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The map label for this gene is gatA

Identifier: 114331581

GI number: 114331581

Start: 1674853

End: 1676313

Strand: Direct

Name: gatA

Synonym: Neut_1594

Alternate gene names: 114331581

Gene position: 1674853-1676313 (Clockwise)

Preceding gene: 162219160

Following gene: 114331582

Centisome position: 62.94

GC content: 49.97

Gene sequence:

>1461_bases
ATGCTAAATGCCAGTCTCAGGCAGCTCTCTTCGCTGCTCTCAGAAAAAAAAATATCCAGCACAGAACTCACCGGGGAATT
TCTAGCCCGGATCAAGGCCCTCAATCCTGACTTGAATGCCTTTATTACAATGGATGAAGAAAAAAGTCTGGATCAGGCCA
GAACCGCTGACGAGATGATTGCCACGGGACAATCCACACTGCTCACAGGAATACCCATCGCCCAAAAAGATATTTTCTGT
GCCAACGGGTGGCTGACAACTTGCGGATCGAAAATGCTTTCAAATTTTATTTCACCCTATGATGCCACCGTGGTGAAACA
GTTTGATCAGGTGGGGATGGTCAATCTGGGCAAAACCAACATGGATGAATTCGCCATGGGCTCCAGTAACGAAACTTCCT
ATTACGGGCCAGTGAAAAATCCCTGGGACAGGCTCGCCGTTCCTGGCGGCAGCTCAGGCGGCTCGGCCTGTGCGGTAGCA
GCCAGACTGGCTCCAGCAGCAACGGGCACCGATACAGGTGGCTCCATTCGTCAACCAGCTGCATTATGTGGAATTAGTGG
AATCAAACCTACTTACGGACTGGTTTCTCGGTACGGTATGATCGCATTTGCTTCCAGCCTCGATCAGGCTGGACCAATGG
CCAGATCTGCCGAAGATCTTGCACTGCTGCTGAATGTCATGGTCGGATTTGATGAACGTGATTCAACCAGTCTACAGCGG
GAAAAAGAAGACTATACGCAAGACCTGGAGAAACCGGTGAGCGGGCTGCGCATTGGCTTACCCAAGGAATTTTTTGCAGA
AGGTATGAGCAGTGACGTTTCCAGTGCGGTAGAAGCGGCTCTGGCAGAGTACCGCAAACTGGGCGCCACATTTGTCGAGG
TATCCCTGCCAAACAGCAAGCTTGCCGTTCCAGTCTATTACGTGCTCGCACCCGCCGAAGCATCTAGTAACTTATCGCGT
TTTGATGGAGTACGATATGGCCATCGTGCCGCGCAATATGCCAATCTGGAAGATTTATACGCAAAAACACGCGCAGAAGG
ATTTGGCGATGAAGTAAAGCGCCGTATTCTTATTGGCACGTACGTGTTATCCCATGGCTATTACGATGCCTACTATCTTC
AGGCTCAAAAATTACGCCGTCTGATTGCCGAGGATTTCAAAAAAGCTTTTGAACAATGCGACCTGATCATGGGGCCGACT
TCACCTACCGTAGCCTTTAATATTGGCGAGAGATGCAACGATCCTATCCAGATGTATCTCTCCGATATCTACACCAGCGC
AGCCAGCCTTGCAGGATTGCCGGGTATGTCGATACCTGTTGGATTTGGCAGTAAAAATAGGCCGGTAGGCTTACATATTA
TTGGGAACTACTTCAGGGAAGCACAAATGCTGAATGCGGCTCACCGCTACCAGCTGGCGACAAACTGGCATGAGCTTACT
CCACCGGAAATGAATCGCTAA

Upstream 100 bases:

>100_bases
GATGGATACTATCTGGTTCCCAAGGTAATCGAATAACACCGGCAATAAAACCGTATCTCTGCTTATCAATTTTCCAAATA
TTCCACCGCACTGACAGATC

Downstream 100 bases:

>100_bases
AAATCTTCAGGCCTCTGCTCTCTGGTTAATATCAAGCAACTAAAAGAAAAAGGATTACCTGATCATGCAATGGGAAACTG
TCATCGGCCTTGAGATACAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MLNASLRQLSSLLSEKKISSTELTGEFLARIKALNPDLNAFITMDEEKSLDQARTADEMIATGQSTLLTGIPIAQKDIFC
ANGWLTTCGSKMLSNFISPYDATVVKQFDQVGMVNLGKTNMDEFAMGSSNETSYYGPVKNPWDRLAVPGGSSGGSACAVA
ARLAPAATGTDTGGSIRQPAALCGISGIKPTYGLVSRYGMIAFASSLDQAGPMARSAEDLALLLNVMVGFDERDSTSLQR
EKEDYTQDLEKPVSGLRIGLPKEFFAEGMSSDVSSAVEAALAEYRKLGATFVEVSLPNSKLAVPVYYVLAPAEASSNLSR
FDGVRYGHRAAQYANLEDLYAKTRAEGFGDEVKRRILIGTYVLSHGYYDAYYLQAQKLRRLIAEDFKKAFEQCDLIMGPT
SPTVAFNIGERCNDPIQMYLSDIYTSAASLAGLPGMSIPVGFGSKNRPVGLHIIGNYFREAQMLNAAHRYQLATNWHELT
PPEMNR

Sequences:

>Translated_486_residues
MLNASLRQLSSLLSEKKISSTELTGEFLARIKALNPDLNAFITMDEEKSLDQARTADEMIATGQSTLLTGIPIAQKDIFC
ANGWLTTCGSKMLSNFISPYDATVVKQFDQVGMVNLGKTNMDEFAMGSSNETSYYGPVKNPWDRLAVPGGSSGGSACAVA
ARLAPAATGTDTGGSIRQPAALCGISGIKPTYGLVSRYGMIAFASSLDQAGPMARSAEDLALLLNVMVGFDERDSTSLQR
EKEDYTQDLEKPVSGLRIGLPKEFFAEGMSSDVSSAVEAALAEYRKLGATFVEVSLPNSKLAVPVYYVLAPAEASSNLSR
FDGVRYGHRAAQYANLEDLYAKTRAEGFGDEVKRRILIGTYVLSHGYYDAYYLQAQKLRRLIAEDFKKAFEQCDLIMGPT
SPTVAFNIGERCNDPIQMYLSDIYTSAASLAGLPGMSIPVGFGSKNRPVGLHIIGNYFREAQMLNAAHRYQLATNWHELT
PPEMNR
>Mature_486_residues
MLNASLRQLSSLLSEKKISSTELTGEFLARIKALNPDLNAFITMDEEKSLDQARTADEMIATGQSTLLTGIPIAQKDIFC
ANGWLTTCGSKMLSNFISPYDATVVKQFDQVGMVNLGKTNMDEFAMGSSNETSYYGPVKNPWDRLAVPGGSSGGSACAVA
ARLAPAATGTDTGGSIRQPAALCGISGIKPTYGLVSRYGMIAFASSLDQAGPMARSAEDLALLLNVMVGFDERDSTSLQR
EKEDYTQDLEKPVSGLRIGLPKEFFAEGMSSDVSSAVEAALAEYRKLGATFVEVSLPNSKLAVPVYYVLAPAEASSNLSR
FDGVRYGHRAAQYANLEDLYAKTRAEGFGDEVKRRILIGTYVLSHGYYDAYYLQAQKLRRLIAEDFKKAFEQCDLIMGPT
SPTVAFNIGERCNDPIQMYLSDIYTSAASLAGLPGMSIPVGFGSKNRPVGLHIIGNYFREAQMLNAAHRYQLATNWHELT
PPEMNR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=39.9602385685885, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=26.6260162601626, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI166795287, Length=214, Percent_Identity=29.9065420560748, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=436, Percent_Identity=37.1559633027523, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=25, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=297, Percent_Identity=28.6195286195286, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=316, Percent_Identity=27.8481012658228, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=316, Percent_Identity=27.8481012658228, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=243, Percent_Identity=28.3950617283951, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=425, Percent_Identity=37.8823529411765, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319685, Length=475, Percent_Identity=26.7368421052632, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=40.8163265306122, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=26.5346534653465, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=26.5346534653465, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=26.5346534653465, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=26.5346534653465, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=25.4901960784314, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078093, Length=503, Percent_Identity=26.8389662027833, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45550774, Length=468, Percent_Identity=25.4273504273504, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648435, Length=468, Percent_Identity=25.4273504273504, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648437, Length=468, Percent_Identity=25.4273504273504, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356731, Length=485, Percent_Identity=25.979381443299, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648441, Length=403, Percent_Identity=24.8138957816377, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=403, Percent_Identity=24.8138957816377, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_NITEC (Q0AFP4)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747803.1
- ProteinModelPortal:   Q0AFP4
- SMR:   Q0AFP4
- STRING:   Q0AFP4
- GeneID:   4272352
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1594
- NMPDR:   fig|335283.3.peg.1898
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q0AFP4
- ProtClustDB:   PRK00012
- BioCyc:   NEUT335283:NEUT_1594-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52772; Mature: 52772

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 151-151 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNASLRQLSSLLSEKKISSTELTGEFLARIKALNPDLNAFITMDEEKSLDQARTADEMI
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
ATGQSTLLTGIPIAQKDIFCANGWLTTCGSKMLSNFISPYDATVVKQFDQVGMVNLGKTN
HCCCHHHEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEECCCCC
MDEFAMGSSNETSYYGPVKNPWDRLAVPGGSSGGSACAVAARLAPAATGTDTGGSIRQPA
HHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCH
ALCGISGIKPTYGLVSRYGMIAFASSLDQAGPMARSAEDLALLLNVMVGFDERDSTSLQR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
EKEDYTQDLEKPVSGLRIGLPKEFFAEGMSSDVSSAVEAALAEYRKLGATFVEVSLPNSK
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
LAVPVYYVLAPAEASSNLSRFDGVRYGHRAAQYANLEDLYAKTRAEGFGDEVKRRILIGT
EEEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHH
YVLSHGYYDAYYLQAQKLRRLIAEDFKKAFEQCDLIMGPTSPTVAFNIGERCNDPIQMYL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEECCHHCCHHHHHHH
SDIYTSAASLAGLPGMSIPVGFGSKNRPVGLHIIGNYFREAQMLNAAHRYQLATNWHELT
HHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPEMNR
CCCCCC
>Mature Secondary Structure
MLNASLRQLSSLLSEKKISSTELTGEFLARIKALNPDLNAFITMDEEKSLDQARTADEMI
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
ATGQSTLLTGIPIAQKDIFCANGWLTTCGSKMLSNFISPYDATVVKQFDQVGMVNLGKTN
HCCCHHHEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEECCCCC
MDEFAMGSSNETSYYGPVKNPWDRLAVPGGSSGGSACAVAARLAPAATGTDTGGSIRQPA
HHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCH
ALCGISGIKPTYGLVSRYGMIAFASSLDQAGPMARSAEDLALLLNVMVGFDERDSTSLQR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
EKEDYTQDLEKPVSGLRIGLPKEFFAEGMSSDVSSAVEAALAEYRKLGATFVEVSLPNSK
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
LAVPVYYVLAPAEASSNLSRFDGVRYGHRAAQYANLEDLYAKTRAEGFGDEVKRRILIGT
EEEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHH
YVLSHGYYDAYYLQAQKLRRLIAEDFKKAFEQCDLIMGPTSPTVAFNIGERCNDPIQMYL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEECCHHCCHHHHHHH
SDIYTSAASLAGLPGMSIPVGFGSKNRPVGLHIIGNYFREAQMLNAAHRYQLATNWHELT
HHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPEMNR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA