCCCATGGCG
CTTGGCGCCGGATCGGTCACGCCGATGCAATTGGCCGTTGGCTACGCTACTTTCGCCAACGGGGGATACCATATTCCTCC
CTACTTCATTGAACGAATTGAGGATGAGCACGGAAAAATTATTGAGCAGACACGCCACACCACTGCAAGCCAGAACGCCA
AGCAGGTTATAGATCCACGCAATGCATTCCTGATGACAAACATGATGCAGGATGTTGTCCAAAGAGGAACTGCCGCCCGG
GCAAGAGCACTTGGCCGCAATGATATTGCCGGGAAAACGGGAACTACCAGCAACGCCTTCGATGCCTGGTTCTGCGGTTA
CCAGAAAGATCTGGTGGCGGTTACCTGGCTGGGCTTTGATGATCCCAAATCCATGGGAAACAGAGAAACCGGAGGGCAGG
CAGCCTTACCTATCTGGATCAACTATATGGCCAGCGCACTTAAATCAATACCTGTCACAAAAATATCTCCTCCAAAAGGT
ATTGTTACGGCCAAGATTAATTCAGAAACAGGCTTGAGGGATCCATTCGGGACGCTCAATGAATACTTCTTCCAGGAACA
GCTTCCGCCGGAAACAGATTATTCTTTCCAGGACCCTGGTTTTACGGATCAAATGGAAGACTGGCTGTTTTAA

Upstream 100 bases:

>100_bases
ACAGGTAAGTGAAAAAATCAATTCATGCAATAACAGTTGATATACCCTTTATAATCTTGTGCTGGATCATTTTTTTACCC
ACACAACACTCTGCCTTTTA

Downstream 100 bases:

>100_bases
ATCAGAGAGCAGTCGAGTGCAGGAGTATTTGCGCGGTTAACCCTCTTCTACCAGAGCTCCGTAAAGCCAATAAATTCAGC
AAGATAGATACAACGAACAA

Product: penicillin-binding protein, 1A family protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MLFRWLFRFLLTILAAGLLATLLVIFAALVTLPSLPSLDMLTDYRPKIPLRIYSADGLLIGEFGEERRDFVKINEVPESL
KQAVLAAEDDRFYQHYGVDYIGVLRAIYSNFTAGSTLQGASTITMQVARNFFLTKEKTFTRKFSEALLAFKIEHSLGKDR
ILELYINQIYLGQRSYGFATAAQSYFGKKLSDINLAEAAMLAGLPKAPSRYNPIANLERAKIRQQYVLRRMYELNYISAD
QLALAKKTSIAIHRQVKVFAMPADYVAEMVRQVVYNRYREEAYTRGIRVYTTLRKSDQEAAYRALRKGVIEYDTRHGYRG
PEAFLKLPKDLKDHEALQDIMQNISNSDDILAAVVLSVTPGAVEASLKNGETIRITGDSLKVASRYLGKDPKVTEKRIRP
GALIRVKKTEKNSWQITQLPEIEAALIAIDPNNGAIRALVGGFDYNLNKFNHATQAWRQPGSSFKPFIYSAALEKGFTPA
TIINDAPLYLGPDQTGGKAWEPRNYDGKFSGPIRMRTALTHSKNLASIRILQAIGLSYAQDYITRFGFDKKHHPAVLPMA
LGAGSVTPMQLAVGYATFANGGYHIPPYFIERIEDEHGKIIEQTRHTTASQNAKQVIDPRNAFLMTNMMQDVVQRGTAAR
ARALGRNDIAGKTGTTSNAFDAWFCGYQKDLVAVTWLGFDDPKSMGNRETGGQAALPIWINYMASALKSIPVTKISPPKG
IVTAKINSETGLRDPFGTLNEYFFQEQLPPETDYSFQDPGFTDQMEDWLF

Sequences:

>Translated_770_residues
MLFRWLFRFLLTILAAGLLATLLVIFAALVTLPSLPSLDMLTDYRPKIPLRIYSADGLLIGEFGEERRDFVKINEVPESL
KQAVLAAEDDRFYQHYGVDYIGVLRAIYSNFTAGSTLQGASTITMQVARNFFLTKEKTFTRKFSEALLAFKIEHSLGKDR
ILELYINQIYLGQRSYGFATAAQSYFGKKLSDINLAEAAMLAGLPKAPSRYNPIANLERAKIRQQYVLRRMYELNYISAD
QLALAKKTSIAIHRQVKVFAMPADYVAEMVRQVVYNRYREEAYTRGIRVYTTLRKSDQEAAYRALRKGVIEYDTRHGYRG
PEAFLKLPKDLKDHEALQDIMQNISNSDDILAAVVLSVTPGAVEASLKNGETIRITGDSLKVASRYLGKDPKVTEKRIRP
GALIRVKKTEKNSWQITQLPEIEAALIAIDPNNGAIRALVGGFDYNLNKFNHATQAWRQPGSSFKPFIYSAALEKGFTPA
TIINDAPLYLGPDQTGGKAWEPRNYDGKFSGPIRMRTALTHSKNLASIRILQAIGLSYAQDYITRFGFDKKHHPAVLPMA
LGAGSVTPMQLAVGYATFANGGYHIPPYFIERIEDEHGKIIEQTRHTTASQNAKQVIDPRNAFLMTNMMQDVVQRGTAAR
ARALGRNDIAGKTGTTSNAFDAWFCGYQKDLVAVTWLGFDDPKSMGNRETGGQAALPIWINYMASALKSIPVTKISPPKG
IVTAKINSETGLRDPFGTLNEYFFQEQLPPETDYSFQDPGFTDQMEDWLF
>Mature_770_residues
MLFRWLFRFLLTILAAGLLATLLVIFAALVTLPSLPSLDMLTDYRPKIPLRIYSADGLLIGEFGEERRDFVKINEVPESL
KQAVLAAEDDRFYQHYGVDYIGVLRAIYSNFTAGSTLQGASTITMQVARNFFLTKEKTFTRKFSEALLAFKIEHSLGKDR
ILELYINQIYLGQRSYGFATAAQSYFGKKLSDINLAEAAMLAGLPKAPSRYNPIANLERAKIRQQYVLRRMYELNYISAD
QLALAKKTSIAIHRQVKVFAMPADYVAEMVRQVVYNRYREEAYTRGIRVYTTLRKSDQEAAYRALRKGVIEYDTRHGYRG
PEAFLKLPKDLKDHEALQDIMQNISNSDDILAAVVLSVTPGAVEASLKNGETIRITGDSLKVASRYLGKDPKVTEKRIRP
GALIRVKKTEKNSWQITQLPEIEAALIAIDPNNGAIRALVGGFDYNLNKFNHATQAWRQPGSSFKPFIYSAALEKGFTPA
TIINDAPLYLGPDQTGGKAWEPRNYDGKFSGPIRMRTALTHSKNLASIRILQAIGLSYAQDYITRFGFDKKHHPAVLPMA
LGAGSVTPMQLAVGYATFANGGYHIPPYFIERIEDEHGKIIEQTRHTTASQNAKQVIDPRNAFLMTNMMQDVVQRGTAAR
ARALGRNDIAGKTGTTSNAFDAWFCGYQKDLVAVTWLGFDDPKSMGNRETGGQAALPIWINYMASALKSIPVTKISPPKG
IVTAKINSETGLRDPFGTLNEYFFQEQLPPETDYSFQDPGFTDQMEDWLF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=800, Percent_Identity=39, Blast_Score=570, Evalue=1e-163,
Organism=Escherichia coli, GI1786343, Length=315, Percent_Identity=32.6984126984127, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788867, Length=217, Percent_Identity=35.0230414746544, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1789601, Length=166, Percent_Identity=36.144578313253, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 86265; Mature: 86265

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFRWLFRFLLTILAAGLLATLLVIFAALVTLPSLPSLDMLTDYRPKIPLRIYSADGLLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECCCEEE
GEFGEERRDFVKINEVPESLKQAVLAAEDDRFYQHYGVDYIGVLRAIYSNFTAGSTLQGA
ECCCHHHHHHEEHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC
STITMQVARNFFLTKEKTFTRKFSEALLAFKIEHSLGKDRILELYINQIYLGQRSYGFAT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHH
AAQSYFGKKLSDINLAEAAMLAGLPKAPSRYNPIANLERAKIRQQYVLRRMYELNYISAD
HHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
QLALAKKTSIAIHRQVKVFAMPADYVAEMVRQVVYNRYREEAYTRGIRVYTTLRKSDQEA
HHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHCCCHHH
AYRALRKGVIEYDTRHGYRGPEAFLKLPKDLKDHEALQDIMQNISNSDDILAAVVLSVTP
HHHHHHCCHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
GAVEASLKNGETIRITGDSLKVASRYLGKDPKVTEKRIRPGALIRVKKTEKNSWQITQLP
CHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCHHHHCCCCEEEEEEECCCCCEEEEECC
EIEAALIAIDPNNGAIRALVGGFDYNLNKFNHATQAWRQPGSSFKPFIYSAALEKGFTPA
CCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCE
TIINDAPLYLGPDQTGGKAWEPRNYDGKFSGPIRMRTALTHSKNLASIRILQAIGLSYAQ
EEECCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHH
DYITRFGFDKKHHPAVLPMALGAGSVTPMQLAVGYATFANGGYHIPPYFIERIEDEHGKI
HHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHH
IEQTRHTTASQNAKQVIDPRNAFLMTNMMQDVVQRGTAARARALGRNDIAGKTGTTSNAF
HHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCC
DAWFCGYQKDLVAVTWLGFDDPKSMGNRETGGQAALPIWINYMASALKSIPVTKISPPKG
HHHHCCCCCCEEEEEEECCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCC
IVTAKINSETGLRDPFGTLNEYFFQEQLPPETDYSFQDPGFTDQMEDWLF
EEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MLFRWLFRFLLTILAAGLLATLLVIFAALVTLPSLPSLDMLTDYRPKIPLRIYSADGLLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECCCEEE
GEFGEERRDFVKINEVPESLKQAVLAAEDDRFYQHYGVDYIGVLRAIYSNFTAGSTLQGA
ECCCHHHHHHEEHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC
STITMQVARNFFLTKEKTFTRKFSEALLAFKIEHSLGKDRILELYINQIYLGQRSYGFAT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHH
AAQSYFGKKLSDINLAEAAMLAGLPKAPSRYNPIANLERAKIRQQYVLRRMYELNYISAD
HHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
QLALAKKTSIAIHRQVKVFAMPADYVAEMVRQVVYNRYREEAYTRGIRVYTTLRKSDQEA
HHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHCCCHHH
AYRALRKGVIEYDTRHGYRGPEAFLKLPKDLKDHEALQDIMQNISNSDDILAAVVLSVTP
HHHHHHCCHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
GAVEASLKNGETIRITGDSLKVASRYLGKDPKVTEKRIRPGALIRVKKTEKNSWQITQLP
CHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCHHHHCCCCEEEEEEECCCCCEEEEECC
EIEAALIAIDPNNGAIRALVGGFDYNLNKFNHATQAWRQPGSSFKPFIYSAALEKGFTPA
CCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCE
TIINDAPLYLGPDQTGGKAWEPRNYDGKFSGPIRMRTALTHSKNLASIRILQAIGLSYAQ
EEECCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHH
DYITRFGFDKKHHPAVLPMALGAGSVTPMQLAVGYATFANGGYHIPPYFIERIEDEHGKI
HHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHH
IEQTRHTTASQNAKQVIDPRNAFLMTNMMQDVVQRGTAARARALGRNDIAGKTGTTSNAF
HHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCC
DAWFCGYQKDLVAVTWLGFDDPKSMGNRETGGQAALPIWINYMASALKSIPVTKISPPKG
HHHHCCCCCCEEEEEEECCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCC
IVTAKINSETGLRDPFGTLNEYFFQEQLPPETDYSFQDPGFTDQMEDWLF
EEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA