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The map label for this gene is pykA [H]

Identifier: 114331567

GI number: 114331567

Start: 1659904

End: 1661394

Strand: Direct

Name: pykA [H]

Synonym: Neut_1580

Alternate gene names: 114331567

Gene position: 1659904-1661394 (Clockwise)

Preceding gene: 114331566

Following gene: 114331568

Centisome position: 62.38

GC content: 48.76

Gene sequence:

>1491_bases
ATGATGCGAAGAACAAAAATTGTAGCAACGCTGGGACCAGCTTCCAGCAACCCTGATATCCTGAGACAAATGATTGAGGC
CGGTGTCGATGTCGTCCGTATCAATTTTTCACATGGAACAAAGGACGAGCATATTGCTTCCGCGGAGCAAGTACGCTCAC
TTGCCCATTCACTTGGCCGTACGGTAGGTGTGCTGGCCGATCTGCAAGGACCAAAAATTCGTATCGGCAAGTTTGCTCAG
GGAAAGATCACGCTCAAAGCCAGGGATGAATTTATTCTTGATGCAGAGTGCGAGCTGGGTAACCAGGAACGGGTCGGGCT
GGATTACCGGGAGCTGCCCAACGATGTTGAAGCAGGTACTGTCCTCTTGCTGGATGACGGCCGAATCGTTCTGACTGTGA
CCAAAGTCAGGGAAAGTGAAATCTATTGCGAAGTTTTACAAGGCGGGATACTTTCCAATAATAAAGGCATAAACCGGAAA
GGTGGAGGTCTGAGTGCCCCCGCTCTGACAGCAAAGGATCTGCAGGATATCAAAACTGCCACAATTTTACGGGCAGATTA
TCTTGCGGTGTCATTTCCTCGCTCGGGAGAAGATATCAGGCGAGCGCGTGCATTAATGCAGGAGGCTCAGGGGCATAGTT
TGCTCATGGCAAAAATTGAGCGCTCGGAAGCTATTCTGGCGTTGGATGATATTCTAAGTGCTTCCGATGCAATTATGGTC
GCGCGGGGCGATCTGGCAGTTGAAGTAGGTGATGCAGCAGTTCCTGCCTTGCAAAAACGCATGATACGATCAGCACGTGA
AGCCAATAAATTGGTGATTACAGCCACGCAAATGATGGAATCGATGATATCCAATCCCATCCCAACACGTGCAGAGGTAT
CAGACGTTGCAAATGCAGTGCTGGATGGTACGGATGCTGTCATGCTGTCTGCTGAATCAGCTGCAGGACAATACCCGGTA
GAAGCCGTAGCAGCCATGGCAAGAGTTTGTCTGGAGGCTGAAAAGGAGTATACGGTTAATCTGAGTTTTCGCCGTTCTCC
GGATACACAACCAGTCAGTATCGAAGACGCTATTGCGCGAGCTACCATGTACACCGCCGGTTCGCTCAAGATTCGAGCAA
TCGCTGCATTGACACAAAGCGGCGTAACAGCATTGTTCATGTCTCGTAGAAGCTCGAAAGTTCCAATCTTCGCTTTAAGT
CCACAAGAGGATACTCTGAGCAAGGTCACTCTTTTTCGTGGTGTTTATCCTATAAAATTTGGACAGGGATTGTGTGATCC
TGAGATTATCCTCAGCATGGCGGAAGATGAGTTGCTCAGACGCGGCGCGGTACGAAACGAAGATTTAATCATCATGACAA
TTGGCGAACCGGTTGGCAAGGCTGGCGGAACAAATACTATGAAACTTGTCAGAGTAGGTGGTTCCAGGGGAACGACACAG
ATAACGAAAGAAGTGACTCAGCAGACCTTTTCTGATCCACTTAATTTATAA

Upstream 100 bases:

>100_bases
TTCCTGGAATTCTTAGAAGGAAAAAAACTACCCGCTGTCGAGATCCTGGAATTACGTGCACAGTGAGGCCTGAGAAGATT
TTCTTCCAGGAATTTCGGAA

Downstream 100 bases:

>100_bases
AATTAACCCTACAAACAGATTAGCGTATCGGCTTATACACCCGTAAGAACATGAAGTTTGGTTTCATCAGAAAATAAACA
TATTAATTTTATATAAAGGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MMRRTKIVATLGPASSNPDILRQMIEAGVDVVRINFSHGTKDEHIASAEQVRSLAHSLGRTVGVLADLQGPKIRIGKFAQ
GKITLKARDEFILDAECELGNQERVGLDYRELPNDVEAGTVLLLDDGRIVLTVTKVRESEIYCEVLQGGILSNNKGINRK
GGGLSAPALTAKDLQDIKTATILRADYLAVSFPRSGEDIRRARALMQEAQGHSLLMAKIERSEAILALDDILSASDAIMV
ARGDLAVEVGDAAVPALQKRMIRSAREANKLVITATQMMESMISNPIPTRAEVSDVANAVLDGTDAVMLSAESAAGQYPV
EAVAAMARVCLEAEKEYTVNLSFRRSPDTQPVSIEDAIARATMYTAGSLKIRAIAALTQSGVTALFMSRRSSKVPIFALS
PQEDTLSKVTLFRGVYPIKFGQGLCDPEIILSMAEDELLRRGAVRNEDLIIMTIGEPVGKAGGTNTMKLVRVGGSRGTTQ
ITKEVTQQTFSDPLNL

Sequences:

>Translated_496_residues
MMRRTKIVATLGPASSNPDILRQMIEAGVDVVRINFSHGTKDEHIASAEQVRSLAHSLGRTVGVLADLQGPKIRIGKFAQ
GKITLKARDEFILDAECELGNQERVGLDYRELPNDVEAGTVLLLDDGRIVLTVTKVRESEIYCEVLQGGILSNNKGINRK
GGGLSAPALTAKDLQDIKTATILRADYLAVSFPRSGEDIRRARALMQEAQGHSLLMAKIERSEAILALDDILSASDAIMV
ARGDLAVEVGDAAVPALQKRMIRSAREANKLVITATQMMESMISNPIPTRAEVSDVANAVLDGTDAVMLSAESAAGQYPV
EAVAAMARVCLEAEKEYTVNLSFRRSPDTQPVSIEDAIARATMYTAGSLKIRAIAALTQSGVTALFMSRRSSKVPIFALS
PQEDTLSKVTLFRGVYPIKFGQGLCDPEIILSMAEDELLRRGAVRNEDLIIMTIGEPVGKAGGTNTMKLVRVGGSRGTTQ
ITKEVTQQTFSDPLNL
>Mature_496_residues
MMRRTKIVATLGPASSNPDILRQMIEAGVDVVRINFSHGTKDEHIASAEQVRSLAHSLGRTVGVLADLQGPKIRIGKFAQ
GKITLKARDEFILDAECELGNQERVGLDYRELPNDVEAGTVLLLDDGRIVLTVTKVRESEIYCEVLQGGILSNNKGINRK
GGGLSAPALTAKDLQDIKTATILRADYLAVSFPRSGEDIRRARALMQEAQGHSLLMAKIERSEAILALDDILSASDAIMV
ARGDLAVEVGDAAVPALQKRMIRSAREANKLVITATQMMESMISNPIPTRAEVSDVANAVLDGTDAVMLSAESAAGQYPV
EAVAAMARVCLEAEKEYTVNLSFRRSPDTQPVSIEDAIARATMYTAGSLKIRAIAALTQSGVTALFMSRRSSKVPIFALS
PQEDTLSKVTLFRGVYPIKFGQGLCDPEIILSMAEDELLRRGAVRNEDLIIMTIGEPVGKAGGTNTMKLVRVGGSRGTTQ
ITKEVTQQTFSDPLNL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=502, Percent_Identity=37.4501992031873, Blast_Score=278, Evalue=9e-75,
Organism=Homo sapiens, GI33286422, Length=501, Percent_Identity=36.3273453093812, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI33286420, Length=501, Percent_Identity=36.3273453093812, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=34.6855983772819, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=34.6855983772819, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI310128732, Length=294, Percent_Identity=36.3945578231293, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI310128730, Length=294, Percent_Identity=36.3945578231293, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI310128736, Length=242, Percent_Identity=33.4710743801653, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI310128734, Length=242, Percent_Identity=33.4710743801653, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI310128738, Length=221, Percent_Identity=32.579185520362, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=54.0880503144654, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1787965, Length=468, Percent_Identity=38.2478632478632, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=36.25, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI71984406, Length=490, Percent_Identity=35.1020408163265, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=35.2577319587629, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71984413, Length=490, Percent_Identity=35.1020408163265, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17506829, Length=490, Percent_Identity=35.1020408163265, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=32.5102880658436, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=33.4012219959267, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=35.6275303643725, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=35.6275303643725, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24648966, Length=404, Percent_Identity=37.6237623762376, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24581235, Length=433, Percent_Identity=33.0254041570439, Blast_Score=221, Evalue=8e-58,
Organism=Drosophila melanogaster, GI24646914, Length=271, Percent_Identity=33.9483394833948, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53444; Mature: 53444

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRRTKIVATLGPASSNPDILRQMIEAGVDVVRINFSHGTKDEHIASAEQVRSLAHSLGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
TVGVLADLQGPKIRIGKFAQGKITLKARDEFILDAECELGNQERVGLDYRELPNDVEAGT
HHHHHCCCCCCEEEECEEECCEEEEEECCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCE
VLLLDDGRIVLTVTKVRESEIYCEVLQGGILSNNKGINRKGGGLSAPALTAKDLQDIKTA
EEEEECCEEEEEEEECCCHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TILRADYLAVSFPRSGEDIRRARALMQEAQGHSLLMAKIERSEAILALDDILSASDAIMV
HHEEECEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEHHHHHCCCCEEEE
ARGDLAVEVGDAAVPALQKRMIRSAREANKLVITATQMMESMISNPIPTRAEVSDVANAV
EECCEEEEECCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHH
LDGTDAVMLSAESAAGQYPVEAVAAMARVCLEAEKEYTVNLSFRRSPDTQPVSIEDAIAR
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHH
ATMYTAGSLKIRAIAALTQSGVTALFMSRRSSKVPIFALSPQEDTLSKVTLFRGVYPIKF
HHHEECCCEEEEEEEHHHHCCHHEEHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCEEEC
GQGLCDPEIILSMAEDELLRRGAVRNEDLIIMTIGEPVGKAGGTNTMKLVRVGGSRGTTQ
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCHH
ITKEVTQQTFSDPLNL
HHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMRRTKIVATLGPASSNPDILRQMIEAGVDVVRINFSHGTKDEHIASAEQVRSLAHSLGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
TVGVLADLQGPKIRIGKFAQGKITLKARDEFILDAECELGNQERVGLDYRELPNDVEAGT
HHHHHCCCCCCEEEECEEECCEEEEEECCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCE
VLLLDDGRIVLTVTKVRESEIYCEVLQGGILSNNKGINRKGGGLSAPALTAKDLQDIKTA
EEEEECCEEEEEEEECCCHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TILRADYLAVSFPRSGEDIRRARALMQEAQGHSLLMAKIERSEAILALDDILSASDAIMV
HHEEECEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEHHHHHCCCCEEEE
ARGDLAVEVGDAAVPALQKRMIRSAREANKLVITATQMMESMISNPIPTRAEVSDVANAV
EECCEEEEECCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHH
LDGTDAVMLSAESAAGQYPVEAVAAMARVCLEAEKEYTVNLSFRRSPDTQPVSIEDAIAR
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHH
ATMYTAGSLKIRAIAALTQSGVTALFMSRRSSKVPIFALSPQEDTLSKVTLFRGVYPIKF
HHHEECCCEEEEEEEHHHHCCHHEEHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCEEEC
GQGLCDPEIILSMAEDELLRRGAVRNEDLIIMTIGEPVGKAGGTNTMKLVRVGGSRGTTQ
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCHH
ITKEVTQQTFSDPLNL
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]