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The map label for this gene is gatB

Identifier: 114331582

GI number: 114331582

Start: 1676378

End: 1677814

Strand: Direct

Name: gatB

Synonym: Neut_1595

Alternate gene names: 114331582

Gene position: 1676378-1677814 (Clockwise)

Preceding gene: 114331581

Following gene: 114331585

Centisome position: 63.0

GC content: 46.9

Gene sequence:

>1437_bases
ATGCAATGGGAAACTGTCATCGGCCTTGAGATACATGTGCAGCTATTGACCCGATCCAAAATTTTTTCTGGTGCATCCGT
TGCGTATGGCGCCCGGCCGAATTCACAAGCCTGCGCAGTGGATATTGCCCTGCCCGGGGTATTGCCAGTATTAAACCGGC
AAGTGGTTGAATATGCTATCCGCTTTGGTCTGGCAATTAATGCAACCATTAACTCACCCTCAATCTTTGCCCGAAAAAAC
TATTTTTATCCTGATTTACCCAAAGGCTACCAAATCAGTCAGCTGGCACATCCAATCGTTGAAGGTGGGCATGTTACCAT
CCGGACAGGGGATAAAGAAAAAACCATACACCTGACACGCGCCCATCTGGAAGAAGACGCAGGTAAATCAACACACGAAG
GTTTTCACGACAAGACAGGAATCGACCTGAATCGTGCCGGAACCGGCTTACTAGAAATTGTCTCGGAACCAGAGATGCGC
AGCAGTGCCGAGGCTGTGGCCTACGCAAGAGTACTTCATTCACTGGTGCGCTGGATCGGTATTTGTGACGGCAACATGCA
GGAAGGATCCTTCCGTTGCGATGCCAATGTTTCAGTACGACCTGCTGGCTCGGACGTACTGGGGACACGCTGTGAAATCA
AAAACCTCAATTCATTCCGTTTTCTTGAAAGAGCAATCGATTATGAAGTGGCAAGACAAATTGATATTCTGGAAAGTGGC
GGCAGCATAGTCCAGCAAACACGACTATATGATGCAGACCGGGATGAAACCCGCACCATGCGCAGCAAGGAAGATGCACA
TGACTACCGTTATTTCCCGGATCCCGACCTGTTGCCAGTTGTTATCCCCCAGGAATGGATAAAACAGATCAGAGAGAAAT
TGCCGGAATTACCGGAAGATAGACACAATCGCTATATTCGGGACTTCAGTCTATCCGCCTACGATGCCAATATACTAACC
GCGGCACGTGAACTGGCTGACTATTTTGAGCAAACCGTCAAATTTCTCCCGGAACAAATTAAACTTTGTGCTAACTGGAT
TATAGGAGAAGTCAGTGGAAGACTGAACAAGGAAGGACTCGAAATTGACCAGTGTCCCGTCAGTCCTGAACAACTGGCAG
GATTGCTGCTACGGATCACGGATGGCACCGTATCAGGAAAAATAGCCAAGGATGTATTTGATAGTATGTGGCAAAGCAAC
GATAACAGTGCCGATACAATTATCGATGCAAAAAACCTCAGGCAGATTTCAGATAACAGCGAAATTGAGAAATGGGTTAA
TGAGGTGCTTGCAGCAAATCAACAACAAGTAGCCGATTATCGCGCTGGAAAAGAAAAAGCGTTTAATTCCCTGGTCGGCC
AGGTAATGAAAATGTCAAGAGGCAAGGCCAATCCCGTCCAGGTCAATACACTGCTTAAAAAACACTTGATAGAATAA

Upstream 100 bases:

>100_bases
TGGCATGAGCTTACTCCACCGGAAATGAATCGCTAAAAATCTTCAGGCCTCTGCTCTCTGGTTAATATCAAGCAACTAAA
AGAAAAAGGATTACCTGATC

Downstream 100 bases:

>100_bases
AACCCTGGATGAATTCAGCATACCGCTCAAAACAAAAAATTCAGGTCCGATAGCTGCTCCAGATCGAGCCTGAATTTCAT
GCATAAACTCTGTTTTACTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MQWETVIGLEIHVQLLTRSKIFSGASVAYGARPNSQACAVDIALPGVLPVLNRQVVEYAIRFGLAINATINSPSIFARKN
YFYPDLPKGYQISQLAHPIVEGGHVTIRTGDKEKTIHLTRAHLEEDAGKSTHEGFHDKTGIDLNRAGTGLLEIVSEPEMR
SSAEAVAYARVLHSLVRWIGICDGNMQEGSFRCDANVSVRPAGSDVLGTRCEIKNLNSFRFLERAIDYEVARQIDILESG
GSIVQQTRLYDADRDETRTMRSKEDAHDYRYFPDPDLLPVVIPQEWIKQIREKLPELPEDRHNRYIRDFSLSAYDANILT
AARELADYFEQTVKFLPEQIKLCANWIIGEVSGRLNKEGLEIDQCPVSPEQLAGLLLRITDGTVSGKIAKDVFDSMWQSN
DNSADTIIDAKNLRQISDNSEIEKWVNEVLAANQQQVADYRAGKEKAFNSLVGQVMKMSRGKANPVQVNTLLKKHLIE

Sequences:

>Translated_478_residues
MQWETVIGLEIHVQLLTRSKIFSGASVAYGARPNSQACAVDIALPGVLPVLNRQVVEYAIRFGLAINATINSPSIFARKN
YFYPDLPKGYQISQLAHPIVEGGHVTIRTGDKEKTIHLTRAHLEEDAGKSTHEGFHDKTGIDLNRAGTGLLEIVSEPEMR
SSAEAVAYARVLHSLVRWIGICDGNMQEGSFRCDANVSVRPAGSDVLGTRCEIKNLNSFRFLERAIDYEVARQIDILESG
GSIVQQTRLYDADRDETRTMRSKEDAHDYRYFPDPDLLPVVIPQEWIKQIREKLPELPEDRHNRYIRDFSLSAYDANILT
AARELADYFEQTVKFLPEQIKLCANWIIGEVSGRLNKEGLEIDQCPVSPEQLAGLLLRITDGTVSGKIAKDVFDSMWQSN
DNSADTIIDAKNLRQISDNSEIEKWVNEVLAANQQQVADYRAGKEKAFNSLVGQVMKMSRGKANPVQVNTLLKKHLIE
>Mature_478_residues
MQWETVIGLEIHVQLLTRSKIFSGASVAYGARPNSQACAVDIALPGVLPVLNRQVVEYAIRFGLAINATINSPSIFARKN
YFYPDLPKGYQISQLAHPIVEGGHVTIRTGDKEKTIHLTRAHLEEDAGKSTHEGFHDKTGIDLNRAGTGLLEIVSEPEMR
SSAEAVAYARVLHSLVRWIGICDGNMQEGSFRCDANVSVRPAGSDVLGTRCEIKNLNSFRFLERAIDYEVARQIDILESG
GSIVQQTRLYDADRDETRTMRSKEDAHDYRYFPDPDLLPVVIPQEWIKQIREKLPELPEDRHNRYIRDFSLSAYDANILT
AARELADYFEQTVKFLPEQIKLCANWIIGEVSGRLNKEGLEIDQCPVSPEQLAGLLLRITDGTVSGKIAKDVFDSMWQSN
DNSADTIIDAKNLRQISDNSEIEKWVNEVLAANQQQVADYRAGKEKAFNSLVGQVMKMSRGKANPVQVNTLLKKHLIE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=39.3939393939394, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=476, Percent_Identity=31.0924369747899, Blast_Score=221, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=513, Percent_Identity=31.5789473684211, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI45550808, Length=493, Percent_Identity=35.2941176470588, Blast_Score=308, Evalue=5e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_NITEC (Q0AFP3)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747804.1
- ProteinModelPortal:   Q0AFP3
- SMR:   Q0AFP3
- STRING:   Q0AFP3
- GeneID:   4272353
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1595
- NMPDR:   fig|335283.3.peg.1899
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q0AFP3
- ProtClustDB:   PRK05477
- BioCyc:   NEUT335283:NEUT_1595-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53591; Mature: 53591

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWETVIGLEIHVQLLTRSKIFSGASVAYGARPNSQACAVDIALPGVLPVLNRQVVEYAI
CCCCEEEEHEEEEEEHHHHHHCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
RFGLAINATINSPSIFARKNYFYPDLPKGYQISQLAHPIVEGGHVTIRTGDKEKTIHLTR
HHCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEHHH
AHLEEDAGKSTHEGFHDKTGIDLNRAGTGLLEIVSEPEMRSSAEAVAYARVLHSLVRWIG
HHHHHHCCCCHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ICDGNMQEGSFRCDANVSVRPAGSDVLGTRCEIKNLNSFRFLERAIDYEVARQIDILESG
CCCCCCCCCCEEECCCCEEECCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GSIVQQTRLYDADRDETRTMRSKEDAHDYRYFPDPDLLPVVIPQEWIKQIREKLPELPED
CHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCH
RHNRYIRDFSLSAYDANILTAARELADYFEQTVKFLPEQIKLCANWIIGEVSGRLNKEGL
HHCCEEHHCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EIDQCPVSPEQLAGLLLRITDGTVSGKIAKDVFDSMWQSNDNSADTIIDAKNLRQISDNS
CCCCCCCCHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHEEEHHHHHHHCCCH
EIEKWVNEVLAANQQQVADYRAGKEKAFNSLVGQVMKMSRGKANPVQVNTLLKKHLIE
HHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQWETVIGLEIHVQLLTRSKIFSGASVAYGARPNSQACAVDIALPGVLPVLNRQVVEYAI
CCCCEEEEHEEEEEEHHHHHHCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
RFGLAINATINSPSIFARKNYFYPDLPKGYQISQLAHPIVEGGHVTIRTGDKEKTIHLTR
HHCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEHHH
AHLEEDAGKSTHEGFHDKTGIDLNRAGTGLLEIVSEPEMRSSAEAVAYARVLHSLVRWIG
HHHHHHCCCCHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ICDGNMQEGSFRCDANVSVRPAGSDVLGTRCEIKNLNSFRFLERAIDYEVARQIDILESG
CCCCCCCCCCEEECCCCEEECCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GSIVQQTRLYDADRDETRTMRSKEDAHDYRYFPDPDLLPVVIPQEWIKQIREKLPELPED
CHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCH
RHNRYIRDFSLSAYDANILTAARELADYFEQTVKFLPEQIKLCANWIIGEVSGRLNKEGL
HHCCEEHHCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EIDQCPVSPEQLAGLLLRITDGTVSGKIAKDVFDSMWQSNDNSADTIIDAKNLRQISDNS
CCCCCCCCHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHEEEHHHHHHHCCCH
EIEKWVNEVLAANQQQVADYRAGKEKAFNSLVGQVMKMSRGKANPVQVNTLLKKHLIE
HHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA